Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6060 and GFF6061 overlap by 4 nucleotides GFF6061 and GFF6062 are separated by 64 nucleotides GFF6062 and GFF6063 are separated by 22 nucleotides
GFF6060 - ABC transporter, ATP-binding protein (cluster 1, maltose/g3p/polyamine/iron); ABC transporter, ATP-binding protein (cluster 10, nitrate/sulfonate/bicarbonate), at 121,544 to 122,608
GFF6060
GFF6061 - 3',5'-cyclic-nucleotide phosphodiesterase (EC 3.1.4.17), at 122,605 to 123,468
GFF6061
GFF6062 - Sodium-dependent phosphate transporter, at 123,533 to 125,194
GFF6062
GFF6063 - Sodium/bile acid symporter family, at 125,217 to 126,221
GFF6063
Position (kb)
123
124
125
126 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 122.689 kb on + strand at 122.689 kb on + strand at 122.690 kb on - strand at 122.690 kb on - strand at 122.713 kb on + strand, within GFF6061 at 122.812 kb on + strand, within GFF6061 at 122.813 kb on - strand, within GFF6061 at 122.857 kb on + strand, within GFF6061 at 122.857 kb on + strand, within GFF6061 at 122.857 kb on + strand, within GFF6061 at 122.920 kb on + strand, within GFF6061 at 122.920 kb on + strand, within GFF6061 at 122.920 kb on + strand, within GFF6061 at 122.921 kb on - strand, within GFF6061 at 122.921 kb on - strand, within GFF6061 at 122.921 kb on - strand, within GFF6061 at 123.417 kb on + strand at 123.518 kb on + strand at 123.543 kb on + strand at 123.543 kb on + strand at 123.543 kb on + strand at 123.543 kb on + strand at 123.544 kb on - strand at 123.544 kb on - strand at 123.544 kb on - strand at 123.544 kb on - strand at 123.544 kb on - strand at 123.544 kb on - strand at 123.544 kb on - strand at 123.583 kb on + strand at 123.598 kb on + strand at 123.615 kb on + strand at 123.616 kb on - strand at 123.822 kb on - strand, within GFF6062 at 124.319 kb on + strand, within GFF6062 at 124.319 kb on + strand, within GFF6062 at 124.319 kb on + strand, within GFF6062 at 124.319 kb on + strand, within GFF6062 at 124.320 kb on - strand, within GFF6062 at 124.320 kb on - strand, within GFF6062 at 124.667 kb on + strand, within GFF6062 at 124.667 kb on + strand, within GFF6062 at 124.668 kb on - strand, within GFF6062 at 124.668 kb on - strand, within GFF6062 at 124.668 kb on - strand, within GFF6062 at 124.682 kb on + strand, within GFF6062 at 124.682 kb on + strand, within GFF6062 at 124.682 kb on + strand, within GFF6062 at 124.683 kb on - strand, within GFF6062 at 124.683 kb on - strand, within GFF6062 at 124.683 kb on - strand, within GFF6062 at 124.685 kb on + strand, within GFF6062 at 124.685 kb on + strand, within GFF6062 at 124.685 kb on + strand, within GFF6062 at 124.685 kb on + strand, within GFF6062 at 124.685 kb on + strand, within GFF6062 at 124.685 kb on + strand, within GFF6062 at 124.686 kb on - strand, within GFF6062 at 124.686 kb on - strand, within GFF6062 at 124.686 kb on - strand, within GFF6062 at 124.686 kb on - strand, within GFF6062 at 124.686 kb on - strand, within GFF6062 at 124.686 kb on - strand, within GFF6062 at 125.175 kb on + strand at 125.175 kb on + strand at 125.175 kb on + strand at 125.175 kb on + strand at 125.175 kb on + strand at 125.176 kb on - strand at 125.218 kb on + strand at 125.218 kb on + strand at 125.218 kb on + strand at 125.219 kb on - strand at 125.219 kb on - strand at 125.272 kb on + strand at 125.273 kb on - strand at 125.273 kb on - strand at 125.587 kb on + strand, within GFF6063 at 125.587 kb on + strand, within GFF6063 at 125.587 kb on + strand, within GFF6063 at 125.587 kb on + strand, within GFF6063 at 125.587 kb on + strand, within GFF6063 at 125.587 kb on + strand, within GFF6063 at 125.587 kb on + strand, within GFF6063 at 125.588 kb on - strand, within GFF6063 at 125.588 kb on - strand, within GFF6063 at 125.588 kb on - strand, within GFF6063 at 125.588 kb on - strand, within GFF6063 at 125.588 kb on - strand, within GFF6063 at 125.737 kb on + strand, within GFF6063 at 125.923 kb on + strand, within GFF6063 at 125.923 kb on + strand, within GFF6063 at 125.924 kb on - strand, within GFF6063 at 125.924 kb on - strand, within GFF6063 at 125.924 kb on - strand, within GFF6063 at 125.924 kb on - strand, within GFF6063 at 125.924 kb on - strand, within GFF6063 at 125.924 kb on - strand, within GFF6063 at 125.924 kb on - strand, within GFF6063
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 122,689 + -0.8 122,689 + -0.9 122,690 - -1.5 122,690 - -0.7 122,713 + GFF6061 0.12 +1.9 122,812 + GFF6061 0.24 -0.3 122,813 - GFF6061 0.24 -0.1 122,857 + GFF6061 0.29 +1.1 122,857 + GFF6061 0.29 -1.0 122,857 + GFF6061 0.29 -0.7 122,920 + GFF6061 0.36 +0.6 122,920 + GFF6061 0.36 +0.1 122,920 + GFF6061 0.36 -1.4 122,921 - GFF6061 0.37 +0.1 122,921 - GFF6061 0.37 -0.7 122,921 - GFF6061 0.37 -0.0 123,417 + +0.8 123,518 + -1.0 123,543 + -0.2 123,543 + -0.3 123,543 + -0.1 123,543 + -1.2 123,544 - -0.9 123,544 - +0.1 123,544 - -2.9 123,544 - +0.1 123,544 - +0.4 123,544 - -0.2 123,544 - -0.4 123,583 + +0.7 123,598 + -0.3 123,615 + -0.5 123,616 - +0.2 123,822 - GFF6062 0.17 +2.4 124,319 + GFF6062 0.47 -0.5 124,319 + GFF6062 0.47 -0.2 124,319 + GFF6062 0.47 +0.6 124,319 + GFF6062 0.47 +1.0 124,320 - GFF6062 0.47 +0.8 124,320 - GFF6062 0.47 -0.4 124,667 + GFF6062 0.68 -1.1 124,667 + GFF6062 0.68 -0.2 124,668 - GFF6062 0.68 -1.6 124,668 - GFF6062 0.68 +0.5 124,668 - GFF6062 0.68 +1.2 124,682 + GFF6062 0.69 -0.9 124,682 + GFF6062 0.69 -0.3 124,682 + GFF6062 0.69 +1.7 124,683 - GFF6062 0.69 +1.4 124,683 - GFF6062 0.69 -1.1 124,683 - GFF6062 0.69 -1.6 124,685 + GFF6062 0.69 +0.2 124,685 + GFF6062 0.69 +1.1 124,685 + GFF6062 0.69 -0.1 124,685 + GFF6062 0.69 -2.0 124,685 + GFF6062 0.69 +0.9 124,685 + GFF6062 0.69 +0.3 124,686 - GFF6062 0.69 +0.4 124,686 - GFF6062 0.69 +0.5 124,686 - GFF6062 0.69 -0.6 124,686 - GFF6062 0.69 +0.1 124,686 - GFF6062 0.69 +0.3 124,686 - GFF6062 0.69 -0.6 125,175 + -0.4 125,175 + -0.9 125,175 + -0.1 125,175 + -0.3 125,175 + +1.1 125,176 - -1.2 125,218 + -0.0 125,218 + -3.1 125,218 + +0.9 125,219 - +0.5 125,219 - -0.3 125,272 + -0.4 125,273 - -1.5 125,273 - +1.4 125,587 + GFF6063 0.37 -0.8 125,587 + GFF6063 0.37 +1.0 125,587 + GFF6063 0.37 -0.8 125,587 + GFF6063 0.37 +0.2 125,587 + GFF6063 0.37 -0.0 125,587 + GFF6063 0.37 +0.8 125,587 + GFF6063 0.37 +0.2 125,588 - GFF6063 0.37 -0.9 125,588 - GFF6063 0.37 +0.1 125,588 - GFF6063 0.37 +0.1 125,588 - GFF6063 0.37 -0.2 125,588 - GFF6063 0.37 +0.7 125,737 + GFF6063 0.52 -0.1 125,923 + GFF6063 0.70 -0.5 125,923 + GFF6063 0.70 -0.5 125,924 - GFF6063 0.70 +0.0 125,924 - GFF6063 0.70 +0.3 125,924 - GFF6063 0.70 +0.0 125,924 - GFF6063 0.70 +0.4 125,924 - GFF6063 0.70 +2.2 125,924 - GFF6063 0.70 +0.7 125,924 - GFF6063 0.70 -0.3
Or see this region's nucleotide sequence