Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5943 and GFF5944 are separated by 27 nucleotides GFF5944 and GFF5945 are separated by 24 nucleotides
GFF5943 - hypothetical protein, at 164,230 to 165,393
GFF5943
GFF5944 - Adenylosuccinate lyase (EC 4.3.2.2), at 165,421 to 166,170
GFF5944
GFF5945 - Membrane-bound lytic murein transglycosylase A, at 166,195 to 167,796
GFF5945
Position (kb) 165
166
167 Strain fitness (log2 ratio) -1
0
1
2 at 164.519 kb on + strand, within GFF5943 at 164.520 kb on - strand, within GFF5943 at 165.414 kb on + strand at 165.415 kb on - strand at 165.415 kb on - strand at 165.415 kb on - strand at 165.428 kb on + strand at 165.429 kb on - strand at 165.603 kb on - strand, within GFF5944 at 165.879 kb on - strand, within GFF5944 at 165.890 kb on + strand, within GFF5944 at 165.983 kb on + strand, within GFF5944 at 165.983 kb on + strand, within GFF5944 at 165.983 kb on + strand, within GFF5944 at 165.984 kb on - strand, within GFF5944 at 165.984 kb on - strand, within GFF5944 at 166.076 kb on + strand, within GFF5944 at 166.076 kb on + strand, within GFF5944 at 166.407 kb on - strand, within GFF5945 at 166.515 kb on - strand, within GFF5945 at 166.628 kb on + strand, within GFF5945 at 166.628 kb on + strand, within GFF5945 at 166.628 kb on + strand, within GFF5945 at 166.628 kb on + strand, within GFF5945 at 166.629 kb on - strand, within GFF5945 at 166.629 kb on - strand, within GFF5945 at 166.629 kb on - strand, within GFF5945 at 166.629 kb on - strand, within GFF5945 at 166.892 kb on + strand, within GFF5945 at 167.036 kb on + strand, within GFF5945 at 167.037 kb on - strand, within GFF5945 at 167.037 kb on - strand, within GFF5945 at 167.039 kb on + strand, within GFF5945 at 167.039 kb on + strand, within GFF5945
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 164,519 + GFF5943 0.25 -0.7 164,520 - GFF5943 0.25 -0.5 165,414 + -1.2 165,415 - -0.0 165,415 - -0.2 165,415 - -1.5 165,428 + +0.1 165,429 - +0.1 165,603 - GFF5944 0.24 -0.1 165,879 - GFF5944 0.61 +0.8 165,890 + GFF5944 0.63 -0.2 165,983 + GFF5944 0.75 -0.9 165,983 + GFF5944 0.75 -0.9 165,983 + GFF5944 0.75 +0.0 165,984 - GFF5944 0.75 -1.6 165,984 - GFF5944 0.75 -1.6 166,076 + GFF5944 0.87 +0.0 166,076 + GFF5944 0.87 -0.4 166,407 - GFF5945 0.13 +1.8 166,515 - GFF5945 0.20 -0.6 166,628 + GFF5945 0.27 -0.7 166,628 + GFF5945 0.27 +0.8 166,628 + GFF5945 0.27 +0.0 166,628 + GFF5945 0.27 -1.4 166,629 - GFF5945 0.27 +0.8 166,629 - GFF5945 0.27 -0.5 166,629 - GFF5945 0.27 +0.3 166,629 - GFF5945 0.27 -1.4 166,892 + GFF5945 0.44 +0.2 167,036 + GFF5945 0.52 -0.2 167,037 - GFF5945 0.53 -0.1 167,037 - GFF5945 0.53 +0.2 167,039 + GFF5945 0.53 -0.3 167,039 + GFF5945 0.53 -0.1
Or see this region's nucleotide sequence