Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF591 and GFF592 are separated by 7 nucleotides GFF592 and GFF593 overlap by 1 nucleotides GFF593 and GFF594 are separated by 143 nucleotides
GFF591 - Renalase (EC 1.6.3.5), oxidases 1,2-dihydro- and 1,6-dihydro- beta-NAD(P)H isomers back to NAD(P), at 637,621 to 638,721
GFF591
GFF592 - Glutamyl-Q tRNA(Asp) synthetase, at 638,729 to 639,631
GFF592
GFF593 - tRNA (guanine(46)-N(7))-methyltransferase (EC 2.1.1.33), at 639,631 to 640,389
GFF593
GFF594 - ABC transporter, substrate-binding protein (cluster 4, leucine/isoleucine/valine/benzoate), at 640,533 to 641,654
GFF594
Position (kb)
638
639
640 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 637.858 kb on + strand, within GFF591 at 637.858 kb on + strand, within GFF591 at 637.859 kb on - strand, within GFF591 at 638.164 kb on + strand, within GFF591 at 638.164 kb on + strand, within GFF591 at 638.165 kb on - strand, within GFF591 at 638.165 kb on - strand, within GFF591 at 638.165 kb on - strand, within GFF591 at 638.305 kb on + strand, within GFF591 at 638.528 kb on + strand, within GFF591 at 638.529 kb on - strand, within GFF591 at 638.978 kb on + strand, within GFF592 at 638.978 kb on + strand, within GFF592 at 638.978 kb on + strand, within GFF592 at 638.978 kb on + strand, within GFF592 at 638.979 kb on - strand, within GFF592 at 638.979 kb on - strand, within GFF592 at 639.104 kb on + strand, within GFF592 at 639.284 kb on + strand, within GFF592 at 639.284 kb on + strand, within GFF592 at 639.285 kb on - strand, within GFF592 at 639.285 kb on - strand, within GFF592 at 639.285 kb on - strand, within GFF592 at 639.413 kb on + strand, within GFF592 at 639.413 kb on + strand, within GFF592 at 639.413 kb on + strand, within GFF592 at 639.414 kb on - strand, within GFF592 at 639.414 kb on - strand, within GFF592 at 639.414 kb on - strand, within GFF592 at 639.614 kb on + strand at 639.615 kb on - strand at 639.630 kb on - strand at 639.630 kb on - strand at 639.805 kb on + strand, within GFF593 at 639.805 kb on + strand, within GFF593 at 639.805 kb on + strand, within GFF593 at 639.805 kb on + strand, within GFF593 at 639.806 kb on - strand, within GFF593 at 639.806 kb on - strand, within GFF593 at 639.806 kb on - strand, within GFF593 at 639.806 kb on - strand, within GFF593 at 639.806 kb on - strand, within GFF593 at 639.817 kb on + strand, within GFF593 at 639.817 kb on + strand, within GFF593 at 639.818 kb on - strand, within GFF593 at 639.818 kb on - strand, within GFF593 at 639.818 kb on - strand, within GFF593 at 639.818 kb on - strand, within GFF593 at 640.195 kb on + strand, within GFF593 at 640.195 kb on + strand, within GFF593 at 640.195 kb on + strand, within GFF593 at 640.195 kb on + strand, within GFF593 at 640.195 kb on + strand, within GFF593 at 640.195 kb on + strand, within GFF593 at 640.196 kb on - strand, within GFF593 at 640.225 kb on + strand, within GFF593 at 640.225 kb on + strand, within GFF593 at 640.226 kb on - strand, within GFF593 at 640.288 kb on + strand, within GFF593 at 640.288 kb on + strand, within GFF593 at 640.288 kb on + strand, within GFF593 at 640.289 kb on - strand, within GFF593 at 640.289 kb on - strand, within GFF593 at 640.289 kb on - strand, within GFF593 at 640.289 kb on - strand, within GFF593 at 640.289 kb on - strand, within GFF593 at 640.307 kb on - strand, within GFF593 at 640.388 kb on - strand at 640.451 kb on - strand at 640.506 kb on + strand at 640.506 kb on + strand at 640.506 kb on + strand at 640.506 kb on + strand at 640.507 kb on - strand at 640.507 kb on - strand at 640.507 kb on - strand at 640.507 kb on - strand at 640.507 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 637,858 + GFF591 0.22 -0.6 637,858 + GFF591 0.22 -0.6 637,859 - GFF591 0.22 +0.4 638,164 + GFF591 0.49 -0.5 638,164 + GFF591 0.49 -0.2 638,165 - GFF591 0.49 -0.2 638,165 - GFF591 0.49 +0.1 638,165 - GFF591 0.49 -0.2 638,305 + GFF591 0.62 -0.2 638,528 + GFF591 0.82 +0.4 638,529 - GFF591 0.82 -0.0 638,978 + GFF592 0.28 -1.4 638,978 + GFF592 0.28 -0.3 638,978 + GFF592 0.28 -0.3 638,978 + GFF592 0.28 -0.4 638,979 - GFF592 0.28 -0.2 638,979 - GFF592 0.28 -0.5 639,104 + GFF592 0.42 -0.7 639,284 + GFF592 0.61 +0.8 639,284 + GFF592 0.61 +0.0 639,285 - GFF592 0.62 -0.5 639,285 - GFF592 0.62 +0.0 639,285 - GFF592 0.62 -1.8 639,413 + GFF592 0.76 -0.7 639,413 + GFF592 0.76 -0.9 639,413 + GFF592 0.76 -1.5 639,414 - GFF592 0.76 -0.5 639,414 - GFF592 0.76 -0.5 639,414 - GFF592 0.76 -1.7 639,614 + -0.6 639,615 - +0.7 639,630 - +0.6 639,630 - +0.2 639,805 + GFF593 0.23 +0.7 639,805 + GFF593 0.23 -0.2 639,805 + GFF593 0.23 -0.4 639,805 + GFF593 0.23 -1.6 639,806 - GFF593 0.23 -0.2 639,806 - GFF593 0.23 -0.3 639,806 - GFF593 0.23 +0.2 639,806 - GFF593 0.23 +1.4 639,806 - GFF593 0.23 -0.6 639,817 + GFF593 0.25 -0.2 639,817 + GFF593 0.25 -0.6 639,818 - GFF593 0.25 +1.2 639,818 - GFF593 0.25 -0.2 639,818 - GFF593 0.25 -1.6 639,818 - GFF593 0.25 +0.7 640,195 + GFF593 0.74 -0.4 640,195 + GFF593 0.74 -0.2 640,195 + GFF593 0.74 +1.0 640,195 + GFF593 0.74 -0.3 640,195 + GFF593 0.74 +0.5 640,195 + GFF593 0.74 -0.5 640,196 - GFF593 0.74 +0.1 640,225 + GFF593 0.78 +0.4 640,225 + GFF593 0.78 +0.2 640,226 - GFF593 0.78 -0.8 640,288 + GFF593 0.87 -1.5 640,288 + GFF593 0.87 +0.4 640,288 + GFF593 0.87 -1.1 640,289 - GFF593 0.87 -0.3 640,289 - GFF593 0.87 +0.2 640,289 - GFF593 0.87 -0.3 640,289 - GFF593 0.87 -0.0 640,289 - GFF593 0.87 +0.2 640,307 - GFF593 0.89 -0.9 640,388 - -1.2 640,451 - +1.9 640,506 + -1.7 640,506 + -0.3 640,506 + +0.8 640,506 + -1.3 640,507 - +0.2 640,507 - -0.6 640,507 - -0.5 640,507 - +0.3 640,507 - -0.4
Or see this region's nucleotide sequence