Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5778 and GFF5779 are separated by 16 nucleotides GFF5779 and GFF5780 are separated by 6 nucleotides
GFF5778 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 152,724 to 153,677
GFF5778
GFF5779 - L-idonate 5-dehydrogenase (EC 1.1.1.264), at 153,694 to 154,758
GFF5779
GFF5780 - Positive regulator of L-idonate catabolism, at 154,765 to 155,775
GFF5780
Position (kb)
153
154
155 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 152.770 kb on - strand at 152.770 kb on - strand at 152.805 kb on + strand at 152.805 kb on + strand at 152.806 kb on - strand at 152.806 kb on - strand at 152.806 kb on - strand at 152.806 kb on - strand at 152.955 kb on + strand, within GFF5778 at 152.956 kb on - strand, within GFF5778 at 152.956 kb on - strand, within GFF5778 at 153.785 kb on + strand at 153.785 kb on + strand at 153.785 kb on + strand at 153.786 kb on - strand at 153.786 kb on - strand at 153.786 kb on - strand at 153.786 kb on - strand at 153.832 kb on + strand, within GFF5779 at 153.832 kb on + strand, within GFF5779 at 153.833 kb on - strand, within GFF5779 at 153.833 kb on - strand, within GFF5779 at 154.039 kb on + strand, within GFF5779 at 154.039 kb on + strand, within GFF5779 at 154.039 kb on + strand, within GFF5779 at 154.040 kb on - strand, within GFF5779 at 154.040 kb on - strand, within GFF5779 at 154.393 kb on + strand, within GFF5779 at 154.653 kb on + strand at 154.654 kb on - strand at 155.108 kb on + strand, within GFF5780 at 155.486 kb on + strand, within GFF5780 at 155.639 kb on + strand, within GFF5780 at 155.639 kb on + strand, within GFF5780 at 155.639 kb on + strand, within GFF5780 at 155.640 kb on - strand, within GFF5780 at 155.640 kb on - strand, within GFF5780 at 155.640 kb on - strand, within GFF5780 at 155.640 kb on - strand, within GFF5780 at 155.640 kb on - strand, within GFF5780 at 155.640 kb on - strand, within GFF5780 at 155.640 kb on - strand, within GFF5780 at 155.640 kb on - strand, within GFF5780
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 152,770 - -0.0 152,770 - +0.9 152,805 + -0.5 152,805 + -1.1 152,806 - +0.1 152,806 - -0.7 152,806 - +0.3 152,806 - +0.9 152,955 + GFF5778 0.24 -2.0 152,956 - GFF5778 0.24 -0.1 152,956 - GFF5778 0.24 -0.0 153,785 + +0.3 153,785 + +0.3 153,785 + -0.3 153,786 - +0.2 153,786 - +0.8 153,786 - -0.4 153,786 - -1.0 153,832 + GFF5779 0.13 -0.5 153,832 + GFF5779 0.13 -0.4 153,833 - GFF5779 0.13 +1.8 153,833 - GFF5779 0.13 +2.3 154,039 + GFF5779 0.32 +0.7 154,039 + GFF5779 0.32 -0.2 154,039 + GFF5779 0.32 -0.9 154,040 - GFF5779 0.32 +0.6 154,040 - GFF5779 0.32 +1.2 154,393 + GFF5779 0.66 +0.1 154,653 + -0.4 154,654 - -1.4 155,108 + GFF5780 0.34 -0.8 155,486 + GFF5780 0.71 -1.4 155,639 + GFF5780 0.86 -0.2 155,639 + GFF5780 0.86 -0.4 155,639 + GFF5780 0.86 +0.9 155,640 - GFF5780 0.87 -0.2 155,640 - GFF5780 0.87 -0.6 155,640 - GFF5780 0.87 +0.2 155,640 - GFF5780 0.87 -0.7 155,640 - GFF5780 0.87 -1.2 155,640 - GFF5780 0.87 +0.8 155,640 - GFF5780 0.87 -1.3 155,640 - GFF5780 0.87 -0.4
Or see this region's nucleotide sequence