Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5660 and GFF5661 are separated by 90 nucleotides GFF5661 and GFF5662 are separated by 33 nucleotides GFF5662 and GFF5663 overlap by 4 nucleotides GFF5663 and GFF5664 are separated by 91 nucleotides
GFF5660 - Formate dehydrogenase O alpha subunit (EC 1.2.1.2) @ selenocysteine-containing, at 36,846 to 37,433
GFF5660
GFF5661 - hypothetical protein, at 37,524 to 37,823
GFF5661
GFF5662 - no description, at 37,857 to 38,027
GFF5662
GFF5663 - ABC transporter, fused permease protein, at 38,024 to 40,540
GFF5663
GFF5664 - hypothetical protein, at 40,632 to 42,236
GFF5664
Position (kb) 38
39
40
41 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 37.249 kb on + strand, within GFF5660 at 37.249 kb on + strand, within GFF5660 at 37.249 kb on + strand, within GFF5660 at 37.249 kb on + strand, within GFF5660 at 37.249 kb on + strand, within GFF5660 at 37.249 kb on + strand, within GFF5660 at 37.249 kb on + strand, within GFF5660 at 37.249 kb on + strand, within GFF5660 at 37.249 kb on + strand, within GFF5660 at 37.250 kb on - strand, within GFF5660 at 37.250 kb on - strand, within GFF5660 at 37.250 kb on - strand, within GFF5660 at 37.250 kb on - strand, within GFF5660 at 37.250 kb on - strand, within GFF5660 at 37.279 kb on + strand, within GFF5660 at 37.509 kb on + strand at 37.510 kb on - strand at 37.525 kb on + strand at 37.525 kb on + strand at 37.525 kb on + strand at 37.526 kb on - strand at 37.526 kb on - strand at 37.526 kb on - strand at 37.526 kb on - strand at 37.526 kb on - strand at 37.580 kb on + strand, within GFF5661 at 37.580 kb on + strand, within GFF5661 at 37.580 kb on + strand, within GFF5661 at 37.580 kb on + strand, within GFF5661 at 37.580 kb on + strand, within GFF5661 at 37.580 kb on + strand, within GFF5661 at 37.580 kb on + strand, within GFF5661 at 37.580 kb on + strand, within GFF5661 at 37.580 kb on + strand, within GFF5661 at 37.581 kb on - strand, within GFF5661 at 37.581 kb on - strand, within GFF5661 at 37.581 kb on - strand, within GFF5661 at 37.581 kb on - strand, within GFF5661 at 37.581 kb on - strand, within GFF5661 at 37.581 kb on - strand, within GFF5661 at 37.581 kb on - strand, within GFF5661 at 37.581 kb on - strand, within GFF5661 at 37.581 kb on - strand, within GFF5661 at 37.855 kb on + strand at 37.855 kb on + strand at 37.855 kb on + strand at 37.856 kb on - strand at 37.856 kb on - strand at 37.856 kb on - strand at 37.869 kb on + strand at 37.958 kb on + strand, within GFF5662 at 37.959 kb on - strand, within GFF5662 at 37.959 kb on - strand, within GFF5662 at 37.959 kb on - strand, within GFF5662 at 37.959 kb on - strand, within GFF5662 at 37.975 kb on + strand, within GFF5662 at 37.976 kb on - strand, within GFF5662 at 38.285 kb on + strand, within GFF5663 at 38.285 kb on + strand, within GFF5663 at 38.286 kb on - strand, within GFF5663 at 38.286 kb on - strand, within GFF5663 at 38.286 kb on - strand, within GFF5663 at 38.661 kb on - strand, within GFF5663 at 38.661 kb on - strand, within GFF5663 at 38.666 kb on + strand, within GFF5663 at 38.667 kb on - strand, within GFF5663 at 38.667 kb on - strand, within GFF5663 at 38.667 kb on - strand, within GFF5663 at 38.667 kb on - strand, within GFF5663 at 38.728 kb on - strand, within GFF5663 at 38.728 kb on - strand, within GFF5663 at 38.960 kb on + strand, within GFF5663 at 38.960 kb on + strand, within GFF5663 at 38.960 kb on + strand, within GFF5663 at 38.961 kb on - strand, within GFF5663 at 38.961 kb on - strand, within GFF5663 at 39.032 kb on + strand, within GFF5663 at 39.032 kb on + strand, within GFF5663 at 39.032 kb on + strand, within GFF5663 at 39.032 kb on + strand, within GFF5663 at 39.032 kb on + strand, within GFF5663 at 39.032 kb on + strand, within GFF5663 at 39.033 kb on - strand, within GFF5663 at 39.033 kb on - strand, within GFF5663 at 39.033 kb on - strand, within GFF5663 at 39.033 kb on - strand, within GFF5663 at 39.033 kb on - strand, within GFF5663 at 39.033 kb on - strand, within GFF5663 at 39.033 kb on - strand, within GFF5663 at 39.033 kb on - strand, within GFF5663 at 39.033 kb on - strand, within GFF5663 at 39.033 kb on - strand, within GFF5663 at 39.408 kb on + strand, within GFF5663 at 39.452 kb on + strand, within GFF5663 at 39.716 kb on + strand, within GFF5663 at 39.716 kb on + strand, within GFF5663 at 39.717 kb on - strand, within GFF5663 at 39.986 kb on + strand, within GFF5663 at 39.986 kb on + strand, within GFF5663 at 39.986 kb on + strand, within GFF5663 at 39.986 kb on + strand, within GFF5663 at 39.986 kb on + strand, within GFF5663 at 39.986 kb on + strand, within GFF5663 at 39.986 kb on + strand, within GFF5663 at 39.986 kb on + strand, within GFF5663 at 39.986 kb on + strand, within GFF5663 at 39.986 kb on + strand, within GFF5663 at 39.987 kb on - strand, within GFF5663 at 39.987 kb on - strand, within GFF5663 at 39.987 kb on - strand, within GFF5663 at 39.987 kb on - strand, within GFF5663 at 39.987 kb on - strand, within GFF5663 at 39.987 kb on - strand, within GFF5663 at 39.987 kb on - strand, within GFF5663 at 39.987 kb on - strand, within GFF5663 at 40.022 kb on + strand, within GFF5663 at 40.022 kb on + strand, within GFF5663 at 40.022 kb on + strand, within GFF5663 at 40.022 kb on + strand, within GFF5663 at 40.022 kb on + strand, within GFF5663 at 40.022 kb on + strand, within GFF5663 at 40.022 kb on + strand, within GFF5663 at 40.022 kb on + strand, within GFF5663 at 40.022 kb on + strand, within GFF5663 at 40.023 kb on - strand, within GFF5663 at 40.023 kb on - strand, within GFF5663 at 40.023 kb on - strand, within GFF5663 at 40.023 kb on - strand, within GFF5663 at 40.023 kb on - strand, within GFF5663 at 40.023 kb on - strand, within GFF5663 at 40.023 kb on - strand, within GFF5663 at 40.023 kb on - strand, within GFF5663 at 40.023 kb on - strand, within GFF5663 at 40.023 kb on - strand, within GFF5663 at 40.023 kb on - strand, within GFF5663 at 40.023 kb on - strand, within GFF5663 at 40.082 kb on + strand, within GFF5663 at 40.082 kb on + strand, within GFF5663 at 40.082 kb on + strand, within GFF5663 at 40.082 kb on + strand, within GFF5663 at 40.082 kb on + strand, within GFF5663 at 40.082 kb on + strand, within GFF5663 at 40.083 kb on - strand, within GFF5663 at 40.083 kb on - strand, within GFF5663 at 40.087 kb on + strand, within GFF5663 at 40.388 kb on + strand at 40.388 kb on + strand at 40.388 kb on + strand at 40.388 kb on + strand at 40.388 kb on + strand at 40.388 kb on + strand at 40.388 kb on + strand at 40.389 kb on - strand at 40.389 kb on - strand at 40.389 kb on - strand at 40.389 kb on - strand at 40.389 kb on - strand at 40.389 kb on - strand at 40.538 kb on + strand at 40.539 kb on - strand at 40.539 kb on - strand at 40.539 kb on - strand at 40.587 kb on + strand at 40.587 kb on + strand at 40.587 kb on + strand at 40.587 kb on + strand at 40.587 kb on + strand at 40.587 kb on + strand at 40.587 kb on + strand at 40.587 kb on + strand at 40.587 kb on + strand at 40.587 kb on + strand at 40.587 kb on + strand at 40.588 kb on - strand at 40.588 kb on - strand at 40.588 kb on - strand at 40.588 kb on - strand at 40.588 kb on - strand at 40.588 kb on - strand at 40.588 kb on - strand at 40.588 kb on - strand at 40.588 kb on - strand at 40.588 kb on - strand at 40.588 kb on - strand at 40.588 kb on - strand at 40.588 kb on - strand at 40.588 kb on - strand at 40.588 kb on - strand at 40.588 kb on - strand at 40.588 kb on - strand at 40.588 kb on - strand at 40.588 kb on - strand at 40.588 kb on - strand at 40.588 kb on - strand at 40.588 kb on - strand at 40.604 kb on + strand at 40.605 kb on - strand at 40.629 kb on + strand at 40.629 kb on + strand at 40.629 kb on + strand at 40.629 kb on + strand at 40.629 kb on + strand at 40.629 kb on + strand at 40.629 kb on + strand at 40.629 kb on + strand at 40.629 kb on + strand at 40.629 kb on + strand at 40.629 kb on + strand at 40.629 kb on + strand at 40.629 kb on + strand at 40.629 kb on + strand at 40.629 kb on + strand at 40.629 kb on + strand at 40.629 kb on + strand at 40.629 kb on + strand at 40.630 kb on - strand at 40.630 kb on - strand at 40.630 kb on - strand at 40.630 kb on - strand at 40.630 kb on - strand at 40.630 kb on - strand at 40.630 kb on - strand at 40.630 kb on - strand at 40.630 kb on - strand at 40.630 kb on - strand at 40.630 kb on - strand at 40.630 kb on - strand at 40.630 kb on - strand at 40.630 kb on - strand at 40.863 kb on - strand, within GFF5664 at 40.960 kb on - strand, within GFF5664 at 41.151 kb on + strand, within GFF5664 at 41.151 kb on + strand, within GFF5664 at 41.151 kb on + strand, within GFF5664 at 41.151 kb on + strand, within GFF5664 at 41.151 kb on + strand, within GFF5664 at 41.151 kb on + strand, within GFF5664 at 41.151 kb on + strand, within GFF5664 at 41.151 kb on + strand, within GFF5664 at 41.151 kb on + strand, within GFF5664 at 41.151 kb on + strand, within GFF5664 at 41.151 kb on + strand, within GFF5664 at 41.152 kb on - strand, within GFF5664 at 41.152 kb on - strand, within GFF5664 at 41.152 kb on - strand, within GFF5664 at 41.349 kb on + strand, within GFF5664 at 41.349 kb on + strand, within GFF5664 at 41.349 kb on + strand, within GFF5664 at 41.350 kb on - strand, within GFF5664 at 41.350 kb on - strand, within GFF5664 at 41.350 kb on - strand, within GFF5664 at 41.350 kb on - strand, within GFF5664 at 41.350 kb on - strand, within GFF5664 at 41.350 kb on - strand, within GFF5664 at 41.350 kb on - strand, within GFF5664 at 41.350 kb on - strand, within GFF5664 at 41.361 kb on + strand, within GFF5664 at 41.397 kb on + strand, within GFF5664 at 41.397 kb on + strand, within GFF5664 at 41.397 kb on + strand, within GFF5664 at 41.398 kb on - strand, within GFF5664 at 41.398 kb on - strand, within GFF5664 at 41.398 kb on - strand, within GFF5664 at 41.398 kb on - strand, within GFF5664 at 41.398 kb on - strand, within GFF5664
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 37,249 + GFF5660 0.69 -0.7 37,249 + GFF5660 0.69 -0.7 37,249 + GFF5660 0.69 +0.2 37,249 + GFF5660 0.69 -1.3 37,249 + GFF5660 0.69 -0.2 37,249 + GFF5660 0.69 -0.4 37,249 + GFF5660 0.69 -0.2 37,249 + GFF5660 0.69 -1.8 37,249 + GFF5660 0.69 +0.1 37,250 - GFF5660 0.69 -0.1 37,250 - GFF5660 0.69 +0.0 37,250 - GFF5660 0.69 -0.6 37,250 - GFF5660 0.69 -2.1 37,250 - GFF5660 0.69 +0.0 37,279 + GFF5660 0.74 +0.2 37,509 + -0.5 37,510 - -0.1 37,525 + -1.2 37,525 + -0.6 37,525 + +1.1 37,526 - -0.6 37,526 - -0.8 37,526 - -2.5 37,526 - -0.3 37,526 - +0.9 37,580 + GFF5661 0.19 -0.4 37,580 + GFF5661 0.19 +0.3 37,580 + GFF5661 0.19 -0.8 37,580 + GFF5661 0.19 +0.8 37,580 + GFF5661 0.19 -2.4 37,580 + GFF5661 0.19 -1.4 37,580 + GFF5661 0.19 -1.0 37,580 + GFF5661 0.19 +1.2 37,580 + GFF5661 0.19 +0.5 37,581 - GFF5661 0.19 -0.3 37,581 - GFF5661 0.19 +1.0 37,581 - GFF5661 0.19 +0.0 37,581 - GFF5661 0.19 -0.4 37,581 - GFF5661 0.19 -0.2 37,581 - GFF5661 0.19 -1.8 37,581 - GFF5661 0.19 +0.8 37,581 - GFF5661 0.19 -0.5 37,581 - GFF5661 0.19 +1.6 37,855 + -1.2 37,855 + +1.0 37,855 + -0.6 37,856 - +0.4 37,856 - +0.3 37,856 - -0.3 37,869 + +0.3 37,958 + GFF5662 0.59 -0.7 37,959 - GFF5662 0.60 +0.2 37,959 - GFF5662 0.60 +1.8 37,959 - GFF5662 0.60 -0.0 37,959 - GFF5662 0.60 -2.4 37,975 + GFF5662 0.69 -0.3 37,976 - GFF5662 0.70 -0.1 38,285 + GFF5663 0.10 -0.2 38,285 + GFF5663 0.10 -0.2 38,286 - GFF5663 0.10 +0.9 38,286 - GFF5663 0.10 +0.0 38,286 - GFF5663 0.10 +0.2 38,661 - GFF5663 0.25 -0.8 38,661 - GFF5663 0.25 -0.4 38,666 + GFF5663 0.26 -1.2 38,667 - GFF5663 0.26 +0.5 38,667 - GFF5663 0.26 +0.3 38,667 - GFF5663 0.26 -0.1 38,667 - GFF5663 0.26 -0.2 38,728 - GFF5663 0.28 -1.6 38,728 - GFF5663 0.28 +0.6 38,960 + GFF5663 0.37 -0.2 38,960 + GFF5663 0.37 +0.5 38,960 + GFF5663 0.37 +0.4 38,961 - GFF5663 0.37 -0.3 38,961 - GFF5663 0.37 -1.2 39,032 + GFF5663 0.40 -1.2 39,032 + GFF5663 0.40 +1.1 39,032 + GFF5663 0.40 -0.0 39,032 + GFF5663 0.40 -2.1 39,032 + GFF5663 0.40 -0.4 39,032 + GFF5663 0.40 +1.3 39,033 - GFF5663 0.40 -0.6 39,033 - GFF5663 0.40 -0.2 39,033 - GFF5663 0.40 -0.9 39,033 - GFF5663 0.40 +1.2 39,033 - GFF5663 0.40 +0.2 39,033 - GFF5663 0.40 -1.6 39,033 - GFF5663 0.40 -0.5 39,033 - GFF5663 0.40 +0.3 39,033 - GFF5663 0.40 -1.2 39,033 - GFF5663 0.40 -0.0 39,408 + GFF5663 0.55 -0.3 39,452 + GFF5663 0.57 -0.4 39,716 + GFF5663 0.67 +0.8 39,716 + GFF5663 0.67 -0.5 39,717 - GFF5663 0.67 -0.2 39,986 + GFF5663 0.78 -0.1 39,986 + GFF5663 0.78 -0.2 39,986 + GFF5663 0.78 +1.8 39,986 + GFF5663 0.78 -0.2 39,986 + GFF5663 0.78 -2.2 39,986 + GFF5663 0.78 +0.2 39,986 + GFF5663 0.78 +1.6 39,986 + GFF5663 0.78 +0.6 39,986 + GFF5663 0.78 -0.6 39,986 + GFF5663 0.78 -0.6 39,987 - GFF5663 0.78 -0.6 39,987 - GFF5663 0.78 +1.1 39,987 - GFF5663 0.78 +3.1 39,987 - GFF5663 0.78 -0.9 39,987 - GFF5663 0.78 -0.9 39,987 - GFF5663 0.78 +0.0 39,987 - GFF5663 0.78 -0.5 39,987 - GFF5663 0.78 +1.0 40,022 + GFF5663 0.79 -0.2 40,022 + GFF5663 0.79 -0.6 40,022 + GFF5663 0.79 -1.2 40,022 + GFF5663 0.79 +0.6 40,022 + GFF5663 0.79 -0.4 40,022 + GFF5663 0.79 -0.7 40,022 + GFF5663 0.79 -0.7 40,022 + GFF5663 0.79 -1.3 40,022 + GFF5663 0.79 -0.6 40,023 - GFF5663 0.79 -1.2 40,023 - GFF5663 0.79 +0.1 40,023 - GFF5663 0.79 -1.3 40,023 - GFF5663 0.79 +0.0 40,023 - GFF5663 0.79 -0.6 40,023 - GFF5663 0.79 -1.5 40,023 - GFF5663 0.79 -1.3 40,023 - GFF5663 0.79 -2.0 40,023 - GFF5663 0.79 -0.2 40,023 - GFF5663 0.79 -0.5 40,023 - GFF5663 0.79 -0.4 40,023 - GFF5663 0.79 +0.2 40,082 + GFF5663 0.82 +0.4 40,082 + GFF5663 0.82 -0.6 40,082 + GFF5663 0.82 -0.8 40,082 + GFF5663 0.82 +0.3 40,082 + GFF5663 0.82 -0.6 40,082 + GFF5663 0.82 -0.6 40,083 - GFF5663 0.82 +0.3 40,083 - GFF5663 0.82 -0.9 40,087 + GFF5663 0.82 -0.1 40,388 + -0.5 40,388 + +0.2 40,388 + +0.2 40,388 + -1.3 40,388 + -0.6 40,388 + +0.6 40,388 + -1.6 40,389 - -0.4 40,389 - -2.8 40,389 - -0.5 40,389 - +0.4 40,389 - -1.1 40,389 - -2.3 40,538 + +1.2 40,539 - -0.6 40,539 - -0.1 40,539 - -0.7 40,587 + +0.7 40,587 + -0.1 40,587 + -1.6 40,587 + +0.8 40,587 + +1.2 40,587 + -0.4 40,587 + -0.1 40,587 + -1.8 40,587 + +0.1 40,587 + -1.0 40,587 + -0.2 40,588 - -1.1 40,588 - -0.6 40,588 - +0.8 40,588 - +0.9 40,588 - -0.2 40,588 - -0.8 40,588 - +1.8 40,588 - +0.1 40,588 - -2.4 40,588 - +0.5 40,588 - -0.7 40,588 - +1.4 40,588 - +0.8 40,588 - -1.1 40,588 - +0.2 40,588 - -2.2 40,588 - -0.5 40,588 - +1.2 40,588 - -0.6 40,588 - +0.5 40,588 - -0.1 40,588 - -0.1 40,604 + -0.5 40,605 - -0.6 40,629 + -2.8 40,629 + -0.4 40,629 + +0.2 40,629 + +0.4 40,629 + -1.2 40,629 + -1.2 40,629 + +0.8 40,629 + +0.0 40,629 + +0.2 40,629 + +0.2 40,629 + -0.7 40,629 + -0.2 40,629 + +0.1 40,629 + +2.8 40,629 + -0.1 40,629 + -0.2 40,629 + -0.5 40,629 + -0.2 40,630 - +0.4 40,630 - -0.2 40,630 - -1.0 40,630 - +0.4 40,630 - -0.4 40,630 - -0.3 40,630 - +0.2 40,630 - -1.0 40,630 - -0.4 40,630 - -0.5 40,630 - +0.3 40,630 - +0.5 40,630 - -0.7 40,630 - -0.8 40,863 - GFF5664 0.14 +0.7 40,960 - GFF5664 0.20 -0.8 41,151 + GFF5664 0.32 -0.6 41,151 + GFF5664 0.32 +0.0 41,151 + GFF5664 0.32 -0.6 41,151 + GFF5664 0.32 -0.6 41,151 + GFF5664 0.32 +1.2 41,151 + GFF5664 0.32 -0.9 41,151 + GFF5664 0.32 +1.2 41,151 + GFF5664 0.32 -0.1 41,151 + GFF5664 0.32 +1.4 41,151 + GFF5664 0.32 -0.1 41,151 + GFF5664 0.32 -0.2 41,152 - GFF5664 0.32 -1.2 41,152 - GFF5664 0.32 +0.3 41,152 - GFF5664 0.32 -0.6 41,349 + GFF5664 0.45 +0.7 41,349 + GFF5664 0.45 -0.5 41,349 + GFF5664 0.45 -0.2 41,350 - GFF5664 0.45 -0.4 41,350 - GFF5664 0.45 +1.8 41,350 - GFF5664 0.45 -1.1 41,350 - GFF5664 0.45 -2.1 41,350 - GFF5664 0.45 -1.3 41,350 - GFF5664 0.45 -0.8 41,350 - GFF5664 0.45 +2.2 41,350 - GFF5664 0.45 +1.2 41,361 + GFF5664 0.45 +0.4 41,397 + GFF5664 0.48 +0.1 41,397 + GFF5664 0.48 +0.8 41,397 + GFF5664 0.48 +4.3 41,398 - GFF5664 0.48 -0.5 41,398 - GFF5664 0.48 +0.5 41,398 - GFF5664 0.48 -0.1 41,398 - GFF5664 0.48 +0.2 41,398 - GFF5664 0.48 -0.1
Or see this region's nucleotide sequence