Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5646 and GFF5647 overlap by 4 nucleotides GFF5647 and GFF5648 are separated by 10 nucleotides GFF5648 and GFF5649 are separated by 74 nucleotides
GFF5646 - ABC transporter, permease protein (cluster 12, methionine/phosphonates) / ABC transporter, permease protein (cluster 12, methionine/phosphonates), at 21,198 to 22,037
GFF5646
GFF5647 - ABC transporter, ATP-binding protein (cluster 12, methionine/phosphonates), at 22,034 to 22,846
GFF5647
GFF5648 - ABC transporter, substrate-binding protein (cluster 12, methionine/phosphonates), at 22,857 to 23,717
GFF5648
GFF5649 - Selenide,water dikinase (EC 2.7.9.3), at 23,792 to 24,859
GFF5649
Position (kb) 22
23
24 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 22.119 kb on + strand, within GFF5647 at 22.119 kb on + strand, within GFF5647 at 22.119 kb on + strand, within GFF5647 at 22.120 kb on - strand, within GFF5647 at 22.362 kb on + strand, within GFF5647 at 22.363 kb on - strand, within GFF5647 at 22.363 kb on - strand, within GFF5647 at 22.363 kb on - strand, within GFF5647 at 22.363 kb on - strand, within GFF5647 at 22.903 kb on + strand at 22.903 kb on + strand at 22.903 kb on + strand at 22.903 kb on + strand at 22.903 kb on + strand at 22.903 kb on + strand at 22.904 kb on - strand at 22.904 kb on - strand at 22.904 kb on - strand at 23.053 kb on + strand, within GFF5648 at 23.053 kb on + strand, within GFF5648 at 23.053 kb on + strand, within GFF5648 at 23.054 kb on - strand, within GFF5648 at 23.054 kb on - strand, within GFF5648 at 23.059 kb on + strand, within GFF5648 at 23.059 kb on + strand, within GFF5648 at 23.059 kb on + strand, within GFF5648 at 23.059 kb on + strand, within GFF5648 at 23.060 kb on - strand, within GFF5648 at 23.060 kb on - strand, within GFF5648 at 23.060 kb on - strand, within GFF5648 at 23.062 kb on + strand, within GFF5648 at 23.062 kb on + strand, within GFF5648 at 23.062 kb on + strand, within GFF5648 at 23.062 kb on + strand, within GFF5648 at 23.062 kb on + strand, within GFF5648 at 23.062 kb on + strand, within GFF5648 at 23.062 kb on + strand, within GFF5648 at 23.062 kb on + strand, within GFF5648 at 23.063 kb on - strand, within GFF5648 at 23.063 kb on - strand, within GFF5648 at 23.063 kb on - strand, within GFF5648 at 23.063 kb on - strand, within GFF5648 at 23.063 kb on - strand, within GFF5648 at 23.264 kb on - strand, within GFF5648 at 23.264 kb on - strand, within GFF5648 at 23.566 kb on + strand, within GFF5648 at 23.566 kb on + strand, within GFF5648 at 23.743 kb on + strand at 23.761 kb on + strand at 23.761 kb on + strand at 23.761 kb on + strand at 23.762 kb on - strand at 23.762 kb on - strand at 23.762 kb on - strand at 23.972 kb on + strand, within GFF5649 at 23.972 kb on + strand, within GFF5649 at 23.973 kb on - strand, within GFF5649 at 24.041 kb on + strand, within GFF5649 at 24.042 kb on - strand, within GFF5649 at 24.086 kb on + strand, within GFF5649 at 24.086 kb on + strand, within GFF5649 at 24.086 kb on + strand, within GFF5649 at 24.086 kb on + strand, within GFF5649 at 24.087 kb on - strand, within GFF5649 at 24.087 kb on - strand, within GFF5649 at 24.087 kb on - strand, within GFF5649 at 24.087 kb on - strand, within GFF5649 at 24.254 kb on + strand, within GFF5649 at 24.255 kb on - strand, within GFF5649 at 24.255 kb on - strand, within GFF5649 at 24.255 kb on - strand, within GFF5649
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 22,119 + GFF5647 0.10 +2.1 22,119 + GFF5647 0.10 -1.1 22,119 + GFF5647 0.10 -1.2 22,120 - GFF5647 0.11 -0.7 22,362 + GFF5647 0.40 -0.1 22,363 - GFF5647 0.40 -0.1 22,363 - GFF5647 0.40 -0.0 22,363 - GFF5647 0.40 -0.6 22,363 - GFF5647 0.40 +0.5 22,903 + -0.0 22,903 + -0.7 22,903 + +0.2 22,903 + +0.2 22,903 + -0.6 22,903 + -0.2 22,904 - -2.3 22,904 - -0.4 22,904 - +0.2 23,053 + GFF5648 0.23 +0.2 23,053 + GFF5648 0.23 +0.5 23,053 + GFF5648 0.23 -0.8 23,054 - GFF5648 0.23 -1.2 23,054 - GFF5648 0.23 +0.8 23,059 + GFF5648 0.23 +0.3 23,059 + GFF5648 0.23 -1.3 23,059 + GFF5648 0.23 -0.5 23,059 + GFF5648 0.23 -0.4 23,060 - GFF5648 0.24 +0.5 23,060 - GFF5648 0.24 -0.1 23,060 - GFF5648 0.24 -1.2 23,062 + GFF5648 0.24 +0.1 23,062 + GFF5648 0.24 +2.3 23,062 + GFF5648 0.24 -0.1 23,062 + GFF5648 0.24 +0.1 23,062 + GFF5648 0.24 -0.3 23,062 + GFF5648 0.24 -2.1 23,062 + GFF5648 0.24 -0.9 23,062 + GFF5648 0.24 +0.0 23,063 - GFF5648 0.24 +0.3 23,063 - GFF5648 0.24 +0.5 23,063 - GFF5648 0.24 +1.3 23,063 - GFF5648 0.24 -0.6 23,063 - GFF5648 0.24 +0.2 23,264 - GFF5648 0.47 +0.5 23,264 - GFF5648 0.47 -0.5 23,566 + GFF5648 0.82 +2.2 23,566 + GFF5648 0.82 +0.5 23,743 + +1.2 23,761 + -0.3 23,761 + +0.1 23,761 + -0.7 23,762 - -0.6 23,762 - +0.4 23,762 - -0.6 23,972 + GFF5649 0.17 -2.9 23,972 + GFF5649 0.17 -1.5 23,973 - GFF5649 0.17 -0.3 24,041 + GFF5649 0.23 +0.4 24,042 - GFF5649 0.23 +0.1 24,086 + GFF5649 0.28 -0.9 24,086 + GFF5649 0.28 -1.8 24,086 + GFF5649 0.28 -0.5 24,086 + GFF5649 0.28 -0.3 24,087 - GFF5649 0.28 +0.1 24,087 - GFF5649 0.28 -0.7 24,087 - GFF5649 0.28 -0.6 24,087 - GFF5649 0.28 -0.2 24,254 + GFF5649 0.43 +0.3 24,255 - GFF5649 0.43 +0.2 24,255 - GFF5649 0.43 +0.1 24,255 - GFF5649 0.43 +0.3
Or see this region's nucleotide sequence