Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5398 and GFF5399 are separated by 14 nucleotides GFF5399 and GFF5400 are separated by 11 nucleotides GFF5400 and GFF5401 are separated by 230 nucleotides
GFF5398 - L-fuconate dehydratase (EC 4.2.1.68), type 2, at 22,697 to 23,929
GFF5398
GFF5399 - Altronate dehydratase (EC 4.2.1.7), at 23,944 to 24,258
GFF5399
GFF5400 - Demethylmenaquinone methyltransferase-like protein, at 24,270 to 24,944
GFF5400
GFF5401 - 2-keto-3-deoxy-L-fuconate dehydrogenase, at 25,175 to 25,945
GFF5401
Position (kb)
24
25 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 23.733 kb on + strand, within GFF5398 at 23.733 kb on + strand, within GFF5398 at 23.734 kb on - strand, within GFF5398 at 23.745 kb on + strand, within GFF5398 at 23.847 kb on + strand at 23.847 kb on + strand at 23.848 kb on - strand at 23.848 kb on - strand at 23.961 kb on - strand at 23.984 kb on + strand, within GFF5399 at 23.984 kb on + strand, within GFF5399 at 23.984 kb on + strand, within GFF5399 at 23.985 kb on - strand, within GFF5399 at 23.985 kb on - strand, within GFF5399 at 23.985 kb on - strand, within GFF5399 at 23.985 kb on - strand, within GFF5399 at 24.062 kb on + strand, within GFF5399 at 24.062 kb on + strand, within GFF5399 at 24.062 kb on + strand, within GFF5399 at 24.062 kb on + strand, within GFF5399 at 24.062 kb on + strand, within GFF5399 at 24.063 kb on - strand, within GFF5399 at 24.283 kb on + strand at 24.292 kb on + strand at 24.292 kb on + strand at 24.292 kb on + strand at 24.292 kb on + strand at 24.292 kb on + strand at 24.293 kb on - strand at 24.293 kb on - strand at 24.293 kb on - strand at 24.293 kb on - strand at 24.493 kb on - strand, within GFF5400 at 24.499 kb on + strand, within GFF5400 at 24.500 kb on - strand, within GFF5400 at 24.500 kb on - strand, within GFF5400 at 24.526 kb on + strand, within GFF5400 at 24.526 kb on + strand, within GFF5400 at 24.526 kb on + strand, within GFF5400 at 24.526 kb on + strand, within GFF5400 at 24.527 kb on - strand, within GFF5400 at 24.527 kb on - strand, within GFF5400 at 24.527 kb on - strand, within GFF5400 at 24.527 kb on - strand, within GFF5400 at 24.883 kb on + strand at 24.883 kb on + strand at 24.884 kb on - strand at 24.884 kb on - strand at 24.884 kb on - strand at 24.884 kb on - strand at 24.884 kb on - strand at 24.884 kb on - strand at 24.973 kb on + strand at 25.008 kb on + strand at 25.008 kb on + strand at 25.008 kb on + strand at 25.008 kb on + strand at 25.008 kb on + strand at 25.008 kb on + strand at 25.009 kb on - strand at 25.009 kb on - strand at 25.009 kb on - strand at 25.009 kb on - strand at 25.009 kb on - strand at 25.009 kb on - strand at 25.009 kb on - strand at 25.009 kb on - strand at 25.009 kb on - strand at 25.030 kb on + strand at 25.030 kb on + strand at 25.030 kb on + strand at 25.030 kb on + strand at 25.030 kb on + strand at 25.030 kb on + strand at 25.031 kb on - strand at 25.031 kb on - strand at 25.031 kb on - strand at 25.031 kb on - strand at 25.031 kb on - strand at 25.031 kb on - strand at 25.031 kb on - strand at 25.031 kb on - strand at 25.031 kb on - strand at 25.040 kb on + strand at 25.040 kb on + strand at 25.040 kb on + strand at 25.041 kb on - strand at 25.041 kb on - strand at 25.041 kb on - strand at 25.105 kb on + strand at 25.106 kb on - strand at 25.107 kb on + strand at 25.107 kb on + strand at 25.107 kb on + strand at 25.107 kb on + strand at 25.108 kb on - strand at 25.128 kb on + strand at 25.389 kb on + strand, within GFF5401 at 25.389 kb on + strand, within GFF5401 at 25.389 kb on + strand, within GFF5401 at 25.390 kb on - strand, within GFF5401 at 25.390 kb on - strand, within GFF5401 at 25.390 kb on - strand, within GFF5401 at 25.390 kb on - strand, within GFF5401 at 25.462 kb on + strand, within GFF5401 at 25.462 kb on + strand, within GFF5401 at 25.462 kb on + strand, within GFF5401 at 25.523 kb on + strand, within GFF5401 at 25.523 kb on + strand, within GFF5401 at 25.523 kb on + strand, within GFF5401 at 25.523 kb on + strand, within GFF5401 at 25.524 kb on - strand, within GFF5401 at 25.524 kb on - strand, within GFF5401 at 25.524 kb on - strand, within GFF5401 at 25.524 kb on - strand, within GFF5401 at 25.524 kb on - strand, within GFF5401 at 25.524 kb on - strand, within GFF5401 at 25.631 kb on + strand, within GFF5401 at 25.874 kb on + strand at 25.874 kb on + strand at 25.874 kb on + strand at 25.874 kb on + strand at 25.874 kb on + strand at 25.874 kb on + strand at 25.875 kb on - strand at 25.875 kb on - strand at 25.875 kb on - strand at 25.875 kb on - strand at 25.875 kb on - strand at 25.875 kb on - strand at 25.875 kb on - strand at 25.875 kb on - strand at 25.918 kb on + strand at 25.918 kb on + strand at 25.918 kb on + strand at 25.918 kb on + strand at 25.919 kb on - strand at 25.919 kb on - strand at 25.919 kb on - strand at 25.919 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 23,733 + GFF5398 0.84 -0.4 23,733 + GFF5398 0.84 +0.1 23,734 - GFF5398 0.84 +1.3 23,745 + GFF5398 0.85 -0.1 23,847 + -0.1 23,847 + -0.0 23,848 - -0.8 23,848 - -0.8 23,961 - +0.2 23,984 + GFF5399 0.13 +0.9 23,984 + GFF5399 0.13 -0.9 23,984 + GFF5399 0.13 -0.5 23,985 - GFF5399 0.13 -0.3 23,985 - GFF5399 0.13 -0.2 23,985 - GFF5399 0.13 -0.5 23,985 - GFF5399 0.13 -0.8 24,062 + GFF5399 0.37 -1.5 24,062 + GFF5399 0.37 -0.1 24,062 + GFF5399 0.37 +0.6 24,062 + GFF5399 0.37 -0.4 24,062 + GFF5399 0.37 +0.7 24,063 - GFF5399 0.38 -1.3 24,283 + +0.2 24,292 + -2.0 24,292 + -0.5 24,292 + -0.2 24,292 + -1.0 24,292 + -1.1 24,293 - +0.1 24,293 - +0.1 24,293 - -0.6 24,293 - -0.2 24,493 - GFF5400 0.33 -0.4 24,499 + GFF5400 0.34 +0.1 24,500 - GFF5400 0.34 -0.1 24,500 - GFF5400 0.34 +0.3 24,526 + GFF5400 0.38 -0.5 24,526 + GFF5400 0.38 +1.3 24,526 + GFF5400 0.38 +0.8 24,526 + GFF5400 0.38 +1.0 24,527 - GFF5400 0.38 -1.6 24,527 - GFF5400 0.38 +0.3 24,527 - GFF5400 0.38 +1.2 24,527 - GFF5400 0.38 -0.8 24,883 + +0.1 24,883 + -0.6 24,884 - -0.2 24,884 - -0.2 24,884 - +0.2 24,884 - +1.1 24,884 - -0.0 24,884 - +1.1 24,973 + -0.0 25,008 + -0.5 25,008 + -1.3 25,008 + -0.4 25,008 + -0.6 25,008 + +0.2 25,008 + -0.5 25,009 - -0.2 25,009 - -1.1 25,009 - -0.1 25,009 - -0.5 25,009 - -0.9 25,009 - -0.3 25,009 - -0.0 25,009 - -1.3 25,009 - -0.3 25,030 + -2.1 25,030 + -0.4 25,030 + +0.5 25,030 + -0.5 25,030 + -0.2 25,030 + -0.2 25,031 - -0.2 25,031 - -0.0 25,031 - +1.1 25,031 - -0.2 25,031 - +1.9 25,031 - -1.2 25,031 - +0.1 25,031 - -1.9 25,031 - -0.3 25,040 + -0.9 25,040 + -1.1 25,040 + -0.1 25,041 - +0.1 25,041 - -1.0 25,041 - -0.4 25,105 + +0.9 25,106 - +0.9 25,107 + +0.0 25,107 + -0.4 25,107 + +0.1 25,107 + -0.1 25,108 - -0.3 25,128 + -0.3 25,389 + GFF5401 0.28 +0.6 25,389 + GFF5401 0.28 -0.1 25,389 + GFF5401 0.28 -1.3 25,390 - GFF5401 0.28 +0.1 25,390 - GFF5401 0.28 -0.2 25,390 - GFF5401 0.28 -0.6 25,390 - GFF5401 0.28 -0.7 25,462 + GFF5401 0.37 -0.8 25,462 + GFF5401 0.37 -0.2 25,462 + GFF5401 0.37 +0.4 25,523 + GFF5401 0.45 -0.2 25,523 + GFF5401 0.45 +0.5 25,523 + GFF5401 0.45 -0.4 25,523 + GFF5401 0.45 +0.1 25,524 - GFF5401 0.45 -0.8 25,524 - GFF5401 0.45 -0.4 25,524 - GFF5401 0.45 -0.0 25,524 - GFF5401 0.45 -0.4 25,524 - GFF5401 0.45 +0.3 25,524 - GFF5401 0.45 -0.0 25,631 + GFF5401 0.59 +0.0 25,874 + +0.6 25,874 + -0.1 25,874 + -0.4 25,874 + -0.4 25,874 + -0.4 25,874 + -0.0 25,875 - +0.2 25,875 - -0.6 25,875 - +0.1 25,875 - +1.3 25,875 - -0.2 25,875 - -1.4 25,875 - +0.6 25,875 - +3.5 25,918 + -1.3 25,918 + -2.9 25,918 + -3.2 25,918 + -0.0 25,919 - -0.8 25,919 - +0.6 25,919 - -0.2 25,919 - -0.7
Or see this region's nucleotide sequence