Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5180 and GFF5181 are separated by 105 nucleotides GFF5181 and GFF5182 are separated by 732 nucleotides GFF5182 and GFF5183 are separated by 0 nucleotides GFF5183 and GFF5184 are separated by 433 nucleotides
GFF5180 - NADH:flavin oxidoreductases, Old Yellow Enzyme family, at 33,331 to 34,464
GFF5180
GFF5181 - hypothetical protein, at 34,570 to 34,683
GFF5181
GFF5182 - no description, at 35,416 to 36,468
GFF5182
GFF5183 - no description, at 36,469 to 36,603
GFF5183
GFF5184 - no description, at 37,037 to 37,342
GFF5184
Position (kb)
35
36
37 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 34.609 kb on - strand, within GFF5181 at 34.609 kb on - strand, within GFF5181 at 34.772 kb on + strand at 34.773 kb on - strand at 34.905 kb on + strand at 34.905 kb on + strand at 34.929 kb on + strand at 34.949 kb on + strand at 34.950 kb on - strand at 35.005 kb on - strand at 35.005 kb on - strand at 35.005 kb on - strand at 35.007 kb on - strand at 35.027 kb on + strand at 35.133 kb on + strand at 35.133 kb on + strand at 35.134 kb on - strand at 35.134 kb on - strand at 35.134 kb on - strand at 35.134 kb on - strand at 35.417 kb on + strand at 35.417 kb on + strand at 35.418 kb on - strand at 35.418 kb on - strand at 35.482 kb on + strand at 35.482 kb on + strand at 35.482 kb on + strand at 35.483 kb on - strand at 35.483 kb on - strand at 35.483 kb on - strand at 35.483 kb on - strand at 35.483 kb on - strand at 35.483 kb on - strand at 35.483 kb on - strand at 35.483 kb on - strand at 35.531 kb on + strand, within GFF5182 at 35.579 kb on + strand, within GFF5182 at 35.580 kb on - strand, within GFF5182 at 35.580 kb on - strand, within GFF5182 at 35.580 kb on - strand, within GFF5182 at 35.580 kb on - strand, within GFF5182 at 35.580 kb on - strand, within GFF5182 at 35.580 kb on - strand, within GFF5182 at 35.580 kb on - strand, within GFF5182 at 35.633 kb on + strand, within GFF5182 at 35.634 kb on - strand, within GFF5182 at 35.634 kb on - strand, within GFF5182 at 35.634 kb on - strand, within GFF5182 at 35.735 kb on + strand, within GFF5182 at 35.735 kb on + strand, within GFF5182 at 35.735 kb on + strand, within GFF5182 at 35.736 kb on - strand, within GFF5182 at 35.736 kb on - strand, within GFF5182 at 35.736 kb on - strand, within GFF5182 at 35.758 kb on - strand, within GFF5182 at 35.922 kb on + strand, within GFF5182 at 35.922 kb on + strand, within GFF5182 at 35.922 kb on + strand, within GFF5182 at 35.923 kb on - strand, within GFF5182 at 35.925 kb on + strand, within GFF5182 at 35.926 kb on - strand, within GFF5182 at 35.926 kb on - strand, within GFF5182 at 35.968 kb on - strand, within GFF5182 at 36.086 kb on + strand, within GFF5182 at 36.086 kb on + strand, within GFF5182 at 36.087 kb on - strand, within GFF5182 at 36.121 kb on - strand, within GFF5182 at 36.121 kb on - strand, within GFF5182 at 36.126 kb on + strand, within GFF5182 at 36.126 kb on + strand, within GFF5182 at 36.126 kb on + strand, within GFF5182 at 36.127 kb on - strand, within GFF5182 at 36.127 kb on - strand, within GFF5182 at 36.127 kb on - strand, within GFF5182 at 36.127 kb on - strand, within GFF5182 at 36.127 kb on - strand, within GFF5182 at 36.127 kb on - strand, within GFF5182 at 36.127 kb on - strand, within GFF5182 at 36.128 kb on + strand, within GFF5182 at 36.128 kb on + strand, within GFF5182 at 36.128 kb on + strand, within GFF5182 at 36.128 kb on + strand, within GFF5182 at 36.128 kb on + strand, within GFF5182 at 36.128 kb on + strand, within GFF5182 at 36.128 kb on + strand, within GFF5182 at 36.128 kb on + strand, within GFF5182 at 36.129 kb on - strand, within GFF5182 at 36.129 kb on - strand, within GFF5182 at 36.129 kb on - strand, within GFF5182 at 36.129 kb on - strand, within GFF5182 at 36.129 kb on - strand, within GFF5182 at 36.129 kb on - strand, within GFF5182 at 36.129 kb on - strand, within GFF5182 at 36.129 kb on - strand, within GFF5182 at 36.129 kb on - strand, within GFF5182 at 36.129 kb on - strand, within GFF5182 at 36.171 kb on + strand, within GFF5182 at 36.270 kb on - strand, within GFF5182 at 36.270 kb on - strand, within GFF5182 at 36.287 kb on + strand, within GFF5182 at 36.287 kb on + strand, within GFF5182 at 36.287 kb on + strand, within GFF5182 at 36.288 kb on - strand, within GFF5182 at 36.288 kb on - strand, within GFF5182 at 36.359 kb on + strand, within GFF5182 at 36.417 kb on + strand at 36.417 kb on + strand at 36.417 kb on + strand at 36.418 kb on - strand at 36.418 kb on - strand at 36.418 kb on - strand at 36.418 kb on - strand at 36.418 kb on - strand at 36.423 kb on + strand at 36.424 kb on - strand at 36.470 kb on + strand at 36.470 kb on + strand at 36.474 kb on + strand at 36.589 kb on + strand, within GFF5183 at 36.589 kb on + strand, within GFF5183 at 36.656 kb on + strand at 36.735 kb on + strand at 36.735 kb on + strand at 36.735 kb on + strand at 36.735 kb on + strand at 36.735 kb on + strand at 36.735 kb on + strand at 36.735 kb on + strand at 36.736 kb on - strand at 36.736 kb on - strand at 36.736 kb on - strand at 36.736 kb on - strand at 36.736 kb on - strand at 36.736 kb on - strand at 36.741 kb on + strand at 36.741 kb on + strand at 36.741 kb on + strand at 36.742 kb on - strand at 36.742 kb on - strand at 36.767 kb on + strand at 36.767 kb on + strand at 36.767 kb on + strand at 36.767 kb on + strand at 36.768 kb on - strand at 36.768 kb on - strand at 36.906 kb on + strand at 36.906 kb on + strand at 36.964 kb on + strand at 36.964 kb on + strand at 36.964 kb on + strand at 36.964 kb on + strand at 36.964 kb on + strand at 36.964 kb on + strand at 36.965 kb on - strand at 36.965 kb on - strand at 36.965 kb on - strand at 36.965 kb on - strand at 36.983 kb on + strand at 36.983 kb on + strand at 36.983 kb on + strand at 36.983 kb on + strand at 36.983 kb on + strand at 36.984 kb on - strand at 36.984 kb on - strand at 36.984 kb on - strand at 36.984 kb on - strand at 37.021 kb on - strand at 37.030 kb on + strand at 37.030 kb on + strand at 37.031 kb on - strand at 37.038 kb on + strand at 37.038 kb on + strand at 37.038 kb on + strand at 37.038 kb on + strand at 37.038 kb on + strand at 37.038 kb on + strand at 37.039 kb on - strand at 37.039 kb on - strand at 37.039 kb on - strand at 37.039 kb on - strand at 37.074 kb on + strand, within GFF5184 at 37.075 kb on - strand, within GFF5184 at 37.075 kb on - strand, within GFF5184 at 37.090 kb on + strand, within GFF5184 at 37.178 kb on - strand, within GFF5184 at 37.232 kb on + strand, within GFF5184 at 37.233 kb on - strand, within GFF5184 at 37.233 kb on - strand, within GFF5184 at 37.233 kb on - strand, within GFF5184 at 37.312 kb on - strand at 37.312 kb on - strand at 37.347 kb on + strand at 37.349 kb on + strand at 37.349 kb on + strand at 37.349 kb on + strand at 37.349 kb on + strand at 37.349 kb on + strand at 37.349 kb on + strand at 37.350 kb on - strand at 37.350 kb on - strand at 37.350 kb on - strand at 37.350 kb on - strand at 37.412 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 34,609 - GFF5181 0.34 +1.1 34,609 - GFF5181 0.34 +2.1 34,772 + -0.8 34,773 - +0.9 34,905 + +0.3 34,905 + -0.0 34,929 + -0.1 34,949 + +1.4 34,950 - +1.3 35,005 - -0.1 35,005 - -0.3 35,005 - -1.0 35,007 - -0.7 35,027 + +0.6 35,133 + -0.5 35,133 + +0.7 35,134 - +0.8 35,134 - +0.6 35,134 - -0.1 35,134 - -0.3 35,417 + +0.2 35,417 + -1.0 35,418 - -0.8 35,418 - -0.3 35,482 + -0.7 35,482 + -0.6 35,482 + -0.2 35,483 - +2.6 35,483 - -0.7 35,483 - -0.1 35,483 - -0.4 35,483 - +0.1 35,483 - -0.9 35,483 - -0.2 35,483 - +0.0 35,531 + GFF5182 0.11 -0.7 35,579 + GFF5182 0.15 +0.5 35,580 - GFF5182 0.16 -0.9 35,580 - GFF5182 0.16 +0.2 35,580 - GFF5182 0.16 +0.8 35,580 - GFF5182 0.16 -0.1 35,580 - GFF5182 0.16 -0.3 35,580 - GFF5182 0.16 -0.4 35,580 - GFF5182 0.16 -1.0 35,633 + GFF5182 0.21 +0.9 35,634 - GFF5182 0.21 -0.5 35,634 - GFF5182 0.21 -1.0 35,634 - GFF5182 0.21 +0.8 35,735 + GFF5182 0.30 -0.1 35,735 + GFF5182 0.30 -2.3 35,735 + GFF5182 0.30 +0.5 35,736 - GFF5182 0.30 +0.2 35,736 - GFF5182 0.30 +0.2 35,736 - GFF5182 0.30 +0.1 35,758 - GFF5182 0.32 -0.3 35,922 + GFF5182 0.48 -1.7 35,922 + GFF5182 0.48 +0.2 35,922 + GFF5182 0.48 +0.6 35,923 - GFF5182 0.48 -1.7 35,925 + GFF5182 0.48 +0.5 35,926 - GFF5182 0.48 -0.5 35,926 - GFF5182 0.48 +0.9 35,968 - GFF5182 0.52 -0.0 36,086 + GFF5182 0.64 -0.2 36,086 + GFF5182 0.64 -0.4 36,087 - GFF5182 0.64 +0.6 36,121 - GFF5182 0.67 -0.8 36,121 - GFF5182 0.67 -0.1 36,126 + GFF5182 0.67 -0.1 36,126 + GFF5182 0.67 -2.0 36,126 + GFF5182 0.67 +0.8 36,127 - GFF5182 0.68 -1.1 36,127 - GFF5182 0.68 -0.2 36,127 - GFF5182 0.68 +0.2 36,127 - GFF5182 0.68 -0.0 36,127 - GFF5182 0.68 +1.4 36,127 - GFF5182 0.68 -1.5 36,127 - GFF5182 0.68 -0.5 36,128 + GFF5182 0.68 +0.4 36,128 + GFF5182 0.68 +1.0 36,128 + GFF5182 0.68 -0.9 36,128 + GFF5182 0.68 -0.1 36,128 + GFF5182 0.68 -0.5 36,128 + GFF5182 0.68 +0.2 36,128 + GFF5182 0.68 -0.8 36,128 + GFF5182 0.68 -0.9 36,129 - GFF5182 0.68 +0.7 36,129 - GFF5182 0.68 -0.8 36,129 - GFF5182 0.68 +0.3 36,129 - GFF5182 0.68 -0.2 36,129 - GFF5182 0.68 +1.0 36,129 - GFF5182 0.68 -0.3 36,129 - GFF5182 0.68 +0.9 36,129 - GFF5182 0.68 +0.1 36,129 - GFF5182 0.68 -0.5 36,129 - GFF5182 0.68 +0.7 36,171 + GFF5182 0.72 +0.1 36,270 - GFF5182 0.81 +0.2 36,270 - GFF5182 0.81 -0.7 36,287 + GFF5182 0.83 +1.4 36,287 + GFF5182 0.83 +0.2 36,287 + GFF5182 0.83 -0.5 36,288 - GFF5182 0.83 +0.6 36,288 - GFF5182 0.83 -0.4 36,359 + GFF5182 0.90 +1.6 36,417 + -0.0 36,417 + +0.6 36,417 + +0.4 36,418 - +0.2 36,418 - -0.5 36,418 - +0.8 36,418 - -2.0 36,418 - +0.1 36,423 + +0.1 36,424 - +1.2 36,470 + -0.1 36,470 + -1.2 36,474 + -1.7 36,589 + GFF5183 0.89 -0.8 36,589 + GFF5183 0.89 -1.4 36,656 + -0.1 36,735 + -0.4 36,735 + -2.2 36,735 + +1.1 36,735 + -0.6 36,735 + -2.3 36,735 + -0.4 36,735 + -1.1 36,736 - +0.2 36,736 - +0.3 36,736 - +0.6 36,736 - +0.2 36,736 - -1.3 36,736 - -1.5 36,741 + -0.0 36,741 + -0.6 36,741 + +1.4 36,742 - +1.8 36,742 - +0.7 36,767 + -0.0 36,767 + -0.5 36,767 + +0.3 36,767 + -2.7 36,768 - +0.6 36,768 - -1.4 36,906 + +0.2 36,906 + -0.5 36,964 + +0.6 36,964 + -0.4 36,964 + +0.0 36,964 + -1.4 36,964 + +0.0 36,964 + -0.5 36,965 - +0.4 36,965 - -0.9 36,965 - -0.9 36,965 - -0.3 36,983 + -0.1 36,983 + -0.2 36,983 + +0.2 36,983 + +0.8 36,983 + +0.3 36,984 - +0.0 36,984 - -0.3 36,984 - +0.1 36,984 - +0.7 37,021 - -0.2 37,030 + -0.4 37,030 + -1.3 37,031 - -0.3 37,038 + +1.8 37,038 + -1.6 37,038 + +0.5 37,038 + -0.5 37,038 + -1.0 37,038 + +1.0 37,039 - -0.2 37,039 - -0.6 37,039 - +0.1 37,039 - -0.1 37,074 + GFF5184 0.12 -0.8 37,075 - GFF5184 0.12 +0.2 37,075 - GFF5184 0.12 -0.4 37,090 + GFF5184 0.17 -0.6 37,178 - GFF5184 0.46 -0.7 37,232 + GFF5184 0.64 -0.1 37,233 - GFF5184 0.64 +0.2 37,233 - GFF5184 0.64 -0.1 37,233 - GFF5184 0.64 -1.0 37,312 - +1.5 37,312 - -0.3 37,347 + -0.9 37,349 + -1.6 37,349 + -0.6 37,349 + -0.6 37,349 + -0.7 37,349 + +0.3 37,349 + +0.2 37,350 - -0.4 37,350 - -0.6 37,350 - +0.0 37,350 - -0.1 37,412 - +0.0
Or see this region's nucleotide sequence