Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5052 and GFF5053 overlap by 4 nucleotides GFF5053 and GFF5054 are separated by 59 nucleotides GFF5054 and GFF5055 overlap by 4 nucleotides GFF5055 and GFF5056 are separated by 22 nucleotides
GFF5052 - FAD-dependent oxidoreductase SPO0682, at 152,900 to 154,540
GFF5052
GFF5053 - Uncharacterized protein SPO0681, at 154,537 to 154,749
GFF5053
GFF5054 - Homogentisate 1,2-dioxygenase (EC 1.13.11.5), at 154,809 to 156,119
GFF5054
GFF5055 - BUG/TctC family periplasmic protein, at 156,116 to 157,090
GFF5055
GFF5056 - Fumarylacetoacetase (EC 3.7.1.2), at 157,113 to 158,378
GFF5056
Position (kb)
154
155
156
157 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 153.912 kb on + strand, within GFF5052 at 153.912 kb on + strand, within GFF5052 at 153.913 kb on - strand, within GFF5052 at 153.959 kb on + strand, within GFF5052 at 153.959 kb on + strand, within GFF5052 at 153.959 kb on + strand, within GFF5052 at 153.959 kb on + strand, within GFF5052 at 153.959 kb on + strand, within GFF5052 at 153.959 kb on + strand, within GFF5052 at 153.959 kb on + strand, within GFF5052 at 153.959 kb on + strand, within GFF5052 at 153.959 kb on + strand, within GFF5052 at 153.959 kb on + strand, within GFF5052 at 153.959 kb on + strand, within GFF5052 at 153.959 kb on + strand, within GFF5052 at 153.959 kb on + strand, within GFF5052 at 153.959 kb on + strand, within GFF5052 at 153.959 kb on + strand, within GFF5052 at 153.959 kb on + strand, within GFF5052 at 153.959 kb on + strand, within GFF5052 at 153.960 kb on - strand, within GFF5052 at 153.960 kb on - strand, within GFF5052 at 153.960 kb on - strand, within GFF5052 at 153.960 kb on - strand, within GFF5052 at 153.960 kb on - strand, within GFF5052 at 153.960 kb on - strand, within GFF5052 at 153.960 kb on - strand, within GFF5052 at 153.960 kb on - strand, within GFF5052 at 153.960 kb on - strand, within GFF5052 at 153.960 kb on - strand, within GFF5052 at 153.960 kb on - strand, within GFF5052 at 153.960 kb on - strand, within GFF5052 at 153.960 kb on - strand, within GFF5052 at 153.960 kb on - strand, within GFF5052 at 153.960 kb on - strand, within GFF5052 at 153.960 kb on - strand, within GFF5052 at 153.960 kb on - strand, within GFF5052 at 153.960 kb on - strand, within GFF5052 at 154.419 kb on - strand at 154.442 kb on + strand at 154.442 kb on + strand at 154.442 kb on + strand at 154.443 kb on - strand at 154.443 kb on - strand at 154.606 kb on + strand, within GFF5053 at 154.606 kb on + strand, within GFF5053 at 154.607 kb on - strand, within GFF5053 at 154.802 kb on - strand at 154.842 kb on + strand at 154.843 kb on - strand at 154.843 kb on - strand at 154.866 kb on + strand at 154.866 kb on + strand at 154.866 kb on + strand at 154.866 kb on + strand at 154.866 kb on + strand at 154.867 kb on - strand at 154.867 kb on - strand at 154.867 kb on - strand at 154.938 kb on + strand at 154.938 kb on + strand at 154.938 kb on + strand at 154.939 kb on - strand at 154.939 kb on - strand at 154.939 kb on - strand at 154.939 kb on - strand at 154.939 kb on - strand at 155.004 kb on + strand, within GFF5054 at 155.037 kb on + strand, within GFF5054 at 155.038 kb on - strand, within GFF5054 at 155.046 kb on + strand, within GFF5054 at 155.046 kb on + strand, within GFF5054 at 155.046 kb on + strand, within GFF5054 at 155.046 kb on + strand, within GFF5054 at 155.047 kb on - strand, within GFF5054 at 155.047 kb on - strand, within GFF5054 at 155.047 kb on - strand, within GFF5054 at 155.047 kb on - strand, within GFF5054 at 155.047 kb on - strand, within GFF5054 at 155.223 kb on + strand, within GFF5054 at 155.223 kb on + strand, within GFF5054 at 155.223 kb on + strand, within GFF5054 at 155.223 kb on + strand, within GFF5054 at 155.223 kb on + strand, within GFF5054 at 155.223 kb on + strand, within GFF5054 at 155.224 kb on - strand, within GFF5054 at 155.224 kb on - strand, within GFF5054 at 155.224 kb on - strand, within GFF5054 at 155.224 kb on - strand, within GFF5054 at 155.224 kb on - strand, within GFF5054 at 155.224 kb on - strand, within GFF5054 at 155.224 kb on - strand, within GFF5054 at 155.415 kb on + strand, within GFF5054 at 155.415 kb on + strand, within GFF5054 at 155.416 kb on - strand, within GFF5054 at 155.430 kb on + strand, within GFF5054 at 155.431 kb on - strand, within GFF5054 at 155.431 kb on - strand, within GFF5054 at 155.431 kb on - strand, within GFF5054 at 155.431 kb on - strand, within GFF5054 at 155.541 kb on + strand, within GFF5054 at 155.542 kb on - strand, within GFF5054 at 155.644 kb on - strand, within GFF5054 at 155.841 kb on + strand, within GFF5054 at 155.862 kb on + strand, within GFF5054 at 155.863 kb on - strand, within GFF5054 at 155.863 kb on - strand, within GFF5054 at 155.863 kb on - strand, within GFF5054 at 155.863 kb on - strand, within GFF5054 at 156.075 kb on + strand at 156.075 kb on + strand at 156.076 kb on - strand at 156.198 kb on - strand at 156.198 kb on - strand at 156.230 kb on + strand, within GFF5055 at 156.230 kb on + strand, within GFF5055 at 156.230 kb on + strand, within GFF5055 at 156.230 kb on + strand, within GFF5055 at 156.230 kb on + strand, within GFF5055 at 156.230 kb on + strand, within GFF5055 at 156.231 kb on - strand, within GFF5055 at 156.231 kb on - strand, within GFF5055 at 156.231 kb on - strand, within GFF5055 at 156.231 kb on - strand, within GFF5055 at 156.380 kb on + strand, within GFF5055 at 156.380 kb on + strand, within GFF5055 at 156.380 kb on + strand, within GFF5055 at 156.381 kb on - strand, within GFF5055 at 156.381 kb on - strand, within GFF5055 at 156.381 kb on - strand, within GFF5055 at 156.381 kb on - strand, within GFF5055 at 156.381 kb on - strand, within GFF5055 at 156.381 kb on - strand, within GFF5055 at 156.431 kb on + strand, within GFF5055 at 156.431 kb on + strand, within GFF5055 at 156.431 kb on + strand, within GFF5055 at 156.431 kb on + strand, within GFF5055 at 156.431 kb on + strand, within GFF5055 at 156.431 kb on + strand, within GFF5055 at 156.431 kb on + strand, within GFF5055 at 156.431 kb on + strand, within GFF5055 at 156.432 kb on - strand, within GFF5055 at 156.432 kb on - strand, within GFF5055 at 156.432 kb on - strand, within GFF5055 at 156.432 kb on - strand, within GFF5055 at 156.432 kb on - strand, within GFF5055 at 156.432 kb on - strand, within GFF5055 at 156.432 kb on - strand, within GFF5055 at 156.432 kb on - strand, within GFF5055 at 156.581 kb on + strand, within GFF5055 at 156.581 kb on + strand, within GFF5055 at 156.582 kb on - strand, within GFF5055 at 156.668 kb on + strand, within GFF5055 at 156.668 kb on + strand, within GFF5055 at 156.668 kb on + strand, within GFF5055 at 156.668 kb on + strand, within GFF5055 at 156.668 kb on + strand, within GFF5055 at 156.668 kb on + strand, within GFF5055 at 156.668 kb on + strand, within GFF5055 at 156.668 kb on + strand, within GFF5055 at 156.669 kb on - strand, within GFF5055 at 156.669 kb on - strand, within GFF5055 at 156.669 kb on - strand, within GFF5055 at 156.669 kb on - strand, within GFF5055 at 156.669 kb on - strand, within GFF5055 at 156.755 kb on - strand, within GFF5055 at 156.881 kb on + strand, within GFF5055 at 156.881 kb on + strand, within GFF5055 at 156.881 kb on + strand, within GFF5055 at 156.882 kb on - strand, within GFF5055 at 156.882 kb on - strand, within GFF5055 at 156.882 kb on - strand, within GFF5055 at 156.882 kb on - strand, within GFF5055 at 157.040 kb on + strand at 157.040 kb on + strand at 157.040 kb on + strand at 157.041 kb on - strand at 157.041 kb on - strand at 157.108 kb on + strand at 157.108 kb on + strand at 157.108 kb on + strand at 157.108 kb on + strand at 157.108 kb on + strand at 157.108 kb on + strand at 157.108 kb on + strand at 157.109 kb on - strand at 157.109 kb on - strand at 157.109 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 153,912 + GFF5052 0.62 +0.1 153,912 + GFF5052 0.62 +0.3 153,913 - GFF5052 0.62 +1.3 153,959 + GFF5052 0.65 +1.4 153,959 + GFF5052 0.65 -0.1 153,959 + GFF5052 0.65 -0.7 153,959 + GFF5052 0.65 +2.8 153,959 + GFF5052 0.65 +0.0 153,959 + GFF5052 0.65 +0.6 153,959 + GFF5052 0.65 -2.2 153,959 + GFF5052 0.65 +0.4 153,959 + GFF5052 0.65 +0.0 153,959 + GFF5052 0.65 -0.3 153,959 + GFF5052 0.65 -2.0 153,959 + GFF5052 0.65 +0.4 153,959 + GFF5052 0.65 -0.4 153,959 + GFF5052 0.65 -0.9 153,959 + GFF5052 0.65 -0.3 153,959 + GFF5052 0.65 -0.5 153,959 + GFF5052 0.65 -0.3 153,960 - GFF5052 0.65 +0.2 153,960 - GFF5052 0.65 +1.3 153,960 - GFF5052 0.65 -0.7 153,960 - GFF5052 0.65 -0.7 153,960 - GFF5052 0.65 +0.3 153,960 - GFF5052 0.65 -1.9 153,960 - GFF5052 0.65 -0.0 153,960 - GFF5052 0.65 -0.7 153,960 - GFF5052 0.65 -0.3 153,960 - GFF5052 0.65 -0.6 153,960 - GFF5052 0.65 +0.8 153,960 - GFF5052 0.65 +0.4 153,960 - GFF5052 0.65 -0.7 153,960 - GFF5052 0.65 +0.0 153,960 - GFF5052 0.65 +1.8 153,960 - GFF5052 0.65 +0.4 153,960 - GFF5052 0.65 -0.2 153,960 - GFF5052 0.65 -1.5 154,419 - +0.5 154,442 + +0.2 154,442 + -0.5 154,442 + +0.3 154,443 - -0.2 154,443 - -0.4 154,606 + GFF5053 0.32 -0.4 154,606 + GFF5053 0.32 -1.3 154,607 - GFF5053 0.33 +0.2 154,802 - -1.1 154,842 + -0.7 154,843 - -1.3 154,843 - -1.8 154,866 + -0.5 154,866 + +0.1 154,866 + +1.4 154,866 + +0.1 154,866 + -0.7 154,867 - +2.3 154,867 - +0.2 154,867 - +1.4 154,938 + -1.1 154,938 + -0.6 154,938 + -0.4 154,939 - -0.9 154,939 - -0.5 154,939 - +0.0 154,939 - -0.6 154,939 - +0.5 155,004 + GFF5054 0.15 +0.0 155,037 + GFF5054 0.17 -0.2 155,038 - GFF5054 0.17 +1.4 155,046 + GFF5054 0.18 +0.1 155,046 + GFF5054 0.18 +0.1 155,046 + GFF5054 0.18 +0.3 155,046 + GFF5054 0.18 -0.0 155,047 - GFF5054 0.18 -1.1 155,047 - GFF5054 0.18 +1.2 155,047 - GFF5054 0.18 -0.1 155,047 - GFF5054 0.18 +0.0 155,047 - GFF5054 0.18 +0.3 155,223 + GFF5054 0.32 +1.3 155,223 + GFF5054 0.32 +0.6 155,223 + GFF5054 0.32 -0.3 155,223 + GFF5054 0.32 +0.4 155,223 + GFF5054 0.32 +1.2 155,223 + GFF5054 0.32 -0.9 155,224 - GFF5054 0.32 +1.8 155,224 - GFF5054 0.32 -0.0 155,224 - GFF5054 0.32 -0.8 155,224 - GFF5054 0.32 -0.6 155,224 - GFF5054 0.32 +0.8 155,224 - GFF5054 0.32 -1.0 155,224 - GFF5054 0.32 +0.1 155,415 + GFF5054 0.46 -0.6 155,415 + GFF5054 0.46 -1.3 155,416 - GFF5054 0.46 -0.2 155,430 + GFF5054 0.47 +1.2 155,431 - GFF5054 0.47 -0.4 155,431 - GFF5054 0.47 -1.1 155,431 - GFF5054 0.47 +0.5 155,431 - GFF5054 0.47 +1.3 155,541 + GFF5054 0.56 -0.4 155,542 - GFF5054 0.56 +0.8 155,644 - GFF5054 0.64 -0.4 155,841 + GFF5054 0.79 +0.3 155,862 + GFF5054 0.80 +0.1 155,863 - GFF5054 0.80 -0.3 155,863 - GFF5054 0.80 -0.5 155,863 - GFF5054 0.80 +0.7 155,863 - GFF5054 0.80 +1.4 156,075 + -1.2 156,075 + -0.4 156,076 - +0.3 156,198 - -0.9 156,198 - +0.3 156,230 + GFF5055 0.12 -0.3 156,230 + GFF5055 0.12 +0.4 156,230 + GFF5055 0.12 +0.1 156,230 + GFF5055 0.12 +0.5 156,230 + GFF5055 0.12 -0.1 156,230 + GFF5055 0.12 -0.1 156,231 - GFF5055 0.12 -0.3 156,231 - GFF5055 0.12 +0.0 156,231 - GFF5055 0.12 -1.0 156,231 - GFF5055 0.12 -1.5 156,380 + GFF5055 0.27 -0.4 156,380 + GFF5055 0.27 +0.2 156,380 + GFF5055 0.27 +0.0 156,381 - GFF5055 0.27 +0.3 156,381 - GFF5055 0.27 +0.3 156,381 - GFF5055 0.27 -0.7 156,381 - GFF5055 0.27 +0.8 156,381 - GFF5055 0.27 +0.7 156,381 - GFF5055 0.27 -1.1 156,431 + GFF5055 0.32 +0.6 156,431 + GFF5055 0.32 -0.7 156,431 + GFF5055 0.32 +0.4 156,431 + GFF5055 0.32 +1.3 156,431 + GFF5055 0.32 +0.7 156,431 + GFF5055 0.32 -0.6 156,431 + GFF5055 0.32 +1.5 156,431 + GFF5055 0.32 -0.2 156,432 - GFF5055 0.32 +0.3 156,432 - GFF5055 0.32 +0.3 156,432 - GFF5055 0.32 -1.2 156,432 - GFF5055 0.32 -0.2 156,432 - GFF5055 0.32 -0.1 156,432 - GFF5055 0.32 -0.5 156,432 - GFF5055 0.32 -0.2 156,432 - GFF5055 0.32 -0.8 156,581 + GFF5055 0.48 -0.2 156,581 + GFF5055 0.48 +0.0 156,582 - GFF5055 0.48 -1.3 156,668 + GFF5055 0.57 -0.6 156,668 + GFF5055 0.57 +1.1 156,668 + GFF5055 0.57 -0.8 156,668 + GFF5055 0.57 +1.4 156,668 + GFF5055 0.57 +0.5 156,668 + GFF5055 0.57 +0.7 156,668 + GFF5055 0.57 +1.8 156,668 + GFF5055 0.57 +0.5 156,669 - GFF5055 0.57 -0.2 156,669 - GFF5055 0.57 +0.5 156,669 - GFF5055 0.57 +0.8 156,669 - GFF5055 0.57 +0.5 156,669 - GFF5055 0.57 +1.3 156,755 - GFF5055 0.66 -0.4 156,881 + GFF5055 0.78 -0.8 156,881 + GFF5055 0.78 -0.5 156,881 + GFF5055 0.78 -0.7 156,882 - GFF5055 0.79 -1.7 156,882 - GFF5055 0.79 +0.4 156,882 - GFF5055 0.79 +0.3 156,882 - GFF5055 0.79 -1.0 157,040 + -0.2 157,040 + +0.5 157,040 + -1.2 157,041 - -0.4 157,041 - +0.0 157,108 + -0.3 157,108 + +0.3 157,108 + -2.7 157,108 + -0.2 157,108 + -0.1 157,108 + -1.6 157,108 + -0.5 157,109 - -1.0 157,109 - -0.2 157,109 - +0.1
Or see this region's nucleotide sequence