Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4322 and GFF4323 are separated by 14 nucleotides GFF4323 and GFF4324 are separated by 26 nucleotides GFF4324 and GFF4325 overlap by 4 nucleotides GFF4325 and GFF4326 are separated by 82 nucleotides
GFF4322 - Acyl-CoA dehydrogenase, at 244,422 to 245,582
GFF4322
GFF4323 - no description, at 245,597 to 246,220
GFF4323
GFF4324 - no description, at 246,247 to 247,281
GFF4324
GFF4325 - hypothetical protein, at 247,278 to 247,781
GFF4325
GFF4326 - Branched-chain amino acid ABC transporter, substrate-binding protein LivJ (TC 3.A.1.4.1), at 247,864 to 249,090
GFF4326
Position (kb) 246
247
248 Strain fitness (log2 ratio) -1
0
1 at 245.565 kb on + strand at 245.565 kb on + strand at 246.650 kb on - strand, within GFF4324 at 247.015 kb on + strand, within GFF4324 at 247.015 kb on + strand, within GFF4324 at 247.016 kb on - strand, within GFF4324 at 247.066 kb on + strand, within GFF4324 at 247.066 kb on + strand, within GFF4324 at 248.023 kb on + strand, within GFF4326 at 248.024 kb on - strand, within GFF4326
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 245,565 + -0.5 245,565 + -0.8 246,650 - GFF4324 0.39 -0.5 247,015 + GFF4324 0.74 +0.5 247,015 + GFF4324 0.74 -0.0 247,016 - GFF4324 0.74 +0.9 247,066 + GFF4324 0.79 -0.3 247,066 + GFF4324 0.79 +0.4 248,023 + GFF4326 0.13 -0.1 248,024 - GFF4326 0.13 +1.1
Or see this region's nucleotide sequence