Strain Fitness in Variovorax sp. SCN45 around GFF4302

Experiment: M9 with Supernatant from Cyptococcus

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4301 and GFF4302 are separated by 42 nucleotidesGFF4302 and GFF4303 are separated by 145 nucleotidesGFF4303 and GFF4304 are separated by 149 nucleotides GFF4301 - Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases), at 220,831 to 221,745 GFF4301 GFF4302 - hypothetical protein AF1210, at 221,788 to 222,720 GFF4302 GFF4303 - Transcriptional regulator, MarR family, at 222,866 to 223,330 GFF4303 GFF4304 - Cyclohexanecarboxylate--CoA ligase, at 223,480 to 225,141 GFF4304 Position (kb) 221 222 223Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 221.270 kb on - strand, within GFF4301at 221.594 kb on - strand, within GFF4301at 222.059 kb on - strand, within GFF4302at 222.238 kb on + strand, within GFF4302at 222.305 kb on - strand, within GFF4302at 222.322 kb on + strand, within GFF4302at 222.322 kb on + strand, within GFF4302at 222.810 kb on - strandat 222.817 kb on - strandat 222.817 kb on - strandat 222.817 kb on - strandat 223.307 kb on + strandat 223.537 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus
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221,270 - GFF4301 0.48 +0.6
221,594 - GFF4301 0.83 -0.7
222,059 - GFF4302 0.29 -0.8
222,238 + GFF4302 0.48 +1.8
222,305 - GFF4302 0.55 -3.5
222,322 + GFF4302 0.57 -0.3
222,322 + GFF4302 0.57 -0.2
222,810 - +1.1
222,817 - -1.3
222,817 - -0.7
222,817 - -0.9
223,307 + -3.2
223,537 + -1.4

Or see this region's nucleotide sequence