Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2 and GFF3 are separated by 63 nucleotides GFF3 and GFF4 are separated by 7 nucleotides GFF4 and GFF5 overlap by 4 nucleotides
GFF2 - Transcription accessory protein (S1 RNA-binding domain), at 1,164 to 3,539
GFF2
GFF3 - VapB protein (antitoxin to VapC), at 3,603 to 3,860
GFF3
GFF4 - VapC toxin protein, at 3,868 to 4,281
GFF4
GFF5 - FIG00537881: hypothetical protein, at 4,278 to 5,351
GFF5
Position (kb) 3
4
5 Strain fitness (log2 ratio) -2
-1
0
1
2 at 2.940 kb on + strand, within GFF2 at 2.940 kb on + strand, within GFF2 at 2.940 kb on + strand, within GFF2 at 3.537 kb on + strand at 3.537 kb on + strand at 3.537 kb on + strand at 3.537 kb on + strand at 3.537 kb on + strand at 3.537 kb on + strand at 3.537 kb on + strand at 3.537 kb on + strand at 3.537 kb on + strand at 3.537 kb on + strand at 3.538 kb on - strand at 3.538 kb on - strand at 3.538 kb on - strand at 3.538 kb on - strand at 3.538 kb on - strand at 3.538 kb on - strand at 3.538 kb on - strand at 3.538 kb on - strand at 3.538 kb on - strand at 3.538 kb on - strand at 3.538 kb on - strand at 3.538 kb on - strand at 3.541 kb on + strand at 3.541 kb on + strand at 3.541 kb on + strand at 3.541 kb on + strand at 3.542 kb on - strand at 3.542 kb on - strand at 3.542 kb on - strand at 3.542 kb on - strand at 3.571 kb on + strand at 3.572 kb on - strand at 3.871 kb on + strand at 3.871 kb on + strand at 3.871 kb on + strand at 3.871 kb on + strand at 3.871 kb on + strand at 3.872 kb on - strand at 3.872 kb on - strand at 3.872 kb on - strand at 3.872 kb on - strand at 3.872 kb on - strand at 3.872 kb on - strand at 4.081 kb on + strand, within GFF4 at 4.081 kb on + strand, within GFF4 at 4.081 kb on + strand, within GFF4 at 4.081 kb on + strand, within GFF4 at 4.081 kb on + strand, within GFF4 at 4.081 kb on + strand, within GFF4 at 4.082 kb on - strand, within GFF4 at 4.082 kb on - strand, within GFF4 at 4.082 kb on - strand, within GFF4 at 4.082 kb on - strand, within GFF4 at 4.082 kb on - strand, within GFF4 at 4.156 kb on + strand, within GFF4 at 4.156 kb on + strand, within GFF4 at 4.157 kb on - strand, within GFF4 at 4.157 kb on - strand, within GFF4 at 4.157 kb on - strand, within GFF4 at 4.157 kb on - strand, within GFF4 at 4.157 kb on - strand, within GFF4 at 4.250 kb on + strand at 4.250 kb on + strand at 4.250 kb on + strand at 4.250 kb on + strand at 4.250 kb on + strand at 4.250 kb on + strand at 4.250 kb on + strand at 4.250 kb on + strand at 4.250 kb on + strand at 4.250 kb on + strand at 4.251 kb on - strand at 4.251 kb on - strand at 4.251 kb on - strand at 4.251 kb on - strand at 4.251 kb on - strand at 4.251 kb on - strand at 4.296 kb on + strand at 4.296 kb on + strand at 4.296 kb on + strand at 4.296 kb on + strand at 4.297 kb on - strand at 4.297 kb on - strand at 4.297 kb on - strand at 4.297 kb on - strand at 4.548 kb on + strand, within GFF5 at 4.548 kb on + strand, within GFF5 at 4.549 kb on - strand, within GFF5 at 4.549 kb on - strand, within GFF5 at 4.641 kb on + strand, within GFF5 at 4.755 kb on + strand, within GFF5 at 4.755 kb on + strand, within GFF5 at 4.755 kb on + strand, within GFF5 at 4.756 kb on - strand, within GFF5 at 4.756 kb on - strand, within GFF5 at 4.756 kb on - strand, within GFF5 at 4.986 kb on + strand, within GFF5 at 5.010 kb on + strand, within GFF5 at 5.010 kb on + strand, within GFF5 at 5.011 kb on - strand, within GFF5 at 5.011 kb on - strand, within GFF5 at 5.028 kb on + strand, within GFF5 at 5.058 kb on + strand, within GFF5
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 2,940 + GFF2 0.75 -0.2 2,940 + GFF2 0.75 -0.3 2,940 + GFF2 0.75 -0.3 3,537 + +0.8 3,537 + -0.2 3,537 + +1.4 3,537 + -0.2 3,537 + +0.7 3,537 + -0.6 3,537 + +0.1 3,537 + +0.4 3,537 + -0.2 3,537 + +0.4 3,538 - -1.4 3,538 - -0.1 3,538 - +0.1 3,538 - -0.8 3,538 - +0.0 3,538 - -0.7 3,538 - -0.1 3,538 - +0.1 3,538 - +1.0 3,538 - -0.1 3,538 - -1.2 3,538 - -0.6 3,541 + +0.4 3,541 + +0.2 3,541 + -0.4 3,541 + -0.0 3,542 - +0.2 3,542 - -0.2 3,542 - +0.0 3,542 - -2.3 3,571 + -0.5 3,572 - +0.2 3,871 + +0.3 3,871 + +0.8 3,871 + +0.3 3,871 + -0.7 3,871 + -1.1 3,872 - +0.4 3,872 - -0.1 3,872 - +0.2 3,872 - -0.0 3,872 - -0.6 3,872 - -1.8 4,081 + GFF4 0.51 -1.2 4,081 + GFF4 0.51 -0.2 4,081 + GFF4 0.51 -1.8 4,081 + GFF4 0.51 -0.2 4,081 + GFF4 0.51 -1.2 4,081 + GFF4 0.51 -0.4 4,082 - GFF4 0.52 +0.4 4,082 - GFF4 0.52 -0.6 4,082 - GFF4 0.52 -0.4 4,082 - GFF4 0.52 -0.2 4,082 - GFF4 0.52 +1.4 4,156 + GFF4 0.70 +0.1 4,156 + GFF4 0.70 -0.1 4,157 - GFF4 0.70 +0.8 4,157 - GFF4 0.70 -0.9 4,157 - GFF4 0.70 -1.4 4,157 - GFF4 0.70 -1.2 4,157 - GFF4 0.70 -0.8 4,250 + +0.6 4,250 + -0.4 4,250 + -1.9 4,250 + +0.3 4,250 + +0.2 4,250 + -1.0 4,250 + -0.4 4,250 + +0.7 4,250 + -0.2 4,250 + +0.5 4,251 - -0.2 4,251 - -1.3 4,251 - -0.0 4,251 - +0.1 4,251 - -0.6 4,251 - -0.3 4,296 + -0.3 4,296 + +0.8 4,296 + -0.4 4,296 + -0.3 4,297 - -1.0 4,297 - +0.1 4,297 - +0.2 4,297 - -2.3 4,548 + GFF5 0.25 -0.3 4,548 + GFF5 0.25 -0.5 4,549 - GFF5 0.25 -2.7 4,549 - GFF5 0.25 -0.6 4,641 + GFF5 0.34 -0.0 4,755 + GFF5 0.44 -0.8 4,755 + GFF5 0.44 -0.1 4,755 + GFF5 0.44 -0.6 4,756 - GFF5 0.45 +0.3 4,756 - GFF5 0.45 -0.4 4,756 - GFF5 0.45 -0.6 4,986 + GFF5 0.66 +0.2 5,010 + GFF5 0.68 +2.0 5,010 + GFF5 0.68 -0.7 5,011 - GFF5 0.68 -0.3 5,011 - GFF5 0.68 -1.4 5,028 + GFF5 0.70 +0.6 5,058 + GFF5 0.73 -0.6
Or see this region's nucleotide sequence