Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3986 and GFF3987 are separated by 53 nucleotides GFF3987 and GFF3988 overlap by 4 nucleotides GFF3988 and GFF3989 are separated by 2 nucleotides GFF3989 and GFF3990 are separated by 6 nucleotides
GFF3986 - Oxidoreductase, short-chain dehydrogenase/reductase family, at 190,701 to 191,447
GFF3986
GFF3987 - 3-hydroxybutyrate dehydrogenase (EC 1.1.1.30), at 191,501 to 192,277
GFF3987
GFF3988 - Transcriptional regulator, MarR family, at 192,274 to 192,804
GFF3988
GFF3989 - PUTATIVE 4-HYDROXYBENZOYL-COA THIOESTERASE PROTEIN( EC:3.1.2.23 ), at 192,807 to 193,241
GFF3989
GFF3990 - hypothetical protein, at 193,248 to 193,643
GFF3990
Position (kb) 192
193 Strain fitness (log2 ratio) -2
-1
0
1 at 191.373 kb on + strand at 191.504 kb on + strand at 191.505 kb on - strand at 191.709 kb on + strand, within GFF3987 at 191.709 kb on + strand, within GFF3987 at 191.710 kb on - strand, within GFF3987 at 191.917 kb on + strand, within GFF3987 at 191.946 kb on - strand, within GFF3987 at 191.946 kb on - strand, within GFF3987 at 191.946 kb on - strand, within GFF3987 at 191.946 kb on - strand, within GFF3987 at 191.946 kb on - strand, within GFF3987 at 191.951 kb on + strand, within GFF3987 at 191.951 kb on + strand, within GFF3987 at 191.952 kb on - strand, within GFF3987 at 191.952 kb on - strand, within GFF3987 at 191.952 kb on - strand, within GFF3987 at 191.952 kb on - strand, within GFF3987 at 191.952 kb on - strand, within GFF3987 at 191.952 kb on - strand, within GFF3987 at 191.963 kb on + strand, within GFF3987 at 191.964 kb on - strand, within GFF3987 at 192.059 kb on + strand, within GFF3987 at 192.061 kb on + strand, within GFF3987 at 192.062 kb on - strand, within GFF3987 at 192.393 kb on + strand, within GFF3988 at 192.445 kb on - strand, within GFF3988 at 192.466 kb on + strand, within GFF3988 at 192.467 kb on - strand, within GFF3988 at 192.467 kb on - strand, within GFF3988 at 192.487 kb on + strand, within GFF3988 at 192.523 kb on + strand, within GFF3988 at 192.523 kb on + strand, within GFF3988 at 192.524 kb on - strand, within GFF3988 at 192.524 kb on - strand, within GFF3988 at 192.524 kb on - strand, within GFF3988 at 192.741 kb on + strand, within GFF3988 at 192.883 kb on - strand, within GFF3989 at 192.892 kb on - strand, within GFF3989 at 192.892 kb on - strand, within GFF3989 at 192.951 kb on + strand, within GFF3989 at 192.951 kb on + strand, within GFF3989 at 192.952 kb on - strand, within GFF3989 at 193.274 kb on + strand at 193.515 kb on + strand, within GFF3990 at 193.515 kb on + strand, within GFF3990 at 193.516 kb on - strand, within GFF3990 at 193.516 kb on - strand, within GFF3990 at 193.516 kb on - strand, within GFF3990 at 193.516 kb on - strand, within GFF3990 at 193.634 kb on + strand at 193.635 kb on - strand at 193.759 kb on - strand at 193.759 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 191,373 + +0.0 191,504 + -0.9 191,505 - -0.6 191,709 + GFF3987 0.27 +0.8 191,709 + GFF3987 0.27 -1.0 191,710 - GFF3987 0.27 -0.1 191,917 + GFF3987 0.54 +1.7 191,946 - GFF3987 0.57 +0.5 191,946 - GFF3987 0.57 +0.2 191,946 - GFF3987 0.57 -0.4 191,946 - GFF3987 0.57 +0.3 191,946 - GFF3987 0.57 -1.0 191,951 + GFF3987 0.58 -0.5 191,951 + GFF3987 0.58 -0.3 191,952 - GFF3987 0.58 -0.7 191,952 - GFF3987 0.58 +0.7 191,952 - GFF3987 0.58 +0.3 191,952 - GFF3987 0.58 +0.1 191,952 - GFF3987 0.58 +0.4 191,952 - GFF3987 0.58 +1.2 191,963 + GFF3987 0.59 -0.7 191,964 - GFF3987 0.60 -2.1 192,059 + GFF3987 0.72 +0.5 192,061 + GFF3987 0.72 -0.9 192,062 - GFF3987 0.72 -0.3 192,393 + GFF3988 0.22 +0.1 192,445 - GFF3988 0.32 +0.6 192,466 + GFF3988 0.36 -1.3 192,467 - GFF3988 0.36 +0.4 192,467 - GFF3988 0.36 -0.1 192,487 + GFF3988 0.40 +0.5 192,523 + GFF3988 0.47 -0.7 192,523 + GFF3988 0.47 +0.2 192,524 - GFF3988 0.47 -1.1 192,524 - GFF3988 0.47 -0.6 192,524 - GFF3988 0.47 -0.0 192,741 + GFF3988 0.88 -0.2 192,883 - GFF3989 0.17 -1.9 192,892 - GFF3989 0.20 +0.6 192,892 - GFF3989 0.20 +0.8 192,951 + GFF3989 0.33 -0.8 192,951 + GFF3989 0.33 +0.3 192,952 - GFF3989 0.33 +0.2 193,274 + -0.7 193,515 + GFF3990 0.67 +0.1 193,515 + GFF3990 0.67 +0.3 193,516 - GFF3990 0.68 -0.6 193,516 - GFF3990 0.68 -0.0 193,516 - GFF3990 0.68 -0.8 193,516 - GFF3990 0.68 +0.2 193,634 + -0.6 193,635 - -1.0 193,759 - -1.2 193,759 - +0.1
Or see this region's nucleotide sequence