Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3736 and GFF3737 are separated by 85 nucleotides GFF3737 and GFF3738 overlap by 4 nucleotides GFF3738 and GFF3739 are separated by 29 nucleotides
GFF3736 - UPF0102 protein YraN, at 233,123 to 233,491
GFF3736
GFF3737 - DnaA initiator-associating protein DiaA, at 233,577 to 234,221
GFF3737
GFF3738 - Uncharacterized protein YraP, at 234,218 to 235,030
GFF3738
GFF3739 - 2-hydroxy-3-oxopropionate reductase (EC 1.1.1.60), at 235,060 to 235,968
GFF3739
Position (kb) 234
235
236 Strain fitness (log2 ratio) -2
-1
0
1 at 233.223 kb on - strand, within GFF3736 at 233.393 kb on + strand, within GFF3736 at 233.393 kb on + strand, within GFF3736 at 233.394 kb on - strand, within GFF3736 at 233.564 kb on - strand at 233.564 kb on - strand at 233.564 kb on - strand at 233.628 kb on + strand at 233.629 kb on - strand at 233.629 kb on - strand at 233.629 kb on - strand at 233.629 kb on - strand at 233.629 kb on - strand at 234.194 kb on - strand at 234.194 kb on - strand at 234.455 kb on + strand, within GFF3738 at 234.455 kb on + strand, within GFF3738 at 234.455 kb on + strand, within GFF3738 at 234.455 kb on + strand, within GFF3738 at 234.455 kb on + strand, within GFF3738 at 234.455 kb on + strand, within GFF3738 at 234.455 kb on + strand, within GFF3738 at 234.455 kb on + strand, within GFF3738 at 234.455 kb on + strand, within GFF3738 at 234.455 kb on + strand, within GFF3738 at 234.455 kb on + strand, within GFF3738 at 234.455 kb on + strand, within GFF3738 at 234.456 kb on - strand, within GFF3738 at 234.456 kb on - strand, within GFF3738 at 234.456 kb on - strand, within GFF3738 at 234.456 kb on - strand, within GFF3738 at 234.456 kb on - strand, within GFF3738 at 234.456 kb on - strand, within GFF3738 at 234.456 kb on - strand, within GFF3738 at 234.456 kb on - strand, within GFF3738 at 234.456 kb on - strand, within GFF3738 at 234.456 kb on - strand, within GFF3738 at 234.456 kb on - strand, within GFF3738 at 234.456 kb on - strand, within GFF3738 at 234.473 kb on + strand, within GFF3738 at 234.473 kb on + strand, within GFF3738 at 234.473 kb on + strand, within GFF3738 at 234.473 kb on + strand, within GFF3738 at 234.474 kb on - strand, within GFF3738 at 234.474 kb on - strand, within GFF3738 at 234.474 kb on - strand, within GFF3738 at 234.474 kb on - strand, within GFF3738 at 234.629 kb on + strand, within GFF3738 at 234.629 kb on + strand, within GFF3738 at 234.629 kb on + strand, within GFF3738 at 234.629 kb on + strand, within GFF3738 at 234.629 kb on + strand, within GFF3738 at 234.629 kb on + strand, within GFF3738 at 234.629 kb on + strand, within GFF3738 at 234.629 kb on + strand, within GFF3738 at 234.629 kb on + strand, within GFF3738 at 234.629 kb on + strand, within GFF3738 at 234.629 kb on + strand, within GFF3738 at 234.629 kb on + strand, within GFF3738 at 234.630 kb on - strand, within GFF3738 at 234.630 kb on - strand, within GFF3738 at 234.630 kb on - strand, within GFF3738 at 234.630 kb on - strand, within GFF3738 at 234.630 kb on - strand, within GFF3738 at 234.630 kb on - strand, within GFF3738 at 234.630 kb on - strand, within GFF3738 at 234.630 kb on - strand, within GFF3738 at 234.630 kb on - strand, within GFF3738 at 234.630 kb on - strand, within GFF3738 at 234.630 kb on - strand, within GFF3738 at 234.630 kb on - strand, within GFF3738 at 234.630 kb on - strand, within GFF3738 at 234.630 kb on - strand, within GFF3738 at 234.630 kb on - strand, within GFF3738 at 234.630 kb on - strand, within GFF3738 at 234.719 kb on + strand, within GFF3738 at 234.720 kb on - strand, within GFF3738 at 234.720 kb on - strand, within GFF3738 at 235.061 kb on + strand at 235.127 kb on + strand at 235.128 kb on - strand at 235.841 kb on + strand, within GFF3739 at 235.841 kb on + strand, within GFF3739 at 235.842 kb on - strand, within GFF3739 at 235.842 kb on - strand, within GFF3739 at 235.842 kb on - strand, within GFF3739 at 235.842 kb on - strand, within GFF3739 at 235.944 kb on - strand at 235.981 kb on + strand at 235.982 kb on - strand at 235.982 kb on - strand at 236.029 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 233,223 - GFF3736 0.27 +0.0 233,393 + GFF3736 0.73 +0.4 233,393 + GFF3736 0.73 -0.1 233,394 - GFF3736 0.73 +0.1 233,564 - -0.5 233,564 - +0.9 233,564 - -0.6 233,628 + -0.8 233,629 - -0.9 233,629 - +0.8 233,629 - -1.0 233,629 - -0.6 233,629 - -0.1 234,194 - +0.1 234,194 - +0.3 234,455 + GFF3738 0.29 -0.0 234,455 + GFF3738 0.29 -1.0 234,455 + GFF3738 0.29 -0.8 234,455 + GFF3738 0.29 -0.6 234,455 + GFF3738 0.29 +0.4 234,455 + GFF3738 0.29 -1.6 234,455 + GFF3738 0.29 -0.0 234,455 + GFF3738 0.29 -0.4 234,455 + GFF3738 0.29 -1.4 234,455 + GFF3738 0.29 +0.2 234,455 + GFF3738 0.29 -1.1 234,455 + GFF3738 0.29 +0.2 234,456 - GFF3738 0.29 -0.5 234,456 - GFF3738 0.29 -0.6 234,456 - GFF3738 0.29 -0.5 234,456 - GFF3738 0.29 -1.1 234,456 - GFF3738 0.29 -0.3 234,456 - GFF3738 0.29 -1.3 234,456 - GFF3738 0.29 -1.3 234,456 - GFF3738 0.29 -0.2 234,456 - GFF3738 0.29 -0.3 234,456 - GFF3738 0.29 -0.8 234,456 - GFF3738 0.29 +0.5 234,456 - GFF3738 0.29 -0.6 234,473 + GFF3738 0.31 +1.4 234,473 + GFF3738 0.31 -0.6 234,473 + GFF3738 0.31 +0.0 234,473 + GFF3738 0.31 -0.6 234,474 - GFF3738 0.31 +0.4 234,474 - GFF3738 0.31 -0.5 234,474 - GFF3738 0.31 -0.7 234,474 - GFF3738 0.31 -1.2 234,629 + GFF3738 0.51 +0.2 234,629 + GFF3738 0.51 +1.4 234,629 + GFF3738 0.51 +0.1 234,629 + GFF3738 0.51 +0.1 234,629 + GFF3738 0.51 +1.1 234,629 + GFF3738 0.51 -0.1 234,629 + GFF3738 0.51 -0.3 234,629 + GFF3738 0.51 -0.3 234,629 + GFF3738 0.51 -1.3 234,629 + GFF3738 0.51 -2.3 234,629 + GFF3738 0.51 -0.2 234,629 + GFF3738 0.51 +0.6 234,630 - GFF3738 0.51 -0.9 234,630 - GFF3738 0.51 -0.9 234,630 - GFF3738 0.51 -0.2 234,630 - GFF3738 0.51 -1.8 234,630 - GFF3738 0.51 -0.3 234,630 - GFF3738 0.51 -2.0 234,630 - GFF3738 0.51 +0.3 234,630 - GFF3738 0.51 -0.8 234,630 - GFF3738 0.51 -1.5 234,630 - GFF3738 0.51 -1.2 234,630 - GFF3738 0.51 +0.6 234,630 - GFF3738 0.51 -1.3 234,630 - GFF3738 0.51 +0.0 234,630 - GFF3738 0.51 +0.5 234,630 - GFF3738 0.51 -1.1 234,630 - GFF3738 0.51 +0.1 234,719 + GFF3738 0.62 -1.4 234,720 - GFF3738 0.62 -1.6 234,720 - GFF3738 0.62 -0.1 235,061 + +1.0 235,127 + +0.3 235,128 - -0.7 235,841 + GFF3739 0.86 -0.2 235,841 + GFF3739 0.86 -0.6 235,842 - GFF3739 0.86 -0.8 235,842 - GFF3739 0.86 -0.5 235,842 - GFF3739 0.86 -0.0 235,842 - GFF3739 0.86 -0.2 235,944 - -0.5 235,981 + -0.1 235,982 - -0.2 235,982 - -0.3 236,029 + +0.2
Or see this region's nucleotide sequence