Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3474 and GFF3475 overlap by 19 nucleotides GFF3475 and GFF3476 are separated by 85 nucleotides
GFF3474 - Alkyl sulfatase and related hydrolases, MBL-fold metallo-hydrolase superfamily, at 293,875 to 295,866
GFF3474
GFF3475 - Gate supefamily SpmA/SpmB domains protein, at 295,848 to 297,077
GFF3475
GFF3476 - Cardiolipin synthase (EC 2.7.8.-) phosphatidylethanolamine-utilizing, bacterial type ClsC, at 297,163 to 298,746
GFF3476
Position (kb)
295
296
297
298 Strain fitness (log2 ratio)
-2
-1
0
1 at 294.985 kb on + strand, within GFF3474 at 294.985 kb on + strand, within GFF3474 at 294.985 kb on + strand, within GFF3474 at 294.985 kb on + strand, within GFF3474 at 294.985 kb on + strand, within GFF3474 at 294.985 kb on + strand, within GFF3474 at 294.985 kb on + strand, within GFF3474 at 294.986 kb on - strand, within GFF3474 at 294.986 kb on - strand, within GFF3474 at 294.986 kb on - strand, within GFF3474 at 294.986 kb on - strand, within GFF3474 at 294.986 kb on - strand, within GFF3474 at 294.986 kb on - strand, within GFF3474 at 295.030 kb on + strand, within GFF3474 at 295.030 kb on + strand, within GFF3474 at 295.030 kb on + strand, within GFF3474 at 295.031 kb on - strand, within GFF3474 at 295.105 kb on + strand, within GFF3474 at 295.105 kb on + strand, within GFF3474 at 295.106 kb on - strand, within GFF3474 at 295.139 kb on - strand, within GFF3474 at 295.147 kb on + strand, within GFF3474 at 295.148 kb on - strand, within GFF3474 at 295.148 kb on - strand, within GFF3474 at 295.148 kb on - strand, within GFF3474 at 295.148 kb on - strand, within GFF3474 at 295.148 kb on - strand, within GFF3474 at 295.159 kb on + strand, within GFF3474 at 295.159 kb on + strand, within GFF3474 at 295.159 kb on + strand, within GFF3474 at 295.205 kb on - strand, within GFF3474 at 295.225 kb on + strand, within GFF3474 at 295.225 kb on + strand, within GFF3474 at 295.225 kb on + strand, within GFF3474 at 295.225 kb on + strand, within GFF3474 at 295.225 kb on + strand, within GFF3474 at 295.226 kb on - strand, within GFF3474 at 295.226 kb on - strand, within GFF3474 at 295.226 kb on - strand, within GFF3474 at 295.322 kb on - strand, within GFF3474 at 295.322 kb on - strand, within GFF3474 at 295.385 kb on - strand, within GFF3474 at 295.401 kb on + strand, within GFF3474 at 295.402 kb on - strand, within GFF3474 at 295.417 kb on + strand, within GFF3474 at 295.418 kb on - strand, within GFF3474 at 295.809 kb on + strand at 295.864 kb on + strand at 295.961 kb on - strand at 295.975 kb on + strand, within GFF3475 at 295.975 kb on + strand, within GFF3475 at 295.975 kb on + strand, within GFF3475 at 295.975 kb on + strand, within GFF3475 at 295.975 kb on + strand, within GFF3475 at 295.976 kb on - strand, within GFF3475 at 295.976 kb on - strand, within GFF3475 at 295.976 kb on - strand, within GFF3475 at 296.236 kb on + strand, within GFF3475 at 296.237 kb on - strand, within GFF3475 at 296.296 kb on + strand, within GFF3475 at 296.296 kb on + strand, within GFF3475 at 296.296 kb on + strand, within GFF3475 at 296.297 kb on - strand, within GFF3475 at 296.297 kb on - strand, within GFF3475 at 296.297 kb on - strand, within GFF3475 at 296.297 kb on - strand, within GFF3475 at 296.297 kb on - strand, within GFF3475 at 296.297 kb on - strand, within GFF3475 at 296.297 kb on - strand, within GFF3475 at 296.458 kb on + strand, within GFF3475 at 296.458 kb on + strand, within GFF3475 at 296.459 kb on - strand, within GFF3475 at 296.459 kb on - strand, within GFF3475 at 296.459 kb on - strand, within GFF3475 at 296.599 kb on + strand, within GFF3475 at 296.599 kb on + strand, within GFF3475 at 296.599 kb on + strand, within GFF3475 at 296.599 kb on + strand, within GFF3475 at 296.599 kb on + strand, within GFF3475 at 297.118 kb on + strand at 297.118 kb on + strand at 297.119 kb on - strand at 297.119 kb on - strand at 297.127 kb on - strand at 297.150 kb on + strand at 297.150 kb on + strand at 297.150 kb on + strand at 297.150 kb on + strand at 297.151 kb on - strand at 297.151 kb on - strand at 297.151 kb on - strand at 297.151 kb on - strand at 297.151 kb on - strand at 297.151 kb on - strand at 297.151 kb on - strand at 297.445 kb on + strand, within GFF3476 at 297.445 kb on + strand, within GFF3476 at 297.445 kb on + strand, within GFF3476 at 297.445 kb on + strand, within GFF3476 at 297.445 kb on + strand, within GFF3476 at 297.446 kb on - strand, within GFF3476 at 297.446 kb on - strand, within GFF3476 at 297.448 kb on + strand, within GFF3476 at 297.448 kb on + strand, within GFF3476 at 297.448 kb on + strand, within GFF3476 at 297.448 kb on + strand, within GFF3476 at 297.448 kb on + strand, within GFF3476 at 297.449 kb on - strand, within GFF3476 at 297.449 kb on - strand, within GFF3476 at 297.449 kb on - strand, within GFF3476 at 297.547 kb on + strand, within GFF3476 at 297.547 kb on + strand, within GFF3476 at 297.547 kb on + strand, within GFF3476 at 297.547 kb on + strand, within GFF3476 at 297.547 kb on + strand, within GFF3476 at 297.547 kb on + strand, within GFF3476 at 297.548 kb on - strand, within GFF3476 at 298.045 kb on + strand, within GFF3476 at 298.046 kb on - strand, within GFF3476
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 294,985 + GFF3474 0.56 -0.8 294,985 + GFF3474 0.56 +0.1 294,985 + GFF3474 0.56 +0.6 294,985 + GFF3474 0.56 -1.5 294,985 + GFF3474 0.56 -0.1 294,985 + GFF3474 0.56 +0.4 294,985 + GFF3474 0.56 -0.1 294,986 - GFF3474 0.56 -0.9 294,986 - GFF3474 0.56 -1.1 294,986 - GFF3474 0.56 -0.3 294,986 - GFF3474 0.56 -0.4 294,986 - GFF3474 0.56 +1.3 294,986 - GFF3474 0.56 -0.9 295,030 + GFF3474 0.58 +0.1 295,030 + GFF3474 0.58 +0.6 295,030 + GFF3474 0.58 -1.9 295,031 - GFF3474 0.58 +0.0 295,105 + GFF3474 0.62 -1.2 295,105 + GFF3474 0.62 -0.9 295,106 - GFF3474 0.62 +0.4 295,139 - GFF3474 0.63 +0.6 295,147 + GFF3474 0.64 +0.9 295,148 - GFF3474 0.64 -0.1 295,148 - GFF3474 0.64 -0.7 295,148 - GFF3474 0.64 +0.1 295,148 - GFF3474 0.64 +0.0 295,148 - GFF3474 0.64 -1.3 295,159 + GFF3474 0.64 +0.2 295,159 + GFF3474 0.64 -0.9 295,159 + GFF3474 0.64 -0.1 295,205 - GFF3474 0.67 +1.3 295,225 + GFF3474 0.68 +0.1 295,225 + GFF3474 0.68 -0.3 295,225 + GFF3474 0.68 -0.5 295,225 + GFF3474 0.68 +0.3 295,225 + GFF3474 0.68 -0.4 295,226 - GFF3474 0.68 -0.2 295,226 - GFF3474 0.68 -0.2 295,226 - GFF3474 0.68 -0.9 295,322 - GFF3474 0.73 -0.4 295,322 - GFF3474 0.73 -0.5 295,385 - GFF3474 0.76 +0.1 295,401 + GFF3474 0.77 -0.3 295,402 - GFF3474 0.77 -0.2 295,417 + GFF3474 0.77 +0.6 295,418 - GFF3474 0.77 -0.3 295,809 + -0.0 295,864 + -0.5 295,961 - +0.1 295,975 + GFF3475 0.10 -0.1 295,975 + GFF3475 0.10 -0.3 295,975 + GFF3475 0.10 +0.7 295,975 + GFF3475 0.10 -0.0 295,975 + GFF3475 0.10 +1.2 295,976 - GFF3475 0.10 -0.1 295,976 - GFF3475 0.10 -0.7 295,976 - GFF3475 0.10 +0.1 296,236 + GFF3475 0.32 +0.3 296,237 - GFF3475 0.32 -0.2 296,296 + GFF3475 0.36 +0.1 296,296 + GFF3475 0.36 +0.1 296,296 + GFF3475 0.36 -0.3 296,297 - GFF3475 0.37 -0.2 296,297 - GFF3475 0.37 -1.0 296,297 - GFF3475 0.37 +0.2 296,297 - GFF3475 0.37 -0.4 296,297 - GFF3475 0.37 -0.1 296,297 - GFF3475 0.37 -0.4 296,297 - GFF3475 0.37 +0.2 296,458 + GFF3475 0.50 -0.3 296,458 + GFF3475 0.50 -0.0 296,459 - GFF3475 0.50 +0.2 296,459 - GFF3475 0.50 -0.0 296,459 - GFF3475 0.50 -0.6 296,599 + GFF3475 0.61 +0.2 296,599 + GFF3475 0.61 -0.1 296,599 + GFF3475 0.61 +0.3 296,599 + GFF3475 0.61 -0.0 296,599 + GFF3475 0.61 +0.0 297,118 + -0.4 297,118 + +0.4 297,119 - -0.7 297,119 - -0.3 297,127 - +0.1 297,150 + -0.3 297,150 + -0.7 297,150 + -0.7 297,150 + -1.0 297,151 - +0.3 297,151 - +0.1 297,151 - -0.0 297,151 - -0.9 297,151 - -1.9 297,151 - +0.5 297,151 - -0.1 297,445 + GFF3476 0.18 -0.1 297,445 + GFF3476 0.18 -2.1 297,445 + GFF3476 0.18 -1.7 297,445 + GFF3476 0.18 -0.1 297,445 + GFF3476 0.18 -0.3 297,446 - GFF3476 0.18 -0.2 297,446 - GFF3476 0.18 -1.1 297,448 + GFF3476 0.18 -0.9 297,448 + GFF3476 0.18 -0.5 297,448 + GFF3476 0.18 +0.0 297,448 + GFF3476 0.18 -0.7 297,448 + GFF3476 0.18 -1.1 297,449 - GFF3476 0.18 +0.7 297,449 - GFF3476 0.18 -0.3 297,449 - GFF3476 0.18 +1.1 297,547 + GFF3476 0.24 -1.7 297,547 + GFF3476 0.24 +0.4 297,547 + GFF3476 0.24 -0.3 297,547 + GFF3476 0.24 +0.1 297,547 + GFF3476 0.24 +0.8 297,547 + GFF3476 0.24 -0.2 297,548 - GFF3476 0.24 +0.4 298,045 + GFF3476 0.56 +0.7 298,046 - GFF3476 0.56 -0.4
Or see this region's nucleotide sequence