Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3428 and GFF3429 are separated by 16 nucleotides GFF3429 and GFF3430 are separated by 7 nucleotides GFF3430 and GFF3431 are separated by 186 nucleotides
GFF3428 - 2-oxo-hepta-3-ene-1,7-dioic acid hydratase (EC 4.2.-.-), at 251,484 to 252,296
GFF3428
GFF3429 - Transcriptional regulator, AraC family, at 252,313 to 253,233
GFF3429
GFF3430 - Transcriptional regulator, LysR family, at 253,241 to 254,143
GFF3430
GFF3431 - Glutaryl-CoA dehydrogenase (EC 1.3.8.6), at 254,330 to 255,529
GFF3431
Position (kb) 253
254
255 Strain fitness (log2 ratio) -2
-1
0
1
2 at 252.294 kb on + strand at 252.305 kb on + strand at 252.305 kb on + strand at 252.731 kb on + strand, within GFF3429 at 252.731 kb on + strand, within GFF3429 at 252.732 kb on - strand, within GFF3429 at 252.740 kb on + strand, within GFF3429 at 252.741 kb on - strand, within GFF3429 at 253.058 kb on + strand, within GFF3429 at 253.058 kb on + strand, within GFF3429 at 253.109 kb on + strand, within GFF3429 at 253.109 kb on + strand, within GFF3429 at 253.110 kb on - strand, within GFF3429 at 253.110 kb on - strand, within GFF3429 at 253.326 kb on + strand at 253.326 kb on + strand at 253.326 kb on + strand at 253.326 kb on + strand at 253.327 kb on - strand at 253.327 kb on - strand at 253.327 kb on - strand at 253.329 kb on + strand at 253.329 kb on + strand at 253.329 kb on + strand at 253.329 kb on + strand at 253.329 kb on + strand at 253.329 kb on + strand at 253.329 kb on + strand at 253.329 kb on + strand at 253.330 kb on - strand at 253.330 kb on - strand at 253.330 kb on - strand at 253.330 kb on - strand at 253.330 kb on - strand at 253.470 kb on + strand, within GFF3430 at 253.470 kb on + strand, within GFF3430 at 253.471 kb on - strand, within GFF3430 at 253.704 kb on + strand, within GFF3430 at 253.704 kb on + strand, within GFF3430 at 253.705 kb on - strand, within GFF3430 at 253.705 kb on - strand, within GFF3430 at 253.938 kb on + strand, within GFF3430 at 253.938 kb on + strand, within GFF3430 at 253.938 kb on + strand, within GFF3430 at 253.938 kb on + strand, within GFF3430 at 253.938 kb on + strand, within GFF3430 at 254.076 kb on + strand at 254.076 kb on + strand at 254.076 kb on + strand at 254.076 kb on + strand at 254.076 kb on + strand at 254.077 kb on - strand at 254.077 kb on - strand at 254.077 kb on - strand at 254.077 kb on - strand at 254.077 kb on - strand at 254.145 kb on + strand at 254.145 kb on + strand at 254.145 kb on + strand at 254.145 kb on + strand at 254.145 kb on + strand at 254.145 kb on + strand at 254.145 kb on + strand at 254.145 kb on + strand at 254.145 kb on + strand at 254.145 kb on + strand at 254.145 kb on + strand at 254.146 kb on - strand at 254.146 kb on - strand at 254.146 kb on - strand at 254.146 kb on - strand at 254.146 kb on - strand at 254.158 kb on + strand at 254.218 kb on + strand at 254.219 kb on - strand at 254.219 kb on - strand at 254.229 kb on - strand at 254.229 kb on - strand at 254.273 kb on + strand at 254.274 kb on - strand at 254.426 kb on + strand at 254.426 kb on + strand at 254.426 kb on + strand at 254.427 kb on - strand at 254.546 kb on + strand, within GFF3431 at 254.546 kb on + strand, within GFF3431 at 254.567 kb on + strand, within GFF3431 at 254.567 kb on + strand, within GFF3431 at 254.567 kb on + strand, within GFF3431 at 254.568 kb on - strand, within GFF3431 at 254.568 kb on - strand, within GFF3431 at 254.568 kb on - strand, within GFF3431 at 254.568 kb on - strand, within GFF3431 at 254.576 kb on + strand, within GFF3431 at 254.577 kb on - strand, within GFF3431
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 252,294 + +1.3 252,305 + -0.0 252,305 + +1.2 252,731 + GFF3429 0.45 +1.1 252,731 + GFF3429 0.45 +0.7 252,732 - GFF3429 0.45 +0.0 252,740 + GFF3429 0.46 -0.2 252,741 - GFF3429 0.46 +1.0 253,058 + GFF3429 0.81 -0.1 253,058 + GFF3429 0.81 -0.0 253,109 + GFF3429 0.86 +1.2 253,109 + GFF3429 0.86 +0.4 253,110 - GFF3429 0.87 -0.3 253,110 - GFF3429 0.87 -1.0 253,326 + -0.8 253,326 + -0.1 253,326 + -0.2 253,326 + +0.9 253,327 - +0.1 253,327 - +1.0 253,327 - +0.9 253,329 + +0.8 253,329 + +0.7 253,329 + +0.7 253,329 + -0.1 253,329 + -0.1 253,329 + +0.1 253,329 + -1.7 253,329 + +0.1 253,330 - +1.0 253,330 - -0.9 253,330 - -1.4 253,330 - -1.0 253,330 - -0.6 253,470 + GFF3430 0.25 -0.0 253,470 + GFF3430 0.25 +0.4 253,471 - GFF3430 0.25 -0.1 253,704 + GFF3430 0.51 -0.1 253,704 + GFF3430 0.51 -0.1 253,705 - GFF3430 0.51 -0.1 253,705 - GFF3430 0.51 +1.5 253,938 + GFF3430 0.77 -1.0 253,938 + GFF3430 0.77 +0.7 253,938 + GFF3430 0.77 -1.0 253,938 + GFF3430 0.77 +1.7 253,938 + GFF3430 0.77 -0.3 254,076 + -1.4 254,076 + -1.1 254,076 + +0.1 254,076 + -0.5 254,076 + -0.6 254,077 - +0.5 254,077 - -0.8 254,077 - -0.6 254,077 - +0.0 254,077 - -0.3 254,145 + -0.4 254,145 + -0.4 254,145 + +0.2 254,145 + +0.6 254,145 + -1.5 254,145 + -1.6 254,145 + -0.7 254,145 + +0.3 254,145 + -1.4 254,145 + +0.1 254,145 + -1.7 254,146 - -0.4 254,146 - -0.1 254,146 - +0.1 254,146 - +0.4 254,146 - +0.3 254,158 + -1.3 254,218 + +0.2 254,219 - -0.2 254,219 - +0.1 254,229 - +1.9 254,229 - -1.0 254,273 + -0.8 254,274 - -0.0 254,426 + +1.1 254,426 + -1.1 254,426 + -0.1 254,427 - -2.1 254,546 + GFF3431 0.18 +1.5 254,546 + GFF3431 0.18 -0.4 254,567 + GFF3431 0.20 -0.8 254,567 + GFF3431 0.20 +0.0 254,567 + GFF3431 0.20 -1.4 254,568 - GFF3431 0.20 +0.0 254,568 - GFF3431 0.20 -0.8 254,568 - GFF3431 0.20 -0.8 254,568 - GFF3431 0.20 +0.5 254,576 + GFF3431 0.20 -0.4 254,577 - GFF3431 0.21 -0.1
Or see this region's nucleotide sequence