Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3425 and GFF3426 overlap by 4 nucleotides GFF3426 and GFF3427 overlap by 4 nucleotides GFF3427 and GFF3428 are separated by 4 nucleotides GFF3428 and GFF3429 are separated by 16 nucleotides
GFF3425 - no description, at 249,814 to 250,161
GFF3425
GFF3426 - Predicted kinase, at 250,158 to 250,973
GFF3426
GFF3427 - FIG01006093: hypothetical protein, at 250,970 to 251,479
GFF3427
GFF3428 - 2-oxo-hepta-3-ene-1,7-dioic acid hydratase (EC 4.2.-.-), at 251,484 to 252,296
GFF3428
GFF3429 - Transcriptional regulator, AraC family, at 252,313 to 253,233
GFF3429
Position (kb) 250
251
252 Strain fitness (log2 ratio) -2
-1
0
1
2 at 250.057 kb on + strand, within GFF3425 at 250.057 kb on + strand, within GFF3425 at 250.058 kb on - strand, within GFF3425 at 250.269 kb on + strand, within GFF3426 at 250.269 kb on + strand, within GFF3426 at 250.569 kb on + strand, within GFF3426 at 250.569 kb on + strand, within GFF3426 at 251.282 kb on + strand, within GFF3427 at 251.283 kb on - strand, within GFF3427 at 251.283 kb on - strand, within GFF3427 at 251.436 kb on + strand at 251.444 kb on + strand at 251.445 kb on - strand at 251.445 kb on - strand at 251.511 kb on + strand at 251.512 kb on - strand at 251.637 kb on + strand, within GFF3428 at 251.676 kb on + strand, within GFF3428 at 251.685 kb on + strand, within GFF3428 at 251.685 kb on + strand, within GFF3428 at 251.685 kb on + strand, within GFF3428 at 251.685 kb on + strand, within GFF3428 at 251.685 kb on + strand, within GFF3428 at 251.685 kb on + strand, within GFF3428 at 251.685 kb on + strand, within GFF3428 at 251.685 kb on + strand, within GFF3428 at 251.685 kb on + strand, within GFF3428 at 251.686 kb on - strand, within GFF3428 at 251.686 kb on - strand, within GFF3428 at 251.686 kb on - strand, within GFF3428 at 252.060 kb on + strand, within GFF3428 at 252.294 kb on + strand at 252.305 kb on + strand at 252.305 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 250,057 + GFF3425 0.70 -0.9 250,057 + GFF3425 0.70 -0.1 250,058 - GFF3425 0.70 -0.1 250,269 + GFF3426 0.14 -0.6 250,269 + GFF3426 0.14 -1.4 250,569 + GFF3426 0.50 +0.2 250,569 + GFF3426 0.50 -1.8 251,282 + GFF3427 0.61 +0.7 251,283 - GFF3427 0.61 -1.3 251,283 - GFF3427 0.61 -0.4 251,436 + +1.0 251,444 + -0.4 251,445 - -2.1 251,445 - +0.6 251,511 + -0.9 251,512 - -0.8 251,637 + GFF3428 0.19 -1.3 251,676 + GFF3428 0.24 +0.8 251,685 + GFF3428 0.25 -1.6 251,685 + GFF3428 0.25 +0.5 251,685 + GFF3428 0.25 +0.6 251,685 + GFF3428 0.25 -0.1 251,685 + GFF3428 0.25 -1.1 251,685 + GFF3428 0.25 -0.2 251,685 + GFF3428 0.25 +0.0 251,685 + GFF3428 0.25 -0.0 251,685 + GFF3428 0.25 -0.5 251,686 - GFF3428 0.25 +0.1 251,686 - GFF3428 0.25 -0.6 251,686 - GFF3428 0.25 -0.5 252,060 + GFF3428 0.71 +2.5 252,294 + +1.3 252,305 + -0.0 252,305 + +1.2
Or see this region's nucleotide sequence