Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3396 and GFF3397 are separated by 150 nucleotides GFF3397 and GFF3398 overlap by 4 nucleotides GFF3398 and GFF3399 are separated by 142 nucleotides
GFF3396 - Benzoate ABC transporter, permease protein 1, at 218,658 to 219,530
GFF3396
GFF3397 - Benzoate ABC transporter, substrate-binding protein, at 219,681 to 220,853
GFF3397
GFF3398 - benzoate degradation ring-cleavage hydrolase, at 220,850 to 221,659
GFF3398
GFF3399 - Benzoate--CoA ligase (EC 6.2.1.25), at 221,802 to 223,349
GFF3399
Position (kb)
219
220
221 Strain fitness (log2 ratio)
-1
0
1 at 218.719 kb on + strand at 218.719 kb on + strand at 218.719 kb on + strand at 218.719 kb on + strand at 218.720 kb on - strand at 218.953 kb on + strand, within GFF3396 at 218.953 kb on + strand, within GFF3396 at 219.052 kb on + strand, within GFF3396 at 219.052 kb on + strand, within GFF3396 at 219.053 kb on - strand, within GFF3396 at 219.109 kb on + strand, within GFF3396 at 219.109 kb on + strand, within GFF3396 at 219.110 kb on - strand, within GFF3396 at 219.226 kb on + strand, within GFF3396 at 219.227 kb on - strand, within GFF3396 at 219.263 kb on - strand, within GFF3396 at 219.268 kb on + strand, within GFF3396 at 219.268 kb on + strand, within GFF3396 at 219.268 kb on + strand, within GFF3396 at 219.269 kb on - strand, within GFF3396 at 219.269 kb on - strand, within GFF3396 at 219.295 kb on + strand, within GFF3396 at 219.382 kb on + strand, within GFF3396 at 219.382 kb on + strand, within GFF3396 at 219.382 kb on + strand, within GFF3396 at 219.383 kb on - strand, within GFF3396 at 219.383 kb on - strand, within GFF3396 at 219.383 kb on - strand, within GFF3396 at 219.397 kb on + strand, within GFF3396 at 219.397 kb on + strand, within GFF3396 at 219.397 kb on + strand, within GFF3396 at 219.398 kb on - strand, within GFF3396 at 219.682 kb on + strand at 219.682 kb on + strand at 219.682 kb on + strand at 219.683 kb on - strand at 219.683 kb on - strand at 219.683 kb on - strand at 219.769 kb on + strand at 219.769 kb on + strand at 219.906 kb on + strand, within GFF3397 at 219.906 kb on + strand, within GFF3397 at 219.906 kb on + strand, within GFF3397 at 219.907 kb on - strand, within GFF3397 at 219.907 kb on - strand, within GFF3397 at 219.925 kb on + strand, within GFF3397 at 219.940 kb on + strand, within GFF3397 at 219.940 kb on + strand, within GFF3397 at 219.973 kb on + strand, within GFF3397 at 220.022 kb on - strand, within GFF3397 at 220.180 kb on + strand, within GFF3397 at 220.180 kb on + strand, within GFF3397 at 220.181 kb on - strand, within GFF3397 at 220.330 kb on + strand, within GFF3397 at 220.396 kb on + strand, within GFF3397 at 220.642 kb on + strand, within GFF3397 at 220.642 kb on + strand, within GFF3397 at 220.643 kb on - strand, within GFF3397 at 220.687 kb on + strand, within GFF3397 at 220.729 kb on + strand, within GFF3397 at 220.729 kb on + strand, within GFF3397 at 220.729 kb on + strand, within GFF3397 at 220.730 kb on - strand, within GFF3397 at 220.730 kb on - strand, within GFF3397 at 220.730 kb on - strand, within GFF3397 at 221.014 kb on - strand, within GFF3398 at 221.188 kb on - strand, within GFF3398 at 221.292 kb on + strand, within GFF3398 at 221.292 kb on + strand, within GFF3398 at 221.292 kb on + strand, within GFF3398 at 221.292 kb on + strand, within GFF3398 at 221.293 kb on - strand, within GFF3398
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 218,719 + -0.6 218,719 + -0.2 218,719 + -0.2 218,719 + +0.2 218,720 - +0.4 218,953 + GFF3396 0.34 -1.3 218,953 + GFF3396 0.34 -1.5 219,052 + GFF3396 0.45 -0.2 219,052 + GFF3396 0.45 -0.4 219,053 - GFF3396 0.45 -0.8 219,109 + GFF3396 0.52 +0.3 219,109 + GFF3396 0.52 +0.5 219,110 - GFF3396 0.52 -0.9 219,226 + GFF3396 0.65 +0.9 219,227 - GFF3396 0.65 +1.1 219,263 - GFF3396 0.69 -0.2 219,268 + GFF3396 0.70 -0.9 219,268 + GFF3396 0.70 +0.7 219,268 + GFF3396 0.70 +1.0 219,269 - GFF3396 0.70 +0.1 219,269 - GFF3396 0.70 -0.4 219,295 + GFF3396 0.73 +1.6 219,382 + GFF3396 0.83 +0.1 219,382 + GFF3396 0.83 -0.1 219,382 + GFF3396 0.83 -1.3 219,383 - GFF3396 0.83 +0.0 219,383 - GFF3396 0.83 -0.1 219,383 - GFF3396 0.83 -0.9 219,397 + GFF3396 0.85 +0.2 219,397 + GFF3396 0.85 -0.0 219,397 + GFF3396 0.85 -0.3 219,398 - GFF3396 0.85 +0.2 219,682 + +0.0 219,682 + +0.8 219,682 + -1.7 219,683 - -0.5 219,683 - -0.8 219,683 - -0.3 219,769 + -0.1 219,769 + +0.7 219,906 + GFF3397 0.19 +0.1 219,906 + GFF3397 0.19 +0.8 219,906 + GFF3397 0.19 -0.2 219,907 - GFF3397 0.19 +0.0 219,907 - GFF3397 0.19 -1.4 219,925 + GFF3397 0.21 +0.5 219,940 + GFF3397 0.22 +0.2 219,940 + GFF3397 0.22 +0.1 219,973 + GFF3397 0.25 +0.6 220,022 - GFF3397 0.29 -0.8 220,180 + GFF3397 0.43 +0.3 220,180 + GFF3397 0.43 +0.8 220,181 - GFF3397 0.43 -0.2 220,330 + GFF3397 0.55 -0.1 220,396 + GFF3397 0.61 -0.2 220,642 + GFF3397 0.82 -0.1 220,642 + GFF3397 0.82 +0.3 220,643 - GFF3397 0.82 -0.1 220,687 + GFF3397 0.86 +1.2 220,729 + GFF3397 0.89 +1.5 220,729 + GFF3397 0.89 +0.6 220,729 + GFF3397 0.89 +0.3 220,730 - GFF3397 0.89 -0.5 220,730 - GFF3397 0.89 +0.1 220,730 - GFF3397 0.89 -0.7 221,014 - GFF3398 0.20 -1.4 221,188 - GFF3398 0.42 -0.1 221,292 + GFF3398 0.55 -0.0 221,292 + GFF3398 0.55 +0.4 221,292 + GFF3398 0.55 -0.2 221,292 + GFF3398 0.55 +0.7 221,293 - GFF3398 0.55 -0.3
Or see this region's nucleotide sequence