Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF303 and GFF304 are separated by 117 nucleotides GFF304 and GFF305 are separated by 2 nucleotides GFF305 and GFF306 are separated by 55 nucleotides GFF306 and GFF307 overlap by 34 nucleotides
GFF303 - Probable transcriptional regulatory protein YebC, at 336,543 to 337,268
GFF303
GFF304 - Ubiquinone/menaquinone biosynthesis methyltransferase family protein, at 337,386 to 338,174
GFF304
GFF305 - uncharacterized domain / RidA/YER057c/UK114 superfamily, group 5, at 338,177 to 339,214
GFF305
GFF306 - FIG018329: 1-acyl-sn-glycerol-3-phosphate acyltransferase, at 339,270 to 340,067
GFF306
GFF307 - FIG143263: Glycosyl transferase, at 340,034 to 340,810
GFF307
Position (kb) 338
339
340 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 337.241 kb on + strand at 337.241 kb on + strand at 337.241 kb on + strand at 337.241 kb on + strand at 337.242 kb on - strand at 337.242 kb on - strand at 337.242 kb on - strand at 337.256 kb on + strand at 337.256 kb on + strand at 337.256 kb on + strand at 337.256 kb on + strand at 337.257 kb on - strand at 337.257 kb on - strand at 337.257 kb on - strand at 337.257 kb on - strand at 337.257 kb on - strand at 337.276 kb on + strand at 337.276 kb on + strand at 337.277 kb on - strand at 337.315 kb on + strand at 337.315 kb on + strand at 337.315 kb on + strand at 337.315 kb on + strand at 337.316 kb on - strand at 337.316 kb on - strand at 337.316 kb on - strand at 337.376 kb on + strand at 337.376 kb on + strand at 337.376 kb on + strand at 337.376 kb on + strand at 337.376 kb on + strand at 337.376 kb on + strand at 337.377 kb on - strand at 337.380 kb on - strand at 337.455 kb on + strand at 337.455 kb on + strand at 337.456 kb on - strand at 337.456 kb on - strand at 337.456 kb on - strand at 337.456 kb on - strand at 337.458 kb on + strand at 337.458 kb on + strand at 337.459 kb on - strand at 337.459 kb on - strand at 337.459 kb on - strand at 337.459 kb on - strand at 337.459 kb on - strand at 337.459 kb on - strand at 337.459 kb on - strand at 337.521 kb on + strand, within GFF304 at 337.521 kb on + strand, within GFF304 at 337.522 kb on - strand, within GFF304 at 337.566 kb on + strand, within GFF304 at 337.812 kb on + strand, within GFF304 at 337.812 kb on + strand, within GFF304 at 337.812 kb on + strand, within GFF304 at 337.812 kb on + strand, within GFF304 at 337.905 kb on - strand, within GFF304 at 337.947 kb on + strand, within GFF304 at 337.947 kb on + strand, within GFF304 at 337.947 kb on + strand, within GFF304 at 337.947 kb on + strand, within GFF304 at 337.947 kb on + strand, within GFF304 at 337.947 kb on + strand, within GFF304 at 337.947 kb on + strand, within GFF304 at 337.947 kb on + strand, within GFF304 at 337.947 kb on + strand, within GFF304 at 337.948 kb on - strand, within GFF304 at 337.948 kb on - strand, within GFF304 at 337.948 kb on - strand, within GFF304 at 337.948 kb on - strand, within GFF304 at 337.948 kb on - strand, within GFF304 at 337.948 kb on - strand, within GFF304 at 337.948 kb on - strand, within GFF304 at 338.019 kb on + strand, within GFF304 at 338.019 kb on + strand, within GFF304 at 338.020 kb on - strand, within GFF304 at 338.020 kb on - strand, within GFF304 at 338.020 kb on - strand, within GFF304 at 338.023 kb on - strand, within GFF304 at 338.133 kb on + strand at 338.133 kb on + strand at 338.134 kb on - strand at 338.134 kb on - strand at 338.135 kb on + strand at 338.135 kb on + strand at 338.135 kb on + strand at 338.136 kb on - strand at 338.156 kb on + strand at 338.157 kb on - strand at 338.232 kb on - strand at 338.291 kb on + strand, within GFF305 at 338.392 kb on + strand, within GFF305 at 338.393 kb on - strand, within GFF305 at 338.393 kb on - strand, within GFF305 at 338.513 kb on + strand, within GFF305 at 338.514 kb on - strand, within GFF305 at 338.514 kb on - strand, within GFF305 at 338.514 kb on - strand, within GFF305 at 338.514 kb on - strand, within GFF305 at 338.576 kb on + strand, within GFF305 at 338.576 kb on + strand, within GFF305 at 338.576 kb on + strand, within GFF305 at 338.576 kb on + strand, within GFF305 at 338.576 kb on + strand, within GFF305 at 338.577 kb on - strand, within GFF305 at 338.577 kb on - strand, within GFF305 at 338.577 kb on - strand, within GFF305 at 338.577 kb on - strand, within GFF305 at 338.577 kb on - strand, within GFF305 at 338.577 kb on - strand, within GFF305 at 338.766 kb on + strand, within GFF305 at 338.768 kb on + strand, within GFF305 at 338.792 kb on + strand, within GFF305 at 338.810 kb on + strand, within GFF305 at 338.810 kb on + strand, within GFF305 at 338.810 kb on + strand, within GFF305 at 338.811 kb on - strand, within GFF305 at 338.811 kb on - strand, within GFF305 at 338.811 kb on - strand, within GFF305 at 339.041 kb on + strand, within GFF305 at 339.041 kb on + strand, within GFF305 at 339.041 kb on + strand, within GFF305 at 339.041 kb on + strand, within GFF305 at 339.041 kb on + strand, within GFF305 at 339.041 kb on + strand, within GFF305 at 339.041 kb on + strand, within GFF305 at 339.041 kb on + strand, within GFF305 at 339.042 kb on - strand, within GFF305 at 339.042 kb on - strand, within GFF305 at 339.042 kb on - strand, within GFF305 at 339.042 kb on - strand, within GFF305 at 339.042 kb on - strand, within GFF305 at 339.042 kb on - strand, within GFF305 at 339.042 kb on - strand, within GFF305 at 339.042 kb on - strand, within GFF305 at 339.045 kb on + strand, within GFF305 at 339.045 kb on + strand, within GFF305 at 339.045 kb on + strand, within GFF305 at 339.045 kb on + strand, within GFF305 at 339.045 kb on + strand, within GFF305 at 339.045 kb on + strand, within GFF305 at 339.045 kb on + strand, within GFF305 at 339.045 kb on + strand, within GFF305 at 339.046 kb on - strand, within GFF305 at 339.046 kb on - strand, within GFF305 at 339.046 kb on - strand, within GFF305 at 339.046 kb on - strand, within GFF305 at 339.046 kb on - strand, within GFF305 at 339.097 kb on - strand, within GFF305 at 339.131 kb on + strand at 339.131 kb on + strand at 339.132 kb on - strand at 339.132 kb on - strand at 339.132 kb on - strand at 339.322 kb on - strand at 339.384 kb on + strand, within GFF306 at 339.384 kb on + strand, within GFF306 at 339.384 kb on + strand, within GFF306 at 339.385 kb on - strand, within GFF306 at 339.444 kb on + strand, within GFF306 at 339.445 kb on - strand, within GFF306 at 339.666 kb on + strand, within GFF306 at 339.666 kb on + strand, within GFF306 at 339.666 kb on + strand, within GFF306 at 339.666 kb on + strand, within GFF306 at 339.666 kb on + strand, within GFF306 at 339.667 kb on - strand, within GFF306 at 339.667 kb on - strand, within GFF306 at 339.667 kb on - strand, within GFF306 at 339.667 kb on - strand, within GFF306 at 339.667 kb on - strand, within GFF306 at 339.667 kb on - strand, within GFF306 at 339.667 kb on - strand, within GFF306 at 339.667 kb on - strand, within GFF306 at 339.667 kb on - strand, within GFF306 at 339.667 kb on - strand, within GFF306 at 339.865 kb on - strand, within GFF306 at 339.865 kb on - strand, within GFF306 at 339.987 kb on + strand, within GFF306 at 339.987 kb on + strand, within GFF306 at 339.987 kb on + strand, within GFF306 at 339.987 kb on + strand, within GFF306 at 339.987 kb on + strand, within GFF306 at 339.987 kb on + strand, within GFF306 at 339.987 kb on + strand, within GFF306 at 339.988 kb on - strand at 339.988 kb on - strand at 339.988 kb on - strand at 340.076 kb on + strand at 340.076 kb on + strand at 340.076 kb on + strand at 340.076 kb on + strand at 340.077 kb on - strand at 340.077 kb on - strand at 340.077 kb on - strand at 340.077 kb on - strand at 340.137 kb on + strand, within GFF307
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 337,241 + -1.4 337,241 + -1.4 337,241 + -0.4 337,241 + -3.0 337,242 - -1.1 337,242 - -1.2 337,242 - -1.2 337,256 + -0.9 337,256 + +0.5 337,256 + -0.4 337,256 + -0.8 337,257 - +0.5 337,257 - -0.3 337,257 - -0.7 337,257 - -0.4 337,257 - -2.2 337,276 + -1.8 337,276 + +0.8 337,277 - -0.7 337,315 + +0.5 337,315 + +0.7 337,315 + +0.2 337,315 + -0.2 337,316 - -0.4 337,316 - -1.4 337,316 - +0.7 337,376 + -0.2 337,376 + -1.1 337,376 + +0.7 337,376 + -0.8 337,376 + +0.4 337,376 + +0.4 337,377 - +0.1 337,380 - +0.6 337,455 + -0.3 337,455 + -0.6 337,456 - -0.5 337,456 - -1.2 337,456 - +0.2 337,456 - -0.0 337,458 + -1.6 337,458 + -0.2 337,459 - +0.8 337,459 - -0.5 337,459 - +0.9 337,459 - -0.2 337,459 - -0.2 337,459 - -1.0 337,459 - -0.3 337,521 + GFF304 0.17 +0.4 337,521 + GFF304 0.17 -1.8 337,522 - GFF304 0.17 -0.4 337,566 + GFF304 0.23 -0.6 337,812 + GFF304 0.54 -0.7 337,812 + GFF304 0.54 +0.6 337,812 + GFF304 0.54 +0.0 337,812 + GFF304 0.54 -0.5 337,905 - GFF304 0.66 -0.5 337,947 + GFF304 0.71 +1.0 337,947 + GFF304 0.71 -0.0 337,947 + GFF304 0.71 -0.2 337,947 + GFF304 0.71 -0.1 337,947 + GFF304 0.71 -1.0 337,947 + GFF304 0.71 -0.4 337,947 + GFF304 0.71 -0.4 337,947 + GFF304 0.71 +2.1 337,947 + GFF304 0.71 -0.5 337,948 - GFF304 0.71 +0.6 337,948 - GFF304 0.71 +0.3 337,948 - GFF304 0.71 -1.2 337,948 - GFF304 0.71 -0.8 337,948 - GFF304 0.71 -0.5 337,948 - GFF304 0.71 -0.3 337,948 - GFF304 0.71 +0.2 338,019 + GFF304 0.80 +1.0 338,019 + GFF304 0.80 -0.2 338,020 - GFF304 0.80 +0.4 338,020 - GFF304 0.80 -0.8 338,020 - GFF304 0.80 -0.6 338,023 - GFF304 0.81 -1.1 338,133 + -1.4 338,133 + +0.4 338,134 - -0.9 338,134 - -0.2 338,135 + -1.1 338,135 + +0.2 338,135 + -0.1 338,136 - +1.4 338,156 + -0.2 338,157 - +0.2 338,232 - -1.2 338,291 + GFF305 0.11 -1.7 338,392 + GFF305 0.21 +0.2 338,393 - GFF305 0.21 -2.4 338,393 - GFF305 0.21 -1.7 338,513 + GFF305 0.32 -1.1 338,514 - GFF305 0.32 -2.2 338,514 - GFF305 0.32 -0.2 338,514 - GFF305 0.32 -0.9 338,514 - GFF305 0.32 -0.5 338,576 + GFF305 0.38 -1.8 338,576 + GFF305 0.38 +0.5 338,576 + GFF305 0.38 -0.2 338,576 + GFF305 0.38 +1.4 338,576 + GFF305 0.38 -0.8 338,577 - GFF305 0.39 +0.6 338,577 - GFF305 0.39 -0.4 338,577 - GFF305 0.39 -2.3 338,577 - GFF305 0.39 -1.0 338,577 - GFF305 0.39 -0.8 338,577 - GFF305 0.39 -1.3 338,766 + GFF305 0.57 -0.6 338,768 + GFF305 0.57 -0.8 338,792 + GFF305 0.59 -2.3 338,810 + GFF305 0.61 +0.4 338,810 + GFF305 0.61 -0.4 338,810 + GFF305 0.61 -2.1 338,811 - GFF305 0.61 -0.9 338,811 - GFF305 0.61 -0.6 338,811 - GFF305 0.61 -0.6 339,041 + GFF305 0.83 -0.7 339,041 + GFF305 0.83 +0.2 339,041 + GFF305 0.83 -0.8 339,041 + GFF305 0.83 +0.4 339,041 + GFF305 0.83 -0.5 339,041 + GFF305 0.83 -0.5 339,041 + GFF305 0.83 -1.6 339,041 + GFF305 0.83 +1.4 339,042 - GFF305 0.83 -0.3 339,042 - GFF305 0.83 -1.2 339,042 - GFF305 0.83 -0.6 339,042 - GFF305 0.83 -0.9 339,042 - GFF305 0.83 -0.9 339,042 - GFF305 0.83 -0.0 339,042 - GFF305 0.83 -0.9 339,042 - GFF305 0.83 -0.4 339,045 + GFF305 0.84 -0.9 339,045 + GFF305 0.84 -1.0 339,045 + GFF305 0.84 +0.1 339,045 + GFF305 0.84 -1.2 339,045 + GFF305 0.84 -1.8 339,045 + GFF305 0.84 -0.2 339,045 + GFF305 0.84 -1.7 339,045 + GFF305 0.84 -1.0 339,046 - GFF305 0.84 +0.4 339,046 - GFF305 0.84 -0.9 339,046 - GFF305 0.84 -1.1 339,046 - GFF305 0.84 +0.4 339,046 - GFF305 0.84 -0.3 339,097 - GFF305 0.89 -0.2 339,131 + +0.2 339,131 + -0.0 339,132 - -0.6 339,132 - +0.4 339,132 - -0.1 339,322 - +0.0 339,384 + GFF306 0.14 +0.1 339,384 + GFF306 0.14 +1.3 339,384 + GFF306 0.14 -1.9 339,385 - GFF306 0.14 -0.8 339,444 + GFF306 0.22 -1.4 339,445 - GFF306 0.22 -0.6 339,666 + GFF306 0.50 -0.0 339,666 + GFF306 0.50 -0.4 339,666 + GFF306 0.50 -0.3 339,666 + GFF306 0.50 -0.4 339,666 + GFF306 0.50 +0.6 339,667 - GFF306 0.50 -0.2 339,667 - GFF306 0.50 -0.4 339,667 - GFF306 0.50 -0.8 339,667 - GFF306 0.50 +0.6 339,667 - GFF306 0.50 -0.4 339,667 - GFF306 0.50 -0.4 339,667 - GFF306 0.50 -0.1 339,667 - GFF306 0.50 -0.1 339,667 - GFF306 0.50 -0.3 339,667 - GFF306 0.50 +0.7 339,865 - GFF306 0.75 -1.8 339,865 - GFF306 0.75 +0.3 339,987 + GFF306 0.90 +1.4 339,987 + GFF306 0.90 +0.9 339,987 + GFF306 0.90 +0.4 339,987 + GFF306 0.90 -1.3 339,987 + GFF306 0.90 -0.6 339,987 + GFF306 0.90 -0.1 339,987 + GFF306 0.90 -1.1 339,988 - +0.9 339,988 - -0.3 339,988 - +0.6 340,076 + +0.2 340,076 + -0.5 340,076 + +1.8 340,076 + +1.0 340,077 - -1.2 340,077 - +1.8 340,077 - -0.4 340,077 - +1.1 340,137 + GFF307 0.13 -0.3
Or see this region's nucleotide sequence