Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2995 and GFF2996 are separated by 14 nucleotides GFF2996 and GFF2997 are separated by 113 nucleotides GFF2997 and GFF2998 are separated by 2 nucleotides GFF2998 and GFF2999 are separated by 169 nucleotides GFF2999 and GFF3000 overlap by 4 nucleotides
GFF2995 - hypothetical protein, at 426,617 to 427,279
GFF2995
GFF2996 - Thiol-disulfide isomerase and thioredoxins, at 427,294 to 427,677
GFF2996
GFF2997 - FIG027190: Putative transmembrane protein, at 427,791 to 428,129
GFF2997
GFF2998 - Virulence factor BrkB, at 428,132 to 429,343
GFF2998
GFF2999 - Putative transmembrane protein, at 429,513 to 429,875
GFF2999
GFF3000 - D-2-hydroxyglutarate dehydrogenase (EC 1.1.99.2), at 429,872 to 431,299
GFF3000
Position (kb) 428
429
430 Strain fitness (log2 ratio) -2
-1
0
1
2 at 427.184 kb on + strand, within GFF2995 at 427.184 kb on + strand, within GFF2995 at 427.184 kb on + strand, within GFF2995 at 427.185 kb on - strand, within GFF2995 at 427.736 kb on + strand at 427.736 kb on + strand at 427.736 kb on + strand at 427.736 kb on + strand at 427.736 kb on + strand at 427.737 kb on - strand at 427.857 kb on + strand, within GFF2997 at 427.857 kb on + strand, within GFF2997 at 427.857 kb on + strand, within GFF2997 at 427.857 kb on + strand, within GFF2997 at 427.858 kb on - strand, within GFF2997 at 427.858 kb on - strand, within GFF2997 at 427.866 kb on + strand, within GFF2997 at 427.866 kb on + strand, within GFF2997 at 427.867 kb on - strand, within GFF2997 at 428.067 kb on + strand, within GFF2997 at 428.068 kb on - strand, within GFF2997 at 428.068 kb on - strand, within GFF2997 at 428.068 kb on - strand, within GFF2997 at 428.094 kb on + strand, within GFF2997 at 428.094 kb on + strand, within GFF2997 at 428.094 kb on + strand, within GFF2997 at 428.127 kb on + strand at 428.127 kb on + strand at 428.127 kb on + strand at 428.127 kb on + strand at 428.127 kb on + strand at 428.128 kb on - strand at 428.128 kb on - strand at 428.128 kb on - strand at 428.128 kb on - strand at 428.134 kb on - strand at 428.134 kb on - strand at 428.134 kb on - strand at 428.271 kb on + strand, within GFF2998 at 428.271 kb on + strand, within GFF2998 at 428.271 kb on + strand, within GFF2998 at 428.271 kb on + strand, within GFF2998 at 428.272 kb on - strand, within GFF2998 at 428.272 kb on - strand, within GFF2998 at 428.520 kb on + strand, within GFF2998 at 428.520 kb on + strand, within GFF2998 at 428.521 kb on - strand, within GFF2998 at 428.565 kb on + strand, within GFF2998 at 428.565 kb on + strand, within GFF2998 at 428.565 kb on + strand, within GFF2998 at 428.565 kb on + strand, within GFF2998 at 428.566 kb on - strand, within GFF2998 at 428.566 kb on - strand, within GFF2998 at 428.566 kb on - strand, within GFF2998 at 428.566 kb on - strand, within GFF2998 at 428.566 kb on - strand, within GFF2998 at 428.608 kb on - strand, within GFF2998 at 428.619 kb on + strand, within GFF2998 at 428.619 kb on + strand, within GFF2998 at 428.620 kb on - strand, within GFF2998 at 428.620 kb on - strand, within GFF2998 at 428.620 kb on - strand, within GFF2998 at 428.640 kb on + strand, within GFF2998 at 428.640 kb on + strand, within GFF2998 at 428.640 kb on + strand, within GFF2998 at 428.640 kb on + strand, within GFF2998 at 428.641 kb on - strand, within GFF2998 at 428.641 kb on - strand, within GFF2998 at 428.641 kb on - strand, within GFF2998 at 428.641 kb on - strand, within GFF2998 at 428.643 kb on + strand, within GFF2998 at 428.643 kb on + strand, within GFF2998 at 428.643 kb on + strand, within GFF2998 at 428.644 kb on - strand, within GFF2998 at 428.736 kb on + strand, within GFF2998 at 428.736 kb on + strand, within GFF2998 at 428.736 kb on + strand, within GFF2998 at 428.736 kb on + strand, within GFF2998 at 428.737 kb on - strand, within GFF2998 at 428.737 kb on - strand, within GFF2998 at 428.737 kb on - strand, within GFF2998 at 428.737 kb on - strand, within GFF2998 at 428.737 kb on - strand, within GFF2998 at 428.737 kb on - strand, within GFF2998 at 428.742 kb on + strand, within GFF2998 at 428.742 kb on + strand, within GFF2998 at 428.742 kb on + strand, within GFF2998 at 428.742 kb on + strand, within GFF2998 at 428.844 kb on + strand, within GFF2998 at 428.844 kb on + strand, within GFF2998 at 428.844 kb on + strand, within GFF2998 at 428.844 kb on + strand, within GFF2998 at 428.844 kb on + strand, within GFF2998 at 428.844 kb on + strand, within GFF2998 at 428.844 kb on + strand, within GFF2998 at 428.844 kb on + strand, within GFF2998 at 428.845 kb on - strand, within GFF2998 at 428.845 kb on - strand, within GFF2998 at 428.845 kb on - strand, within GFF2998 at 428.845 kb on - strand, within GFF2998 at 428.845 kb on - strand, within GFF2998 at 428.845 kb on - strand, within GFF2998 at 428.845 kb on - strand, within GFF2998 at 428.845 kb on - strand, within GFF2998 at 428.845 kb on - strand, within GFF2998 at 428.845 kb on - strand, within GFF2998 at 428.845 kb on - strand, within GFF2998 at 428.845 kb on - strand, within GFF2998 at 428.904 kb on + strand, within GFF2998 at 428.905 kb on - strand, within GFF2998 at 429.270 kb on - strand at 429.338 kb on + strand at 429.339 kb on - strand at 429.343 kb on + strand at 429.343 kb on + strand at 429.343 kb on + strand at 429.343 kb on + strand at 429.343 kb on + strand at 429.343 kb on + strand at 429.343 kb on + strand at 429.343 kb on + strand at 429.343 kb on + strand at 429.344 kb on - strand at 429.344 kb on - strand at 429.344 kb on - strand at 429.344 kb on - strand at 429.344 kb on - strand at 429.654 kb on + strand, within GFF2999 at 429.654 kb on + strand, within GFF2999 at 429.654 kb on + strand, within GFF2999 at 429.654 kb on + strand, within GFF2999 at 429.654 kb on + strand, within GFF2999 at 429.654 kb on + strand, within GFF2999 at 429.654 kb on + strand, within GFF2999 at 429.654 kb on + strand, within GFF2999 at 429.654 kb on + strand, within GFF2999 at 429.654 kb on + strand, within GFF2999 at 429.654 kb on + strand, within GFF2999 at 429.654 kb on + strand, within GFF2999 at 429.654 kb on + strand, within GFF2999 at 429.654 kb on + strand, within GFF2999 at 429.654 kb on + strand, within GFF2999 at 429.655 kb on - strand, within GFF2999 at 429.655 kb on - strand, within GFF2999 at 429.655 kb on - strand, within GFF2999 at 429.655 kb on - strand, within GFF2999 at 429.655 kb on - strand, within GFF2999 at 429.655 kb on - strand, within GFF2999 at 429.655 kb on - strand, within GFF2999 at 429.655 kb on - strand, within GFF2999 at 429.655 kb on - strand, within GFF2999 at 429.655 kb on - strand, within GFF2999 at 429.655 kb on - strand, within GFF2999 at 429.655 kb on - strand, within GFF2999 at 429.655 kb on - strand, within GFF2999 at 429.655 kb on - strand, within GFF2999 at 429.655 kb on - strand, within GFF2999 at 429.655 kb on - strand, within GFF2999 at 429.655 kb on - strand, within GFF2999 at 429.655 kb on - strand, within GFF2999 at 429.655 kb on - strand, within GFF2999 at 429.660 kb on + strand, within GFF2999 at 429.660 kb on + strand, within GFF2999 at 429.661 kb on - strand, within GFF2999 at 429.661 kb on - strand, within GFF2999 at 429.661 kb on - strand, within GFF2999 at 429.661 kb on - strand, within GFF2999 at 429.661 kb on - strand, within GFF2999 at 429.690 kb on + strand, within GFF2999 at 429.691 kb on - strand, within GFF2999 at 429.691 kb on - strand, within GFF2999 at 429.691 kb on - strand, within GFF2999 at 429.931 kb on - strand at 429.931 kb on - strand at 429.931 kb on - strand at 429.931 kb on - strand at 429.956 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 427,184 + GFF2995 0.86 +0.1 427,184 + GFF2995 0.86 +0.8 427,184 + GFF2995 0.86 -0.0 427,185 - GFF2995 0.86 -0.7 427,736 + +0.3 427,736 + +0.5 427,736 + +0.3 427,736 + +0.1 427,736 + -0.5 427,737 - -0.5 427,857 + GFF2997 0.19 +1.4 427,857 + GFF2997 0.19 +0.4 427,857 + GFF2997 0.19 -0.6 427,857 + GFF2997 0.19 -0.4 427,858 - GFF2997 0.20 -1.0 427,858 - GFF2997 0.20 -0.9 427,866 + GFF2997 0.22 -0.3 427,866 + GFF2997 0.22 -0.4 427,867 - GFF2997 0.22 +0.9 428,067 + GFF2997 0.81 -0.4 428,068 - GFF2997 0.82 -0.3 428,068 - GFF2997 0.82 -0.6 428,068 - GFF2997 0.82 -1.0 428,094 + GFF2997 0.89 -0.1 428,094 + GFF2997 0.89 -0.0 428,094 + GFF2997 0.89 -1.3 428,127 + +0.3 428,127 + -0.5 428,127 + -0.2 428,127 + -1.1 428,127 + -1.2 428,128 - +0.0 428,128 - +1.1 428,128 - +0.3 428,128 - +1.1 428,134 - -0.2 428,134 - -0.3 428,134 - -0.2 428,271 + GFF2998 0.11 -0.7 428,271 + GFF2998 0.11 -0.3 428,271 + GFF2998 0.11 +0.4 428,271 + GFF2998 0.11 -0.2 428,272 - GFF2998 0.12 +0.8 428,272 - GFF2998 0.12 -0.2 428,520 + GFF2998 0.32 -0.6 428,520 + GFF2998 0.32 +0.4 428,521 - GFF2998 0.32 -0.7 428,565 + GFF2998 0.36 -0.3 428,565 + GFF2998 0.36 +2.5 428,565 + GFF2998 0.36 -0.8 428,565 + GFF2998 0.36 -1.2 428,566 - GFF2998 0.36 -0.3 428,566 - GFF2998 0.36 +0.4 428,566 - GFF2998 0.36 -0.4 428,566 - GFF2998 0.36 -1.0 428,566 - GFF2998 0.36 +0.0 428,608 - GFF2998 0.39 -0.4 428,619 + GFF2998 0.40 -0.1 428,619 + GFF2998 0.40 +0.8 428,620 - GFF2998 0.40 -1.4 428,620 - GFF2998 0.40 -1.3 428,620 - GFF2998 0.40 -0.7 428,640 + GFF2998 0.42 +0.4 428,640 + GFF2998 0.42 +1.0 428,640 + GFF2998 0.42 +0.6 428,640 + GFF2998 0.42 +1.0 428,641 - GFF2998 0.42 -0.4 428,641 - GFF2998 0.42 +0.9 428,641 - GFF2998 0.42 -0.1 428,641 - GFF2998 0.42 -1.1 428,643 + GFF2998 0.42 -1.7 428,643 + GFF2998 0.42 +2.3 428,643 + GFF2998 0.42 +0.8 428,644 - GFF2998 0.42 +0.9 428,736 + GFF2998 0.50 +0.2 428,736 + GFF2998 0.50 -0.6 428,736 + GFF2998 0.50 +0.6 428,736 + GFF2998 0.50 +1.5 428,737 - GFF2998 0.50 +0.4 428,737 - GFF2998 0.50 +0.8 428,737 - GFF2998 0.50 -0.1 428,737 - GFF2998 0.50 -0.1 428,737 - GFF2998 0.50 +0.3 428,737 - GFF2998 0.50 +0.1 428,742 + GFF2998 0.50 +0.7 428,742 + GFF2998 0.50 +1.5 428,742 + GFF2998 0.50 +0.3 428,742 + GFF2998 0.50 -1.3 428,844 + GFF2998 0.59 -0.3 428,844 + GFF2998 0.59 -0.3 428,844 + GFF2998 0.59 -0.6 428,844 + GFF2998 0.59 -1.2 428,844 + GFF2998 0.59 -0.3 428,844 + GFF2998 0.59 -0.5 428,844 + GFF2998 0.59 +0.0 428,844 + GFF2998 0.59 -0.6 428,845 - GFF2998 0.59 +0.1 428,845 - GFF2998 0.59 +1.1 428,845 - GFF2998 0.59 +0.2 428,845 - GFF2998 0.59 -0.1 428,845 - GFF2998 0.59 +0.4 428,845 - GFF2998 0.59 +0.9 428,845 - GFF2998 0.59 +0.2 428,845 - GFF2998 0.59 -0.8 428,845 - GFF2998 0.59 -0.5 428,845 - GFF2998 0.59 -0.1 428,845 - GFF2998 0.59 +0.3 428,845 - GFF2998 0.59 -0.8 428,904 + GFF2998 0.64 +1.1 428,905 - GFF2998 0.64 -0.5 429,270 - -0.6 429,338 + -0.6 429,339 - -0.1 429,343 + -0.9 429,343 + -0.0 429,343 + +0.2 429,343 + +1.3 429,343 + +0.2 429,343 + -0.8 429,343 + -1.6 429,343 + -0.9 429,343 + +0.6 429,344 - -1.4 429,344 - -0.3 429,344 - +0.0 429,344 - +0.1 429,344 - -0.9 429,654 + GFF2999 0.39 +0.7 429,654 + GFF2999 0.39 -0.7 429,654 + GFF2999 0.39 -0.8 429,654 + GFF2999 0.39 -1.3 429,654 + GFF2999 0.39 +1.7 429,654 + GFF2999 0.39 +0.2 429,654 + GFF2999 0.39 -1.7 429,654 + GFF2999 0.39 -0.6 429,654 + GFF2999 0.39 -1.1 429,654 + GFF2999 0.39 -0.4 429,654 + GFF2999 0.39 -0.4 429,654 + GFF2999 0.39 -0.3 429,654 + GFF2999 0.39 +1.3 429,654 + GFF2999 0.39 -1.1 429,654 + GFF2999 0.39 +0.5 429,655 - GFF2999 0.39 -0.9 429,655 - GFF2999 0.39 -0.0 429,655 - GFF2999 0.39 -0.8 429,655 - GFF2999 0.39 +0.4 429,655 - GFF2999 0.39 -0.1 429,655 - GFF2999 0.39 +0.0 429,655 - GFF2999 0.39 +0.4 429,655 - GFF2999 0.39 -0.7 429,655 - GFF2999 0.39 -0.9 429,655 - GFF2999 0.39 -1.9 429,655 - GFF2999 0.39 +0.9 429,655 - GFF2999 0.39 -0.0 429,655 - GFF2999 0.39 -0.0 429,655 - GFF2999 0.39 +0.3 429,655 - GFF2999 0.39 -0.3 429,655 - GFF2999 0.39 -1.0 429,655 - GFF2999 0.39 -0.4 429,655 - GFF2999 0.39 +1.5 429,655 - GFF2999 0.39 +0.7 429,660 + GFF2999 0.40 -0.3 429,660 + GFF2999 0.40 -0.9 429,661 - GFF2999 0.41 -0.7 429,661 - GFF2999 0.41 -0.4 429,661 - GFF2999 0.41 +0.2 429,661 - GFF2999 0.41 -1.3 429,661 - GFF2999 0.41 +1.0 429,690 + GFF2999 0.49 -0.8 429,691 - GFF2999 0.49 -0.4 429,691 - GFF2999 0.49 -0.1 429,691 - GFF2999 0.49 -1.2 429,931 - -1.3 429,931 - -1.4 429,931 - +1.3 429,931 - -0.4 429,956 + -0.6
Or see this region's nucleotide sequence