Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2594 and GFF2595 are separated by 133 nucleotides GFF2595 and GFF2596 overlap by 4 nucleotides GFF2596 and GFF2597 overlap by 4 nucleotides GFF2597 and GFF2598 overlap by 4 nucleotides
GFF2594 - 4-hydroxyphenylpyruvate dioxygenase (EC 1.13.11.27), at 8 to 1,036
GFF2594
GFF2595 - Beta-ketoadipate enol-lactone hydrolase (EC 3.1.1.24), at 1,170 to 1,934
GFF2595
GFF2596 - Multidrug resistance transporter, Bcr/CflA family, at 1,931 to 3,151
GFF2596
GFF2597 - 4-carboxymuconolactone decarboxylase (EC 4.1.1.44), at 3,148 to 3,582
GFF2597
GFF2598 - 3-carboxy-cis,cis-muconate cycloisomerase (EC 5.5.1.2), at 3,579 to 4,793
GFF2598
Position (kb)
1
2
3
4 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1.048 kb on + strand at 1.048 kb on + strand at 1.048 kb on + strand at 1.075 kb on + strand at 1.075 kb on + strand at 1.088 kb on - strand at 1.088 kb on - strand at 1.139 kb on - strand at 1.139 kb on - strand at 1.144 kb on + strand at 1.145 kb on - strand at 1.411 kb on + strand, within GFF2595 at 1.411 kb on + strand, within GFF2595 at 1.411 kb on + strand, within GFF2595 at 1.411 kb on + strand, within GFF2595 at 1.411 kb on + strand, within GFF2595 at 1.411 kb on + strand, within GFF2595 at 1.411 kb on + strand, within GFF2595 at 1.412 kb on - strand, within GFF2595 at 1.412 kb on - strand, within GFF2595 at 1.412 kb on - strand, within GFF2595 at 1.412 kb on - strand, within GFF2595 at 1.588 kb on + strand, within GFF2595 at 1.588 kb on + strand, within GFF2595 at 1.805 kb on - strand, within GFF2595 at 2.043 kb on + strand at 2.043 kb on + strand at 2.043 kb on + strand at 2.043 kb on + strand at 2.043 kb on + strand at 2.044 kb on - strand at 2.044 kb on - strand at 2.049 kb on + strand at 2.049 kb on + strand at 2.049 kb on + strand at 2.208 kb on + strand, within GFF2596 at 2.208 kb on + strand, within GFF2596 at 2.208 kb on + strand, within GFF2596 at 2.208 kb on + strand, within GFF2596 at 2.208 kb on + strand, within GFF2596 at 2.208 kb on + strand, within GFF2596 at 2.209 kb on - strand, within GFF2596 at 2.209 kb on - strand, within GFF2596 at 2.209 kb on - strand, within GFF2596 at 2.209 kb on - strand, within GFF2596 at 2.209 kb on - strand, within GFF2596 at 2.209 kb on - strand, within GFF2596 at 2.209 kb on - strand, within GFF2596 at 2.332 kb on - strand, within GFF2596 at 2.332 kb on - strand, within GFF2596 at 2.373 kb on + strand, within GFF2596 at 2.373 kb on + strand, within GFF2596 at 2.373 kb on + strand, within GFF2596 at 2.373 kb on + strand, within GFF2596 at 2.373 kb on + strand, within GFF2596 at 2.374 kb on - strand, within GFF2596 at 2.374 kb on - strand, within GFF2596 at 2.430 kb on + strand, within GFF2596 at 2.439 kb on + strand, within GFF2596 at 2.439 kb on + strand, within GFF2596 at 2.439 kb on + strand, within GFF2596 at 2.439 kb on + strand, within GFF2596 at 2.440 kb on - strand, within GFF2596 at 2.440 kb on - strand, within GFF2596 at 2.440 kb on - strand, within GFF2596 at 2.440 kb on - strand, within GFF2596 at 2.440 kb on - strand, within GFF2596 at 2.440 kb on - strand, within GFF2596 at 2.440 kb on - strand, within GFF2596 at 2.440 kb on - strand, within GFF2596 at 2.481 kb on + strand, within GFF2596 at 2.481 kb on + strand, within GFF2596 at 2.482 kb on - strand, within GFF2596 at 2.482 kb on - strand, within GFF2596 at 2.604 kb on + strand, within GFF2596 at 2.604 kb on + strand, within GFF2596 at 2.605 kb on - strand, within GFF2596 at 2.634 kb on + strand, within GFF2596 at 2.634 kb on + strand, within GFF2596 at 2.635 kb on - strand, within GFF2596 at 2.635 kb on - strand, within GFF2596 at 2.635 kb on - strand, within GFF2596 at 2.635 kb on - strand, within GFF2596 at 2.898 kb on + strand, within GFF2596 at 2.898 kb on + strand, within GFF2596 at 2.898 kb on + strand, within GFF2596 at 2.898 kb on + strand, within GFF2596 at 2.898 kb on + strand, within GFF2596 at 2.898 kb on + strand, within GFF2596 at 2.898 kb on + strand, within GFF2596 at 2.899 kb on - strand, within GFF2596 at 2.899 kb on - strand, within GFF2596 at 2.899 kb on - strand, within GFF2596 at 2.899 kb on - strand, within GFF2596 at 2.899 kb on - strand, within GFF2596 at 2.899 kb on - strand, within GFF2596 at 2.899 kb on - strand, within GFF2596 at 2.899 kb on - strand, within GFF2596 at 2.899 kb on - strand, within GFF2596 at 2.899 kb on - strand, within GFF2596 at 2.899 kb on - strand, within GFF2596 at 3.054 kb on + strand at 3.054 kb on + strand at 3.054 kb on + strand at 3.054 kb on + strand at 3.054 kb on + strand at 3.055 kb on - strand at 3.055 kb on - strand at 3.055 kb on - strand at 3.055 kb on - strand at 3.055 kb on - strand at 3.055 kb on - strand at 3.055 kb on - strand at 3.248 kb on + strand, within GFF2597 at 3.248 kb on + strand, within GFF2597 at 3.248 kb on + strand, within GFF2597 at 3.249 kb on - strand, within GFF2597 at 3.386 kb on + strand, within GFF2597 at 3.386 kb on + strand, within GFF2597 at 3.387 kb on - strand, within GFF2597 at 3.387 kb on - strand, within GFF2597 at 3.551 kb on + strand at 3.551 kb on + strand at 3.551 kb on + strand at 3.551 kb on + strand at 3.552 kb on - strand at 3.552 kb on - strand at 3.552 kb on - strand at 4.022 kb on - strand, within GFF2598 at 4.022 kb on - strand, within GFF2598 at 4.022 kb on - strand, within GFF2598 at 4.022 kb on - strand, within GFF2598 at 4.022 kb on - strand, within GFF2598
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 1,048 + +0.1 1,048 + +0.2 1,048 + +0.9 1,075 + -0.8 1,075 + -1.9 1,088 - +0.0 1,088 - +0.7 1,139 - -0.0 1,139 - +0.3 1,144 + -0.9 1,145 - +0.1 1,411 + GFF2595 0.32 +0.4 1,411 + GFF2595 0.32 -0.6 1,411 + GFF2595 0.32 -1.8 1,411 + GFF2595 0.32 -0.2 1,411 + GFF2595 0.32 -0.9 1,411 + GFF2595 0.32 -0.4 1,411 + GFF2595 0.32 +0.7 1,412 - GFF2595 0.32 -0.2 1,412 - GFF2595 0.32 -0.1 1,412 - GFF2595 0.32 +0.1 1,412 - GFF2595 0.32 -0.7 1,588 + GFF2595 0.55 -0.2 1,588 + GFF2595 0.55 -1.0 1,805 - GFF2595 0.83 -0.1 2,043 + -0.9 2,043 + +1.3 2,043 + +1.1 2,043 + +1.7 2,043 + -1.1 2,044 - -1.1 2,044 - -0.8 2,049 + -0.3 2,049 + +1.7 2,049 + -0.2 2,208 + GFF2596 0.23 +0.3 2,208 + GFF2596 0.23 +1.7 2,208 + GFF2596 0.23 +0.7 2,208 + GFF2596 0.23 -0.2 2,208 + GFF2596 0.23 -1.3 2,208 + GFF2596 0.23 +0.3 2,209 - GFF2596 0.23 +0.1 2,209 - GFF2596 0.23 +0.1 2,209 - GFF2596 0.23 -0.4 2,209 - GFF2596 0.23 -0.6 2,209 - GFF2596 0.23 +1.2 2,209 - GFF2596 0.23 +0.1 2,209 - GFF2596 0.23 +1.1 2,332 - GFF2596 0.33 +0.7 2,332 - GFF2596 0.33 -0.4 2,373 + GFF2596 0.36 -2.6 2,373 + GFF2596 0.36 +0.4 2,373 + GFF2596 0.36 -0.8 2,373 + GFF2596 0.36 +0.6 2,373 + GFF2596 0.36 -0.2 2,374 - GFF2596 0.36 -0.1 2,374 - GFF2596 0.36 -0.3 2,430 + GFF2596 0.41 -0.1 2,439 + GFF2596 0.42 +0.9 2,439 + GFF2596 0.42 -1.1 2,439 + GFF2596 0.42 -0.2 2,439 + GFF2596 0.42 -0.8 2,440 - GFF2596 0.42 -0.1 2,440 - GFF2596 0.42 -0.9 2,440 - GFF2596 0.42 +1.1 2,440 - GFF2596 0.42 -0.6 2,440 - GFF2596 0.42 +0.2 2,440 - GFF2596 0.42 -0.1 2,440 - GFF2596 0.42 +1.4 2,440 - GFF2596 0.42 -0.5 2,481 + GFF2596 0.45 -0.1 2,481 + GFF2596 0.45 +1.4 2,482 - GFF2596 0.45 -0.3 2,482 - GFF2596 0.45 -2.2 2,604 + GFF2596 0.55 -0.4 2,604 + GFF2596 0.55 -0.4 2,605 - GFF2596 0.55 +1.7 2,634 + GFF2596 0.58 -1.3 2,634 + GFF2596 0.58 +0.7 2,635 - GFF2596 0.58 -0.1 2,635 - GFF2596 0.58 +0.1 2,635 - GFF2596 0.58 -0.0 2,635 - GFF2596 0.58 +0.5 2,898 + GFF2596 0.79 -0.2 2,898 + GFF2596 0.79 -1.1 2,898 + GFF2596 0.79 -0.4 2,898 + GFF2596 0.79 -0.6 2,898 + GFF2596 0.79 -0.2 2,898 + GFF2596 0.79 +0.9 2,898 + GFF2596 0.79 +0.6 2,899 - GFF2596 0.79 +0.5 2,899 - GFF2596 0.79 +1.1 2,899 - GFF2596 0.79 -1.4 2,899 - GFF2596 0.79 -1.8 2,899 - GFF2596 0.79 -0.6 2,899 - GFF2596 0.79 -2.1 2,899 - GFF2596 0.79 -0.0 2,899 - GFF2596 0.79 +0.3 2,899 - GFF2596 0.79 +0.7 2,899 - GFF2596 0.79 -0.3 2,899 - GFF2596 0.79 -0.4 3,054 + -0.6 3,054 + +0.2 3,054 + +0.1 3,054 + -1.5 3,054 + -1.6 3,055 - -0.5 3,055 - +0.7 3,055 - +2.4 3,055 - +0.3 3,055 - -0.1 3,055 - +0.5 3,055 - -0.2 3,248 + GFF2597 0.23 -0.4 3,248 + GFF2597 0.23 -1.5 3,248 + GFF2597 0.23 +3.0 3,249 - GFF2597 0.23 +0.1 3,386 + GFF2597 0.55 -0.1 3,386 + GFF2597 0.55 -0.7 3,387 - GFF2597 0.55 +0.1 3,387 - GFF2597 0.55 -0.1 3,551 + -0.6 3,551 + +0.1 3,551 + -0.4 3,551 + -1.1 3,552 - -0.9 3,552 - -0.1 3,552 - +0.0 4,022 - GFF2598 0.36 -1.8 4,022 - GFF2598 0.36 -0.6 4,022 - GFF2598 0.36 -2.0 4,022 - GFF2598 0.36 -0.9 4,022 - GFF2598 0.36 +0.1
Or see this region's nucleotide sequence