Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2244 and GFF2245 overlap by 4 nucleotides GFF2245 and GFF2246 are separated by 36 nucleotides GFF2246 and GFF2247 are separated by 10 nucleotides GFF2247 and GFF2248 are separated by 35 nucleotides
GFF2244 - hypothetical protein, at 301,784 to 302,647
GFF2244
GFF2245 - Glycerol-3-phosphate ABC transporter, permease protein UgpE (TC 3.A.1.1.3), at 302,644 to 303,219
GFF2245
GFF2246 - cellular communication/signal transduction, at 303,256 to 303,612
GFF2246
GFF2247 - hypothetical protein, at 303,623 to 304,150
GFF2247
GFF2248 - Transcriptional regulatory protein RtcR, at 304,186 to 305,808
GFF2248
Position (kb) 303
304
305 Strain fitness (log2 ratio) -1
0
1 at 302.885 kb on + strand, within GFF2245 at 302.886 kb on - strand, within GFF2245 at 302.891 kb on + strand, within GFF2245 at 302.891 kb on + strand, within GFF2245 at 302.969 kb on + strand, within GFF2245 at 302.970 kb on - strand, within GFF2245 at 302.970 kb on - strand, within GFF2245 at 303.132 kb on - strand, within GFF2245 at 303.203 kb on - strand at 303.203 kb on - strand at 303.206 kb on + strand at 303.206 kb on + strand at 303.206 kb on + strand at 303.320 kb on + strand, within GFF2246 at 303.320 kb on + strand, within GFF2246 at 303.321 kb on - strand, within GFF2246 at 303.321 kb on - strand, within GFF2246 at 303.326 kb on + strand, within GFF2246 at 303.326 kb on + strand, within GFF2246 at 303.327 kb on - strand, within GFF2246 at 303.716 kb on + strand, within GFF2247 at 303.717 kb on - strand, within GFF2247 at 304.162 kb on + strand at 304.241 kb on + strand at 304.241 kb on + strand at 304.625 kb on + strand, within GFF2248 at 304.748 kb on + strand, within GFF2248 at 304.748 kb on + strand, within GFF2248 at 304.749 kb on - strand, within GFF2248 at 304.749 kb on - strand, within GFF2248 at 304.884 kb on - strand, within GFF2248
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 302,885 + GFF2245 0.42 +0.4 302,886 - GFF2245 0.42 -0.9 302,891 + GFF2245 0.43 -1.0 302,891 + GFF2245 0.43 -0.9 302,969 + GFF2245 0.56 +1.0 302,970 - GFF2245 0.57 -0.3 302,970 - GFF2245 0.57 -0.9 303,132 - GFF2245 0.85 -0.9 303,203 - +0.1 303,203 - +0.5 303,206 + +0.7 303,206 + +0.1 303,206 + -1.0 303,320 + GFF2246 0.18 +0.2 303,320 + GFF2246 0.18 +0.0 303,321 - GFF2246 0.18 -0.3 303,321 - GFF2246 0.18 +0.1 303,326 + GFF2246 0.20 -1.4 303,326 + GFF2246 0.20 -0.0 303,327 - GFF2246 0.20 -0.2 303,716 + GFF2247 0.18 +0.2 303,717 - GFF2247 0.18 +0.3 304,162 + +0.8 304,241 + -0.6 304,241 + -1.1 304,625 + GFF2248 0.27 -0.8 304,748 + GFF2248 0.35 +0.4 304,748 + GFF2248 0.35 +0.7 304,749 - GFF2248 0.35 -0.2 304,749 - GFF2248 0.35 -0.8 304,884 - GFF2248 0.43 +1.1
Or see this region's nucleotide sequence