Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2116 and GFF2117 are separated by 223 nucleotides GFF2117 and GFF2118 overlap by 4 nucleotides GFF2118 and GFF2119 overlap by 4 nucleotides GFF2119 and GFF2120 overlap by 4 nucleotides
GFF2116 - Cytochrome P450, at 165,737 to 167,131
GFF2116
GFF2117 - KEGG: type IV fimbrial biogenesis protein FimT, at 167,355 to 167,906
GFF2117
GFF2118 - hypothetical protein, at 167,903 to 168,460
GFF2118
GFF2119 - Type IV fimbrial biogenesis protein PilW, at 168,457 to 169,221
GFF2119
GFF2120 - Type IV fimbrial biogenesis protein PilX, at 169,218 to 169,799
GFF2120
Position (kb) 167
168
169 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 166.925 kb on + strand, within GFF2116 at 166.925 kb on + strand, within GFF2116 at 166.925 kb on + strand, within GFF2116 at 166.925 kb on + strand, within GFF2116 at 166.925 kb on + strand, within GFF2116 at 166.925 kb on + strand, within GFF2116 at 166.925 kb on + strand, within GFF2116 at 166.925 kb on + strand, within GFF2116 at 166.925 kb on + strand, within GFF2116 at 166.925 kb on + strand, within GFF2116 at 166.926 kb on - strand, within GFF2116 at 166.926 kb on - strand, within GFF2116 at 166.927 kb on + strand, within GFF2116 at 166.927 kb on + strand, within GFF2116 at 166.927 kb on + strand, within GFF2116 at 166.928 kb on - strand, within GFF2116 at 166.953 kb on + strand, within GFF2116 at 166.953 kb on + strand, within GFF2116 at 166.953 kb on + strand, within GFF2116 at 166.953 kb on + strand, within GFF2116 at 166.953 kb on + strand, within GFF2116 at 166.954 kb on - strand, within GFF2116 at 167.254 kb on + strand at 167.274 kb on + strand at 167.464 kb on + strand, within GFF2117 at 167.464 kb on + strand, within GFF2117 at 167.464 kb on + strand, within GFF2117 at 167.497 kb on - strand, within GFF2117 at 167.517 kb on + strand, within GFF2117 at 167.742 kb on + strand, within GFF2117 at 167.742 kb on + strand, within GFF2117 at 167.743 kb on - strand, within GFF2117 at 167.743 kb on - strand, within GFF2117 at 167.743 kb on - strand, within GFF2117 at 167.743 kb on - strand, within GFF2117 at 168.028 kb on + strand, within GFF2118 at 168.245 kb on + strand, within GFF2118 at 168.290 kb on - strand, within GFF2118 at 168.293 kb on + strand, within GFF2118 at 168.371 kb on + strand, within GFF2118 at 168.372 kb on - strand, within GFF2118 at 168.447 kb on - strand at 168.829 kb on + strand, within GFF2119 at 168.829 kb on + strand, within GFF2119 at 168.915 kb on + strand, within GFF2119 at 168.916 kb on - strand, within GFF2119 at 168.916 kb on - strand, within GFF2119 at 168.916 kb on - strand, within GFF2119 at 169.027 kb on + strand, within GFF2119 at 169.027 kb on + strand, within GFF2119 at 169.027 kb on + strand, within GFF2119 at 169.028 kb on - strand, within GFF2119 at 169.028 kb on - strand, within GFF2119 at 169.028 kb on - strand, within GFF2119 at 169.028 kb on - strand, within GFF2119 at 169.028 kb on - strand, within GFF2119 at 169.028 kb on - strand, within GFF2119 at 169.028 kb on - strand, within GFF2119 at 169.235 kb on + strand at 169.338 kb on + strand, within GFF2120 at 169.339 kb on - strand, within GFF2120 at 169.339 kb on - strand, within GFF2120 at 169.339 kb on - strand, within GFF2120 at 169.339 kb on - strand, within GFF2120
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 166,925 + GFF2116 0.85 +1.0 166,925 + GFF2116 0.85 -0.3 166,925 + GFF2116 0.85 +0.0 166,925 + GFF2116 0.85 -0.2 166,925 + GFF2116 0.85 -0.5 166,925 + GFF2116 0.85 -0.2 166,925 + GFF2116 0.85 -1.7 166,925 + GFF2116 0.85 -0.5 166,925 + GFF2116 0.85 +0.6 166,925 + GFF2116 0.85 -0.0 166,926 - GFF2116 0.85 +0.6 166,926 - GFF2116 0.85 +0.4 166,927 + GFF2116 0.85 -0.2 166,927 + GFF2116 0.85 +0.3 166,927 + GFF2116 0.85 -1.6 166,928 - GFF2116 0.85 -1.3 166,953 + GFF2116 0.87 +0.3 166,953 + GFF2116 0.87 -2.8 166,953 + GFF2116 0.87 -0.8 166,953 + GFF2116 0.87 -0.7 166,953 + GFF2116 0.87 +0.8 166,954 - GFF2116 0.87 +0.6 167,254 + -0.8 167,274 + +1.6 167,464 + GFF2117 0.20 -0.9 167,464 + GFF2117 0.20 +1.4 167,464 + GFF2117 0.20 -0.4 167,497 - GFF2117 0.26 -0.5 167,517 + GFF2117 0.29 +0.5 167,742 + GFF2117 0.70 +0.6 167,742 + GFF2117 0.70 +0.3 167,743 - GFF2117 0.70 +1.8 167,743 - GFF2117 0.70 +1.7 167,743 - GFF2117 0.70 +0.4 167,743 - GFF2117 0.70 -0.2 168,028 + GFF2118 0.22 +0.1 168,245 + GFF2118 0.61 -0.5 168,290 - GFF2118 0.69 -0.3 168,293 + GFF2118 0.70 -0.5 168,371 + GFF2118 0.84 -1.4 168,372 - GFF2118 0.84 -0.7 168,447 - -0.1 168,829 + GFF2119 0.49 +0.3 168,829 + GFF2119 0.49 +0.8 168,915 + GFF2119 0.60 -0.4 168,916 - GFF2119 0.60 -0.4 168,916 - GFF2119 0.60 +0.3 168,916 - GFF2119 0.60 -0.3 169,027 + GFF2119 0.75 -0.1 169,027 + GFF2119 0.75 -0.6 169,027 + GFF2119 0.75 -0.6 169,028 - GFF2119 0.75 -0.6 169,028 - GFF2119 0.75 -0.5 169,028 - GFF2119 0.75 +0.4 169,028 - GFF2119 0.75 -0.7 169,028 - GFF2119 0.75 -0.7 169,028 - GFF2119 0.75 -0.6 169,028 - GFF2119 0.75 -0.6 169,235 + +0.5 169,338 + GFF2120 0.21 +0.3 169,339 - GFF2120 0.21 -0.1 169,339 - GFF2120 0.21 -0.5 169,339 - GFF2120 0.21 -0.5 169,339 - GFF2120 0.21 +0.8
Or see this region's nucleotide sequence