Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2073 and GFF2074 overlap by 4 nucleotides GFF2074 and GFF2075 are separated by 4 nucleotides GFF2075 and GFF2076 are separated by 24 nucleotides
GFF2073 - Holliday junction ATP-dependent DNA helicase RuvA (EC 3.6.4.12), at 119,705 to 120,277
GFF2073
GFF2074 - hypothetical protein, at 120,274 to 121,161
GFF2074
GFF2075 - BUG/TctC family periplasmic protein, at 121,166 to 122,161
GFF2075
GFF2076 - Alkyl sulfatase and related hydrolases, MBL-fold metallo-hydrolase superfamily, at 122,186 to 123,457
GFF2076
Position (kb)
121
122
123 Strain fitness (log2 ratio)
-1
0
1
2 at 120.388 kb on + strand, within GFF2074 at 120.409 kb on + strand, within GFF2074 at 120.410 kb on - strand, within GFF2074 at 120.410 kb on - strand, within GFF2074 at 121.054 kb on + strand, within GFF2074 at 121.055 kb on - strand, within GFF2074 at 121.395 kb on + strand, within GFF2075 at 121.395 kb on + strand, within GFF2075 at 121.395 kb on + strand, within GFF2075 at 121.395 kb on + strand, within GFF2075 at 121.395 kb on + strand, within GFF2075 at 121.396 kb on - strand, within GFF2075 at 121.396 kb on - strand, within GFF2075 at 121.396 kb on - strand, within GFF2075 at 121.396 kb on - strand, within GFF2075 at 121.587 kb on + strand, within GFF2075 at 121.587 kb on + strand, within GFF2075 at 121.588 kb on - strand, within GFF2075 at 121.674 kb on + strand, within GFF2075 at 121.674 kb on + strand, within GFF2075 at 121.674 kb on + strand, within GFF2075 at 121.674 kb on + strand, within GFF2075 at 121.674 kb on + strand, within GFF2075 at 121.675 kb on - strand, within GFF2075 at 121.675 kb on - strand, within GFF2075 at 121.675 kb on - strand, within GFF2075 at 121.675 kb on - strand, within GFF2075 at 121.675 kb on - strand, within GFF2075 at 121.675 kb on - strand, within GFF2075 at 121.704 kb on + strand, within GFF2075 at 121.827 kb on + strand, within GFF2075 at 121.828 kb on - strand, within GFF2075 at 121.828 kb on - strand, within GFF2075 at 121.860 kb on + strand, within GFF2075 at 121.860 kb on + strand, within GFF2075 at 121.860 kb on + strand, within GFF2075 at 121.860 kb on + strand, within GFF2075 at 121.860 kb on + strand, within GFF2075 at 122.061 kb on + strand, within GFF2075 at 122.061 kb on + strand, within GFF2075 at 122.062 kb on - strand at 122.062 kb on - strand at 122.244 kb on + strand at 122.245 kb on - strand at 122.263 kb on - strand at 122.263 kb on - strand at 122.263 kb on - strand at 122.263 kb on - strand at 122.263 kb on - strand at 122.263 kb on - strand at 122.532 kb on + strand, within GFF2076 at 122.532 kb on + strand, within GFF2076 at 122.532 kb on + strand, within GFF2076 at 122.532 kb on + strand, within GFF2076 at 122.533 kb on - strand, within GFF2076 at 122.533 kb on - strand, within GFF2076 at 122.533 kb on - strand, within GFF2076 at 122.550 kb on + strand, within GFF2076 at 122.550 kb on + strand, within GFF2076 at 122.550 kb on + strand, within GFF2076 at 122.565 kb on + strand, within GFF2076 at 122.566 kb on - strand, within GFF2076 at 122.971 kb on + strand, within GFF2076 at 122.972 kb on + strand, within GFF2076 at 122.973 kb on - strand, within GFF2076 at 122.973 kb on - strand, within GFF2076 at 122.973 kb on - strand, within GFF2076
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 120,388 + GFF2074 0.13 +0.0 120,409 + GFF2074 0.15 +0.8 120,410 - GFF2074 0.15 -0.4 120,410 - GFF2074 0.15 +0.2 121,054 + GFF2074 0.88 +0.3 121,055 - GFF2074 0.88 -0.5 121,395 + GFF2075 0.23 -1.1 121,395 + GFF2075 0.23 +0.1 121,395 + GFF2075 0.23 +0.3 121,395 + GFF2075 0.23 +1.1 121,395 + GFF2075 0.23 -0.2 121,396 - GFF2075 0.23 +1.6 121,396 - GFF2075 0.23 -0.7 121,396 - GFF2075 0.23 -0.1 121,396 - GFF2075 0.23 -1.0 121,587 + GFF2075 0.42 -0.1 121,587 + GFF2075 0.42 +0.0 121,588 - GFF2075 0.42 +0.1 121,674 + GFF2075 0.51 -1.1 121,674 + GFF2075 0.51 +0.1 121,674 + GFF2075 0.51 -0.2 121,674 + GFF2075 0.51 +1.2 121,674 + GFF2075 0.51 -0.6 121,675 - GFF2075 0.51 +0.2 121,675 - GFF2075 0.51 +0.5 121,675 - GFF2075 0.51 -0.4 121,675 - GFF2075 0.51 +0.3 121,675 - GFF2075 0.51 +1.4 121,675 - GFF2075 0.51 -0.3 121,704 + GFF2075 0.54 +0.5 121,827 + GFF2075 0.66 -0.0 121,828 - GFF2075 0.66 -0.4 121,828 - GFF2075 0.66 +0.3 121,860 + GFF2075 0.70 -1.0 121,860 + GFF2075 0.70 -0.8 121,860 + GFF2075 0.70 +0.2 121,860 + GFF2075 0.70 -1.2 121,860 + GFF2075 0.70 -0.7 122,061 + GFF2075 0.90 -0.3 122,061 + GFF2075 0.90 -0.8 122,062 - -1.1 122,062 - +0.1 122,244 + -0.4 122,245 - -0.7 122,263 - -0.3 122,263 - -1.0 122,263 - -1.3 122,263 - -1.3 122,263 - -0.1 122,263 - -0.2 122,532 + GFF2076 0.27 -0.6 122,532 + GFF2076 0.27 -0.0 122,532 + GFF2076 0.27 +1.0 122,532 + GFF2076 0.27 -0.7 122,533 - GFF2076 0.27 -0.6 122,533 - GFF2076 0.27 +0.4 122,533 - GFF2076 0.27 +0.0 122,550 + GFF2076 0.29 -1.1 122,550 + GFF2076 0.29 +0.3 122,550 + GFF2076 0.29 -0.6 122,565 + GFF2076 0.30 +0.7 122,566 - GFF2076 0.30 +1.8 122,971 + GFF2076 0.62 +0.1 122,972 + GFF2076 0.62 -1.3 122,973 - GFF2076 0.62 +0.1 122,973 - GFF2076 0.62 -1.1 122,973 - GFF2076 0.62 -0.2
Or see this region's nucleotide sequence