Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1887 and GFF1888 are separated by 4 nucleotides GFF1888 and GFF1889 are separated by 30 nucleotides
GFF1887 - Fumarate hydratase class II (EC 4.2.1.2), at 562,656 to 564,038
GFF1887
GFF1888 - BUG/TctC family periplasmic protein, at 564,043 to 565,032
GFF1888
GFF1889 - Aerobic C4-dicarboxylate transporter for fumarate, L-malate, D-malate, succunate, at 565,063 to 566,451
GFF1889
Position (kb) 564
565
566 Strain fitness (log2 ratio) -2
-1
0
1
2 at 563.943 kb on + strand at 563.943 kb on + strand at 563.977 kb on + strand at 564.132 kb on - strand at 564.132 kb on - strand at 564.154 kb on + strand, within GFF1888 at 564.154 kb on + strand, within GFF1888 at 564.154 kb on + strand, within GFF1888 at 564.154 kb on + strand, within GFF1888 at 564.155 kb on - strand, within GFF1888 at 564.209 kb on + strand, within GFF1888 at 564.209 kb on + strand, within GFF1888 at 564.209 kb on + strand, within GFF1888 at 564.260 kb on + strand, within GFF1888 at 564.261 kb on - strand, within GFF1888 at 564.473 kb on + strand, within GFF1888 at 564.473 kb on + strand, within GFF1888 at 564.473 kb on + strand, within GFF1888 at 564.474 kb on - strand, within GFF1888 at 564.699 kb on - strand, within GFF1888 at 564.714 kb on - strand, within GFF1888 at 565.479 kb on - strand, within GFF1889 at 565.757 kb on + strand, within GFF1889 at 565.758 kb on - strand, within GFF1889 at 565.758 kb on - strand, within GFF1889 at 565.758 kb on - strand, within GFF1889 at 565.758 kb on - strand, within GFF1889 at 565.758 kb on - strand, within GFF1889
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 563,943 + -0.4 563,943 + -0.2 563,977 + -0.5 564,132 - -1.5 564,132 - +0.1 564,154 + GFF1888 0.11 -2.2 564,154 + GFF1888 0.11 -0.1 564,154 + GFF1888 0.11 +1.3 564,154 + GFF1888 0.11 +2.6 564,155 - GFF1888 0.11 -0.1 564,209 + GFF1888 0.17 -0.3 564,209 + GFF1888 0.17 +0.8 564,209 + GFF1888 0.17 +0.2 564,260 + GFF1888 0.22 +0.5 564,261 - GFF1888 0.22 -0.2 564,473 + GFF1888 0.43 -1.5 564,473 + GFF1888 0.43 -0.7 564,473 + GFF1888 0.43 +1.4 564,474 - GFF1888 0.44 -0.3 564,699 - GFF1888 0.66 +0.8 564,714 - GFF1888 0.68 +0.7 565,479 - GFF1889 0.30 +0.6 565,757 + GFF1889 0.50 -0.9 565,758 - GFF1889 0.50 -0.5 565,758 - GFF1889 0.50 -0.3 565,758 - GFF1889 0.50 +0.3 565,758 - GFF1889 0.50 -0.2 565,758 - GFF1889 0.50 +0.1
Or see this region's nucleotide sequence