Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1875 and GFF1874 overlap by 2 nucleotides GFF1874 and GFF1876 are separated by 12 nucleotides GFF1876 and GFF1877 are separated by 9 nucleotides GFF1877 and GFF1878 are separated by 33 nucleotides
GFF1875 - no description, at 548,492 to 548,626
GFF1875
GFF1874 - 4-hydroxy-tetrahydrodipicolinate synthase (EC 4.3.3.7), at 548,625 to 549,581
GFF1874
GFF1876 - Dihydrodipicolinate synthase family, at 549,594 to 550,487
GFF1876
GFF1877 - ABC transporter, ATP-binding protein (cluster 3, basic aa/glutamine/opines), at 550,497 to 551,279
GFF1877
GFF1878 - Bll6165 protein, at 551,313 to 551,723
GFF1878
Position (kb) 549
550
551 Strain fitness (log2 ratio) -2
-1
0
1
2 at 549.249 kb on + strand, within GFF1874 at 549.250 kb on - strand, within GFF1874 at 549.390 kb on + strand, within GFF1874 at 549.390 kb on + strand, within GFF1874 at 549.402 kb on + strand, within GFF1874 at 549.402 kb on + strand, within GFF1874 at 549.403 kb on - strand, within GFF1874 at 549.615 kb on + strand at 549.615 kb on + strand at 549.615 kb on + strand at 549.615 kb on + strand at 549.615 kb on + strand at 549.616 kb on - strand at 549.616 kb on - strand at 549.678 kb on + strand at 549.678 kb on + strand at 549.678 kb on + strand at 549.678 kb on + strand at 549.918 kb on + strand, within GFF1876 at 549.919 kb on - strand, within GFF1876 at 549.919 kb on - strand, within GFF1876 at 549.954 kb on + strand, within GFF1876 at 549.954 kb on + strand, within GFF1876 at 549.955 kb on - strand, within GFF1876 at 549.955 kb on - strand, within GFF1876 at 549.996 kb on + strand, within GFF1876 at 549.996 kb on + strand, within GFF1876 at 549.996 kb on + strand, within GFF1876 at 549.996 kb on + strand, within GFF1876 at 549.997 kb on - strand, within GFF1876 at 549.997 kb on - strand, within GFF1876 at 550.074 kb on + strand, within GFF1876 at 550.075 kb on - strand, within GFF1876 at 550.075 kb on - strand, within GFF1876 at 550.075 kb on - strand, within GFF1876 at 550.176 kb on + strand, within GFF1876 at 550.176 kb on + strand, within GFF1876 at 550.177 kb on - strand, within GFF1876 at 550.177 kb on - strand, within GFF1876 at 550.177 kb on - strand, within GFF1876 at 550.177 kb on - strand, within GFF1876 at 550.177 kb on - strand, within GFF1876 at 550.245 kb on + strand, within GFF1876 at 550.246 kb on - strand, within GFF1876 at 550.246 kb on - strand, within GFF1876 at 550.335 kb on + strand, within GFF1876 at 550.335 kb on + strand, within GFF1876 at 550.335 kb on + strand, within GFF1876 at 550.335 kb on + strand, within GFF1876 at 550.336 kb on - strand, within GFF1876 at 550.336 kb on - strand, within GFF1876 at 550.336 kb on - strand, within GFF1876 at 550.336 kb on - strand, within GFF1876 at 550.490 kb on + strand at 550.490 kb on + strand at 550.490 kb on + strand at 550.491 kb on - strand at 550.951 kb on - strand, within GFF1877 at 551.287 kb on + strand at 551.287 kb on + strand at 551.295 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 549,249 + GFF1874 0.65 +0.1 549,250 - GFF1874 0.65 +0.3 549,390 + GFF1874 0.80 +0.2 549,390 + GFF1874 0.80 -1.3 549,402 + GFF1874 0.81 -1.0 549,402 + GFF1874 0.81 -0.2 549,403 - GFF1874 0.81 -0.4 549,615 + -0.7 549,615 + -0.4 549,615 + +0.5 549,615 + +1.6 549,615 + -0.7 549,616 - +0.1 549,616 - -0.2 549,678 + -1.0 549,678 + -0.7 549,678 + +0.2 549,678 + -0.4 549,918 + GFF1876 0.36 +0.1 549,919 - GFF1876 0.36 +0.4 549,919 - GFF1876 0.36 -1.2 549,954 + GFF1876 0.40 -0.3 549,954 + GFF1876 0.40 -0.2 549,955 - GFF1876 0.40 +0.4 549,955 - GFF1876 0.40 -1.4 549,996 + GFF1876 0.45 +1.8 549,996 + GFF1876 0.45 -0.3 549,996 + GFF1876 0.45 +0.4 549,996 + GFF1876 0.45 -0.4 549,997 - GFF1876 0.45 +0.2 549,997 - GFF1876 0.45 -1.0 550,074 + GFF1876 0.54 +0.4 550,075 - GFF1876 0.54 +1.6 550,075 - GFF1876 0.54 -0.2 550,075 - GFF1876 0.54 +0.0 550,176 + GFF1876 0.65 -0.5 550,176 + GFF1876 0.65 +2.3 550,177 - GFF1876 0.65 -0.0 550,177 - GFF1876 0.65 -0.1 550,177 - GFF1876 0.65 +0.6 550,177 - GFF1876 0.65 -1.9 550,177 - GFF1876 0.65 +1.8 550,245 + GFF1876 0.73 +0.4 550,246 - GFF1876 0.73 -1.2 550,246 - GFF1876 0.73 -0.2 550,335 + GFF1876 0.83 +0.0 550,335 + GFF1876 0.83 -0.4 550,335 + GFF1876 0.83 +1.1 550,335 + GFF1876 0.83 -0.6 550,336 - GFF1876 0.83 -0.1 550,336 - GFF1876 0.83 -0.6 550,336 - GFF1876 0.83 -0.3 550,336 - GFF1876 0.83 -0.4 550,490 + -0.2 550,490 + -1.5 550,490 + -0.1 550,491 - -1.1 550,951 - GFF1877 0.58 +1.0 551,287 + +1.1 551,287 + +1.0 551,295 - -1.1
Or see this region's nucleotide sequence