Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1828 and GFF1829 are separated by 112 nucleotides GFF1829 and GFF1830 overlap by 1 nucleotides GFF1830 and GFF1831 overlap by 4 nucleotides GFF1831 and GFF1832 are separated by 80 nucleotides
GFF1828 - phosphodiesterase I, at 493,174 to 495,015
GFF1828
GFF1829 - 4-hydroxybenzoyl-CoA thioesterase (EC 3.1.2.23), at 495,128 to 495,556
GFF1829
GFF1830 - TRAP dicarboxylate transporter, DctM subunit, unknown substrate 3, at 495,556 to 496,857
GFF1830
GFF1831 - TRAP dicarboxylate transporter, DctQ subunit, unknown substrate 3, at 496,854 to 497,354
GFF1831
GFF1832 - TRAP transporter solute receptor, unknown substrate 3, at 497,435 to 498,490
GFF1832
Position (kb) 495
496
497 Strain fitness (log2 ratio) -1
0
1 at 494.847 kb on - strand at 494.904 kb on + strand at 494.905 kb on - strand at 494.979 kb on - strand at 494.979 kb on - strand at 495.256 kb on - strand, within GFF1829 at 495.322 kb on - strand, within GFF1829 at 495.869 kb on + strand, within GFF1830 at 496.286 kb on + strand, within GFF1830 at 496.745 kb on + strand at 496.751 kb on + strand at 496.806 kb on + strand at 496.810 kb on + strand at 496.811 kb on - strand at 496.811 kb on - strand at 496.811 kb on - strand at 496.975 kb on + strand, within GFF1831 at 496.975 kb on + strand, within GFF1831 at 496.976 kb on - strand, within GFF1831 at 496.976 kb on - strand, within GFF1831 at 497.005 kb on + strand, within GFF1831 at 497.173 kb on + strand, within GFF1831 at 497.357 kb on - strand at 497.451 kb on + strand at 497.589 kb on + strand, within GFF1832 at 497.590 kb on - strand, within GFF1832 at 497.730 kb on + strand, within GFF1832 at 497.731 kb on - strand, within GFF1832 at 497.731 kb on - strand, within GFF1832 at 497.731 kb on - strand, within GFF1832 at 497.769 kb on + strand, within GFF1832 at 497.769 kb on + strand, within GFF1832 at 497.770 kb on - strand, within GFF1832 at 497.770 kb on - strand, within GFF1832 at 497.791 kb on - strand, within GFF1832
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 494,847 - -0.1 494,904 + -0.8 494,905 - +0.5 494,979 - -1.0 494,979 - -1.0 495,256 - GFF1829 0.30 +1.5 495,322 - GFF1829 0.45 -0.6 495,869 + GFF1830 0.24 -1.0 496,286 + GFF1830 0.56 +0.0 496,745 + +0.5 496,751 + -0.0 496,806 + -0.4 496,810 + +0.2 496,811 - -1.0 496,811 - -0.2 496,811 - +0.8 496,975 + GFF1831 0.24 -1.3 496,975 + GFF1831 0.24 +0.6 496,976 - GFF1831 0.24 +0.7 496,976 - GFF1831 0.24 -0.5 497,005 + GFF1831 0.30 +0.8 497,173 + GFF1831 0.64 +0.3 497,357 - -0.2 497,451 + +0.7 497,589 + GFF1832 0.15 +0.8 497,590 - GFF1832 0.15 +0.5 497,730 + GFF1832 0.28 -1.0 497,731 - GFF1832 0.28 +1.3 497,731 - GFF1832 0.28 +1.5 497,731 - GFF1832 0.28 -0.3 497,769 + GFF1832 0.32 -1.2 497,769 + GFF1832 0.32 +0.1 497,770 - GFF1832 0.32 +0.3 497,770 - GFF1832 0.32 -0.2 497,791 - GFF1832 0.34 -0.7
Or see this region's nucleotide sequence