Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1736 and GFF1737 are separated by 212 nucleotides GFF1737 and GFF1738 are separated by 4 nucleotides GFF1738 and GFF1739 are separated by 128 nucleotides
GFF1736 - 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases, at 394,721 to 396,010
GFF1736
GFF1737 - Carbon starvation protein A, at 396,223 to 398,289
GFF1737
GFF1738 - Uncharacterized proteins YbdD and YjiX, at 398,294 to 398,500
GFF1738
GFF1739 - Glutamate carboxypeptidase (EC 3.4.17.11), at 398,629 to 399,906
GFF1739
Position (kb) 396
397
398
399 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 395.304 kb on + strand, within GFF1736 at 395.305 kb on - strand, within GFF1736 at 395.305 kb on - strand, within GFF1736 at 395.421 kb on + strand, within GFF1736 at 395.422 kb on - strand, within GFF1736 at 395.810 kb on + strand, within GFF1736 at 396.042 kb on + strand at 396.042 kb on + strand at 396.043 kb on - strand at 396.043 kb on - strand at 396.043 kb on - strand at 396.043 kb on - strand at 396.073 kb on + strand at 396.073 kb on + strand at 396.355 kb on + strand at 396.355 kb on + strand at 396.355 kb on + strand at 396.355 kb on + strand at 396.355 kb on + strand at 396.356 kb on - strand at 396.356 kb on - strand at 396.368 kb on - strand at 396.373 kb on + strand at 396.373 kb on + strand at 396.376 kb on + strand at 396.376 kb on + strand at 396.376 kb on + strand at 396.376 kb on + strand at 396.376 kb on + strand at 396.377 kb on - strand at 396.461 kb on - strand, within GFF1737 at 396.559 kb on + strand, within GFF1737 at 396.560 kb on - strand, within GFF1737 at 396.847 kb on + strand, within GFF1737 at 396.847 kb on + strand, within GFF1737 at 396.847 kb on + strand, within GFF1737 at 396.848 kb on - strand, within GFF1737 at 396.848 kb on - strand, within GFF1737 at 396.848 kb on - strand, within GFF1737 at 396.856 kb on + strand, within GFF1737 at 396.857 kb on - strand, within GFF1737 at 396.857 kb on - strand, within GFF1737 at 396.857 kb on - strand, within GFF1737 at 397.010 kb on - strand, within GFF1737 at 397.063 kb on + strand, within GFF1737 at 397.063 kb on + strand, within GFF1737 at 397.390 kb on + strand, within GFF1737 at 397.391 kb on - strand, within GFF1737 at 397.391 kb on - strand, within GFF1737 at 397.391 kb on - strand, within GFF1737 at 397.621 kb on + strand, within GFF1737 at 397.621 kb on + strand, within GFF1737 at 397.668 kb on - strand, within GFF1737 at 397.807 kb on + strand, within GFF1737 at 397.808 kb on - strand, within GFF1737 at 397.940 kb on - strand, within GFF1737 at 398.057 kb on - strand, within GFF1737 at 398.194 kb on + strand at 398.194 kb on + strand at 398.195 kb on - strand at 398.324 kb on + strand, within GFF1738 at 398.379 kb on - strand, within GFF1738 at 398.388 kb on - strand, within GFF1738 at 398.435 kb on + strand, within GFF1738 at 398.498 kb on + strand at 398.499 kb on - strand at 398.499 kb on - strand at 398.559 kb on + strand at 398.559 kb on + strand at 398.559 kb on + strand at 398.560 kb on - strand at 398.560 kb on - strand at 398.560 kb on - strand at 398.785 kb on + strand, within GFF1739 at 398.786 kb on - strand, within GFF1739 at 398.786 kb on - strand, within GFF1739 at 398.786 kb on - strand, within GFF1739 at 399.049 kb on + strand, within GFF1739 at 399.049 kb on + strand, within GFF1739 at 399.050 kb on - strand, within GFF1739 at 399.050 kb on - strand, within GFF1739 at 399.050 kb on - strand, within GFF1739 at 399.050 kb on - strand, within GFF1739 at 399.119 kb on - strand, within GFF1739 at 399.119 kb on - strand, within GFF1739 at 399.157 kb on + strand, within GFF1739
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 395,304 + GFF1736 0.45 -0.3 395,305 - GFF1736 0.45 +0.8 395,305 - GFF1736 0.45 -0.4 395,421 + GFF1736 0.54 -0.2 395,422 - GFF1736 0.54 -0.6 395,810 + GFF1736 0.84 +0.3 396,042 + +0.2 396,042 + +0.3 396,043 - -0.2 396,043 - +0.2 396,043 - +0.0 396,043 - +0.3 396,073 + -0.4 396,073 + -0.9 396,355 + +1.0 396,355 + -0.1 396,355 + -1.0 396,355 + -0.3 396,355 + +0.4 396,356 - +0.6 396,356 - +0.4 396,368 - -0.4 396,373 + +0.4 396,373 + +0.1 396,376 + -0.5 396,376 + -0.4 396,376 + -0.0 396,376 + +0.5 396,376 + +0.8 396,377 - +0.0 396,461 - GFF1737 0.12 -0.2 396,559 + GFF1737 0.16 -0.3 396,560 - GFF1737 0.16 -1.6 396,847 + GFF1737 0.30 +0.5 396,847 + GFF1737 0.30 +0.8 396,847 + GFF1737 0.30 -0.2 396,848 - GFF1737 0.30 +1.2 396,848 - GFF1737 0.30 +0.8 396,848 - GFF1737 0.30 +0.0 396,856 + GFF1737 0.31 -1.0 396,857 - GFF1737 0.31 +0.1 396,857 - GFF1737 0.31 +0.5 396,857 - GFF1737 0.31 +0.6 397,010 - GFF1737 0.38 +1.3 397,063 + GFF1737 0.41 +0.6 397,063 + GFF1737 0.41 -0.5 397,390 + GFF1737 0.56 +0.2 397,391 - GFF1737 0.57 +0.0 397,391 - GFF1737 0.57 -0.2 397,391 - GFF1737 0.57 -1.0 397,621 + GFF1737 0.68 +0.3 397,621 + GFF1737 0.68 -0.0 397,668 - GFF1737 0.70 -0.7 397,807 + GFF1737 0.77 -1.7 397,808 - GFF1737 0.77 +1.1 397,940 - GFF1737 0.83 +0.7 398,057 - GFF1737 0.89 -0.2 398,194 + +0.3 398,194 + +0.1 398,195 - -0.1 398,324 + GFF1738 0.14 -0.3 398,379 - GFF1738 0.41 +0.7 398,388 - GFF1738 0.45 +0.9 398,435 + GFF1738 0.68 +0.4 398,498 + +0.3 398,499 - +0.8 398,499 - -1.8 398,559 + +0.3 398,559 + -0.5 398,559 + -1.2 398,560 - +0.6 398,560 - +0.0 398,560 - -0.1 398,785 + GFF1739 0.12 -0.4 398,786 - GFF1739 0.12 -0.2 398,786 - GFF1739 0.12 -0.3 398,786 - GFF1739 0.12 -0.4 399,049 + GFF1739 0.33 -0.2 399,049 + GFF1739 0.33 -0.4 399,050 - GFF1739 0.33 +4.2 399,050 - GFF1739 0.33 -0.1 399,050 - GFF1739 0.33 +0.0 399,050 - GFF1739 0.33 -0.7 399,119 - GFF1739 0.38 -1.6 399,119 - GFF1739 0.38 -0.3 399,157 + GFF1739 0.41 +0.3
Or see this region's nucleotide sequence