Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1535 and GFF1536 overlap by 4 nucleotides GFF1536 and GFF1537 are separated by 10 nucleotides
GFF1535 - Shikimate 5-dehydrogenase I alpha (EC 1.1.1.25), at 133,012 to 133,917
GFF1535
GFF1536 - BUG/TctC family periplasmic protein, at 133,914 to 134,897
GFF1536
GFF1537 - FIG01127817: hypothetical protein, at 134,908 to 136,569
GFF1537
Position (kb) 133
134
135 Strain fitness (log2 ratio) -2
-1
0
1
2 at 132.972 kb on + strand at 132.998 kb on + strand at 132.998 kb on + strand at 132.999 kb on - strand at 132.999 kb on - strand at 132.999 kb on - strand at 132.999 kb on - strand at 132.999 kb on - strand at 133.264 kb on + strand, within GFF1535 at 133.265 kb on - strand, within GFF1535 at 133.265 kb on - strand, within GFF1535 at 133.823 kb on - strand, within GFF1535 at 134.001 kb on + strand at 134.233 kb on - strand, within GFF1536 at 134.248 kb on - strand, within GFF1536 at 134.385 kb on + strand, within GFF1536 at 134.385 kb on + strand, within GFF1536 at 134.385 kb on + strand, within GFF1536 at 134.385 kb on + strand, within GFF1536 at 134.386 kb on - strand, within GFF1536 at 134.475 kb on + strand, within GFF1536 at 134.476 kb on - strand, within GFF1536 at 134.476 kb on - strand, within GFF1536 at 134.857 kb on - strand at 135.145 kb on + strand, within GFF1537 at 135.145 kb on + strand, within GFF1537 at 135.145 kb on + strand, within GFF1537 at 135.146 kb on - strand, within GFF1537 at 135.146 kb on - strand, within GFF1537 at 135.146 kb on - strand, within GFF1537 at 135.175 kb on + strand, within GFF1537 at 135.175 kb on + strand, within GFF1537 at 135.176 kb on - strand, within GFF1537 at 135.176 kb on - strand, within GFF1537 at 135.178 kb on + strand, within GFF1537 at 135.178 kb on + strand, within GFF1537 at 135.179 kb on - strand, within GFF1537 at 135.601 kb on + strand, within GFF1537 at 135.601 kb on + strand, within GFF1537 at 135.602 kb on - strand, within GFF1537 at 135.775 kb on + strand, within GFF1537 at 135.775 kb on + strand, within GFF1537 at 135.775 kb on + strand, within GFF1537 at 135.775 kb on + strand, within GFF1537 at 135.775 kb on + strand, within GFF1537 at 135.776 kb on - strand, within GFF1537 at 135.776 kb on - strand, within GFF1537 at 135.776 kb on - strand, within GFF1537 at 135.832 kb on + strand, within GFF1537 at 135.833 kb on - strand, within GFF1537 at 135.875 kb on - strand, within GFF1537
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 132,972 + +0.2 132,998 + -0.3 132,998 + -0.4 132,999 - +0.5 132,999 - +0.1 132,999 - +0.4 132,999 - +0.1 132,999 - -2.2 133,264 + GFF1535 0.28 +1.1 133,265 - GFF1535 0.28 -1.4 133,265 - GFF1535 0.28 -1.7 133,823 - GFF1535 0.90 +0.4 134,001 + +0.8 134,233 - GFF1536 0.32 +0.4 134,248 - GFF1536 0.34 -0.2 134,385 + GFF1536 0.48 -0.2 134,385 + GFF1536 0.48 -0.2 134,385 + GFF1536 0.48 +0.7 134,385 + GFF1536 0.48 -0.8 134,386 - GFF1536 0.48 +0.1 134,475 + GFF1536 0.57 +0.5 134,476 - GFF1536 0.57 +0.5 134,476 - GFF1536 0.57 +1.2 134,857 - -1.1 135,145 + GFF1537 0.14 -0.2 135,145 + GFF1537 0.14 +2.2 135,145 + GFF1537 0.14 -0.2 135,146 - GFF1537 0.14 -2.1 135,146 - GFF1537 0.14 -0.2 135,146 - GFF1537 0.14 +0.1 135,175 + GFF1537 0.16 -0.5 135,175 + GFF1537 0.16 -0.8 135,176 - GFF1537 0.16 +0.0 135,176 - GFF1537 0.16 -0.0 135,178 + GFF1537 0.16 +0.0 135,178 + GFF1537 0.16 -0.2 135,179 - GFF1537 0.16 +0.8 135,601 + GFF1537 0.42 +0.6 135,601 + GFF1537 0.42 +0.2 135,602 - GFF1537 0.42 +0.5 135,775 + GFF1537 0.52 +0.3 135,775 + GFF1537 0.52 +0.3 135,775 + GFF1537 0.52 +0.3 135,775 + GFF1537 0.52 +0.3 135,775 + GFF1537 0.52 -0.3 135,776 - GFF1537 0.52 -2.0 135,776 - GFF1537 0.52 -1.6 135,776 - GFF1537 0.52 +0.3 135,832 + GFF1537 0.56 -0.5 135,833 - GFF1537 0.56 -0.2 135,875 - GFF1537 0.58 +1.0
Or see this region's nucleotide sequence