Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF151 and GFF152 are separated by 27 nucleotides GFF152 and GFF153 overlap by 4 nucleotides GFF153 and GFF154 overlap by 4 nucleotides
GFF151 - FIG00460174: hypothetical protein, at 178,819 to 179,706
GFF151
GFF152 - ABC transporter, permease protein 1 (cluster 5, nickel/peptides/opines), at 179,734 to 180,678
GFF152
GFF153 - ABC transporter, permease protein 2 (cluster 5, nickel/peptides/opines), at 180,675 to 181,511
GFF153
GFF154 - ABC transporter, ATP-binding protein (cluster 5, nickel/peptides/opines) / ABC transporter, ATP-binding protein (cluster 5, nickel/peptides/opines), at 181,508 to 183,178
GFF154
Position (kb) 180
181
182 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 179.726 kb on + strand at 179.727 kb on - strand at 179.727 kb on - strand at 179.813 kb on + strand at 179.814 kb on - strand at 180.232 kb on + strand, within GFF152 at 180.232 kb on + strand, within GFF152 at 180.233 kb on - strand, within GFF152 at 180.271 kb on + strand, within GFF152 at 180.272 kb on - strand, within GFF152 at 180.272 kb on - strand, within GFF152 at 180.367 kb on + strand, within GFF152 at 180.367 kb on + strand, within GFF152 at 180.367 kb on + strand, within GFF152 at 180.368 kb on - strand, within GFF152 at 180.368 kb on - strand, within GFF152 at 180.368 kb on - strand, within GFF152 at 180.368 kb on - strand, within GFF152 at 180.578 kb on + strand, within GFF152 at 180.578 kb on + strand, within GFF152 at 180.578 kb on + strand, within GFF152 at 180.579 kb on - strand, within GFF152 at 180.579 kb on - strand, within GFF152 at 180.579 kb on - strand, within GFF152 at 180.579 kb on - strand, within GFF152 at 180.610 kb on + strand at 180.610 kb on + strand at 180.610 kb on + strand at 180.610 kb on + strand at 180.610 kb on + strand at 180.611 kb on - strand at 180.611 kb on - strand at 180.611 kb on - strand at 180.611 kb on - strand at 180.643 kb on + strand at 181.116 kb on + strand, within GFF153 at 181.215 kb on + strand, within GFF153 at 181.215 kb on + strand, within GFF153 at 181.215 kb on + strand, within GFF153 at 181.216 kb on - strand, within GFF153 at 181.216 kb on - strand, within GFF153 at 181.216 kb on - strand, within GFF153 at 181.216 kb on - strand, within GFF153 at 181.401 kb on + strand, within GFF153 at 181.401 kb on + strand, within GFF153 at 181.401 kb on + strand, within GFF153 at 181.402 kb on - strand, within GFF153 at 181.402 kb on - strand, within GFF153 at 181.402 kb on - strand, within GFF153 at 181.402 kb on - strand, within GFF153 at 181.402 kb on - strand, within GFF153 at 181.402 kb on - strand, within GFF153 at 181.402 kb on - strand, within GFF153 at 182.273 kb on + strand, within GFF154 at 182.273 kb on + strand, within GFF154 at 182.273 kb on + strand, within GFF154 at 182.373 kb on - strand, within GFF154 at 182.373 kb on - strand, within GFF154 at 182.373 kb on - strand, within GFF154
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 179,726 + +0.4 179,727 - -0.1 179,727 - -0.7 179,813 + +0.8 179,814 - +0.1 180,232 + GFF152 0.53 -1.1 180,232 + GFF152 0.53 +0.4 180,233 - GFF152 0.53 -0.0 180,271 + GFF152 0.57 +0.2 180,272 - GFF152 0.57 -0.6 180,272 - GFF152 0.57 -1.2 180,367 + GFF152 0.67 +0.1 180,367 + GFF152 0.67 +2.0 180,367 + GFF152 0.67 -0.9 180,368 - GFF152 0.67 -1.2 180,368 - GFF152 0.67 -0.8 180,368 - GFF152 0.67 -0.7 180,368 - GFF152 0.67 +1.4 180,578 + GFF152 0.89 -0.1 180,578 + GFF152 0.89 -2.8 180,578 + GFF152 0.89 -1.3 180,579 - GFF152 0.89 +0.1 180,579 - GFF152 0.89 +0.0 180,579 - GFF152 0.89 -1.2 180,579 - GFF152 0.89 -0.4 180,610 + -1.2 180,610 + +0.4 180,610 + -0.6 180,610 + -0.1 180,610 + -0.9 180,611 - -0.7 180,611 - -0.0 180,611 - -1.4 180,611 - +0.8 180,643 + +0.3 181,116 + GFF153 0.53 -0.6 181,215 + GFF153 0.65 -0.5 181,215 + GFF153 0.65 -0.4 181,215 + GFF153 0.65 -2.5 181,216 - GFF153 0.65 +0.4 181,216 - GFF153 0.65 +0.6 181,216 - GFF153 0.65 +0.0 181,216 - GFF153 0.65 -0.6 181,401 + GFF153 0.87 +0.7 181,401 + GFF153 0.87 -0.8 181,401 + GFF153 0.87 -0.1 181,402 - GFF153 0.87 -0.2 181,402 - GFF153 0.87 -0.3 181,402 - GFF153 0.87 +0.1 181,402 - GFF153 0.87 -0.6 181,402 - GFF153 0.87 +2.0 181,402 - GFF153 0.87 -0.3 181,402 - GFF153 0.87 -0.4 182,273 + GFF154 0.46 -0.3 182,273 + GFF154 0.46 -0.5 182,273 + GFF154 0.46 -0.0 182,373 - GFF154 0.52 -0.4 182,373 - GFF154 0.52 +0.6 182,373 - GFF154 0.52 -0.6
Or see this region's nucleotide sequence