Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1507 and GFF1508 overlap by 4 nucleotides GFF1508 and GFF1509 overlap by 4 nucleotides GFF1509 and GFF1510 overlap by 4 nucleotides
GFF1507 - ABC transporter, ATP-binding protein (cluster 5, nickel/peptides/opines), at 99,377 to 101,443
GFF1507
GFF1508 - ABC transporter, permease protein 2 (cluster 5, nickel/peptides/opines), at 101,440 to 102,339
GFF1508
GFF1509 - ABC transporter, permease protein 1 (cluster 5, nickel/peptides/opines), at 102,336 to 103,310
GFF1509
GFF1510 - Oxidoreductase, at 103,307 to 104,797
GFF1510
Position (kb) 102
103
104 Strain fitness (log2 ratio) -2
-1
0
1
2 at 101.546 kb on + strand, within GFF1508 at 101.547 kb on - strand, within GFF1508 at 101.570 kb on + strand, within GFF1508 at 101.771 kb on + strand, within GFF1508 at 101.771 kb on + strand, within GFF1508 at 101.771 kb on + strand, within GFF1508 at 101.771 kb on + strand, within GFF1508 at 101.820 kb on - strand, within GFF1508 at 101.820 kb on - strand, within GFF1508 at 101.822 kb on + strand, within GFF1508 at 101.822 kb on + strand, within GFF1508 at 101.822 kb on + strand, within GFF1508 at 101.823 kb on - strand, within GFF1508 at 101.879 kb on + strand, within GFF1508 at 101.879 kb on + strand, within GFF1508 at 101.879 kb on + strand, within GFF1508 at 101.879 kb on + strand, within GFF1508 at 101.880 kb on - strand, within GFF1508 at 101.880 kb on - strand, within GFF1508 at 101.880 kb on - strand, within GFF1508 at 102.074 kb on + strand, within GFF1508 at 102.110 kb on + strand, within GFF1508 at 102.111 kb on - strand, within GFF1508 at 102.111 kb on - strand, within GFF1508 at 102.116 kb on + strand, within GFF1508 at 102.116 kb on + strand, within GFF1508 at 102.116 kb on + strand, within GFF1508 at 102.117 kb on - strand, within GFF1508 at 102.117 kb on - strand, within GFF1508 at 102.185 kb on + strand, within GFF1508 at 102.186 kb on - strand, within GFF1508 at 102.321 kb on - strand at 102.321 kb on - strand at 102.379 kb on + strand at 102.380 kb on - strand at 102.380 kb on - strand at 102.380 kb on - strand at 102.436 kb on + strand, within GFF1509 at 102.437 kb on - strand, within GFF1509 at 102.437 kb on - strand, within GFF1509 at 102.437 kb on - strand, within GFF1509 at 102.655 kb on + strand, within GFF1509 at 102.656 kb on - strand, within GFF1509 at 102.656 kb on - strand, within GFF1509 at 102.656 kb on - strand, within GFF1509 at 103.120 kb on + strand, within GFF1509 at 103.351 kb on - strand at 103.404 kb on + strand at 103.404 kb on + strand at 103.405 kb on - strand at 103.542 kb on + strand, within GFF1510 at 103.542 kb on + strand, within GFF1510 at 103.543 kb on - strand, within GFF1510 at 103.543 kb on - strand, within GFF1510 at 103.923 kb on + strand, within GFF1510 at 103.923 kb on + strand, within GFF1510 at 103.923 kb on + strand, within GFF1510 at 103.923 kb on + strand, within GFF1510 at 103.923 kb on + strand, within GFF1510 at 103.923 kb on + strand, within GFF1510 at 103.923 kb on + strand, within GFF1510 at 103.924 kb on - strand, within GFF1510 at 103.924 kb on - strand, within GFF1510 at 103.924 kb on - strand, within GFF1510 at 103.924 kb on - strand, within GFF1510 at 104.268 kb on + strand, within GFF1510 at 104.268 kb on + strand, within GFF1510 at 104.268 kb on + strand, within GFF1510 at 104.268 kb on + strand, within GFF1510 at 104.269 kb on - strand, within GFF1510
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 101,546 + GFF1508 0.12 -0.6 101,547 - GFF1508 0.12 +2.5 101,570 + GFF1508 0.14 +0.2 101,771 + GFF1508 0.37 -1.7 101,771 + GFF1508 0.37 -1.9 101,771 + GFF1508 0.37 +0.4 101,771 + GFF1508 0.37 +1.8 101,820 - GFF1508 0.42 +0.2 101,820 - GFF1508 0.42 +0.4 101,822 + GFF1508 0.42 +0.0 101,822 + GFF1508 0.42 -0.0 101,822 + GFF1508 0.42 +2.1 101,823 - GFF1508 0.43 -0.4 101,879 + GFF1508 0.49 -0.7 101,879 + GFF1508 0.49 -0.2 101,879 + GFF1508 0.49 -0.2 101,879 + GFF1508 0.49 +0.2 101,880 - GFF1508 0.49 -1.5 101,880 - GFF1508 0.49 +0.5 101,880 - GFF1508 0.49 +0.8 102,074 + GFF1508 0.70 +0.5 102,110 + GFF1508 0.74 -0.1 102,111 - GFF1508 0.75 +0.6 102,111 - GFF1508 0.75 +1.0 102,116 + GFF1508 0.75 +0.1 102,116 + GFF1508 0.75 +0.2 102,116 + GFF1508 0.75 -0.0 102,117 - GFF1508 0.75 -1.4 102,117 - GFF1508 0.75 -2.0 102,185 + GFF1508 0.83 -1.4 102,186 - GFF1508 0.83 +0.2 102,321 - -0.1 102,321 - -0.2 102,379 + -1.2 102,380 - +1.2 102,380 - -0.6 102,380 - -1.5 102,436 + GFF1509 0.10 +0.0 102,437 - GFF1509 0.10 +0.3 102,437 - GFF1509 0.10 -1.3 102,437 - GFF1509 0.10 -1.1 102,655 + GFF1509 0.33 +0.4 102,656 - GFF1509 0.33 -1.8 102,656 - GFF1509 0.33 +0.2 102,656 - GFF1509 0.33 -0.5 103,120 + GFF1509 0.80 -2.2 103,351 - +0.2 103,404 + +0.0 103,404 + +0.4 103,405 - -2.3 103,542 + GFF1510 0.16 -1.0 103,542 + GFF1510 0.16 -0.8 103,543 - GFF1510 0.16 +0.2 103,543 - GFF1510 0.16 +1.6 103,923 + GFF1510 0.41 -0.8 103,923 + GFF1510 0.41 +1.1 103,923 + GFF1510 0.41 -1.2 103,923 + GFF1510 0.41 -1.7 103,923 + GFF1510 0.41 -2.3 103,923 + GFF1510 0.41 -0.8 103,923 + GFF1510 0.41 -1.0 103,924 - GFF1510 0.41 -0.4 103,924 - GFF1510 0.41 +0.5 103,924 - GFF1510 0.41 -0.4 103,924 - GFF1510 0.41 +0.2 104,268 + GFF1510 0.64 -1.5 104,268 + GFF1510 0.64 +0.6 104,268 + GFF1510 0.64 +1.0 104,268 + GFF1510 0.64 -0.9 104,269 - GFF1510 0.65 +0.6
Or see this region's nucleotide sequence