Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1379 and GFF1380 are separated by 52 nucleotides GFF1380 and GFF1381 overlap by 19 nucleotides GFF1381 and GFF1382 are separated by 198 nucleotides
GFF1379 - Uncharacterized MFS-type transporter, at 724,899 to 726,197
GFF1379
GFF1380 - Peptidase M48, Ste24p, at 726,250 to 727,176
GFF1380
GFF1381 - Peptidase M48, Ste24p, at 727,158 to 728,135
GFF1381
GFF1382 - Short-chain dehydrogenase, associated with 2-hydroxychromene-2-carboxylate isomerase family protein, at 728,334 to 729,131
GFF1382
Position (kb) 727
728
729 Strain fitness (log2 ratio) -2
-1
0
1 at 726.251 kb on + strand at 726.252 kb on - strand at 726.333 kb on - strand at 726.333 kb on - strand at 726.471 kb on - strand, within GFF1380 at 726.471 kb on - strand, within GFF1380 at 726.636 kb on - strand, within GFF1380 at 726.683 kb on + strand, within GFF1380 at 726.684 kb on - strand, within GFF1380 at 726.839 kb on + strand, within GFF1380 at 726.839 kb on + strand, within GFF1380 at 726.840 kb on - strand, within GFF1380 at 726.992 kb on + strand, within GFF1380 at 727.128 kb on - strand at 727.187 kb on + strand at 727.188 kb on - strand at 727.428 kb on + strand, within GFF1381 at 727.546 kb on + strand, within GFF1381 at 727.546 kb on + strand, within GFF1381 at 727.547 kb on - strand, within GFF1381 at 727.597 kb on + strand, within GFF1381 at 727.597 kb on + strand, within GFF1381 at 727.598 kb on - strand, within GFF1381 at 727.598 kb on - strand, within GFF1381 at 727.600 kb on + strand, within GFF1381 at 727.600 kb on + strand, within GFF1381 at 727.600 kb on + strand, within GFF1381 at 727.600 kb on + strand, within GFF1381 at 727.600 kb on + strand, within GFF1381 at 727.600 kb on + strand, within GFF1381 at 727.601 kb on - strand, within GFF1381 at 727.601 kb on - strand, within GFF1381 at 727.601 kb on - strand, within GFF1381 at 727.601 kb on - strand, within GFF1381 at 727.720 kb on + strand, within GFF1381 at 727.721 kb on - strand, within GFF1381 at 727.927 kb on + strand, within GFF1381 at 727.927 kb on + strand, within GFF1381 at 727.927 kb on + strand, within GFF1381 at 727.928 kb on - strand, within GFF1381 at 727.993 kb on - strand, within GFF1381 at 728.032 kb on + strand, within GFF1381 at 728.033 kb on - strand, within GFF1381 at 728.033 kb on - strand, within GFF1381 at 728.139 kb on + strand at 728.173 kb on + strand at 728.174 kb on - strand at 728.272 kb on - strand at 728.272 kb on - strand at 728.290 kb on + strand at 728.291 kb on - strand at 728.314 kb on + strand at 728.314 kb on + strand at 728.314 kb on + strand at 728.315 kb on - strand at 728.315 kb on - strand at 728.315 kb on - strand at 728.316 kb on + strand at 728.316 kb on + strand at 728.316 kb on + strand at 728.317 kb on - strand at 728.317 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 726,251 + -1.0 726,252 - -0.3 726,333 - +0.0 726,333 - +0.3 726,471 - GFF1380 0.24 +0.1 726,471 - GFF1380 0.24 -0.9 726,636 - GFF1380 0.42 -0.8 726,683 + GFF1380 0.47 +0.2 726,684 - GFF1380 0.47 +0.8 726,839 + GFF1380 0.64 +0.3 726,839 + GFF1380 0.64 -1.5 726,840 - GFF1380 0.64 -0.8 726,992 + GFF1380 0.80 +0.1 727,128 - -0.1 727,187 + -1.7 727,188 - -0.5 727,428 + GFF1381 0.28 +0.0 727,546 + GFF1381 0.40 -2.0 727,546 + GFF1381 0.40 -1.0 727,547 - GFF1381 0.40 -1.3 727,597 + GFF1381 0.45 -0.2 727,597 + GFF1381 0.45 +0.1 727,598 - GFF1381 0.45 +0.6 727,598 - GFF1381 0.45 -1.1 727,600 + GFF1381 0.45 -0.1 727,600 + GFF1381 0.45 -0.1 727,600 + GFF1381 0.45 -0.7 727,600 + GFF1381 0.45 +0.1 727,600 + GFF1381 0.45 +0.3 727,600 + GFF1381 0.45 +0.7 727,601 - GFF1381 0.45 +1.2 727,601 - GFF1381 0.45 -0.5 727,601 - GFF1381 0.45 +0.2 727,601 - GFF1381 0.45 -0.2 727,720 + GFF1381 0.57 -0.5 727,721 - GFF1381 0.58 -0.4 727,927 + GFF1381 0.79 -1.1 727,927 + GFF1381 0.79 +0.5 727,927 + GFF1381 0.79 -0.7 727,928 - GFF1381 0.79 -0.3 727,993 - GFF1381 0.85 -1.1 728,032 + GFF1381 0.89 -0.6 728,033 - GFF1381 0.89 -0.9 728,033 - GFF1381 0.89 -0.3 728,139 + -1.0 728,173 + +1.0 728,174 - +0.5 728,272 - -0.0 728,272 - -1.1 728,290 + -0.2 728,291 - -1.2 728,314 + +0.5 728,314 + +0.3 728,314 + -0.1 728,315 - +0.5 728,315 - -1.1 728,315 - -0.8 728,316 + +0.4 728,316 + -0.5 728,316 + -0.1 728,317 - +0.7 728,317 - -0.8
Or see this region's nucleotide sequence