Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF131 and GFF132 are separated by 120 nucleotides GFF132 and GFF133 are separated by 35 nucleotides GFF133 and GFF134 overlap by 4 nucleotides
GFF131 - L-glyceraldehyde 3-phosphate reductase, at 159,327 to 160,367
GFF131
GFF132 - ABC transporter, substrate-binding protein (cluster 10, nitrate/sulfonate/bicarbonate), at 160,488 to 161,459
GFF132
GFF133 - Ferredoxin, at 161,495 to 161,824
GFF133
GFF134 - putative oxidoreductase (AsfA-like), putative L-aspartate oxidase( EC:1.4.3.16 ), at 161,821 to 163,443
GFF134
Position (kb) 160
161
162 Strain fitness (log2 ratio) -2
-1
0
1
2 at 159.667 kb on + strand, within GFF131 at 159.667 kb on + strand, within GFF131 at 159.667 kb on + strand, within GFF131 at 159.667 kb on + strand, within GFF131 at 159.668 kb on - strand, within GFF131 at 159.668 kb on - strand, within GFF131 at 159.778 kb on + strand, within GFF131 at 159.779 kb on - strand, within GFF131 at 159.856 kb on + strand, within GFF131 at 159.857 kb on - strand, within GFF131 at 159.857 kb on - strand, within GFF131 at 159.874 kb on + strand, within GFF131 at 159.874 kb on + strand, within GFF131 at 159.874 kb on + strand, within GFF131 at 159.874 kb on + strand, within GFF131 at 159.875 kb on - strand, within GFF131 at 159.875 kb on - strand, within GFF131 at 159.958 kb on + strand, within GFF131 at 159.973 kb on + strand, within GFF131 at 160.021 kb on + strand, within GFF131 at 160.022 kb on - strand, within GFF131 at 160.022 kb on - strand, within GFF131 at 160.048 kb on + strand, within GFF131 at 160.172 kb on - strand, within GFF131 at 160.172 kb on - strand, within GFF131 at 160.360 kb on + strand at 160.360 kb on + strand at 160.361 kb on - strand at 160.430 kb on + strand at 160.681 kb on + strand, within GFF132 at 160.682 kb on - strand, within GFF132 at 160.684 kb on + strand, within GFF132 at 160.684 kb on + strand, within GFF132 at 160.996 kb on + strand, within GFF132 at 160.996 kb on + strand, within GFF132 at 160.997 kb on - strand, within GFF132 at 161.008 kb on + strand, within GFF132 at 161.008 kb on + strand, within GFF132 at 161.008 kb on + strand, within GFF132 at 161.009 kb on - strand, within GFF132 at 161.009 kb on - strand, within GFF132 at 161.170 kb on + strand, within GFF132 at 161.170 kb on + strand, within GFF132 at 161.580 kb on + strand, within GFF133 at 161.581 kb on - strand, within GFF133 at 161.655 kb on + strand, within GFF133 at 161.676 kb on + strand, within GFF133 at 161.676 kb on + strand, within GFF133 at 161.972 kb on + strand at 161.973 kb on - strand at 162.389 kb on + strand, within GFF134
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 159,667 + GFF131 0.33 -0.2 159,667 + GFF131 0.33 -0.2 159,667 + GFF131 0.33 -0.5 159,667 + GFF131 0.33 +0.4 159,668 - GFF131 0.33 +0.4 159,668 - GFF131 0.33 -0.8 159,778 + GFF131 0.43 +0.1 159,779 - GFF131 0.43 -1.6 159,856 + GFF131 0.51 -0.9 159,857 - GFF131 0.51 -0.0 159,857 - GFF131 0.51 -1.6 159,874 + GFF131 0.53 +1.4 159,874 + GFF131 0.53 -0.7 159,874 + GFF131 0.53 +0.2 159,874 + GFF131 0.53 -1.1 159,875 - GFF131 0.53 +0.2 159,875 - GFF131 0.53 -0.1 159,958 + GFF131 0.61 -0.8 159,973 + GFF131 0.62 -1.2 160,021 + GFF131 0.67 +0.0 160,022 - GFF131 0.67 +0.4 160,022 - GFF131 0.67 -0.2 160,048 + GFF131 0.69 -0.2 160,172 - GFF131 0.81 +0.4 160,172 - GFF131 0.81 +0.2 160,360 + -1.3 160,360 + -0.1 160,361 - +0.6 160,430 + -0.5 160,681 + GFF132 0.20 -1.9 160,682 - GFF132 0.20 -0.8 160,684 + GFF132 0.20 -1.1 160,684 + GFF132 0.20 -0.8 160,996 + GFF132 0.52 -0.2 160,996 + GFF132 0.52 +0.4 160,997 - GFF132 0.52 -0.2 161,008 + GFF132 0.53 -0.1 161,008 + GFF132 0.53 -1.4 161,008 + GFF132 0.53 +0.2 161,009 - GFF132 0.54 -0.1 161,009 - GFF132 0.54 -0.0 161,170 + GFF132 0.70 -0.6 161,170 + GFF132 0.70 +0.4 161,580 + GFF133 0.26 +0.1 161,581 - GFF133 0.26 +0.4 161,655 + GFF133 0.48 +0.3 161,676 + GFF133 0.55 +0.3 161,676 + GFF133 0.55 +0.9 161,972 + +0.3 161,973 - +0.1 162,389 + GFF134 0.35 +1.8
Or see this region's nucleotide sequence