Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1290 and GFF1291 overlap by 4 nucleotides GFF1291 and GFF1292 are separated by 211 nucleotides GFF1292 and GFF1293 overlap by 4 nucleotides
GFF1290 - Sulfate and thiosulfate import ATP-binding protein CysA (EC 3.6.3.25), at 617,121 to 618,218
GFF1290
GFF1291 - Periplasmic thiol:disulfide oxidoreductase DsbB, required for DsbA reoxidation, at 618,215 to 618,718
GFF1291
GFF1292 - Macrolide-specific efflux protein MacA, at 618,930 to 620,087
GFF1292
GFF1293 - Macrolide export ATP-binding/permease protein MacB, at 620,084 to 622,093
GFF1293
Position (kb) 618
619
620
621 Strain fitness (log2 ratio) -1
0
1
2
3 at 617.943 kb on + strand, within GFF1290 at 617.943 kb on + strand, within GFF1290 at 617.976 kb on + strand, within GFF1290 at 617.976 kb on + strand, within GFF1290 at 617.976 kb on + strand, within GFF1290 at 617.977 kb on - strand, within GFF1290 at 617.977 kb on - strand, within GFF1290 at 617.977 kb on - strand, within GFF1290 at 618.193 kb on + strand at 618.193 kb on + strand at 618.193 kb on + strand at 618.193 kb on + strand at 618.194 kb on - strand at 618.194 kb on - strand at 618.208 kb on + strand at 618.208 kb on + strand at 618.208 kb on + strand at 618.829 kb on + strand at 618.830 kb on - strand at 618.840 kb on - strand at 618.887 kb on - strand at 618.938 kb on + strand at 618.963 kb on + strand at 618.963 kb on + strand at 618.964 kb on - strand at 618.964 kb on - strand at 618.964 kb on - strand at 618.964 kb on - strand at 619.041 kb on + strand at 619.041 kb on + strand at 619.041 kb on + strand at 619.041 kb on + strand at 619.042 kb on - strand at 619.042 kb on - strand at 619.483 kb on - strand, within GFF1292 at 619.483 kb on - strand, within GFF1292 at 619.665 kb on + strand, within GFF1292 at 619.665 kb on + strand, within GFF1292 at 619.665 kb on + strand, within GFF1292 at 619.665 kb on + strand, within GFF1292 at 619.666 kb on - strand, within GFF1292 at 619.699 kb on - strand, within GFF1292 at 619.701 kb on + strand, within GFF1292 at 619.701 kb on + strand, within GFF1292 at 619.701 kb on + strand, within GFF1292 at 619.701 kb on + strand, within GFF1292 at 619.701 kb on + strand, within GFF1292 at 619.702 kb on - strand, within GFF1292 at 620.300 kb on + strand, within GFF1293 at 620.300 kb on + strand, within GFF1293 at 620.300 kb on + strand, within GFF1293 at 620.300 kb on + strand, within GFF1293 at 620.301 kb on - strand, within GFF1293 at 620.301 kb on - strand, within GFF1293 at 620.301 kb on - strand, within GFF1293 at 620.301 kb on - strand, within GFF1293 at 620.301 kb on - strand, within GFF1293 at 620.390 kb on + strand, within GFF1293 at 620.390 kb on + strand, within GFF1293 at 620.434 kb on - strand, within GFF1293 at 620.444 kb on + strand, within GFF1293 at 620.444 kb on + strand, within GFF1293 at 620.445 kb on - strand, within GFF1293 at 621.060 kb on - strand, within GFF1293
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 617,943 + GFF1290 0.75 -0.5 617,943 + GFF1290 0.75 +1.5 617,976 + GFF1290 0.78 -0.2 617,976 + GFF1290 0.78 +0.2 617,976 + GFF1290 0.78 -1.0 617,977 - GFF1290 0.78 -0.6 617,977 - GFF1290 0.78 +0.5 617,977 - GFF1290 0.78 -0.3 618,193 + +1.5 618,193 + -0.3 618,193 + +0.1 618,193 + -0.4 618,194 - +2.8 618,194 - +0.0 618,208 + -0.3 618,208 + -0.1 618,208 + -0.4 618,829 + -0.2 618,830 - -0.9 618,840 - -0.4 618,887 - -0.3 618,938 + -0.0 618,963 + -0.0 618,963 + -0.1 618,964 - +0.7 618,964 - +2.1 618,964 - -0.8 618,964 - +0.4 619,041 + +0.2 619,041 + +0.1 619,041 + +0.8 619,041 + -0.0 619,042 - -0.3 619,042 - +0.2 619,483 - GFF1292 0.48 -0.6 619,483 - GFF1292 0.48 -1.2 619,665 + GFF1292 0.63 +0.1 619,665 + GFF1292 0.63 +0.9 619,665 + GFF1292 0.63 -1.2 619,665 + GFF1292 0.63 -0.1 619,666 - GFF1292 0.64 -0.2 619,699 - GFF1292 0.66 -0.6 619,701 + GFF1292 0.67 -1.0 619,701 + GFF1292 0.67 +0.0 619,701 + GFF1292 0.67 -0.9 619,701 + GFF1292 0.67 -0.1 619,701 + GFF1292 0.67 -0.9 619,702 - GFF1292 0.67 -1.2 620,300 + GFF1293 0.11 +0.2 620,300 + GFF1293 0.11 +1.5 620,300 + GFF1293 0.11 +0.5 620,300 + GFF1293 0.11 -0.4 620,301 - GFF1293 0.11 -0.1 620,301 - GFF1293 0.11 -1.0 620,301 - GFF1293 0.11 -0.9 620,301 - GFF1293 0.11 +2.0 620,301 - GFF1293 0.11 +0.3 620,390 + GFF1293 0.15 +0.3 620,390 + GFF1293 0.15 +0.2 620,434 - GFF1293 0.17 +0.2 620,444 + GFF1293 0.18 -0.4 620,444 + GFF1293 0.18 -0.3 620,445 - GFF1293 0.18 -0.1 621,060 - GFF1293 0.49 +2.3
Or see this region's nucleotide sequence