Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1277 and GFF1278 are separated by 52 nucleotides GFF1278 and GFF1279 overlap by 4 nucleotides GFF1279 and GFF1280 are separated by 13 nucleotides GFF1280 and GFF1281 overlap by 10 nucleotides
GFF1277 - Alkanesulfonates-binding protein, at 607,159 to 608,130
GFF1277
GFF1278 - no description, at 608,183 to 608,305
GFF1278
GFF1279 - ABC transporter, substrate-binding protein (cluster 3, basic aa/glutamine/opines), at 608,302 to 609,135
GFF1279
GFF1280 - ABC transporter, permease protein (cluster 3, basic aa/glutamine/opines), at 609,149 to 609,856
GFF1280
GFF1281 - ABC transporter, permease protein (cluster 3, basic aa/glutamine/opines), at 609,847 to 610,578
GFF1281
Position (kb)
608
609
610 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 607.469 kb on + strand, within GFF1277 at 607.469 kb on + strand, within GFF1277 at 607.470 kb on - strand, within GFF1277 at 607.470 kb on - strand, within GFF1277 at 607.587 kb on - strand, within GFF1277 at 607.587 kb on - strand, within GFF1277 at 607.619 kb on + strand, within GFF1277 at 607.619 kb on + strand, within GFF1277 at 607.619 kb on + strand, within GFF1277 at 607.620 kb on - strand, within GFF1277 at 607.620 kb on - strand, within GFF1277 at 607.620 kb on - strand, within GFF1277 at 607.679 kb on + strand, within GFF1277 at 607.680 kb on - strand, within GFF1277 at 607.680 kb on - strand, within GFF1277 at 607.680 kb on - strand, within GFF1277 at 607.680 kb on - strand, within GFF1277 at 607.680 kb on - strand, within GFF1277 at 607.680 kb on - strand, within GFF1277 at 607.680 kb on - strand, within GFF1277 at 607.692 kb on - strand, within GFF1277 at 607.692 kb on - strand, within GFF1277 at 607.805 kb on + strand, within GFF1277 at 607.806 kb on - strand, within GFF1277 at 607.806 kb on - strand, within GFF1277 at 608.061 kb on + strand at 608.155 kb on + strand at 608.155 kb on + strand at 608.155 kb on + strand at 608.155 kb on + strand at 608.156 kb on - strand at 608.156 kb on - strand at 608.156 kb on - strand at 608.221 kb on + strand, within GFF1278 at 608.221 kb on + strand, within GFF1278 at 608.221 kb on + strand, within GFF1278 at 608.221 kb on + strand, within GFF1278 at 608.221 kb on + strand, within GFF1278 at 608.221 kb on + strand, within GFF1278 at 608.222 kb on - strand, within GFF1278 at 608.222 kb on - strand, within GFF1278 at 608.222 kb on - strand, within GFF1278 at 608.222 kb on - strand, within GFF1278 at 608.222 kb on - strand, within GFF1278 at 608.266 kb on + strand, within GFF1278 at 608.266 kb on + strand, within GFF1278 at 608.266 kb on + strand, within GFF1278 at 608.284 kb on + strand, within GFF1278 at 608.284 kb on + strand, within GFF1278 at 608.284 kb on + strand, within GFF1278 at 608.285 kb on - strand, within GFF1278 at 608.453 kb on - strand, within GFF1279 at 608.659 kb on + strand, within GFF1279 at 608.660 kb on - strand, within GFF1279 at 608.660 kb on - strand, within GFF1279 at 608.783 kb on - strand, within GFF1279 at 608.783 kb on - strand, within GFF1279 at 608.806 kb on + strand, within GFF1279 at 608.807 kb on - strand, within GFF1279 at 608.807 kb on - strand, within GFF1279 at 608.807 kb on - strand, within GFF1279 at 608.942 kb on + strand, within GFF1279 at 608.953 kb on + strand, within GFF1279 at 608.953 kb on + strand, within GFF1279 at 608.953 kb on + strand, within GFF1279 at 608.954 kb on - strand, within GFF1279 at 609.052 kb on + strand at 609.052 kb on + strand at 609.052 kb on + strand at 609.053 kb on - strand at 609.053 kb on - strand at 609.053 kb on - strand at 609.053 kb on - strand at 609.329 kb on + strand, within GFF1280 at 609.329 kb on + strand, within GFF1280 at 609.329 kb on + strand, within GFF1280 at 609.329 kb on + strand, within GFF1280 at 609.329 kb on + strand, within GFF1280 at 609.329 kb on + strand, within GFF1280 at 609.330 kb on - strand, within GFF1280 at 609.330 kb on - strand, within GFF1280 at 609.330 kb on - strand, within GFF1280 at 609.330 kb on - strand, within GFF1280 at 609.330 kb on - strand, within GFF1280 at 609.330 kb on - strand, within GFF1280 at 609.330 kb on - strand, within GFF1280 at 609.380 kb on + strand, within GFF1280 at 609.380 kb on + strand, within GFF1280 at 609.380 kb on + strand, within GFF1280 at 609.428 kb on + strand, within GFF1280 at 609.428 kb on + strand, within GFF1280 at 609.428 kb on + strand, within GFF1280 at 609.428 kb on + strand, within GFF1280 at 609.429 kb on - strand, within GFF1280 at 609.429 kb on - strand, within GFF1280 at 609.429 kb on - strand, within GFF1280 at 609.429 kb on - strand, within GFF1280 at 609.429 kb on - strand, within GFF1280 at 609.485 kb on + strand, within GFF1280 at 609.497 kb on + strand, within GFF1280 at 609.497 kb on + strand, within GFF1280 at 609.497 kb on + strand, within GFF1280 at 609.497 kb on + strand, within GFF1280 at 609.497 kb on + strand, within GFF1280 at 609.497 kb on + strand, within GFF1280 at 609.497 kb on + strand, within GFF1280 at 609.497 kb on + strand, within GFF1280 at 609.498 kb on - strand, within GFF1280 at 609.498 kb on - strand, within GFF1280 at 609.498 kb on - strand, within GFF1280 at 609.498 kb on - strand, within GFF1280 at 609.498 kb on - strand, within GFF1280 at 609.650 kb on + strand, within GFF1280 at 609.650 kb on + strand, within GFF1280 at 609.650 kb on + strand, within GFF1280 at 609.650 kb on + strand, within GFF1280 at 609.650 kb on + strand, within GFF1280 at 609.650 kb on + strand, within GFF1280 at 609.650 kb on + strand, within GFF1280 at 609.650 kb on + strand, within GFF1280 at 609.651 kb on - strand, within GFF1280 at 609.651 kb on - strand, within GFF1280 at 609.651 kb on - strand, within GFF1280 at 609.651 kb on - strand, within GFF1280 at 609.651 kb on - strand, within GFF1280 at 609.651 kb on - strand, within GFF1280 at 609.651 kb on - strand, within GFF1280 at 609.710 kb on + strand, within GFF1280 at 609.711 kb on - strand, within GFF1280 at 609.779 kb on + strand, within GFF1280 at 609.779 kb on + strand, within GFF1280 at 609.779 kb on + strand, within GFF1280 at 609.780 kb on - strand, within GFF1280 at 609.780 kb on - strand, within GFF1280 at 609.780 kb on - strand, within GFF1280 at 609.780 kb on - strand, within GFF1280 at 609.884 kb on - strand at 609.884 kb on - strand at 610.093 kb on + strand, within GFF1281 at 610.093 kb on + strand, within GFF1281 at 610.093 kb on + strand, within GFF1281 at 610.093 kb on + strand, within GFF1281 at 610.093 kb on + strand, within GFF1281 at 610.093 kb on + strand, within GFF1281 at 610.094 kb on - strand, within GFF1281 at 610.094 kb on - strand, within GFF1281 at 610.094 kb on - strand, within GFF1281
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 607,469 + GFF1277 0.32 +0.5 607,469 + GFF1277 0.32 +0.6 607,470 - GFF1277 0.32 -0.5 607,470 - GFF1277 0.32 +0.1 607,587 - GFF1277 0.44 +0.3 607,587 - GFF1277 0.44 +0.2 607,619 + GFF1277 0.47 +0.5 607,619 + GFF1277 0.47 -1.3 607,619 + GFF1277 0.47 -1.5 607,620 - GFF1277 0.47 -1.0 607,620 - GFF1277 0.47 -0.0 607,620 - GFF1277 0.47 +0.6 607,679 + GFF1277 0.53 +0.1 607,680 - GFF1277 0.54 +1.2 607,680 - GFF1277 0.54 -0.2 607,680 - GFF1277 0.54 -1.0 607,680 - GFF1277 0.54 -0.2 607,680 - GFF1277 0.54 +0.5 607,680 - GFF1277 0.54 -0.2 607,680 - GFF1277 0.54 +0.5 607,692 - GFF1277 0.55 -0.3 607,692 - GFF1277 0.55 -0.3 607,805 + GFF1277 0.66 +0.4 607,806 - GFF1277 0.67 -1.5 607,806 - GFF1277 0.67 +0.1 608,061 + -0.0 608,155 + +0.0 608,155 + +0.6 608,155 + -0.2 608,155 + -0.3 608,156 - +0.5 608,156 - +0.2 608,156 - -1.8 608,221 + GFF1278 0.31 +1.8 608,221 + GFF1278 0.31 -0.4 608,221 + GFF1278 0.31 +0.2 608,221 + GFF1278 0.31 -0.5 608,221 + GFF1278 0.31 -0.5 608,221 + GFF1278 0.31 -1.0 608,222 - GFF1278 0.32 -1.5 608,222 - GFF1278 0.32 -0.8 608,222 - GFF1278 0.32 -1.0 608,222 - GFF1278 0.32 +0.1 608,222 - GFF1278 0.32 +0.3 608,266 + GFF1278 0.67 +0.3 608,266 + GFF1278 0.67 -0.2 608,266 + GFF1278 0.67 +0.1 608,284 + GFF1278 0.82 -1.1 608,284 + GFF1278 0.82 +1.9 608,284 + GFF1278 0.82 -0.4 608,285 - GFF1278 0.83 -0.2 608,453 - GFF1279 0.18 +0.7 608,659 + GFF1279 0.43 +0.7 608,660 - GFF1279 0.43 -0.2 608,660 - GFF1279 0.43 -0.4 608,783 - GFF1279 0.58 +0.0 608,783 - GFF1279 0.58 -2.5 608,806 + GFF1279 0.60 +1.1 608,807 - GFF1279 0.61 +1.2 608,807 - GFF1279 0.61 -1.8 608,807 - GFF1279 0.61 -0.0 608,942 + GFF1279 0.77 +0.3 608,953 + GFF1279 0.78 +1.5 608,953 + GFF1279 0.78 -1.2 608,953 + GFF1279 0.78 +0.5 608,954 - GFF1279 0.78 -0.0 609,052 + +0.2 609,052 + -1.4 609,052 + +0.5 609,053 - -0.1 609,053 - +0.2 609,053 - -0.6 609,053 - -0.8 609,329 + GFF1280 0.25 -0.1 609,329 + GFF1280 0.25 -1.0 609,329 + GFF1280 0.25 -1.1 609,329 + GFF1280 0.25 +0.2 609,329 + GFF1280 0.25 +0.2 609,329 + GFF1280 0.25 -1.5 609,330 - GFF1280 0.26 -0.7 609,330 - GFF1280 0.26 +0.6 609,330 - GFF1280 0.26 +0.0 609,330 - GFF1280 0.26 -0.2 609,330 - GFF1280 0.26 +1.8 609,330 - GFF1280 0.26 +0.2 609,330 - GFF1280 0.26 -1.0 609,380 + GFF1280 0.33 +0.9 609,380 + GFF1280 0.33 -0.3 609,380 + GFF1280 0.33 +0.8 609,428 + GFF1280 0.39 -0.7 609,428 + GFF1280 0.39 +0.5 609,428 + GFF1280 0.39 +0.0 609,428 + GFF1280 0.39 +1.3 609,429 - GFF1280 0.40 -1.3 609,429 - GFF1280 0.40 -0.4 609,429 - GFF1280 0.40 -1.0 609,429 - GFF1280 0.40 -0.6 609,429 - GFF1280 0.40 -0.9 609,485 + GFF1280 0.47 -0.6 609,497 + GFF1280 0.49 -0.5 609,497 + GFF1280 0.49 -1.2 609,497 + GFF1280 0.49 +0.2 609,497 + GFF1280 0.49 -2.2 609,497 + GFF1280 0.49 -0.2 609,497 + GFF1280 0.49 -0.3 609,497 + GFF1280 0.49 -1.0 609,497 + GFF1280 0.49 -0.2 609,498 - GFF1280 0.49 -1.0 609,498 - GFF1280 0.49 -0.1 609,498 - GFF1280 0.49 -2.7 609,498 - GFF1280 0.49 -0.4 609,498 - GFF1280 0.49 -0.5 609,650 + GFF1280 0.71 -0.7 609,650 + GFF1280 0.71 +0.5 609,650 + GFF1280 0.71 -1.1 609,650 + GFF1280 0.71 -0.6 609,650 + GFF1280 0.71 +0.1 609,650 + GFF1280 0.71 -0.4 609,650 + GFF1280 0.71 +1.1 609,650 + GFF1280 0.71 -0.4 609,651 - GFF1280 0.71 +0.8 609,651 - GFF1280 0.71 -0.5 609,651 - GFF1280 0.71 -0.3 609,651 - GFF1280 0.71 -0.5 609,651 - GFF1280 0.71 -0.1 609,651 - GFF1280 0.71 -1.0 609,651 - GFF1280 0.71 +0.3 609,710 + GFF1280 0.79 +0.2 609,711 - GFF1280 0.79 +0.5 609,779 + GFF1280 0.89 +0.0 609,779 + GFF1280 0.89 -0.9 609,779 + GFF1280 0.89 +0.9 609,780 - GFF1280 0.89 +0.2 609,780 - GFF1280 0.89 +0.8 609,780 - GFF1280 0.89 -0.2 609,780 - GFF1280 0.89 -0.7 609,884 - -1.3 609,884 - -1.8 610,093 + GFF1281 0.34 -0.1 610,093 + GFF1281 0.34 +0.4 610,093 + GFF1281 0.34 -0.7 610,093 + GFF1281 0.34 +0.5 610,093 + GFF1281 0.34 -1.3 610,093 + GFF1281 0.34 +0.3 610,094 - GFF1281 0.34 -0.7 610,094 - GFF1281 0.34 -0.5 610,094 - GFF1281 0.34 +3.5
Or see this region's nucleotide sequence