Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1040 and GFF1041 are separated by 18 nucleotides GFF1041 and GFF1043 overlap by 4 nucleotides GFF1043 and GFF1042 overlap by 2 nucleotides GFF1042 and GFF1044 are separated by 13 nucleotides
GFF1040 - Oxidoreductase, short-chain dehydrogenase/reductase family, at 357,342 to 358,244
GFF1040
GFF1041 - Transcriptional regulator, AsnC family, at 358,263 to 358,721
GFF1041
GFF1043 - no description, at 358,718 to 358,846
GFF1043
GFF1042 - Glutathione S-transferase, unnamed subgroup 2 (EC 2.5.1.18), at 358,845 to 359,453
GFF1042
GFF1044 - hypothetical protein, at 359,467 to 359,856
GFF1044
Position (kb) 358
359 Strain fitness (log2 ratio) -2
-1
0
1
2 at 357.270 kb on + strand at 357.270 kb on + strand at 357.270 kb on + strand at 357.270 kb on + strand at 357.271 kb on - strand at 357.271 kb on - strand at 357.271 kb on - strand at 357.271 kb on - strand at 357.271 kb on - strand at 357.295 kb on - strand at 357.711 kb on + strand, within GFF1040 at 357.711 kb on + strand, within GFF1040 at 357.711 kb on + strand, within GFF1040 at 357.711 kb on + strand, within GFF1040 at 357.711 kb on + strand, within GFF1040 at 357.711 kb on + strand, within GFF1040 at 357.712 kb on - strand, within GFF1040 at 357.834 kb on + strand, within GFF1040 at 357.834 kb on + strand, within GFF1040 at 357.834 kb on + strand, within GFF1040 at 357.834 kb on + strand, within GFF1040 at 357.834 kb on + strand, within GFF1040 at 357.834 kb on + strand, within GFF1040 at 357.835 kb on - strand, within GFF1040 at 357.835 kb on - strand, within GFF1040 at 357.835 kb on - strand, within GFF1040 at 357.835 kb on - strand, within GFF1040 at 357.835 kb on - strand, within GFF1040 at 357.835 kb on - strand, within GFF1040 at 358.155 kb on + strand at 358.155 kb on + strand at 358.155 kb on + strand at 358.155 kb on + strand at 358.156 kb on - strand at 358.156 kb on - strand at 358.251 kb on - strand at 358.264 kb on + strand at 358.264 kb on + strand at 358.264 kb on + strand at 358.264 kb on + strand at 358.265 kb on - strand at 358.265 kb on - strand at 358.265 kb on - strand at 358.265 kb on - strand at 358.480 kb on + strand, within GFF1041 at 358.480 kb on + strand, within GFF1041 at 358.481 kb on - strand, within GFF1041 at 358.567 kb on + strand, within GFF1041 at 358.567 kb on + strand, within GFF1041 at 358.813 kb on + strand, within GFF1043 at 359.166 kb on + strand, within GFF1042 at 359.166 kb on + strand, within GFF1042 at 359.166 kb on + strand, within GFF1042 at 359.167 kb on - strand, within GFF1042 at 359.244 kb on + strand, within GFF1042 at 359.245 kb on - strand, within GFF1042 at 359.400 kb on + strand at 359.400 kb on + strand at 359.546 kb on + strand, within GFF1044 at 359.546 kb on + strand, within GFF1044 at 359.546 kb on + strand, within GFF1044 at 359.546 kb on + strand, within GFF1044 at 359.546 kb on + strand, within GFF1044 at 359.546 kb on + strand, within GFF1044 at 359.547 kb on - strand, within GFF1044 at 359.547 kb on - strand, within GFF1044 at 359.547 kb on - strand, within GFF1044 at 359.547 kb on - strand, within GFF1044 at 359.547 kb on - strand, within GFF1044 at 359.547 kb on - strand, within GFF1044 at 359.547 kb on - strand, within GFF1044
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 357,270 + +0.0 357,270 + +2.3 357,270 + -1.7 357,270 + +1.6 357,271 - +0.3 357,271 - -1.7 357,271 - -0.2 357,271 - -0.3 357,271 - +1.3 357,295 - -0.1 357,711 + GFF1040 0.41 +0.2 357,711 + GFF1040 0.41 -0.9 357,711 + GFF1040 0.41 +0.3 357,711 + GFF1040 0.41 -0.6 357,711 + GFF1040 0.41 -0.4 357,711 + GFF1040 0.41 -0.4 357,712 - GFF1040 0.41 +0.1 357,834 + GFF1040 0.54 +1.4 357,834 + GFF1040 0.54 -1.7 357,834 + GFF1040 0.54 -0.6 357,834 + GFF1040 0.54 +0.6 357,834 + GFF1040 0.54 -0.1 357,834 + GFF1040 0.54 -0.3 357,835 - GFF1040 0.55 -1.4 357,835 - GFF1040 0.55 -0.5 357,835 - GFF1040 0.55 +1.0 357,835 - GFF1040 0.55 +1.6 357,835 - GFF1040 0.55 -1.4 357,835 - GFF1040 0.55 -1.7 358,155 + -2.0 358,155 + -1.0 358,155 + +0.4 358,155 + +0.0 358,156 - -0.6 358,156 - -0.2 358,251 - -0.4 358,264 + +0.7 358,264 + -0.1 358,264 + +0.0 358,264 + -0.7 358,265 - +0.0 358,265 - +0.3 358,265 - -0.9 358,265 - +0.4 358,480 + GFF1041 0.47 +1.1 358,480 + GFF1041 0.47 +0.1 358,481 - GFF1041 0.47 -0.9 358,567 + GFF1041 0.66 +0.6 358,567 + GFF1041 0.66 -0.1 358,813 + GFF1043 0.74 -0.7 359,166 + GFF1042 0.53 -1.2 359,166 + GFF1042 0.53 +0.3 359,166 + GFF1042 0.53 -0.4 359,167 - GFF1042 0.53 -0.2 359,244 + GFF1042 0.66 -0.1 359,245 - GFF1042 0.66 -0.0 359,400 + +0.0 359,400 + +0.9 359,546 + GFF1044 0.20 -0.5 359,546 + GFF1044 0.20 -0.4 359,546 + GFF1044 0.20 +0.2 359,546 + GFF1044 0.20 +0.0 359,546 + GFF1044 0.20 -0.1 359,546 + GFF1044 0.20 +0.5 359,547 - GFF1044 0.21 -1.1 359,547 - GFF1044 0.21 +0.1 359,547 - GFF1044 0.21 +0.1 359,547 - GFF1044 0.21 -0.1 359,547 - GFF1044 0.21 +1.0 359,547 - GFF1044 0.21 -0.7 359,547 - GFF1044 0.21 -0.4
Or see this region's nucleotide sequence