Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF7377 and GFF7378 overlap by 4 nucleotides GFF7378 and GFF7379 overlap by 4 nucleotides GFF7379 and GFF7380 are separated by 29 nucleotides
GFF7377 - Methylmalonate-semialdehyde dehydrogenase (EC 1.2.1.27), at 7,037 to 8,560
GFF7377
GFF7378 - protein of unknown function DUF1311, at 8,557 to 8,955
GFF7378
GFF7379 - Branched-chain acyl-CoA dehydrogenase (EC 1.3.99.12), at 8,952 to 10,106
GFF7379
GFF7380 - 3-hydroxyisobutyryl-CoA hydrolase (EC 3.1.2.4), at 10,136 to 11,251
GFF7380
Position (kb)
8
9
10
11 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 8.198 kb on + strand, within GFF7377 at 8.198 kb on + strand, within GFF7377 at 8.199 kb on - strand, within GFF7377 at 8.199 kb on - strand, within GFF7377 at 8.199 kb on - strand, within GFF7377 at 8.199 kb on - strand, within GFF7377 at 8.199 kb on - strand, within GFF7377 at 8.199 kb on - strand, within GFF7377 at 8.474 kb on + strand at 8.475 kb on - strand at 8.475 kb on - strand at 8.475 kb on - strand at 8.554 kb on + strand at 8.554 kb on + strand at 8.554 kb on + strand at 8.554 kb on + strand at 8.554 kb on + strand at 8.554 kb on + strand at 8.555 kb on - strand at 8.555 kb on - strand at 8.555 kb on - strand at 8.638 kb on + strand, within GFF7378 at 8.740 kb on + strand, within GFF7378 at 9.111 kb on + strand, within GFF7379 at 9.111 kb on + strand, within GFF7379 at 9.111 kb on + strand, within GFF7379 at 9.111 kb on + strand, within GFF7379 at 9.111 kb on + strand, within GFF7379 at 9.111 kb on + strand, within GFF7379 at 9.111 kb on + strand, within GFF7379 at 9.111 kb on + strand, within GFF7379 at 9.111 kb on + strand, within GFF7379 at 9.112 kb on - strand, within GFF7379 at 9.112 kb on - strand, within GFF7379 at 9.112 kb on - strand, within GFF7379 at 9.112 kb on - strand, within GFF7379 at 9.112 kb on - strand, within GFF7379 at 9.112 kb on - strand, within GFF7379 at 9.112 kb on - strand, within GFF7379 at 9.112 kb on - strand, within GFF7379 at 9.112 kb on - strand, within GFF7379 at 9.112 kb on - strand, within GFF7379 at 9.112 kb on - strand, within GFF7379 at 9.316 kb on - strand, within GFF7379 at 9.316 kb on - strand, within GFF7379 at 9.316 kb on - strand, within GFF7379 at 9.316 kb on - strand, within GFF7379 at 9.316 kb on - strand, within GFF7379 at 9.316 kb on - strand, within GFF7379 at 9.316 kb on - strand, within GFF7379 at 9.316 kb on - strand, within GFF7379 at 9.390 kb on + strand, within GFF7379 at 9.390 kb on + strand, within GFF7379 at 9.390 kb on + strand, within GFF7379 at 9.390 kb on + strand, within GFF7379 at 9.390 kb on + strand, within GFF7379 at 9.390 kb on + strand, within GFF7379 at 9.390 kb on + strand, within GFF7379 at 9.391 kb on - strand, within GFF7379 at 9.391 kb on - strand, within GFF7379 at 9.391 kb on - strand, within GFF7379 at 9.391 kb on - strand, within GFF7379 at 9.391 kb on - strand, within GFF7379 at 9.391 kb on - strand, within GFF7379 at 9.391 kb on - strand, within GFF7379 at 9.391 kb on - strand, within GFF7379 at 9.528 kb on + strand, within GFF7379 at 9.529 kb on - strand, within GFF7379 at 9.529 kb on - strand, within GFF7379 at 9.529 kb on - strand, within GFF7379 at 9.735 kb on + strand, within GFF7379 at 9.736 kb on - strand, within GFF7379 at 9.736 kb on - strand, within GFF7379 at 9.736 kb on - strand, within GFF7379 at 9.891 kb on + strand, within GFF7379 at 9.891 kb on + strand, within GFF7379 at 9.891 kb on + strand, within GFF7379 at 9.891 kb on + strand, within GFF7379 at 9.891 kb on + strand, within GFF7379 at 9.891 kb on + strand, within GFF7379 at 9.891 kb on + strand, within GFF7379 at 9.891 kb on + strand, within GFF7379 at 9.891 kb on + strand, within GFF7379 at 9.891 kb on + strand, within GFF7379 at 9.892 kb on - strand, within GFF7379 at 9.892 kb on - strand, within GFF7379 at 9.892 kb on - strand, within GFF7379 at 9.892 kb on - strand, within GFF7379 at 9.892 kb on - strand, within GFF7379 at 9.892 kb on - strand, within GFF7379 at 9.892 kb on - strand, within GFF7379 at 9.892 kb on - strand, within GFF7379 at 9.892 kb on - strand, within GFF7379 at 9.892 kb on - strand, within GFF7379 at 9.892 kb on - strand, within GFF7379 at 9.892 kb on - strand, within GFF7379 at 9.892 kb on - strand, within GFF7379 at 9.975 kb on + strand, within GFF7379 at 9.976 kb on - strand, within GFF7379 at 9.976 kb on - strand, within GFF7379 at 9.976 kb on - strand, within GFF7379 at 10.149 kb on + strand at 10.149 kb on + strand at 10.149 kb on + strand at 10.149 kb on + strand at 10.149 kb on + strand at 10.149 kb on + strand at 10.149 kb on + strand at 10.149 kb on + strand at 10.149 kb on + strand at 10.149 kb on + strand at 10.149 kb on + strand at 10.149 kb on + strand at 10.149 kb on + strand at 10.149 kb on + strand at 10.149 kb on + strand at 10.150 kb on - strand at 10.150 kb on - strand at 10.150 kb on - strand at 10.150 kb on - strand at 10.150 kb on - strand at 10.150 kb on - strand at 10.150 kb on - strand at 10.150 kb on - strand at 10.150 kb on - strand at 10.150 kb on - strand at 10.150 kb on - strand at 10.150 kb on - strand at 10.150 kb on - strand at 10.150 kb on - strand at 10.150 kb on - strand at 10.150 kb on - strand at 10.150 kb on - strand at 10.150 kb on - strand at 10.150 kb on - strand at 10.150 kb on - strand at 10.364 kb on + strand, within GFF7380 at 10.364 kb on + strand, within GFF7380 at 10.364 kb on + strand, within GFF7380 at 10.364 kb on + strand, within GFF7380 at 10.364 kb on + strand, within GFF7380 at 10.365 kb on - strand, within GFF7380 at 10.365 kb on - strand, within GFF7380 at 10.403 kb on + strand, within GFF7380 at 10.403 kb on + strand, within GFF7380 at 10.403 kb on + strand, within GFF7380 at 10.403 kb on + strand, within GFF7380 at 10.403 kb on + strand, within GFF7380 at 10.403 kb on + strand, within GFF7380 at 10.403 kb on + strand, within GFF7380 at 10.404 kb on - strand, within GFF7380 at 10.404 kb on - strand, within GFF7380 at 10.431 kb on - strand, within GFF7380 at 10.431 kb on - strand, within GFF7380 at 10.431 kb on - strand, within GFF7380 at 10.431 kb on - strand, within GFF7380 at 10.431 kb on - strand, within GFF7380 at 10.442 kb on + strand, within GFF7380 at 10.442 kb on + strand, within GFF7380 at 10.442 kb on + strand, within GFF7380 at 10.442 kb on + strand, within GFF7380 at 10.442 kb on + strand, within GFF7380 at 10.443 kb on - strand, within GFF7380 at 10.443 kb on - strand, within GFF7380 at 10.590 kb on - strand, within GFF7380 at 10.865 kb on + strand, within GFF7380 at 10.866 kb on - strand, within GFF7380 at 10.866 kb on - strand, within GFF7380 at 11.028 kb on + strand, within GFF7380 at 11.028 kb on + strand, within GFF7380 at 11.029 kb on - strand, within GFF7380 at 11.029 kb on - strand, within GFF7380 at 11.029 kb on - strand, within GFF7380 at 11.029 kb on - strand, within GFF7380 at 11.066 kb on + strand, within GFF7380 at 11.067 kb on - strand, within GFF7380 at 11.067 kb on - strand, within GFF7380 at 11.067 kb on - strand, within GFF7380 at 11.067 kb on - strand, within GFF7380 at 11.067 kb on - strand, within GFF7380 at 11.067 kb on - strand, within GFF7380
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 8,198 + GFF7377 0.76 -0.5 8,198 + GFF7377 0.76 +0.2 8,199 - GFF7377 0.76 -1.8 8,199 - GFF7377 0.76 -0.2 8,199 - GFF7377 0.76 +0.5 8,199 - GFF7377 0.76 +0.8 8,199 - GFF7377 0.76 -1.1 8,199 - GFF7377 0.76 +0.6 8,474 + +0.1 8,475 - -0.1 8,475 - -0.2 8,475 - +0.1 8,554 + -0.9 8,554 + +1.2 8,554 + -0.1 8,554 + +0.4 8,554 + +0.3 8,554 + +0.8 8,555 - +2.5 8,555 - -0.6 8,555 - +0.3 8,638 + GFF7378 0.20 +0.1 8,740 + GFF7378 0.46 +2.4 9,111 + GFF7379 0.14 +0.2 9,111 + GFF7379 0.14 +0.8 9,111 + GFF7379 0.14 -0.4 9,111 + GFF7379 0.14 -0.4 9,111 + GFF7379 0.14 +1.2 9,111 + GFF7379 0.14 -1.0 9,111 + GFF7379 0.14 +0.1 9,111 + GFF7379 0.14 +0.7 9,111 + GFF7379 0.14 -0.8 9,112 - GFF7379 0.14 +1.4 9,112 - GFF7379 0.14 -1.7 9,112 - GFF7379 0.14 -0.2 9,112 - GFF7379 0.14 +0.4 9,112 - GFF7379 0.14 -0.1 9,112 - GFF7379 0.14 +0.1 9,112 - GFF7379 0.14 -0.4 9,112 - GFF7379 0.14 +1.1 9,112 - GFF7379 0.14 -0.1 9,112 - GFF7379 0.14 -0.4 9,112 - GFF7379 0.14 -0.7 9,316 - GFF7379 0.32 +1.8 9,316 - GFF7379 0.32 +0.0 9,316 - GFF7379 0.32 +2.0 9,316 - GFF7379 0.32 -0.2 9,316 - GFF7379 0.32 +0.7 9,316 - GFF7379 0.32 -0.6 9,316 - GFF7379 0.32 -0.4 9,316 - GFF7379 0.32 -0.2 9,390 + GFF7379 0.38 +1.9 9,390 + GFF7379 0.38 -0.8 9,390 + GFF7379 0.38 +1.1 9,390 + GFF7379 0.38 +0.9 9,390 + GFF7379 0.38 -1.0 9,390 + GFF7379 0.38 -0.3 9,390 + GFF7379 0.38 -0.5 9,391 - GFF7379 0.38 +0.4 9,391 - GFF7379 0.38 -0.2 9,391 - GFF7379 0.38 -0.5 9,391 - GFF7379 0.38 +0.6 9,391 - GFF7379 0.38 +0.9 9,391 - GFF7379 0.38 -0.2 9,391 - GFF7379 0.38 -0.5 9,391 - GFF7379 0.38 +0.6 9,528 + GFF7379 0.50 -1.7 9,529 - GFF7379 0.50 +0.3 9,529 - GFF7379 0.50 -0.6 9,529 - GFF7379 0.50 -1.5 9,735 + GFF7379 0.68 -0.2 9,736 - GFF7379 0.68 -0.3 9,736 - GFF7379 0.68 +1.8 9,736 - GFF7379 0.68 +0.5 9,891 + GFF7379 0.81 +0.2 9,891 + GFF7379 0.81 +0.5 9,891 + GFF7379 0.81 +0.4 9,891 + GFF7379 0.81 -0.9 9,891 + GFF7379 0.81 -0.7 9,891 + GFF7379 0.81 +1.8 9,891 + GFF7379 0.81 +0.6 9,891 + GFF7379 0.81 -1.3 9,891 + GFF7379 0.81 +1.0 9,891 + GFF7379 0.81 -0.6 9,892 - GFF7379 0.81 +0.9 9,892 - GFF7379 0.81 +0.1 9,892 - GFF7379 0.81 -0.8 9,892 - GFF7379 0.81 -0.1 9,892 - GFF7379 0.81 +0.1 9,892 - GFF7379 0.81 +0.1 9,892 - GFF7379 0.81 +0.3 9,892 - GFF7379 0.81 +1.9 9,892 - GFF7379 0.81 -1.5 9,892 - GFF7379 0.81 -0.2 9,892 - GFF7379 0.81 +0.8 9,892 - GFF7379 0.81 +1.4 9,892 - GFF7379 0.81 +0.2 9,975 + GFF7379 0.89 -0.2 9,976 - GFF7379 0.89 -2.2 9,976 - GFF7379 0.89 +0.0 9,976 - GFF7379 0.89 -0.5 10,149 + +0.3 10,149 + -0.5 10,149 + -0.4 10,149 + -0.3 10,149 + +0.0 10,149 + -1.2 10,149 + -0.5 10,149 + -1.0 10,149 + +0.0 10,149 + -0.1 10,149 + -0.1 10,149 + +0.6 10,149 + -0.7 10,149 + +0.9 10,149 + +1.8 10,150 - +0.3 10,150 - -1.4 10,150 - +0.5 10,150 - -0.5 10,150 - +0.2 10,150 - -0.2 10,150 - -0.7 10,150 - -1.2 10,150 - -0.7 10,150 - +0.6 10,150 - +0.2 10,150 - -0.7 10,150 - +0.6 10,150 - -0.1 10,150 - +0.1 10,150 - +0.2 10,150 - -1.1 10,150 - +1.1 10,150 - -1.9 10,150 - +0.4 10,364 + GFF7380 0.20 -1.8 10,364 + GFF7380 0.20 -2.1 10,364 + GFF7380 0.20 -1.9 10,364 + GFF7380 0.20 -1.3 10,364 + GFF7380 0.20 +0.0 10,365 - GFF7380 0.21 -1.7 10,365 - GFF7380 0.21 -0.7 10,403 + GFF7380 0.24 -0.5 10,403 + GFF7380 0.24 -1.7 10,403 + GFF7380 0.24 -0.4 10,403 + GFF7380 0.24 -1.2 10,403 + GFF7380 0.24 +0.9 10,403 + GFF7380 0.24 -1.7 10,403 + GFF7380 0.24 -2.7 10,404 - GFF7380 0.24 -0.8 10,404 - GFF7380 0.24 -0.5 10,431 - GFF7380 0.26 +0.2 10,431 - GFF7380 0.26 +0.5 10,431 - GFF7380 0.26 -0.5 10,431 - GFF7380 0.26 -1.8 10,431 - GFF7380 0.26 -0.2 10,442 + GFF7380 0.27 -0.6 10,442 + GFF7380 0.27 -2.0 10,442 + GFF7380 0.27 -0.6 10,442 + GFF7380 0.27 +0.2 10,442 + GFF7380 0.27 -2.2 10,443 - GFF7380 0.28 -0.3 10,443 - GFF7380 0.28 -0.6 10,590 - GFF7380 0.41 -0.9 10,865 + GFF7380 0.65 -2.4 10,866 - GFF7380 0.65 +0.0 10,866 - GFF7380 0.65 -0.5 11,028 + GFF7380 0.80 -1.8 11,028 + GFF7380 0.80 -2.8 11,029 - GFF7380 0.80 +0.2 11,029 - GFF7380 0.80 -1.6 11,029 - GFF7380 0.80 -1.5 11,029 - GFF7380 0.80 -0.8 11,066 + GFF7380 0.83 -0.7 11,067 - GFF7380 0.83 -0.8 11,067 - GFF7380 0.83 -0.5 11,067 - GFF7380 0.83 -0.2 11,067 - GFF7380 0.83 -0.7 11,067 - GFF7380 0.83 -1.5 11,067 - GFF7380 0.83 -0.7
Or see this region's nucleotide sequence