Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF7259 and GFF7260 are separated by 30 nucleotides GFF7260 and GFF7261 are separated by 2 nucleotides GFF7261 and GFF7262 overlap by 4 nucleotides GFF7262 and GFF7263 are separated by 6 nucleotides
GFF7259 - no description, at 6,685 to 7,509
GFF7259
GFF7260 - hypothetical protein, at 7,540 to 7,989
GFF7260
GFF7261 - 2,5-dioxovalerate dehydrogenase (EC 1.2.1.26), at 7,992 to 9,428
GFF7261
GFF7262 - no description, at 9,425 to 9,718
GFF7262
GFF7263 - Malate dehydrogenase (EC 1.1.1.37), at 9,725 to 10,819
GFF7263
Position (kb) 7
8
9
10 Strain fitness (log2 ratio) -2
-1
0
1
2 at 7.125 kb on + strand, within GFF7259 at 7.207 kb on + strand, within GFF7259 at 7.207 kb on + strand, within GFF7259 at 7.208 kb on - strand, within GFF7259 at 7.208 kb on - strand, within GFF7259 at 7.261 kb on + strand, within GFF7259 at 7.551 kb on + strand at 7.552 kb on - strand at 7.555 kb on + strand at 7.859 kb on - strand, within GFF7260 at 7.900 kb on + strand, within GFF7260 at 7.901 kb on - strand, within GFF7260 at 7.901 kb on - strand, within GFF7260 at 7.901 kb on - strand, within GFF7260 at 8.333 kb on + strand, within GFF7261 at 9.590 kb on + strand, within GFF7262 at 9.590 kb on + strand, within GFF7262 at 9.635 kb on + strand, within GFF7262 at 9.683 kb on + strand, within GFF7262 at 9.683 kb on + strand, within GFF7262 at 9.707 kb on + strand at 9.761 kb on + strand at 9.860 kb on + strand, within GFF7263 at 10.047 kb on - strand, within GFF7263 at 10.164 kb on - strand, within GFF7263 at 10.226 kb on - strand, within GFF7263
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 7,125 + GFF7259 0.53 -0.0 7,207 + GFF7259 0.63 +0.6 7,207 + GFF7259 0.63 -1.4 7,208 - GFF7259 0.63 -1.0 7,208 - GFF7259 0.63 -0.0 7,261 + GFF7259 0.70 +1.8 7,551 + -1.5 7,552 - -0.1 7,555 + -1.0 7,859 - GFF7260 0.71 -0.4 7,900 + GFF7260 0.80 -0.0 7,901 - GFF7260 0.80 +0.0 7,901 - GFF7260 0.80 +0.5 7,901 - GFF7260 0.80 -1.0 8,333 + GFF7261 0.24 -1.1 9,590 + GFF7262 0.56 -0.6 9,590 + GFF7262 0.56 +0.7 9,635 + GFF7262 0.71 -0.4 9,683 + GFF7262 0.88 -0.6 9,683 + GFF7262 0.88 -0.5 9,707 + +0.2 9,761 + -1.9 9,860 + GFF7263 0.12 +0.2 10,047 - GFF7263 0.29 -1.2 10,164 - GFF7263 0.40 -1.8 10,226 - GFF7263 0.46 -1.0
Or see this region's nucleotide sequence