Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6995 and GFF6996 overlap by 4 nucleotides GFF6996 and GFF6997 overlap by 4 nucleotides GFF6997 and GFF6998 are separated by 1 nucleotides GFF6998 and GFF6999 are separated by 46 nucleotides
GFF6995 - Flagellar M-ring protein FliF, at 12,387 to 13,901
GFF6995
GFF6996 - Flagellar hook-basal body complex protein FliE, at 13,898 to 14,263
GFF6996
GFF6997 - Flagellar basal-body rod protein FlgC, at 14,260 to 14,709
GFF6997
GFF6998 - Flagellar basal-body rod protein FlgB, at 14,711 to 15,094
GFF6998
GFF6999 - Flagellar P-ring protein FlgI, at 15,141 to 16,277
GFF6999
Position (kb) 14
15 Strain fitness (log2 ratio) -1
0
1 at 13.880 kb on + strand at 13.880 kb on + strand at 13.901 kb on + strand at 13.915 kb on + strand at 14.048 kb on + strand, within GFF6996 at 14.337 kb on - strand, within GFF6997 at 14.439 kb on - strand, within GFF6997 at 14.702 kb on + strand at 14.915 kb on + strand, within GFF6998 at 14.979 kb on + strand, within GFF6998
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 13,880 + -1.3 13,880 + +0.4 13,901 + -0.8 13,915 + +0.5 14,048 + GFF6996 0.41 -0.2 14,337 - GFF6997 0.17 +0.7 14,439 - GFF6997 0.40 +0.0 14,702 + -0.9 14,915 + GFF6998 0.53 +0.1 14,979 + GFF6998 0.70 +0.3
Or see this region's nucleotide sequence