Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6640 and GFF6641 are separated by 12 nucleotides GFF6641 and GFF6642 are separated by 13 nucleotides GFF6642 and GFF6643 are separated by 4 nucleotides
GFF6640 - ABC transporter, ATP-binding protein (cluster 5, nickel/peptides/opines) / ABC transporter, ATP-binding protein (cluster 5, nickel/peptides/opines), at 12,792 to 14,429
GFF6640
GFF6641 - ABC transporter, permease protein 2 (cluster 5, nickel/peptides/opines), at 14,442 to 15,314
GFF6641
GFF6642 - ABC transporter, permease protein 1 (cluster 5, nickel/peptides/opines), at 15,328 to 16,269
GFF6642
GFF6643 - ABC transporter, substrate-binding protein (cluster 5, nickel/peptides/opines), at 16,274 to 17,884
GFF6643
Position (kb)
15
16
17 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 14.974 kb on + strand, within GFF6641 at 14.975 kb on - strand, within GFF6641 at 15.061 kb on + strand, within GFF6641 at 15.078 kb on + strand, within GFF6641 at 15.078 kb on + strand, within GFF6641 at 15.079 kb on - strand, within GFF6641 at 15.079 kb on - strand, within GFF6641 at 15.079 kb on - strand, within GFF6641 at 15.079 kb on - strand, within GFF6641 at 15.332 kb on + strand at 15.359 kb on + strand at 15.359 kb on + strand at 15.359 kb on + strand at 15.359 kb on + strand at 15.359 kb on + strand at 15.359 kb on + strand at 15.359 kb on + strand at 15.360 kb on - strand at 15.360 kb on - strand at 15.360 kb on - strand at 15.360 kb on - strand at 15.360 kb on - strand at 15.360 kb on - strand at 15.360 kb on - strand at 15.360 kb on - strand at 15.392 kb on + strand at 15.392 kb on + strand at 15.392 kb on + strand at 15.392 kb on + strand at 15.392 kb on + strand at 15.392 kb on + strand at 15.392 kb on + strand at 15.392 kb on + strand at 15.393 kb on - strand at 15.393 kb on - strand at 15.393 kb on - strand at 15.393 kb on - strand at 15.393 kb on - strand at 15.393 kb on - strand at 15.695 kb on + strand, within GFF6642 at 15.695 kb on + strand, within GFF6642 at 15.695 kb on + strand, within GFF6642 at 15.695 kb on + strand, within GFF6642 at 15.695 kb on + strand, within GFF6642 at 15.695 kb on + strand, within GFF6642 at 15.695 kb on + strand, within GFF6642 at 15.695 kb on + strand, within GFF6642 at 15.695 kb on + strand, within GFF6642 at 15.695 kb on + strand, within GFF6642 at 15.696 kb on - strand, within GFF6642 at 15.696 kb on - strand, within GFF6642 at 15.696 kb on - strand, within GFF6642 at 15.696 kb on - strand, within GFF6642 at 15.696 kb on - strand, within GFF6642 at 15.696 kb on - strand, within GFF6642 at 15.696 kb on - strand, within GFF6642 at 15.770 kb on + strand, within GFF6642 at 15.771 kb on - strand, within GFF6642 at 15.812 kb on + strand, within GFF6642 at 15.812 kb on + strand, within GFF6642 at 15.812 kb on + strand, within GFF6642 at 15.813 kb on + strand, within GFF6642 at 15.813 kb on - strand, within GFF6642 at 15.813 kb on - strand, within GFF6642 at 15.824 kb on + strand, within GFF6642 at 15.825 kb on - strand, within GFF6642 at 16.275 kb on + strand at 16.275 kb on + strand at 16.275 kb on + strand at 16.275 kb on + strand at 16.275 kb on + strand at 16.296 kb on + strand at 16.296 kb on + strand at 16.296 kb on + strand at 16.296 kb on + strand at 16.296 kb on + strand at 16.297 kb on - strand at 16.359 kb on + strand at 16.360 kb on - strand at 16.360 kb on - strand at 16.360 kb on - strand at 16.767 kb on + strand, within GFF6643 at 16.768 kb on - strand, within GFF6643 at 16.768 kb on - strand, within GFF6643 at 16.821 kb on + strand, within GFF6643 at 16.821 kb on + strand, within GFF6643 at 16.821 kb on + strand, within GFF6643 at 16.821 kb on + strand, within GFF6643 at 16.822 kb on - strand, within GFF6643 at 16.822 kb on - strand, within GFF6643 at 17.067 kb on + strand, within GFF6643 at 17.068 kb on - strand, within GFF6643 at 17.068 kb on - strand, within GFF6643 at 17.068 kb on - strand, within GFF6643 at 17.068 kb on - strand, within GFF6643 at 17.172 kb on + strand, within GFF6643 at 17.172 kb on + strand, within GFF6643 at 17.173 kb on - strand, within GFF6643 at 17.173 kb on - strand, within GFF6643 at 17.173 kb on - strand, within GFF6643 at 17.173 kb on - strand, within GFF6643 at 17.173 kb on - strand, within GFF6643 at 17.223 kb on + strand, within GFF6643 at 17.223 kb on + strand, within GFF6643 at 17.223 kb on + strand, within GFF6643 at 17.223 kb on + strand, within GFF6643 at 17.223 kb on + strand, within GFF6643 at 17.224 kb on - strand, within GFF6643 at 17.224 kb on - strand, within GFF6643
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 14,974 + GFF6641 0.61 -0.6 14,975 - GFF6641 0.61 -0.4 15,061 + GFF6641 0.71 +1.0 15,078 + GFF6641 0.73 -0.0 15,078 + GFF6641 0.73 -1.4 15,079 - GFF6641 0.73 +0.1 15,079 - GFF6641 0.73 -1.2 15,079 - GFF6641 0.73 -1.2 15,079 - GFF6641 0.73 +0.5 15,332 + +0.3 15,359 + +0.4 15,359 + +0.2 15,359 + -1.0 15,359 + -0.0 15,359 + +0.2 15,359 + -1.0 15,359 + -0.3 15,360 - +0.2 15,360 - -0.0 15,360 - -1.4 15,360 - -0.9 15,360 - -0.0 15,360 - -3.2 15,360 - -1.4 15,360 - -1.2 15,392 + +0.1 15,392 + +0.1 15,392 + +0.6 15,392 + +0.3 15,392 + +0.6 15,392 + -0.4 15,392 + +0.2 15,392 + -0.5 15,393 - -0.7 15,393 - -0.0 15,393 - +0.6 15,393 - -0.3 15,393 - -2.2 15,393 - -0.9 15,695 + GFF6642 0.39 -1.2 15,695 + GFF6642 0.39 -1.4 15,695 + GFF6642 0.39 -0.4 15,695 + GFF6642 0.39 -2.6 15,695 + GFF6642 0.39 -0.0 15,695 + GFF6642 0.39 -0.2 15,695 + GFF6642 0.39 -0.0 15,695 + GFF6642 0.39 -1.1 15,695 + GFF6642 0.39 -1.1 15,695 + GFF6642 0.39 -3.4 15,696 - GFF6642 0.39 -1.3 15,696 - GFF6642 0.39 -1.3 15,696 - GFF6642 0.39 -0.0 15,696 - GFF6642 0.39 -0.7 15,696 - GFF6642 0.39 +0.8 15,696 - GFF6642 0.39 -1.0 15,696 - GFF6642 0.39 -0.8 15,770 + GFF6642 0.47 +0.8 15,771 - GFF6642 0.47 -1.5 15,812 + GFF6642 0.51 +0.4 15,812 + GFF6642 0.51 -1.4 15,812 + GFF6642 0.51 -0.3 15,813 + GFF6642 0.51 -1.0 15,813 - GFF6642 0.51 +0.4 15,813 - GFF6642 0.51 +0.7 15,824 + GFF6642 0.53 +0.2 15,825 - GFF6642 0.53 -0.1 16,275 + -1.2 16,275 + +0.1 16,275 + +0.8 16,275 + -0.6 16,275 + -0.1 16,296 + -0.8 16,296 + -0.2 16,296 + +0.8 16,296 + -0.8 16,296 + -1.2 16,297 - +0.6 16,359 + -0.2 16,360 - +0.6 16,360 - +0.4 16,360 - -0.8 16,767 + GFF6643 0.31 -0.7 16,768 - GFF6643 0.31 -0.2 16,768 - GFF6643 0.31 +0.6 16,821 + GFF6643 0.34 -1.8 16,821 + GFF6643 0.34 -0.2 16,821 + GFF6643 0.34 -0.3 16,821 + GFF6643 0.34 -0.3 16,822 - GFF6643 0.34 +0.8 16,822 - GFF6643 0.34 +0.3 17,067 + GFF6643 0.49 -0.7 17,068 - GFF6643 0.49 -0.9 17,068 - GFF6643 0.49 -1.0 17,068 - GFF6643 0.49 -0.4 17,068 - GFF6643 0.49 -0.0 17,172 + GFF6643 0.56 +0.2 17,172 + GFF6643 0.56 -0.0 17,173 - GFF6643 0.56 +0.8 17,173 - GFF6643 0.56 -3.0 17,173 - GFF6643 0.56 -1.1 17,173 - GFF6643 0.56 -1.0 17,173 - GFF6643 0.56 +0.4 17,223 + GFF6643 0.59 -1.1 17,223 + GFF6643 0.59 -1.2 17,223 + GFF6643 0.59 +0.8 17,223 + GFF6643 0.59 -0.8 17,223 + GFF6643 0.59 -0.5 17,224 - GFF6643 0.59 -1.7 17,224 - GFF6643 0.59 -1.0
Or see this region's nucleotide sequence