Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6585 and GFF6586 overlap by 4 nucleotides GFF6586 and GFF6587 are separated by 30 nucleotides GFF6587 and GFF6588 are separated by 85 nucleotides
GFF6585 - D-glycerate 2-kinase (EC 2.7.1.165), at 26,854 to 28,179
GFF6585
GFF6586 - 2-hydroxy-3-oxopropionate reductase (EC 1.1.1.60), at 28,176 to 29,084
GFF6586
GFF6587 - Hydroxypyruvate isomerase (EC 5.3.1.22), at 29,115 to 29,894
GFF6587
GFF6588 - Glyoxylate carboligase (EC 4.1.1.47), at 29,980 to 31,770
GFF6588
Position (kb) 29
30 Strain fitness (log2 ratio) -2
-1
0
1 at 28.641 kb on + strand, within GFF6586 at 28.642 kb on - strand, within GFF6586 at 28.723 kb on + strand, within GFF6586 at 28.723 kb on + strand, within GFF6586 at 28.979 kb on - strand, within GFF6586 at 28.979 kb on - strand, within GFF6586 at 29.086 kb on + strand at 29.086 kb on + strand at 29.086 kb on + strand at 29.086 kb on + strand at 29.087 kb on - strand at 29.087 kb on - strand at 29.087 kb on - strand at 29.087 kb on - strand at 29.087 kb on - strand at 29.116 kb on + strand at 29.117 kb on - strand at 29.173 kb on + strand at 29.174 kb on - strand at 29.309 kb on - strand, within GFF6587 at 29.309 kb on - strand, within GFF6587 at 29.309 kb on - strand, within GFF6587 at 29.356 kb on + strand, within GFF6587 at 29.356 kb on + strand, within GFF6587 at 29.356 kb on + strand, within GFF6587 at 29.356 kb on + strand, within GFF6587 at 29.356 kb on + strand, within GFF6587 at 29.356 kb on + strand, within GFF6587 at 29.356 kb on + strand, within GFF6587 at 29.357 kb on - strand, within GFF6587 at 29.357 kb on - strand, within GFF6587 at 29.357 kb on - strand, within GFF6587 at 29.365 kb on + strand, within GFF6587 at 29.365 kb on + strand, within GFF6587 at 29.366 kb on - strand, within GFF6587 at 29.434 kb on + strand, within GFF6587 at 29.434 kb on + strand, within GFF6587 at 29.434 kb on + strand, within GFF6587 at 29.435 kb on - strand, within GFF6587 at 29.435 kb on - strand, within GFF6587 at 29.676 kb on + strand, within GFF6587 at 29.676 kb on + strand, within GFF6587 at 29.677 kb on - strand, within GFF6587 at 29.901 kb on + strand at 29.901 kb on + strand at 29.902 kb on - strand at 29.907 kb on + strand at 30.302 kb on + strand, within GFF6588 at 30.302 kb on + strand, within GFF6588 at 30.302 kb on + strand, within GFF6588 at 30.302 kb on + strand, within GFF6588 at 30.303 kb on - strand, within GFF6588 at 30.303 kb on - strand, within GFF6588 at 30.303 kb on - strand, within GFF6588 at 30.303 kb on - strand, within GFF6588 at 30.374 kb on + strand, within GFF6588 at 30.551 kb on + strand, within GFF6588 at 30.551 kb on + strand, within GFF6588 at 30.552 kb on - strand, within GFF6588 at 30.605 kb on + strand, within GFF6588 at 30.667 kb on + strand, within GFF6588 at 30.667 kb on + strand, within GFF6588 at 30.667 kb on + strand, within GFF6588 at 30.737 kb on + strand, within GFF6588 at 30.738 kb on - strand, within GFF6588
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 28,641 + GFF6586 0.51 -2.3 28,642 - GFF6586 0.51 -0.2 28,723 + GFF6586 0.60 +0.7 28,723 + GFF6586 0.60 -0.5 28,979 - GFF6586 0.88 +0.1 28,979 - GFF6586 0.88 +0.5 29,086 + -0.1 29,086 + -1.7 29,086 + -0.6 29,086 + -0.4 29,087 - +1.6 29,087 - -0.3 29,087 - +0.3 29,087 - -0.1 29,087 - +1.3 29,116 + +1.6 29,117 - +0.6 29,173 + -0.3 29,174 - -0.4 29,309 - GFF6587 0.25 -0.4 29,309 - GFF6587 0.25 +1.1 29,309 - GFF6587 0.25 +0.2 29,356 + GFF6587 0.31 -1.0 29,356 + GFF6587 0.31 -0.7 29,356 + GFF6587 0.31 -0.3 29,356 + GFF6587 0.31 -0.4 29,356 + GFF6587 0.31 -0.9 29,356 + GFF6587 0.31 -0.1 29,356 + GFF6587 0.31 -2.1 29,357 - GFF6587 0.31 -0.7 29,357 - GFF6587 0.31 -1.6 29,357 - GFF6587 0.31 -1.8 29,365 + GFF6587 0.32 -1.6 29,365 + GFF6587 0.32 -0.1 29,366 - GFF6587 0.32 -1.5 29,434 + GFF6587 0.41 +0.2 29,434 + GFF6587 0.41 -1.2 29,434 + GFF6587 0.41 -0.8 29,435 - GFF6587 0.41 +1.2 29,435 - GFF6587 0.41 -0.1 29,676 + GFF6587 0.72 -0.4 29,676 + GFF6587 0.72 -0.7 29,677 - GFF6587 0.72 +1.6 29,901 + +0.0 29,901 + +0.6 29,902 - -0.1 29,907 + -1.3 30,302 + GFF6588 0.18 +1.2 30,302 + GFF6588 0.18 +0.6 30,302 + GFF6588 0.18 -0.4 30,302 + GFF6588 0.18 +0.0 30,303 - GFF6588 0.18 -1.3 30,303 - GFF6588 0.18 -1.7 30,303 - GFF6588 0.18 +0.7 30,303 - GFF6588 0.18 -1.5 30,374 + GFF6588 0.22 +0.3 30,551 + GFF6588 0.32 -0.4 30,551 + GFF6588 0.32 -0.1 30,552 - GFF6588 0.32 -1.6 30,605 + GFF6588 0.35 -0.5 30,667 + GFF6588 0.38 -0.4 30,667 + GFF6588 0.38 +0.1 30,667 + GFF6588 0.38 -1.0 30,737 + GFF6588 0.42 -0.1 30,738 - GFF6588 0.42 -0.5
Or see this region's nucleotide sequence