Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6572 and GFF6573 overlap by 4 nucleotides GFF6573 and GFF6574 are separated by 12 nucleotides GFF6574 and GFF6575 are separated by 12 nucleotides GFF6575 and GFF6576 overlap by 4 nucleotides
GFF6572 - Conjugative transfer protein TrbG, at 13,220 to 14,227
GFF6572
GFF6573 - Conjugative transfer protein TrbF, at 14,224 to 14,928
GFF6573
GFF6574 - Conjugative transfer protein TrbL, at 14,941 to 16,266
GFF6574
GFF6575 - Conjugative transfer protein TrbJ, at 16,279 to 17,049
GFF6575
GFF6576 - Conjugative transfer protein TrbE, at 17,046 to 19,520
GFF6576
Position (kb)
14
15
16
17 Strain fitness (log2 ratio)
-2
-1
0
1 at 14.209 kb on + strand at 14.210 kb on - strand at 14.210 kb on - strand at 14.210 kb on - strand at 14.282 kb on + strand at 14.282 kb on + strand at 14.282 kb on + strand at 14.282 kb on + strand at 14.282 kb on + strand at 14.282 kb on + strand at 14.282 kb on + strand at 14.282 kb on + strand at 14.283 kb on - strand at 14.283 kb on - strand at 14.283 kb on - strand at 14.550 kb on - strand, within GFF6573 at 14.550 kb on - strand, within GFF6573 at 14.631 kb on - strand, within GFF6573 at 14.631 kb on - strand, within GFF6573 at 14.718 kb on - strand, within GFF6573 at 14.718 kb on - strand, within GFF6573 at 14.718 kb on - strand, within GFF6573 at 14.718 kb on - strand, within GFF6573 at 16.040 kb on + strand, within GFF6574 at 16.040 kb on + strand, within GFF6574 at 16.040 kb on + strand, within GFF6574 at 16.041 kb on - strand, within GFF6574 at 16.058 kb on + strand, within GFF6574 at 16.058 kb on + strand, within GFF6574 at 16.058 kb on + strand, within GFF6574 at 16.059 kb on - strand, within GFF6574 at 16.059 kb on - strand, within GFF6574 at 16.059 kb on - strand, within GFF6574 at 16.146 kb on + strand at 16.147 kb on - strand at 16.147 kb on - strand at 16.147 kb on - strand at 16.147 kb on - strand at 16.147 kb on - strand at 16.147 kb on - strand at 16.217 kb on + strand at 16.218 kb on - strand at 16.218 kb on - strand at 16.290 kb on - strand at 16.313 kb on + strand at 16.313 kb on + strand at 16.313 kb on + strand at 16.314 kb on - strand at 16.314 kb on - strand at 16.314 kb on - strand at 16.668 kb on - strand, within GFF6575 at 16.668 kb on - strand, within GFF6575 at 16.668 kb on - strand, within GFF6575 at 16.679 kb on + strand, within GFF6575 at 16.680 kb on - strand, within GFF6575 at 16.718 kb on + strand, within GFF6575 at 16.849 kb on + strand, within GFF6575 at 16.849 kb on + strand, within GFF6575 at 16.850 kb on - strand, within GFF6575 at 16.850 kb on - strand, within GFF6575
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 14,209 + +0.6 14,210 - -1.3 14,210 - +0.3 14,210 - +0.6 14,282 + +0.6 14,282 + -0.3 14,282 + -0.7 14,282 + -1.1 14,282 + -0.3 14,282 + -0.4 14,282 + +0.4 14,282 + -0.0 14,283 - -1.4 14,283 - -1.2 14,283 - -0.1 14,550 - GFF6573 0.46 -0.3 14,550 - GFF6573 0.46 -0.7 14,631 - GFF6573 0.58 -0.9 14,631 - GFF6573 0.58 +0.4 14,718 - GFF6573 0.70 +0.1 14,718 - GFF6573 0.70 -0.2 14,718 - GFF6573 0.70 +0.2 14,718 - GFF6573 0.70 -0.5 16,040 + GFF6574 0.83 -0.2 16,040 + GFF6574 0.83 -1.4 16,040 + GFF6574 0.83 +1.0 16,041 - GFF6574 0.83 -0.2 16,058 + GFF6574 0.84 -1.1 16,058 + GFF6574 0.84 -1.1 16,058 + GFF6574 0.84 -0.6 16,059 - GFF6574 0.84 +0.6 16,059 - GFF6574 0.84 -1.4 16,059 - GFF6574 0.84 -0.7 16,146 + -0.1 16,147 - -0.7 16,147 - +0.1 16,147 - -0.2 16,147 - -0.3 16,147 - -2.3 16,147 - -1.3 16,217 + -0.2 16,218 - +0.0 16,218 - +0.1 16,290 - +1.3 16,313 + +0.8 16,313 + -0.1 16,313 + -0.6 16,314 - +1.3 16,314 - -0.2 16,314 - -0.8 16,668 - GFF6575 0.50 +1.4 16,668 - GFF6575 0.50 +1.2 16,668 - GFF6575 0.50 +0.4 16,679 + GFF6575 0.52 +0.0 16,680 - GFF6575 0.52 +0.6 16,718 + GFF6575 0.57 +0.0 16,849 + GFF6575 0.74 -0.4 16,849 + GFF6575 0.74 +1.0 16,850 - GFF6575 0.74 -0.3 16,850 - GFF6575 0.74 +0.3
Or see this region's nucleotide sequence