Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6541 and GFF6542 are separated by 183 nucleotides GFF6542 and GFF6543 overlap by 4 nucleotides
GFF6541 - Oxidoreductase, short-chain dehydrogenase/reductase family, at 38,459 to 39,262
GFF6541
GFF6542 - Transcriptional regulator, AcrR family, at 39,446 to 40,162
GFF6542
GFF6543 - Transcriptional regulator, LysR family, at 40,159 to 41,133
GFF6543
Position (kb) 39
40
41 Strain fitness (log2 ratio) -2
-1
0
1 at 38.688 kb on + strand, within GFF6541 at 39.280 kb on + strand at 39.281 kb on - strand at 39.307 kb on + strand at 39.410 kb on + strand at 39.411 kb on - strand at 39.411 kb on - strand at 39.411 kb on - strand at 39.432 kb on + strand at 39.433 kb on - strand at 39.590 kb on + strand, within GFF6542 at 39.591 kb on - strand, within GFF6542 at 39.654 kb on - strand, within GFF6542 at 39.654 kb on - strand, within GFF6542 at 39.656 kb on + strand, within GFF6542 at 39.656 kb on + strand, within GFF6542 at 39.656 kb on + strand, within GFF6542 at 39.656 kb on + strand, within GFF6542 at 39.656 kb on + strand, within GFF6542 at 39.701 kb on + strand, within GFF6542 at 39.702 kb on - strand, within GFF6542 at 39.702 kb on - strand, within GFF6542 at 39.725 kb on + strand, within GFF6542 at 39.725 kb on + strand, within GFF6542 at 39.726 kb on - strand, within GFF6542 at 39.726 kb on - strand, within GFF6542 at 39.834 kb on - strand, within GFF6542 at 39.983 kb on + strand, within GFF6542 at 39.984 kb on - strand, within GFF6542 at 39.984 kb on - strand, within GFF6542 at 40.160 kb on + strand at 40.448 kb on + strand, within GFF6543 at 40.934 kb on + strand, within GFF6543 at 40.934 kb on + strand, within GFF6543 at 40.935 kb on - strand, within GFF6543 at 40.941 kb on - strand, within GFF6543 at 41.140 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 38,688 + GFF6541 0.28 +1.6 39,280 + +1.2 39,281 - +0.3 39,307 + -2.0 39,410 + +0.1 39,411 - -0.3 39,411 - +0.4 39,411 - +1.6 39,432 + +0.6 39,433 - +0.3 39,590 + GFF6542 0.20 -0.4 39,591 - GFF6542 0.20 -0.9 39,654 - GFF6542 0.29 +0.5 39,654 - GFF6542 0.29 -0.6 39,656 + GFF6542 0.29 -1.3 39,656 + GFF6542 0.29 +0.5 39,656 + GFF6542 0.29 -1.6 39,656 + GFF6542 0.29 -0.0 39,656 + GFF6542 0.29 +0.2 39,701 + GFF6542 0.36 +0.8 39,702 - GFF6542 0.36 -0.1 39,702 - GFF6542 0.36 +1.2 39,725 + GFF6542 0.39 +0.5 39,725 + GFF6542 0.39 +1.0 39,726 - GFF6542 0.39 -0.2 39,726 - GFF6542 0.39 +1.2 39,834 - GFF6542 0.54 -0.4 39,983 + GFF6542 0.75 -0.8 39,984 - GFF6542 0.75 -0.1 39,984 - GFF6542 0.75 +0.6 40,160 + -0.8 40,448 + GFF6543 0.30 -0.1 40,934 + GFF6543 0.79 -0.8 40,934 + GFF6543 0.79 +0.2 40,935 - GFF6543 0.80 -1.1 40,941 - GFF6543 0.80 +0.2 41,140 - -0.1
Or see this region's nucleotide sequence