Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6511 and GFF6512 are separated by 2 nucleotides GFF6512 and GFF6513 are separated by 159 nucleotides GFF6513 and GFF6514 are separated by 309 nucleotides
GFF6511 - Haloacid dehalogenase, type II (EC 3.8.1.2), at 6,982 to 7,710
GFF6511
GFF6512 - 4-hydroxy-2-oxoglutarate aldolase (EC 4.1.3.16) @ 2-dehydro-3-deoxyphosphogluconate aldolase (EC 4.1.2.14), at 7,713 to 8,348
GFF6512
GFF6513 - Phosphogluconate dehydratase (EC 4.2.1.12), at 8,508 to 10,340
GFF6513
GFF6514 - Short-chain dehydrogenase, at 10,650 to 11,471
GFF6514
Position (kb)
8
9
10
11 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 7.966 kb on + strand, within GFF6512 at 7.967 kb on - strand, within GFF6512 at 7.967 kb on - strand, within GFF6512 at 7.967 kb on - strand, within GFF6512 at 8.370 kb on + strand at 8.371 kb on - strand at 8.769 kb on + strand, within GFF6513 at 8.770 kb on - strand, within GFF6513 at 9.122 kb on + strand, within GFF6513 at 9.123 kb on - strand, within GFF6513 at 9.317 kb on - strand, within GFF6513 at 9.317 kb on - strand, within GFF6513 at 9.658 kb on + strand, within GFF6513 at 9.658 kb on + strand, within GFF6513 at 9.658 kb on + strand, within GFF6513 at 9.658 kb on + strand, within GFF6513 at 9.658 kb on + strand, within GFF6513 at 9.659 kb on - strand, within GFF6513 at 9.659 kb on - strand, within GFF6513 at 9.659 kb on - strand, within GFF6513 at 9.685 kb on + strand, within GFF6513 at 9.686 kb on - strand, within GFF6513 at 10.079 kb on - strand, within GFF6513 at 10.108 kb on + strand, within GFF6513 at 10.109 kb on - strand, within GFF6513 at 10.261 kb on + strand at 10.262 kb on - strand at 10.399 kb on + strand at 10.399 kb on + strand at 10.400 kb on - strand at 10.400 kb on - strand at 10.446 kb on - strand at 10.454 kb on - strand at 10.454 kb on - strand at 10.466 kb on + strand at 10.616 kb on + strand at 10.617 kb on - strand at 10.617 kb on - strand at 10.663 kb on + strand at 10.663 kb on + strand at 10.664 kb on - strand at 10.664 kb on - strand at 10.664 kb on - strand at 11.086 kb on - strand, within GFF6514 at 11.134 kb on - strand, within GFF6514 at 11.134 kb on - strand, within GFF6514 at 11.134 kb on - strand, within GFF6514 at 11.140 kb on + strand, within GFF6514 at 11.140 kb on + strand, within GFF6514 at 11.141 kb on - strand, within GFF6514 at 11.244 kb on + strand, within GFF6514 at 11.244 kb on + strand, within GFF6514 at 11.332 kb on - strand, within GFF6514 at 11.332 kb on - strand, within GFF6514 at 11.332 kb on - strand, within GFF6514
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 7,966 + GFF6512 0.40 -1.2 7,967 - GFF6512 0.40 -1.8 7,967 - GFF6512 0.40 -1.6 7,967 - GFF6512 0.40 -0.0 8,370 + -0.1 8,371 - -0.6 8,769 + GFF6513 0.14 -3.8 8,770 - GFF6513 0.14 -3.1 9,122 + GFF6513 0.33 +0.2 9,123 - GFF6513 0.34 -3.0 9,317 - GFF6513 0.44 -1.7 9,317 - GFF6513 0.44 -3.1 9,658 + GFF6513 0.63 -1.8 9,658 + GFF6513 0.63 -2.0 9,658 + GFF6513 0.63 -1.8 9,658 + GFF6513 0.63 -0.4 9,658 + GFF6513 0.63 -1.2 9,659 - GFF6513 0.63 -2.8 9,659 - GFF6513 0.63 -0.8 9,659 - GFF6513 0.63 -2.3 9,685 + GFF6513 0.64 -3.0 9,686 - GFF6513 0.64 -4.2 10,079 - GFF6513 0.86 -0.8 10,108 + GFF6513 0.87 -2.2 10,109 - GFF6513 0.87 -3.4 10,261 + -0.4 10,262 - -1.6 10,399 + +0.2 10,399 + -0.4 10,400 - +0.3 10,400 - -2.3 10,446 - +0.2 10,454 - +0.7 10,454 - -0.2 10,466 + +0.0 10,616 + -0.4 10,617 - +0.4 10,617 - +0.2 10,663 + +0.0 10,663 + -0.6 10,664 - -0.3 10,664 - +1.0 10,664 - -0.8 11,086 - GFF6514 0.53 +0.5 11,134 - GFF6514 0.59 -0.5 11,134 - GFF6514 0.59 -0.0 11,134 - GFF6514 0.59 +0.6 11,140 + GFF6514 0.60 -0.8 11,140 + GFF6514 0.60 -0.3 11,141 - GFF6514 0.60 +0.2 11,244 + GFF6514 0.72 -0.0 11,244 + GFF6514 0.72 +0.1 11,332 - GFF6514 0.83 +1.6 11,332 - GFF6514 0.83 +0.1 11,332 - GFF6514 0.83 +0.2
Or see this region's nucleotide sequence