Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6499 and GFF6500 are separated by 122 nucleotides GFF6500 and GFF6501 are separated by 52 nucleotides GFF6501 and GFF6502 are separated by 32 nucleotides
GFF6499 - hypothetical protein, at 76,369 to 76,848
GFF6499
GFF6500 - 2-hydroxychromene-2-carboxylate isomerase/DsbA-like thioredoxin domain, at 76,971 to 77,657
GFF6500
GFF6501 - Uncharacterized MFS-type transporter, at 77,710 to 79,119
GFF6501
GFF6502 - 4-hydroxyphenylpyruvate dioxygenase (EC 1.13.11.27), at 79,152 to 80,150
GFF6502
Position (kb)
77
78
79
80 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 76.748 kb on + strand, within GFF6499 at 76.749 kb on - strand, within GFF6499 at 76.767 kb on - strand, within GFF6499 at 76.832 kb on - strand at 76.956 kb on + strand at 76.967 kb on + strand at 76.967 kb on + strand at 76.967 kb on + strand at 76.967 kb on + strand at 76.968 kb on - strand at 77.113 kb on + strand, within GFF6500 at 77.113 kb on + strand, within GFF6500 at 77.113 kb on + strand, within GFF6500 at 77.113 kb on + strand, within GFF6500 at 77.114 kb on - strand, within GFF6500 at 77.114 kb on - strand, within GFF6500 at 77.114 kb on - strand, within GFF6500 at 77.114 kb on - strand, within GFF6500 at 77.114 kb on - strand, within GFF6500 at 77.114 kb on - strand, within GFF6500 at 77.114 kb on - strand, within GFF6500 at 77.114 kb on - strand, within GFF6500 at 77.254 kb on + strand, within GFF6500 at 77.254 kb on + strand, within GFF6500 at 77.255 kb on - strand, within GFF6500 at 77.347 kb on + strand, within GFF6500 at 77.347 kb on + strand, within GFF6500 at 77.347 kb on + strand, within GFF6500 at 77.347 kb on + strand, within GFF6500 at 77.450 kb on - strand, within GFF6500 at 77.450 kb on - strand, within GFF6500 at 77.768 kb on + strand at 77.769 kb on - strand at 77.769 kb on - strand at 77.769 kb on - strand at 78.056 kb on + strand, within GFF6501 at 78.066 kb on - strand, within GFF6501 at 78.131 kb on + strand, within GFF6501 at 78.131 kb on + strand, within GFF6501 at 78.132 kb on - strand, within GFF6501 at 78.132 kb on - strand, within GFF6501 at 78.137 kb on + strand, within GFF6501 at 78.137 kb on + strand, within GFF6501 at 78.138 kb on - strand, within GFF6501 at 78.138 kb on - strand, within GFF6501 at 78.138 kb on - strand, within GFF6501 at 78.272 kb on + strand, within GFF6501 at 78.272 kb on + strand, within GFF6501 at 78.272 kb on + strand, within GFF6501 at 78.273 kb on - strand, within GFF6501 at 78.273 kb on - strand, within GFF6501 at 78.273 kb on - strand, within GFF6501 at 78.273 kb on - strand, within GFF6501 at 78.273 kb on - strand, within GFF6501 at 78.273 kb on - strand, within GFF6501 at 78.273 kb on - strand, within GFF6501 at 78.273 kb on - strand, within GFF6501 at 78.317 kb on + strand, within GFF6501 at 78.318 kb on - strand, within GFF6501 at 78.318 kb on - strand, within GFF6501 at 78.497 kb on + strand, within GFF6501 at 78.497 kb on + strand, within GFF6501 at 78.498 kb on - strand, within GFF6501 at 78.498 kb on - strand, within GFF6501 at 78.498 kb on - strand, within GFF6501 at 78.498 kb on - strand, within GFF6501 at 78.575 kb on + strand, within GFF6501 at 78.575 kb on + strand, within GFF6501 at 78.575 kb on + strand, within GFF6501 at 78.575 kb on + strand, within GFF6501 at 78.575 kb on + strand, within GFF6501 at 78.575 kb on + strand, within GFF6501 at 78.575 kb on + strand, within GFF6501 at 78.575 kb on + strand, within GFF6501 at 78.575 kb on + strand, within GFF6501 at 78.575 kb on + strand, within GFF6501 at 78.575 kb on + strand, within GFF6501 at 78.575 kb on + strand, within GFF6501 at 78.575 kb on + strand, within GFF6501 at 78.576 kb on - strand, within GFF6501 at 78.576 kb on - strand, within GFF6501 at 78.576 kb on - strand, within GFF6501 at 78.576 kb on - strand, within GFF6501 at 78.576 kb on - strand, within GFF6501 at 78.576 kb on - strand, within GFF6501 at 78.576 kb on - strand, within GFF6501 at 78.785 kb on + strand, within GFF6501 at 78.785 kb on + strand, within GFF6501 at 78.785 kb on + strand, within GFF6501 at 78.786 kb on - strand, within GFF6501 at 78.786 kb on - strand, within GFF6501 at 78.786 kb on - strand, within GFF6501 at 78.786 kb on - strand, within GFF6501 at 78.786 kb on - strand, within GFF6501 at 78.915 kb on - strand, within GFF6501 at 78.926 kb on + strand, within GFF6501 at 78.927 kb on - strand, within GFF6501 at 79.013 kb on + strand at 79.013 kb on + strand at 79.013 kb on + strand at 79.029 kb on - strand at 79.029 kb on - strand at 79.029 kb on - strand at 79.201 kb on + strand at 79.201 kb on + strand at 79.202 kb on - strand at 79.202 kb on - strand at 79.202 kb on - strand at 79.210 kb on + strand at 79.210 kb on + strand at 79.267 kb on + strand, within GFF6502 at 79.267 kb on + strand, within GFF6502 at 79.267 kb on + strand, within GFF6502 at 79.267 kb on + strand, within GFF6502 at 79.268 kb on - strand, within GFF6502 at 79.268 kb on - strand, within GFF6502 at 79.271 kb on + strand, within GFF6502 at 79.271 kb on + strand, within GFF6502 at 79.272 kb on - strand, within GFF6502 at 79.272 kb on - strand, within GFF6502 at 79.280 kb on - strand, within GFF6502 at 79.378 kb on + strand, within GFF6502 at 79.378 kb on + strand, within GFF6502 at 79.378 kb on + strand, within GFF6502 at 79.378 kb on + strand, within GFF6502 at 79.379 kb on - strand, within GFF6502 at 79.379 kb on - strand, within GFF6502 at 79.665 kb on - strand, within GFF6502 at 79.768 kb on + strand, within GFF6502 at 79.768 kb on + strand, within GFF6502 at 79.769 kb on - strand, within GFF6502 at 79.769 kb on - strand, within GFF6502 at 79.769 kb on - strand, within GFF6502 at 79.769 kb on - strand, within GFF6502 at 80.032 kb on + strand, within GFF6502 at 80.033 kb on - strand, within GFF6502 at 80.073 kb on - strand at 80.073 kb on - strand at 80.089 kb on - strand at 80.089 kb on - strand at 80.089 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 76,748 + GFF6499 0.79 -0.5 76,749 - GFF6499 0.79 +1.4 76,767 - GFF6499 0.83 -0.3 76,832 - -0.8 76,956 + -2.3 76,967 + -1.3 76,967 + -0.4 76,967 + +0.1 76,967 + +0.0 76,968 - +0.1 77,113 + GFF6500 0.21 -1.2 77,113 + GFF6500 0.21 +0.3 77,113 + GFF6500 0.21 -0.1 77,113 + GFF6500 0.21 -0.6 77,114 - GFF6500 0.21 -0.0 77,114 - GFF6500 0.21 -0.1 77,114 - GFF6500 0.21 -1.0 77,114 - GFF6500 0.21 -1.3 77,114 - GFF6500 0.21 -0.2 77,114 - GFF6500 0.21 -1.4 77,114 - GFF6500 0.21 -0.1 77,114 - GFF6500 0.21 +0.0 77,254 + GFF6500 0.41 +0.4 77,254 + GFF6500 0.41 -0.1 77,255 - GFF6500 0.41 -0.2 77,347 + GFF6500 0.55 -0.9 77,347 + GFF6500 0.55 -0.3 77,347 + GFF6500 0.55 -0.6 77,347 + GFF6500 0.55 -0.3 77,450 - GFF6500 0.70 -1.9 77,450 - GFF6500 0.70 -0.1 77,768 + -0.6 77,769 - +0.6 77,769 - -0.2 77,769 - +0.6 78,056 + GFF6501 0.25 -2.0 78,066 - GFF6501 0.25 -0.7 78,131 + GFF6501 0.30 -0.4 78,131 + GFF6501 0.30 +0.5 78,132 - GFF6501 0.30 -1.0 78,132 - GFF6501 0.30 -0.6 78,137 + GFF6501 0.30 -1.7 78,137 + GFF6501 0.30 +0.8 78,138 - GFF6501 0.30 -0.0 78,138 - GFF6501 0.30 +0.6 78,138 - GFF6501 0.30 -0.1 78,272 + GFF6501 0.40 +0.9 78,272 + GFF6501 0.40 -0.8 78,272 + GFF6501 0.40 -0.0 78,273 - GFF6501 0.40 +0.4 78,273 - GFF6501 0.40 +0.1 78,273 - GFF6501 0.40 -0.9 78,273 - GFF6501 0.40 -1.1 78,273 - GFF6501 0.40 +0.8 78,273 - GFF6501 0.40 -1.2 78,273 - GFF6501 0.40 +0.6 78,273 - GFF6501 0.40 -0.3 78,317 + GFF6501 0.43 -2.1 78,318 - GFF6501 0.43 -0.2 78,318 - GFF6501 0.43 +0.3 78,497 + GFF6501 0.56 -0.4 78,497 + GFF6501 0.56 -1.7 78,498 - GFF6501 0.56 -0.6 78,498 - GFF6501 0.56 +0.0 78,498 - GFF6501 0.56 -0.7 78,498 - GFF6501 0.56 -0.4 78,575 + GFF6501 0.61 +0.9 78,575 + GFF6501 0.61 -1.0 78,575 + GFF6501 0.61 -1.4 78,575 + GFF6501 0.61 -0.8 78,575 + GFF6501 0.61 -1.2 78,575 + GFF6501 0.61 +0.2 78,575 + GFF6501 0.61 -0.3 78,575 + GFF6501 0.61 -1.7 78,575 + GFF6501 0.61 +0.3 78,575 + GFF6501 0.61 +0.6 78,575 + GFF6501 0.61 -0.3 78,575 + GFF6501 0.61 +1.6 78,575 + GFF6501 0.61 -0.4 78,576 - GFF6501 0.61 -0.4 78,576 - GFF6501 0.61 -1.3 78,576 - GFF6501 0.61 -1.5 78,576 - GFF6501 0.61 -0.0 78,576 - GFF6501 0.61 -0.4 78,576 - GFF6501 0.61 -1.4 78,576 - GFF6501 0.61 -0.6 78,785 + GFF6501 0.76 +1.0 78,785 + GFF6501 0.76 +0.8 78,785 + GFF6501 0.76 -1.0 78,786 - GFF6501 0.76 -2.6 78,786 - GFF6501 0.76 -0.3 78,786 - GFF6501 0.76 -0.5 78,786 - GFF6501 0.76 +0.4 78,786 - GFF6501 0.76 -1.1 78,915 - GFF6501 0.85 +0.2 78,926 + GFF6501 0.86 -0.7 78,927 - GFF6501 0.86 -0.7 79,013 + +0.6 79,013 + -0.2 79,013 + -1.0 79,029 - +0.5 79,029 - -0.9 79,029 - -1.9 79,201 + -2.7 79,201 + -0.6 79,202 - +0.1 79,202 - +0.4 79,202 - +0.1 79,210 + +0.3 79,210 + -0.4 79,267 + GFF6502 0.12 -0.1 79,267 + GFF6502 0.12 +0.3 79,267 + GFF6502 0.12 +0.0 79,267 + GFF6502 0.12 +0.1 79,268 - GFF6502 0.12 -0.6 79,268 - GFF6502 0.12 +0.3 79,271 + GFF6502 0.12 -0.2 79,271 + GFF6502 0.12 +0.5 79,272 - GFF6502 0.12 -0.4 79,272 - GFF6502 0.12 -1.5 79,280 - GFF6502 0.13 +0.2 79,378 + GFF6502 0.23 -0.1 79,378 + GFF6502 0.23 +0.4 79,378 + GFF6502 0.23 +0.4 79,378 + GFF6502 0.23 -1.1 79,379 - GFF6502 0.23 -0.4 79,379 - GFF6502 0.23 -1.2 79,665 - GFF6502 0.51 +1.0 79,768 + GFF6502 0.62 -0.3 79,768 + GFF6502 0.62 -1.0 79,769 - GFF6502 0.62 +0.9 79,769 - GFF6502 0.62 +0.0 79,769 - GFF6502 0.62 -0.7 79,769 - GFF6502 0.62 -1.5 80,032 + GFF6502 0.88 +0.3 80,033 - GFF6502 0.88 +0.2 80,073 - +0.3 80,073 - -0.6 80,089 - -0.0 80,089 - -0.6 80,089 - +2.1
Or see this region's nucleotide sequence