Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6399 and GFF6400 are separated by 40 nucleotides GFF6400 and GFF6401 are separated by 11 nucleotides GFF6401 and GFF6402 overlap by 4 nucleotides
GFF6399 - 5-methyltetrahydrofolate--homocysteine methyltransferase (EC 2.1.1.13), N-terminal domain, at 73,425 to 74,477
GFF6399
GFF6400 - N-formylglutamate deformylase (EC 3.5.1.68), at 74,518 to 75,342
GFF6400
GFF6401 - Formiminoglutamic iminohydrolase (EC 3.5.3.13), at 75,354 to 76,736
GFF6401
GFF6402 - Imidazolonepropionase (EC 3.5.2.7), at 76,733 to 77,959
GFF6402
Position (kb)
75
76
77 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 74.356 kb on + strand, within GFF6399 at 74.459 kb on + strand at 74.460 kb on - strand at 74.461 kb on + strand at 74.461 kb on + strand at 74.461 kb on + strand at 74.461 kb on + strand at 74.461 kb on + strand at 74.461 kb on + strand at 74.461 kb on + strand at 74.461 kb on + strand at 74.461 kb on + strand at 74.461 kb on + strand at 74.461 kb on + strand at 74.461 kb on + strand at 74.461 kb on + strand at 74.461 kb on + strand at 74.461 kb on + strand at 74.461 kb on + strand at 74.462 kb on - strand at 74.462 kb on - strand at 74.462 kb on - strand at 74.462 kb on - strand at 74.462 kb on - strand at 74.462 kb on - strand at 74.462 kb on - strand at 74.462 kb on - strand at 74.462 kb on - strand at 74.462 kb on - strand at 74.462 kb on - strand at 74.462 kb on - strand at 74.462 kb on - strand at 74.462 kb on - strand at 74.462 kb on - strand at 74.462 kb on - strand at 74.462 kb on - strand at 74.462 kb on - strand at 74.462 kb on - strand at 74.477 kb on + strand at 74.477 kb on + strand at 74.478 kb on - strand at 74.478 kb on - strand at 74.490 kb on + strand at 74.490 kb on + strand at 74.636 kb on + strand, within GFF6400 at 74.636 kb on + strand, within GFF6400 at 74.636 kb on + strand, within GFF6400 at 74.636 kb on + strand, within GFF6400 at 74.636 kb on + strand, within GFF6400 at 74.636 kb on + strand, within GFF6400 at 74.636 kb on + strand, within GFF6400 at 74.636 kb on + strand, within GFF6400 at 74.636 kb on + strand, within GFF6400 at 74.636 kb on + strand, within GFF6400 at 74.636 kb on + strand, within GFF6400 at 74.637 kb on - strand, within GFF6400 at 74.637 kb on - strand, within GFF6400 at 74.637 kb on - strand, within GFF6400 at 74.637 kb on - strand, within GFF6400 at 74.637 kb on - strand, within GFF6400 at 74.637 kb on - strand, within GFF6400 at 74.637 kb on - strand, within GFF6400 at 74.637 kb on - strand, within GFF6400 at 74.637 kb on - strand, within GFF6400 at 74.637 kb on - strand, within GFF6400 at 74.637 kb on - strand, within GFF6400 at 74.637 kb on - strand, within GFF6400 at 74.694 kb on - strand, within GFF6400 at 74.694 kb on - strand, within GFF6400 at 74.708 kb on + strand, within GFF6400 at 74.708 kb on + strand, within GFF6400 at 74.708 kb on + strand, within GFF6400 at 74.709 kb on - strand, within GFF6400 at 74.709 kb on - strand, within GFF6400 at 74.709 kb on - strand, within GFF6400 at 74.709 kb on - strand, within GFF6400 at 74.744 kb on + strand, within GFF6400 at 74.744 kb on + strand, within GFF6400 at 74.951 kb on + strand, within GFF6400 at 74.951 kb on + strand, within GFF6400 at 74.952 kb on - strand, within GFF6400 at 74.952 kb on - strand, within GFF6400 at 74.952 kb on - strand, within GFF6400 at 74.952 kb on - strand, within GFF6400 at 75.020 kb on + strand, within GFF6400 at 75.020 kb on + strand, within GFF6400 at 75.020 kb on + strand, within GFF6400 at 75.021 kb on - strand, within GFF6400 at 75.021 kb on - strand, within GFF6400 at 75.081 kb on - strand, within GFF6400 at 75.081 kb on - strand, within GFF6400 at 75.081 kb on - strand, within GFF6400 at 75.120 kb on + strand, within GFF6400 at 75.122 kb on + strand, within GFF6400 at 75.122 kb on + strand, within GFF6400 at 75.122 kb on + strand, within GFF6400 at 75.123 kb on - strand, within GFF6400 at 75.123 kb on - strand, within GFF6400 at 75.123 kb on - strand, within GFF6400 at 75.123 kb on - strand, within GFF6400 at 75.176 kb on + strand, within GFF6400 at 75.176 kb on + strand, within GFF6400 at 75.177 kb on - strand, within GFF6400 at 75.177 kb on - strand, within GFF6400 at 75.177 kb on - strand, within GFF6400 at 75.509 kb on + strand, within GFF6401 at 75.509 kb on + strand, within GFF6401 at 75.510 kb on - strand, within GFF6401 at 75.510 kb on - strand, within GFF6401 at 75.510 kb on - strand, within GFF6401 at 75.716 kb on - strand, within GFF6401 at 75.716 kb on - strand, within GFF6401 at 75.716 kb on - strand, within GFF6401 at 75.883 kb on + strand, within GFF6401 at 75.883 kb on + strand, within GFF6401 at 75.884 kb on - strand, within GFF6401 at 75.884 kb on - strand, within GFF6401 at 75.890 kb on - strand, within GFF6401 at 76.255 kb on + strand, within GFF6401 at 76.255 kb on + strand, within GFF6401 at 76.255 kb on + strand, within GFF6401 at 76.339 kb on + strand, within GFF6401 at 76.369 kb on + strand, within GFF6401 at 76.369 kb on + strand, within GFF6401 at 76.369 kb on + strand, within GFF6401 at 76.369 kb on + strand, within GFF6401 at 76.369 kb on + strand, within GFF6401 at 76.370 kb on - strand, within GFF6401 at 76.370 kb on - strand, within GFF6401 at 76.370 kb on - strand, within GFF6401 at 76.370 kb on - strand, within GFF6401 at 76.417 kb on + strand, within GFF6401 at 76.418 kb on - strand, within GFF6401 at 76.474 kb on + strand, within GFF6401 at 76.474 kb on + strand, within GFF6401 at 76.475 kb on - strand, within GFF6401 at 76.475 kb on - strand, within GFF6401 at 76.475 kb on - strand, within GFF6401 at 76.475 kb on - strand, within GFF6401 at 76.475 kb on - strand, within GFF6401 at 76.475 kb on - strand, within GFF6401 at 76.804 kb on - strand at 77.091 kb on + strand, within GFF6402 at 77.091 kb on + strand, within GFF6402 at 77.091 kb on + strand, within GFF6402 at 77.091 kb on + strand, within GFF6402 at 77.091 kb on + strand, within GFF6402 at 77.091 kb on + strand, within GFF6402 at 77.091 kb on + strand, within GFF6402 at 77.092 kb on - strand, within GFF6402 at 77.092 kb on - strand, within GFF6402 at 77.092 kb on - strand, within GFF6402 at 77.092 kb on - strand, within GFF6402 at 77.092 kb on - strand, within GFF6402 at 77.092 kb on - strand, within GFF6402 at 77.092 kb on - strand, within GFF6402 at 77.092 kb on - strand, within GFF6402 at 77.092 kb on - strand, within GFF6402 at 77.094 kb on + strand, within GFF6402 at 77.095 kb on - strand, within GFF6402 at 77.095 kb on - strand, within GFF6402 at 77.095 kb on - strand, within GFF6402 at 77.194 kb on - strand, within GFF6402 at 77.397 kb on + strand, within GFF6402 at 77.397 kb on + strand, within GFF6402 at 77.397 kb on + strand, within GFF6402 at 77.398 kb on - strand, within GFF6402 at 77.398 kb on - strand, within GFF6402 at 77.398 kb on - strand, within GFF6402 at 77.398 kb on - strand, within GFF6402 at 77.398 kb on - strand, within GFF6402 at 77.398 kb on - strand, within GFF6402 at 77.398 kb on - strand, within GFF6402 at 77.398 kb on - strand, within GFF6402 at 77.472 kb on + strand, within GFF6402 at 77.561 kb on + strand, within GFF6402 at 77.561 kb on + strand, within GFF6402 at 77.562 kb on - strand, within GFF6402 at 77.562 kb on - strand, within GFF6402 at 77.562 kb on - strand, within GFF6402 at 77.562 kb on - strand, within GFF6402 at 77.562 kb on - strand, within GFF6402 at 77.562 kb on - strand, within GFF6402 at 77.562 kb on - strand, within GFF6402 at 77.562 kb on - strand, within GFF6402 at 77.685 kb on + strand, within GFF6402 at 77.685 kb on + strand, within GFF6402 at 77.685 kb on + strand, within GFF6402 at 77.686 kb on - strand, within GFF6402 at 77.736 kb on + strand, within GFF6402
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 74,356 + GFF6399 0.88 -0.1 74,459 + -0.5 74,460 - +0.7 74,461 + +0.1 74,461 + +0.2 74,461 + -0.4 74,461 + -0.3 74,461 + +1.1 74,461 + +0.2 74,461 + +0.1 74,461 + +0.1 74,461 + -2.5 74,461 + +0.3 74,461 + -2.1 74,461 + +0.3 74,461 + -1.3 74,461 + +0.0 74,461 + -0.2 74,461 + -0.8 74,462 - -1.8 74,462 - -1.4 74,462 - -0.0 74,462 - -0.3 74,462 - -0.5 74,462 - -0.0 74,462 - -0.6 74,462 - -0.5 74,462 - +0.8 74,462 - -1.1 74,462 - -0.8 74,462 - +0.4 74,462 - -0.3 74,462 - +0.8 74,462 - -0.2 74,462 - +0.0 74,462 - -3.2 74,462 - -0.4 74,462 - +0.1 74,477 + -0.3 74,477 + -0.1 74,478 - +0.7 74,478 - -0.4 74,490 + +1.3 74,490 + -0.4 74,636 + GFF6400 0.14 +1.9 74,636 + GFF6400 0.14 -2.1 74,636 + GFF6400 0.14 -0.2 74,636 + GFF6400 0.14 -1.0 74,636 + GFF6400 0.14 +0.3 74,636 + GFF6400 0.14 +0.2 74,636 + GFF6400 0.14 -0.9 74,636 + GFF6400 0.14 +0.6 74,636 + GFF6400 0.14 -1.4 74,636 + GFF6400 0.14 -0.7 74,636 + GFF6400 0.14 -0.8 74,637 - GFF6400 0.14 -0.8 74,637 - GFF6400 0.14 -0.0 74,637 - GFF6400 0.14 +2.9 74,637 - GFF6400 0.14 -1.4 74,637 - GFF6400 0.14 +0.0 74,637 - GFF6400 0.14 +1.6 74,637 - GFF6400 0.14 +0.9 74,637 - GFF6400 0.14 +0.7 74,637 - GFF6400 0.14 -0.2 74,637 - GFF6400 0.14 +1.7 74,637 - GFF6400 0.14 +0.0 74,637 - GFF6400 0.14 -1.6 74,694 - GFF6400 0.21 -0.2 74,694 - GFF6400 0.21 +0.2 74,708 + GFF6400 0.23 -0.9 74,708 + GFF6400 0.23 -0.4 74,708 + GFF6400 0.23 -1.1 74,709 - GFF6400 0.23 -0.8 74,709 - GFF6400 0.23 +0.5 74,709 - GFF6400 0.23 -0.1 74,709 - GFF6400 0.23 -0.9 74,744 + GFF6400 0.27 -0.4 74,744 + GFF6400 0.27 +0.7 74,951 + GFF6400 0.52 +0.5 74,951 + GFF6400 0.52 -0.3 74,952 - GFF6400 0.53 +0.4 74,952 - GFF6400 0.53 -0.3 74,952 - GFF6400 0.53 +0.1 74,952 - GFF6400 0.53 -1.4 75,020 + GFF6400 0.61 -0.4 75,020 + GFF6400 0.61 -1.4 75,020 + GFF6400 0.61 -0.4 75,021 - GFF6400 0.61 +0.2 75,021 - GFF6400 0.61 +0.2 75,081 - GFF6400 0.68 +0.3 75,081 - GFF6400 0.68 -1.3 75,081 - GFF6400 0.68 -0.9 75,120 + GFF6400 0.73 -0.6 75,122 + GFF6400 0.73 -0.1 75,122 + GFF6400 0.73 +0.1 75,122 + GFF6400 0.73 -0.6 75,123 - GFF6400 0.73 -0.4 75,123 - GFF6400 0.73 -0.5 75,123 - GFF6400 0.73 -0.3 75,123 - GFF6400 0.73 -1.1 75,176 + GFF6400 0.80 +0.4 75,176 + GFF6400 0.80 -0.6 75,177 - GFF6400 0.80 -0.7 75,177 - GFF6400 0.80 -0.4 75,177 - GFF6400 0.80 +0.3 75,509 + GFF6401 0.11 -0.7 75,509 + GFF6401 0.11 +1.1 75,510 - GFF6401 0.11 -0.3 75,510 - GFF6401 0.11 -0.4 75,510 - GFF6401 0.11 -0.9 75,716 - GFF6401 0.26 -0.9 75,716 - GFF6401 0.26 -0.1 75,716 - GFF6401 0.26 -0.2 75,883 + GFF6401 0.38 +0.1 75,883 + GFF6401 0.38 -1.3 75,884 - GFF6401 0.38 +1.3 75,884 - GFF6401 0.38 -0.0 75,890 - GFF6401 0.39 +0.4 76,255 + GFF6401 0.65 +0.5 76,255 + GFF6401 0.65 -1.4 76,255 + GFF6401 0.65 +1.1 76,339 + GFF6401 0.71 -1.8 76,369 + GFF6401 0.73 -0.9 76,369 + GFF6401 0.73 -0.9 76,369 + GFF6401 0.73 +0.5 76,369 + GFF6401 0.73 -0.1 76,369 + GFF6401 0.73 -0.1 76,370 - GFF6401 0.73 -1.4 76,370 - GFF6401 0.73 +0.7 76,370 - GFF6401 0.73 -1.8 76,370 - GFF6401 0.73 +0.4 76,417 + GFF6401 0.77 -0.4 76,418 - GFF6401 0.77 +0.0 76,474 + GFF6401 0.81 -1.5 76,474 + GFF6401 0.81 +0.5 76,475 - GFF6401 0.81 -0.3 76,475 - GFF6401 0.81 +1.2 76,475 - GFF6401 0.81 -0.4 76,475 - GFF6401 0.81 +0.9 76,475 - GFF6401 0.81 -1.6 76,475 - GFF6401 0.81 -0.8 76,804 - -1.3 77,091 + GFF6402 0.29 -0.4 77,091 + GFF6402 0.29 -0.6 77,091 + GFF6402 0.29 -0.3 77,091 + GFF6402 0.29 -0.3 77,091 + GFF6402 0.29 -1.6 77,091 + GFF6402 0.29 +0.1 77,091 + GFF6402 0.29 -0.8 77,092 - GFF6402 0.29 -1.3 77,092 - GFF6402 0.29 -0.2 77,092 - GFF6402 0.29 -0.2 77,092 - GFF6402 0.29 +0.7 77,092 - GFF6402 0.29 +1.6 77,092 - GFF6402 0.29 -0.4 77,092 - GFF6402 0.29 +0.1 77,092 - GFF6402 0.29 -0.1 77,092 - GFF6402 0.29 -1.1 77,094 + GFF6402 0.29 +0.3 77,095 - GFF6402 0.30 -0.3 77,095 - GFF6402 0.30 -1.4 77,095 - GFF6402 0.30 -0.7 77,194 - GFF6402 0.38 +0.6 77,397 + GFF6402 0.54 +0.9 77,397 + GFF6402 0.54 -1.0 77,397 + GFF6402 0.54 -0.4 77,398 - GFF6402 0.54 -0.1 77,398 - GFF6402 0.54 +1.3 77,398 - GFF6402 0.54 -0.2 77,398 - GFF6402 0.54 -0.1 77,398 - GFF6402 0.54 -0.7 77,398 - GFF6402 0.54 +0.2 77,398 - GFF6402 0.54 -2.1 77,398 - GFF6402 0.54 -1.3 77,472 + GFF6402 0.60 -0.2 77,561 + GFF6402 0.67 -0.7 77,561 + GFF6402 0.67 +0.1 77,562 - GFF6402 0.68 +0.1 77,562 - GFF6402 0.68 +0.2 77,562 - GFF6402 0.68 +1.6 77,562 - GFF6402 0.68 -0.1 77,562 - GFF6402 0.68 -0.4 77,562 - GFF6402 0.68 +0.1 77,562 - GFF6402 0.68 -1.3 77,562 - GFF6402 0.68 -0.0 77,685 + GFF6402 0.78 +0.7 77,685 + GFF6402 0.78 +0.7 77,685 + GFF6402 0.78 +1.9 77,686 - GFF6402 0.78 -1.9 77,736 + GFF6402 0.82 -0.2
Or see this region's nucleotide sequence