Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6389 and GFF6390 are separated by 9 nucleotides GFF6390 and GFF6391 overlap by 4 nucleotides GFF6391 and GFF6392 overlap by 4 nucleotides
GFF6389 - Amidohydrolase domain protein, at 61,667 to 62,866
GFF6389
GFF6390 - GtrA-like protein, at 62,876 to 63,277
GFF6390
GFF6391 - RfbJ protein, at 63,274 to 64,242
GFF6391
GFF6392 - hypothetical protein, at 64,239 to 66,074
GFF6392
Position (kb)
62
63
64 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 62.213 kb on + strand, within GFF6389 at 62.249 kb on + strand, within GFF6389 at 62.250 kb on - strand, within GFF6389 at 62.368 kb on + strand, within GFF6389 at 62.369 kb on - strand, within GFF6389 at 62.369 kb on - strand, within GFF6389 at 62.369 kb on - strand, within GFF6389 at 62.908 kb on - strand at 62.908 kb on - strand at 63.009 kb on + strand, within GFF6390 at 63.009 kb on + strand, within GFF6390 at 63.009 kb on + strand, within GFF6390 at 63.009 kb on + strand, within GFF6390 at 63.009 kb on + strand, within GFF6390 at 63.009 kb on + strand, within GFF6390 at 63.009 kb on + strand, within GFF6390 at 63.009 kb on + strand, within GFF6390 at 63.010 kb on - strand, within GFF6390 at 63.010 kb on - strand, within GFF6390 at 63.010 kb on - strand, within GFF6390 at 63.010 kb on - strand, within GFF6390 at 63.018 kb on + strand, within GFF6390 at 63.018 kb on + strand, within GFF6390 at 63.019 kb on - strand, within GFF6390 at 63.019 kb on - strand, within GFF6390 at 63.055 kb on - strand, within GFF6390 at 63.055 kb on - strand, within GFF6390 at 63.063 kb on + strand, within GFF6390 at 63.063 kb on + strand, within GFF6390 at 63.064 kb on - strand, within GFF6390 at 63.064 kb on - strand, within GFF6390 at 63.111 kb on + strand, within GFF6390 at 63.111 kb on + strand, within GFF6390 at 63.111 kb on + strand, within GFF6390 at 63.112 kb on - strand, within GFF6390 at 63.112 kb on - strand, within GFF6390 at 63.112 kb on - strand, within GFF6390 at 63.175 kb on - strand, within GFF6390 at 63.202 kb on - strand, within GFF6390 at 63.202 kb on - strand, within GFF6390 at 63.202 kb on - strand, within GFF6390 at 63.314 kb on + strand at 63.314 kb on + strand at 63.314 kb on + strand at 63.315 kb on - strand at 63.315 kb on - strand at 63.315 kb on - strand at 63.455 kb on + strand, within GFF6391 at 63.455 kb on + strand, within GFF6391 at 63.455 kb on + strand, within GFF6391 at 63.455 kb on + strand, within GFF6391 at 63.455 kb on + strand, within GFF6391 at 63.455 kb on + strand, within GFF6391 at 63.455 kb on + strand, within GFF6391 at 63.456 kb on - strand, within GFF6391 at 63.456 kb on - strand, within GFF6391 at 63.456 kb on - strand, within GFF6391 at 63.456 kb on - strand, within GFF6391 at 63.456 kb on - strand, within GFF6391 at 63.456 kb on - strand, within GFF6391 at 63.456 kb on - strand, within GFF6391 at 63.456 kb on - strand, within GFF6391 at 63.456 kb on - strand, within GFF6391 at 63.456 kb on - strand, within GFF6391 at 63.514 kb on + strand, within GFF6391 at 63.591 kb on - strand, within GFF6391 at 63.632 kb on + strand, within GFF6391 at 63.632 kb on + strand, within GFF6391 at 63.633 kb on - strand, within GFF6391 at 63.633 kb on - strand, within GFF6391 at 63.633 kb on - strand, within GFF6391 at 63.633 kb on - strand, within GFF6391 at 63.633 kb on - strand, within GFF6391 at 63.653 kb on + strand, within GFF6391 at 63.653 kb on + strand, within GFF6391 at 63.654 kb on - strand, within GFF6391 at 63.848 kb on + strand, within GFF6391 at 63.950 kb on + strand, within GFF6391 at 63.951 kb on - strand, within GFF6391 at 63.951 kb on - strand, within GFF6391 at 63.977 kb on + strand, within GFF6391 at 63.977 kb on + strand, within GFF6391 at 63.978 kb on - strand, within GFF6391 at 63.978 kb on - strand, within GFF6391 at 64.181 kb on + strand at 64.181 kb on + strand at 64.181 kb on + strand at 64.181 kb on + strand at 64.181 kb on + strand at 64.182 kb on - strand at 64.182 kb on - strand at 64.182 kb on - strand at 64.182 kb on - strand at 64.182 kb on - strand at 64.182 kb on - strand at 64.182 kb on - strand at 64.182 kb on - strand at 64.182 kb on - strand at 64.260 kb on - strand at 64.264 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 62,213 + GFF6389 0.46 -1.5 62,249 + GFF6389 0.48 -1.0 62,250 - GFF6389 0.49 +0.2 62,368 + GFF6389 0.58 -0.2 62,369 - GFF6389 0.58 -0.7 62,369 - GFF6389 0.58 -0.0 62,369 - GFF6389 0.58 -0.7 62,908 - -1.6 62,908 - -0.6 63,009 + GFF6390 0.33 +0.7 63,009 + GFF6390 0.33 -1.4 63,009 + GFF6390 0.33 +0.6 63,009 + GFF6390 0.33 -0.6 63,009 + GFF6390 0.33 -1.0 63,009 + GFF6390 0.33 -0.4 63,009 + GFF6390 0.33 +1.1 63,009 + GFF6390 0.33 +0.2 63,010 - GFF6390 0.33 +0.3 63,010 - GFF6390 0.33 +0.9 63,010 - GFF6390 0.33 +1.9 63,010 - GFF6390 0.33 +0.6 63,018 + GFF6390 0.35 +0.1 63,018 + GFF6390 0.35 -1.4 63,019 - GFF6390 0.36 -0.6 63,019 - GFF6390 0.36 +0.1 63,055 - GFF6390 0.45 -0.3 63,055 - GFF6390 0.45 -0.3 63,063 + GFF6390 0.47 +0.4 63,063 + GFF6390 0.47 -0.4 63,064 - GFF6390 0.47 -0.3 63,064 - GFF6390 0.47 +0.3 63,111 + GFF6390 0.58 -1.8 63,111 + GFF6390 0.58 -0.9 63,111 + GFF6390 0.58 -1.2 63,112 - GFF6390 0.59 +0.1 63,112 - GFF6390 0.59 -0.1 63,112 - GFF6390 0.59 -1.4 63,175 - GFF6390 0.74 +1.5 63,202 - GFF6390 0.81 -1.3 63,202 - GFF6390 0.81 -0.9 63,202 - GFF6390 0.81 -0.9 63,314 + -1.6 63,314 + +0.6 63,314 + +1.1 63,315 - -1.1 63,315 - -0.2 63,315 - +0.2 63,455 + GFF6391 0.19 -1.4 63,455 + GFF6391 0.19 +1.1 63,455 + GFF6391 0.19 +0.2 63,455 + GFF6391 0.19 +0.7 63,455 + GFF6391 0.19 -2.1 63,455 + GFF6391 0.19 -0.5 63,455 + GFF6391 0.19 +0.2 63,456 - GFF6391 0.19 -0.1 63,456 - GFF6391 0.19 +0.9 63,456 - GFF6391 0.19 +0.4 63,456 - GFF6391 0.19 -0.4 63,456 - GFF6391 0.19 +0.7 63,456 - GFF6391 0.19 -0.4 63,456 - GFF6391 0.19 -1.3 63,456 - GFF6391 0.19 +0.0 63,456 - GFF6391 0.19 -1.4 63,456 - GFF6391 0.19 -0.5 63,514 + GFF6391 0.25 +0.7 63,591 - GFF6391 0.33 -0.4 63,632 + GFF6391 0.37 +1.0 63,632 + GFF6391 0.37 -0.5 63,633 - GFF6391 0.37 +1.1 63,633 - GFF6391 0.37 -0.2 63,633 - GFF6391 0.37 -0.2 63,633 - GFF6391 0.37 -0.3 63,633 - GFF6391 0.37 +0.2 63,653 + GFF6391 0.39 -0.9 63,653 + GFF6391 0.39 +0.1 63,654 - GFF6391 0.39 +0.1 63,848 + GFF6391 0.59 -0.4 63,950 + GFF6391 0.70 +1.1 63,951 - GFF6391 0.70 -0.1 63,951 - GFF6391 0.70 +0.3 63,977 + GFF6391 0.73 -0.3 63,977 + GFF6391 0.73 +1.1 63,978 - GFF6391 0.73 +0.0 63,978 - GFF6391 0.73 -0.7 64,181 + +0.7 64,181 + +1.3 64,181 + +0.2 64,181 + -0.0 64,181 + -1.1 64,182 - +0.9 64,182 - -1.7 64,182 - -1.9 64,182 - +0.0 64,182 - -0.6 64,182 - -0.3 64,182 - -0.9 64,182 - +0.4 64,182 - +1.1 64,260 - -0.1 64,264 + +1.1
Or see this region's nucleotide sequence