Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6365 and GFF6366 overlap by 4 nucleotides GFF6366 and GFF6367 overlap by 4 nucleotides
GFF6365 - T6SS AAA+ chaperone ClpV (TssH), at 32,389 to 35,109
GFF6365
GFF6366 - T6SS component TssG (ImpH/VasB), at 35,106 to 36,281
GFF6366
GFF6367 - T6SS component TssF (ImpG/VasA), at 36,278 to 38,149
GFF6367
Position (kb) 35
36
37 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 34.155 kb on + strand, within GFF6365 at 34.529 kb on + strand, within GFF6365 at 34.529 kb on + strand, within GFF6365 at 34.529 kb on + strand, within GFF6365 at 34.763 kb on + strand, within GFF6365 at 34.763 kb on + strand, within GFF6365 at 35.006 kb on + strand at 35.006 kb on + strand at 35.057 kb on + strand at 35.058 kb on - strand at 35.058 kb on - strand at 35.058 kb on - strand at 35.315 kb on - strand, within GFF6366 at 35.580 kb on + strand, within GFF6366 at 35.580 kb on + strand, within GFF6366 at 35.581 kb on - strand, within GFF6366 at 35.581 kb on - strand, within GFF6366 at 35.704 kb on + strand, within GFF6366 at 35.705 kb on - strand, within GFF6366 at 35.705 kb on - strand, within GFF6366 at 35.705 kb on - strand, within GFF6366 at 35.774 kb on - strand, within GFF6366 at 36.194 kb on - strand at 36.194 kb on - strand at 36.417 kb on + strand at 36.586 kb on - strand, within GFF6367 at 36.651 kb on + strand, within GFF6367 at 36.651 kb on + strand, within GFF6367 at 36.742 kb on + strand, within GFF6367 at 36.743 kb on - strand, within GFF6367 at 36.897 kb on + strand, within GFF6367 at 36.897 kb on + strand, within GFF6367 at 36.897 kb on + strand, within GFF6367 at 36.897 kb on + strand, within GFF6367 at 36.916 kb on - strand, within GFF6367 at 36.916 kb on - strand, within GFF6367 at 36.985 kb on - strand, within GFF6367
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 34,155 + GFF6365 0.65 -0.9 34,529 + GFF6365 0.79 +0.9 34,529 + GFF6365 0.79 +1.0 34,529 + GFF6365 0.79 +1.3 34,763 + GFF6365 0.87 -0.2 34,763 + GFF6365 0.87 -0.3 35,006 + +0.6 35,006 + -0.5 35,057 + +0.5 35,058 - +0.3 35,058 - -0.3 35,058 - -0.6 35,315 - GFF6366 0.18 -1.6 35,580 + GFF6366 0.40 -0.2 35,580 + GFF6366 0.40 -2.0 35,581 - GFF6366 0.40 -1.3 35,581 - GFF6366 0.40 -0.4 35,704 + GFF6366 0.51 -0.1 35,705 - GFF6366 0.51 -0.4 35,705 - GFF6366 0.51 +2.9 35,705 - GFF6366 0.51 -0.7 35,774 - GFF6366 0.57 -0.9 36,194 - -0.4 36,194 - +0.4 36,417 + +0.2 36,586 - GFF6367 0.16 +0.3 36,651 + GFF6367 0.20 +0.2 36,651 + GFF6367 0.20 -0.0 36,742 + GFF6367 0.25 +0.5 36,743 - GFF6367 0.25 +0.3 36,897 + GFF6367 0.33 -1.7 36,897 + GFF6367 0.33 -0.4 36,897 + GFF6367 0.33 -0.2 36,897 + GFF6367 0.33 -0.4 36,916 - GFF6367 0.34 +1.0 36,916 - GFF6367 0.34 -2.2 36,985 - GFF6367 0.38 +0.5
Or see this region's nucleotide sequence