Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6331 and GFF6332 are separated by 97 nucleotides GFF6332 and GFF6333 are separated by 5 nucleotides
GFF6331 - D-octopine dehydrogenase (EC 1.5.1.11), at 125,578 to 126,648
GFF6331
GFF6332 - Maltodextrin glucosidase (EC 3.2.1.20), at 126,746 to 128,434
GFF6332
GFF6333 - 1,4-alpha-glucan (glycogen) branching enzyme, GH-13-type (EC 2.4.1.18), at 128,440 to 130,614
GFF6333
Position (kb)
126
127
128
129 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 125.807 kb on + strand, within GFF6331 at 125.807 kb on + strand, within GFF6331 at 125.807 kb on + strand, within GFF6331 at 125.807 kb on + strand, within GFF6331 at 125.807 kb on + strand, within GFF6331 at 125.807 kb on + strand, within GFF6331 at 125.807 kb on + strand, within GFF6331 at 125.807 kb on + strand, within GFF6331 at 125.808 kb on - strand, within GFF6331 at 125.808 kb on - strand, within GFF6331 at 125.808 kb on - strand, within GFF6331 at 125.808 kb on - strand, within GFF6331 at 125.808 kb on - strand, within GFF6331 at 125.808 kb on - strand, within GFF6331 at 125.808 kb on - strand, within GFF6331 at 125.920 kb on + strand, within GFF6331 at 125.920 kb on + strand, within GFF6331 at 125.920 kb on + strand, within GFF6331 at 125.920 kb on + strand, within GFF6331 at 125.920 kb on + strand, within GFF6331 at 125.921 kb on - strand, within GFF6331 at 125.921 kb on - strand, within GFF6331 at 125.921 kb on - strand, within GFF6331 at 125.921 kb on - strand, within GFF6331 at 125.921 kb on - strand, within GFF6331 at 126.069 kb on + strand, within GFF6331 at 126.070 kb on - strand, within GFF6331 at 126.235 kb on + strand, within GFF6331 at 126.236 kb on - strand, within GFF6331 at 126.318 kb on - strand, within GFF6331 at 126.318 kb on - strand, within GFF6331 at 126.448 kb on + strand, within GFF6331 at 126.448 kb on + strand, within GFF6331 at 126.448 kb on + strand, within GFF6331 at 126.449 kb on - strand, within GFF6331 at 126.449 kb on - strand, within GFF6331 at 126.449 kb on - strand, within GFF6331 at 126.449 kb on - strand, within GFF6331 at 126.469 kb on + strand, within GFF6331 at 126.469 kb on + strand, within GFF6331 at 126.692 kb on - strand at 126.803 kb on + strand at 126.803 kb on + strand at 126.803 kb on + strand at 126.803 kb on + strand at 126.804 kb on - strand at 126.812 kb on + strand at 126.812 kb on + strand at 126.812 kb on + strand at 126.813 kb on - strand at 126.813 kb on - strand at 127.145 kb on + strand, within GFF6332 at 127.145 kb on + strand, within GFF6332 at 127.145 kb on + strand, within GFF6332 at 127.145 kb on + strand, within GFF6332 at 127.145 kb on + strand, within GFF6332 at 127.145 kb on + strand, within GFF6332 at 127.145 kb on + strand, within GFF6332 at 127.145 kb on + strand, within GFF6332 at 127.145 kb on + strand, within GFF6332 at 127.146 kb on - strand, within GFF6332 at 127.146 kb on - strand, within GFF6332 at 127.146 kb on - strand, within GFF6332 at 127.146 kb on - strand, within GFF6332 at 127.244 kb on + strand, within GFF6332 at 127.244 kb on + strand, within GFF6332 at 127.244 kb on + strand, within GFF6332 at 127.244 kb on + strand, within GFF6332 at 127.244 kb on + strand, within GFF6332 at 127.244 kb on + strand, within GFF6332 at 127.245 kb on - strand, within GFF6332 at 127.245 kb on - strand, within GFF6332 at 127.245 kb on - strand, within GFF6332 at 127.247 kb on + strand, within GFF6332 at 127.247 kb on + strand, within GFF6332 at 127.248 kb on - strand, within GFF6332 at 127.248 kb on - strand, within GFF6332 at 127.248 kb on - strand, within GFF6332 at 127.248 kb on - strand, within GFF6332 at 127.248 kb on - strand, within GFF6332 at 127.248 kb on - strand, within GFF6332 at 127.248 kb on - strand, within GFF6332 at 127.248 kb on - strand, within GFF6332 at 127.248 kb on - strand, within GFF6332 at 127.248 kb on - strand, within GFF6332 at 127.248 kb on - strand, within GFF6332 at 127.248 kb on - strand, within GFF6332 at 127.469 kb on + strand, within GFF6332 at 127.469 kb on + strand, within GFF6332 at 127.470 kb on - strand, within GFF6332 at 127.470 kb on - strand, within GFF6332 at 127.470 kb on - strand, within GFF6332 at 127.491 kb on - strand, within GFF6332 at 127.613 kb on + strand, within GFF6332 at 127.613 kb on + strand, within GFF6332 at 127.613 kb on + strand, within GFF6332 at 127.613 kb on + strand, within GFF6332 at 127.614 kb on - strand, within GFF6332 at 127.614 kb on - strand, within GFF6332 at 127.614 kb on - strand, within GFF6332 at 127.614 kb on - strand, within GFF6332 at 127.646 kb on + strand, within GFF6332 at 127.646 kb on + strand, within GFF6332 at 127.646 kb on + strand, within GFF6332 at 127.646 kb on + strand, within GFF6332 at 127.646 kb on + strand, within GFF6332 at 127.646 kb on + strand, within GFF6332 at 127.647 kb on - strand, within GFF6332 at 127.647 kb on - strand, within GFF6332 at 127.874 kb on + strand, within GFF6332 at 127.874 kb on + strand, within GFF6332 at 127.874 kb on + strand, within GFF6332 at 127.874 kb on + strand, within GFF6332 at 127.874 kb on + strand, within GFF6332 at 127.875 kb on - strand, within GFF6332 at 127.875 kb on - strand, within GFF6332 at 127.875 kb on - strand, within GFF6332 at 127.875 kb on - strand, within GFF6332 at 127.875 kb on - strand, within GFF6332 at 127.875 kb on - strand, within GFF6332 at 127.937 kb on + strand, within GFF6332 at 127.937 kb on + strand, within GFF6332 at 127.937 kb on + strand, within GFF6332 at 128.054 kb on + strand, within GFF6332 at 128.054 kb on + strand, within GFF6332 at 128.055 kb on - strand, within GFF6332 at 128.055 kb on - strand, within GFF6332 at 128.387 kb on + strand at 128.686 kb on + strand, within GFF6333 at 128.687 kb on - strand, within GFF6333 at 128.687 kb on - strand, within GFF6333 at 128.687 kb on - strand, within GFF6333 at 129.004 kb on + strand, within GFF6333 at 129.004 kb on + strand, within GFF6333 at 129.005 kb on - strand, within GFF6333 at 129.005 kb on - strand, within GFF6333 at 129.005 kb on - strand, within GFF6333 at 129.005 kb on - strand, within GFF6333 at 129.178 kb on + strand, within GFF6333 at 129.178 kb on + strand, within GFF6333 at 129.178 kb on + strand, within GFF6333 at 129.178 kb on + strand, within GFF6333 at 129.178 kb on + strand, within GFF6333 at 129.178 kb on + strand, within GFF6333 at 129.178 kb on + strand, within GFF6333 at 129.179 kb on - strand, within GFF6333 at 129.179 kb on - strand, within GFF6333 at 129.179 kb on - strand, within GFF6333 at 129.179 kb on - strand, within GFF6333 at 129.179 kb on - strand, within GFF6333 at 129.236 kb on - strand, within GFF6333 at 129.265 kb on + strand, within GFF6333 at 129.265 kb on + strand, within GFF6333 at 129.265 kb on + strand, within GFF6333 at 129.265 kb on + strand, within GFF6333 at 129.266 kb on - strand, within GFF6333 at 129.266 kb on - strand, within GFF6333 at 129.266 kb on - strand, within GFF6333 at 129.340 kb on + strand, within GFF6333
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 125,807 + GFF6331 0.21 +0.6 125,807 + GFF6331 0.21 +0.2 125,807 + GFF6331 0.21 -2.2 125,807 + GFF6331 0.21 -0.9 125,807 + GFF6331 0.21 -0.8 125,807 + GFF6331 0.21 -1.4 125,807 + GFF6331 0.21 -0.1 125,807 + GFF6331 0.21 -0.2 125,808 - GFF6331 0.21 -0.6 125,808 - GFF6331 0.21 -0.2 125,808 - GFF6331 0.21 -0.2 125,808 - GFF6331 0.21 -0.2 125,808 - GFF6331 0.21 +1.0 125,808 - GFF6331 0.21 -0.1 125,808 - GFF6331 0.21 +0.1 125,920 + GFF6331 0.32 +1.1 125,920 + GFF6331 0.32 -0.8 125,920 + GFF6331 0.32 +0.5 125,920 + GFF6331 0.32 -0.1 125,920 + GFF6331 0.32 -1.3 125,921 - GFF6331 0.32 +0.1 125,921 - GFF6331 0.32 +0.2 125,921 - GFF6331 0.32 -0.4 125,921 - GFF6331 0.32 -0.1 125,921 - GFF6331 0.32 -1.2 126,069 + GFF6331 0.46 +1.2 126,070 - GFF6331 0.46 -0.1 126,235 + GFF6331 0.61 -0.8 126,236 - GFF6331 0.61 -0.1 126,318 - GFF6331 0.69 +0.8 126,318 - GFF6331 0.69 +0.8 126,448 + GFF6331 0.81 -0.2 126,448 + GFF6331 0.81 -1.3 126,448 + GFF6331 0.81 -0.5 126,449 - GFF6331 0.81 +0.8 126,449 - GFF6331 0.81 -0.4 126,449 - GFF6331 0.81 +0.6 126,449 - GFF6331 0.81 -0.2 126,469 + GFF6331 0.83 -0.8 126,469 + GFF6331 0.83 -0.2 126,692 - -1.2 126,803 + -0.2 126,803 + +0.1 126,803 + +0.2 126,803 + -1.5 126,804 - -0.7 126,812 + +0.0 126,812 + +2.2 126,812 + +0.2 126,813 - -0.6 126,813 - +0.8 127,145 + GFF6332 0.24 -1.3 127,145 + GFF6332 0.24 -0.2 127,145 + GFF6332 0.24 -0.3 127,145 + GFF6332 0.24 +0.8 127,145 + GFF6332 0.24 -0.3 127,145 + GFF6332 0.24 +0.2 127,145 + GFF6332 0.24 +0.7 127,145 + GFF6332 0.24 +1.2 127,145 + GFF6332 0.24 -0.2 127,146 - GFF6332 0.24 -0.1 127,146 - GFF6332 0.24 -0.3 127,146 - GFF6332 0.24 -0.3 127,146 - GFF6332 0.24 -0.0 127,244 + GFF6332 0.29 +0.1 127,244 + GFF6332 0.29 -0.2 127,244 + GFF6332 0.29 -0.2 127,244 + GFF6332 0.29 -0.8 127,244 + GFF6332 0.29 +0.5 127,244 + GFF6332 0.29 +0.8 127,245 - GFF6332 0.30 +0.2 127,245 - GFF6332 0.30 -0.6 127,245 - GFF6332 0.30 +1.0 127,247 + GFF6332 0.30 -1.0 127,247 + GFF6332 0.30 -0.6 127,248 - GFF6332 0.30 -1.4 127,248 - GFF6332 0.30 -1.2 127,248 - GFF6332 0.30 -0.8 127,248 - GFF6332 0.30 +0.5 127,248 - GFF6332 0.30 -0.0 127,248 - GFF6332 0.30 +0.6 127,248 - GFF6332 0.30 +0.4 127,248 - GFF6332 0.30 -0.0 127,248 - GFF6332 0.30 +1.8 127,248 - GFF6332 0.30 +0.7 127,248 - GFF6332 0.30 +0.8 127,248 - GFF6332 0.30 -0.2 127,469 + GFF6332 0.43 -1.9 127,469 + GFF6332 0.43 -0.4 127,470 - GFF6332 0.43 +0.2 127,470 - GFF6332 0.43 -1.4 127,470 - GFF6332 0.43 -1.2 127,491 - GFF6332 0.44 +0.4 127,613 + GFF6332 0.51 -1.2 127,613 + GFF6332 0.51 +0.4 127,613 + GFF6332 0.51 +0.4 127,613 + GFF6332 0.51 -0.5 127,614 - GFF6332 0.51 +0.7 127,614 - GFF6332 0.51 -0.5 127,614 - GFF6332 0.51 -0.8 127,614 - GFF6332 0.51 +0.1 127,646 + GFF6332 0.53 +1.4 127,646 + GFF6332 0.53 -1.6 127,646 + GFF6332 0.53 +0.2 127,646 + GFF6332 0.53 +0.3 127,646 + GFF6332 0.53 -1.4 127,646 + GFF6332 0.53 +1.2 127,647 - GFF6332 0.53 +1.0 127,647 - GFF6332 0.53 -0.1 127,874 + GFF6332 0.67 +2.4 127,874 + GFF6332 0.67 +1.1 127,874 + GFF6332 0.67 +2.0 127,874 + GFF6332 0.67 -0.3 127,874 + GFF6332 0.67 -0.4 127,875 - GFF6332 0.67 -0.9 127,875 - GFF6332 0.67 -0.3 127,875 - GFF6332 0.67 +0.0 127,875 - GFF6332 0.67 -0.3 127,875 - GFF6332 0.67 +0.4 127,875 - GFF6332 0.67 +1.0 127,937 + GFF6332 0.71 -1.3 127,937 + GFF6332 0.71 -0.2 127,937 + GFF6332 0.71 -0.5 128,054 + GFF6332 0.77 +0.9 128,054 + GFF6332 0.77 +0.1 128,055 - GFF6332 0.78 +1.1 128,055 - GFF6332 0.78 -0.2 128,387 + -0.3 128,686 + GFF6333 0.11 -0.3 128,687 - GFF6333 0.11 -1.5 128,687 - GFF6333 0.11 -0.6 128,687 - GFF6333 0.11 -0.0 129,004 + GFF6333 0.26 +0.1 129,004 + GFF6333 0.26 -1.6 129,005 - GFF6333 0.26 +1.3 129,005 - GFF6333 0.26 +0.2 129,005 - GFF6333 0.26 -0.1 129,005 - GFF6333 0.26 -1.2 129,178 + GFF6333 0.34 +0.7 129,178 + GFF6333 0.34 +0.4 129,178 + GFF6333 0.34 -1.0 129,178 + GFF6333 0.34 -2.0 129,178 + GFF6333 0.34 -1.0 129,178 + GFF6333 0.34 -1.2 129,178 + GFF6333 0.34 -0.4 129,179 - GFF6333 0.34 -2.0 129,179 - GFF6333 0.34 -1.3 129,179 - GFF6333 0.34 -1.2 129,179 - GFF6333 0.34 -0.8 129,179 - GFF6333 0.34 -0.3 129,236 - GFF6333 0.37 +1.0 129,265 + GFF6333 0.38 -0.8 129,265 + GFF6333 0.38 +1.1 129,265 + GFF6333 0.38 +0.1 129,265 + GFF6333 0.38 -2.3 129,266 - GFF6333 0.38 -0.5 129,266 - GFF6333 0.38 +0.1 129,266 - GFF6333 0.38 -0.5 129,340 + GFF6333 0.41 +0.9
Or see this region's nucleotide sequence