Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6299 and GFF6300 are separated by 4 nucleotides GFF6300 and GFF6301 overlap by 8 nucleotides
GFF6299 - BUG/TctC family periplasmic protein, at 88,087 to 89,067
GFF6299
GFF6300 - CaiB/BaiF family protein, at 89,072 to 90,262
GFF6300
GFF6301 - Pyruvate:Oxaloacetate transcarboxylase domain protein, at 90,255 to 91,193
GFF6301
Position (kb)
89
90
91 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 88.088 kb on + strand at 88.089 kb on - strand at 88.089 kb on - strand at 88.151 kb on + strand at 88.151 kb on + strand at 88.151 kb on + strand at 88.151 kb on + strand at 88.151 kb on + strand at 88.151 kb on + strand at 88.151 kb on + strand at 88.151 kb on + strand at 88.151 kb on + strand at 88.151 kb on + strand at 88.151 kb on + strand at 88.152 kb on - strand at 88.152 kb on - strand at 88.152 kb on - strand at 88.152 kb on - strand at 88.152 kb on - strand at 88.152 kb on - strand at 88.152 kb on - strand at 88.152 kb on - strand at 88.152 kb on - strand at 88.152 kb on - strand at 88.152 kb on - strand at 88.152 kb on - strand at 88.152 kb on - strand at 88.152 kb on - strand at 88.152 kb on - strand at 88.331 kb on + strand, within GFF6299 at 88.331 kb on + strand, within GFF6299 at 88.331 kb on + strand, within GFF6299 at 88.331 kb on + strand, within GFF6299 at 88.331 kb on + strand, within GFF6299 at 88.331 kb on + strand, within GFF6299 at 88.332 kb on - strand, within GFF6299 at 88.332 kb on - strand, within GFF6299 at 88.332 kb on - strand, within GFF6299 at 88.332 kb on - strand, within GFF6299 at 88.332 kb on - strand, within GFF6299 at 88.332 kb on - strand, within GFF6299 at 88.332 kb on - strand, within GFF6299 at 88.332 kb on - strand, within GFF6299 at 88.332 kb on - strand, within GFF6299 at 88.332 kb on - strand, within GFF6299 at 88.332 kb on - strand, within GFF6299 at 88.454 kb on + strand, within GFF6299 at 88.454 kb on + strand, within GFF6299 at 88.454 kb on + strand, within GFF6299 at 88.454 kb on + strand, within GFF6299 at 88.454 kb on + strand, within GFF6299 at 88.455 kb on - strand, within GFF6299 at 88.455 kb on - strand, within GFF6299 at 88.455 kb on - strand, within GFF6299 at 88.455 kb on - strand, within GFF6299 at 88.455 kb on - strand, within GFF6299 at 88.455 kb on - strand, within GFF6299 at 88.455 kb on - strand, within GFF6299 at 88.455 kb on - strand, within GFF6299 at 88.455 kb on - strand, within GFF6299 at 88.455 kb on - strand, within GFF6299 at 88.455 kb on - strand, within GFF6299 at 88.505 kb on + strand, within GFF6299 at 88.505 kb on + strand, within GFF6299 at 88.505 kb on + strand, within GFF6299 at 88.505 kb on + strand, within GFF6299 at 88.505 kb on + strand, within GFF6299 at 88.506 kb on - strand, within GFF6299 at 88.506 kb on - strand, within GFF6299 at 88.506 kb on - strand, within GFF6299 at 88.506 kb on - strand, within GFF6299 at 88.506 kb on - strand, within GFF6299 at 88.506 kb on - strand, within GFF6299 at 88.506 kb on - strand, within GFF6299 at 88.506 kb on - strand, within GFF6299 at 88.506 kb on - strand, within GFF6299 at 88.506 kb on - strand, within GFF6299 at 88.506 kb on - strand, within GFF6299 at 88.506 kb on - strand, within GFF6299 at 88.506 kb on - strand, within GFF6299 at 88.649 kb on + strand, within GFF6299 at 88.649 kb on + strand, within GFF6299 at 88.649 kb on + strand, within GFF6299 at 88.649 kb on + strand, within GFF6299 at 88.649 kb on + strand, within GFF6299 at 88.649 kb on + strand, within GFF6299 at 88.650 kb on - strand, within GFF6299 at 88.650 kb on - strand, within GFF6299 at 88.650 kb on - strand, within GFF6299 at 88.650 kb on - strand, within GFF6299 at 88.730 kb on + strand, within GFF6299 at 88.730 kb on + strand, within GFF6299 at 88.731 kb on - strand, within GFF6299 at 88.731 kb on - strand, within GFF6299 at 88.731 kb on - strand, within GFF6299 at 88.796 kb on + strand, within GFF6299 at 88.796 kb on + strand, within GFF6299 at 88.796 kb on + strand, within GFF6299 at 88.796 kb on + strand, within GFF6299 at 88.796 kb on + strand, within GFF6299 at 88.796 kb on + strand, within GFF6299 at 88.796 kb on + strand, within GFF6299 at 88.797 kb on - strand, within GFF6299 at 88.797 kb on - strand, within GFF6299 at 88.797 kb on - strand, within GFF6299 at 88.797 kb on - strand, within GFF6299 at 88.797 kb on - strand, within GFF6299 at 88.833 kb on + strand, within GFF6299 at 88.946 kb on + strand, within GFF6299 at 88.947 kb on - strand, within GFF6299 at 88.947 kb on - strand, within GFF6299 at 88.947 kb on - strand, within GFF6299 at 88.947 kb on - strand, within GFF6299 at 89.127 kb on + strand at 89.127 kb on + strand at 89.128 kb on - strand at 89.568 kb on + strand, within GFF6300 at 89.568 kb on + strand, within GFF6300 at 89.568 kb on + strand, within GFF6300 at 89.569 kb on - strand, within GFF6300 at 89.569 kb on - strand, within GFF6300 at 89.569 kb on - strand, within GFF6300 at 89.569 kb on - strand, within GFF6300 at 89.569 kb on - strand, within GFF6300 at 89.569 kb on - strand, within GFF6300 at 89.569 kb on - strand, within GFF6300 at 89.569 kb on - strand, within GFF6300 at 89.569 kb on - strand, within GFF6300 at 89.569 kb on - strand, within GFF6300 at 89.818 kb on - strand, within GFF6300 at 89.818 kb on - strand, within GFF6300 at 89.818 kb on - strand, within GFF6300 at 89.818 kb on - strand, within GFF6300 at 89.818 kb on - strand, within GFF6300 at 89.818 kb on - strand, within GFF6300 at 89.818 kb on - strand, within GFF6300 at 89.818 kb on - strand, within GFF6300 at 89.818 kb on - strand, within GFF6300 at 89.818 kb on - strand, within GFF6300 at 89.818 kb on - strand, within GFF6300 at 89.818 kb on - strand, within GFF6300 at 89.818 kb on - strand, within GFF6300 at 89.871 kb on + strand, within GFF6300 at 89.871 kb on + strand, within GFF6300 at 89.871 kb on + strand, within GFF6300 at 89.904 kb on + strand, within GFF6300 at 89.904 kb on + strand, within GFF6300 at 89.904 kb on + strand, within GFF6300 at 89.904 kb on + strand, within GFF6300 at 89.904 kb on + strand, within GFF6300 at 89.904 kb on + strand, within GFF6300 at 89.904 kb on + strand, within GFF6300 at 89.904 kb on + strand, within GFF6300 at 89.904 kb on + strand, within GFF6300 at 89.904 kb on + strand, within GFF6300 at 89.904 kb on + strand, within GFF6300 at 89.904 kb on + strand, within GFF6300 at 89.904 kb on + strand, within GFF6300 at 89.904 kb on + strand, within GFF6300 at 89.904 kb on + strand, within GFF6300 at 89.904 kb on + strand, within GFF6300 at 89.904 kb on + strand, within GFF6300 at 89.905 kb on - strand, within GFF6300 at 89.905 kb on - strand, within GFF6300 at 89.905 kb on - strand, within GFF6300 at 89.905 kb on - strand, within GFF6300 at 89.905 kb on - strand, within GFF6300 at 89.905 kb on - strand, within GFF6300 at 89.905 kb on - strand, within GFF6300 at 89.905 kb on - strand, within GFF6300 at 89.905 kb on - strand, within GFF6300 at 89.905 kb on - strand, within GFF6300 at 89.905 kb on - strand, within GFF6300 at 89.905 kb on - strand, within GFF6300 at 89.905 kb on - strand, within GFF6300 at 89.905 kb on - strand, within GFF6300 at 89.905 kb on - strand, within GFF6300 at 89.905 kb on - strand, within GFF6300 at 89.940 kb on + strand, within GFF6300 at 89.940 kb on + strand, within GFF6300 at 89.941 kb on - strand, within GFF6300 at 89.941 kb on - strand, within GFF6300 at 89.941 kb on - strand, within GFF6300 at 90.237 kb on + strand at 90.237 kb on + strand at 90.237 kb on + strand at 90.237 kb on + strand at 90.238 kb on - strand at 90.322 kb on + strand at 90.322 kb on + strand at 90.323 kb on - strand at 90.323 kb on - strand at 90.323 kb on - strand at 90.466 kb on + strand, within GFF6301 at 90.466 kb on + strand, within GFF6301 at 90.467 kb on - strand, within GFF6301 at 90.467 kb on - strand, within GFF6301 at 90.467 kb on - strand, within GFF6301 at 90.646 kb on + strand, within GFF6301 at 90.646 kb on + strand, within GFF6301 at 90.646 kb on + strand, within GFF6301 at 90.646 kb on + strand, within GFF6301 at 90.646 kb on + strand, within GFF6301 at 90.646 kb on + strand, within GFF6301 at 90.646 kb on + strand, within GFF6301 at 90.647 kb on - strand, within GFF6301 at 91.084 kb on + strand, within GFF6301 at 91.084 kb on + strand, within GFF6301 at 91.084 kb on + strand, within GFF6301 at 91.085 kb on - strand, within GFF6301 at 91.085 kb on - strand, within GFF6301 at 91.085 kb on - strand, within GFF6301
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 88,088 + -2.1 88,089 - -0.5 88,089 - +0.8 88,151 + +0.1 88,151 + -2.0 88,151 + -0.0 88,151 + -0.2 88,151 + +0.6 88,151 + -0.8 88,151 + -0.5 88,151 + +0.4 88,151 + -1.8 88,151 + -1.3 88,151 + -0.8 88,152 - -0.8 88,152 - +0.3 88,152 - -0.3 88,152 - -0.5 88,152 - +0.4 88,152 - +0.0 88,152 - +0.0 88,152 - -0.8 88,152 - -0.5 88,152 - -0.8 88,152 - -0.5 88,152 - +0.4 88,152 - -1.0 88,152 - +1.0 88,152 - +0.5 88,331 + GFF6299 0.25 -0.8 88,331 + GFF6299 0.25 -0.6 88,331 + GFF6299 0.25 +3.8 88,331 + GFF6299 0.25 +0.3 88,331 + GFF6299 0.25 +0.5 88,331 + GFF6299 0.25 -0.5 88,332 - GFF6299 0.25 +0.6 88,332 - GFF6299 0.25 -0.7 88,332 - GFF6299 0.25 -0.1 88,332 - GFF6299 0.25 -0.1 88,332 - GFF6299 0.25 -1.9 88,332 - GFF6299 0.25 -0.3 88,332 - GFF6299 0.25 -0.2 88,332 - GFF6299 0.25 -0.0 88,332 - GFF6299 0.25 +0.3 88,332 - GFF6299 0.25 -1.6 88,332 - GFF6299 0.25 +0.1 88,454 + GFF6299 0.37 -0.1 88,454 + GFF6299 0.37 -0.3 88,454 + GFF6299 0.37 +1.2 88,454 + GFF6299 0.37 +0.1 88,454 + GFF6299 0.37 +0.8 88,455 - GFF6299 0.38 -0.5 88,455 - GFF6299 0.38 -0.6 88,455 - GFF6299 0.38 -1.2 88,455 - GFF6299 0.38 -0.8 88,455 - GFF6299 0.38 +0.2 88,455 - GFF6299 0.38 +0.4 88,455 - GFF6299 0.38 -0.4 88,455 - GFF6299 0.38 +0.5 88,455 - GFF6299 0.38 +0.0 88,455 - GFF6299 0.38 -0.3 88,455 - GFF6299 0.38 +1.2 88,505 + GFF6299 0.43 -0.9 88,505 + GFF6299 0.43 +0.2 88,505 + GFF6299 0.43 +0.0 88,505 + GFF6299 0.43 -0.0 88,505 + GFF6299 0.43 -0.0 88,506 - GFF6299 0.43 +0.3 88,506 - GFF6299 0.43 -0.8 88,506 - GFF6299 0.43 -2.8 88,506 - GFF6299 0.43 -0.4 88,506 - GFF6299 0.43 -0.2 88,506 - GFF6299 0.43 -0.3 88,506 - GFF6299 0.43 -0.2 88,506 - GFF6299 0.43 -0.6 88,506 - GFF6299 0.43 +0.1 88,506 - GFF6299 0.43 -1.5 88,506 - GFF6299 0.43 +1.2 88,506 - GFF6299 0.43 -0.2 88,506 - GFF6299 0.43 +0.1 88,649 + GFF6299 0.57 -1.8 88,649 + GFF6299 0.57 +0.1 88,649 + GFF6299 0.57 -0.4 88,649 + GFF6299 0.57 +0.5 88,649 + GFF6299 0.57 +0.3 88,649 + GFF6299 0.57 -0.3 88,650 - GFF6299 0.57 -0.2 88,650 - GFF6299 0.57 +0.1 88,650 - GFF6299 0.57 -0.1 88,650 - GFF6299 0.57 +0.1 88,730 + GFF6299 0.66 +0.2 88,730 + GFF6299 0.66 +0.4 88,731 - GFF6299 0.66 -0.2 88,731 - GFF6299 0.66 +0.1 88,731 - GFF6299 0.66 -1.3 88,796 + GFF6299 0.72 -0.8 88,796 + GFF6299 0.72 -1.3 88,796 + GFF6299 0.72 -1.1 88,796 + GFF6299 0.72 -0.5 88,796 + GFF6299 0.72 -0.6 88,796 + GFF6299 0.72 +1.4 88,796 + GFF6299 0.72 +0.7 88,797 - GFF6299 0.72 -1.1 88,797 - GFF6299 0.72 +0.2 88,797 - GFF6299 0.72 +0.4 88,797 - GFF6299 0.72 -0.1 88,797 - GFF6299 0.72 -0.1 88,833 + GFF6299 0.76 -0.5 88,946 + GFF6299 0.88 -0.8 88,947 - GFF6299 0.88 -3.3 88,947 - GFF6299 0.88 +0.5 88,947 - GFF6299 0.88 +0.2 88,947 - GFF6299 0.88 -0.0 89,127 + -0.4 89,127 + -1.0 89,128 - -0.2 89,568 + GFF6300 0.42 +1.2 89,568 + GFF6300 0.42 +0.6 89,568 + GFF6300 0.42 -0.1 89,569 - GFF6300 0.42 +0.4 89,569 - GFF6300 0.42 -0.2 89,569 - GFF6300 0.42 +0.6 89,569 - GFF6300 0.42 -0.4 89,569 - GFF6300 0.42 +0.8 89,569 - GFF6300 0.42 +0.1 89,569 - GFF6300 0.42 +0.2 89,569 - GFF6300 0.42 +1.2 89,569 - GFF6300 0.42 -0.5 89,569 - GFF6300 0.42 -1.3 89,818 - GFF6300 0.63 +0.2 89,818 - GFF6300 0.63 +0.5 89,818 - GFF6300 0.63 -1.3 89,818 - GFF6300 0.63 +0.0 89,818 - GFF6300 0.63 +1.1 89,818 - GFF6300 0.63 -0.6 89,818 - GFF6300 0.63 -0.3 89,818 - GFF6300 0.63 -1.3 89,818 - GFF6300 0.63 +1.3 89,818 - GFF6300 0.63 -0.3 89,818 - GFF6300 0.63 -0.5 89,818 - GFF6300 0.63 -0.2 89,818 - GFF6300 0.63 -0.0 89,871 + GFF6300 0.67 -0.2 89,871 + GFF6300 0.67 -1.6 89,871 + GFF6300 0.67 +0.8 89,904 + GFF6300 0.70 -0.2 89,904 + GFF6300 0.70 +0.8 89,904 + GFF6300 0.70 -1.9 89,904 + GFF6300 0.70 -0.4 89,904 + GFF6300 0.70 +0.5 89,904 + GFF6300 0.70 +0.5 89,904 + GFF6300 0.70 +0.8 89,904 + GFF6300 0.70 +0.2 89,904 + GFF6300 0.70 -0.0 89,904 + GFF6300 0.70 +0.2 89,904 + GFF6300 0.70 -1.0 89,904 + GFF6300 0.70 -0.2 89,904 + GFF6300 0.70 -0.4 89,904 + GFF6300 0.70 -1.6 89,904 + GFF6300 0.70 +0.0 89,904 + GFF6300 0.70 +1.5 89,904 + GFF6300 0.70 -1.9 89,905 - GFF6300 0.70 -0.7 89,905 - GFF6300 0.70 +0.1 89,905 - GFF6300 0.70 -0.5 89,905 - GFF6300 0.70 -0.7 89,905 - GFF6300 0.70 +0.0 89,905 - GFF6300 0.70 +0.4 89,905 - GFF6300 0.70 -0.1 89,905 - GFF6300 0.70 -1.3 89,905 - GFF6300 0.70 +0.4 89,905 - GFF6300 0.70 -0.5 89,905 - GFF6300 0.70 +0.3 89,905 - GFF6300 0.70 +0.6 89,905 - GFF6300 0.70 -0.4 89,905 - GFF6300 0.70 -1.0 89,905 - GFF6300 0.70 -0.7 89,905 - GFF6300 0.70 -1.3 89,940 + GFF6300 0.73 +0.0 89,940 + GFF6300 0.73 -1.7 89,941 - GFF6300 0.73 +0.3 89,941 - GFF6300 0.73 -0.5 89,941 - GFF6300 0.73 -0.5 90,237 + -0.3 90,237 + -0.3 90,237 + -0.8 90,237 + +1.6 90,238 - -0.6 90,322 + -0.3 90,322 + -0.0 90,323 - -1.0 90,323 - -1.2 90,323 - +0.3 90,466 + GFF6301 0.22 -1.0 90,466 + GFF6301 0.22 -1.0 90,467 - GFF6301 0.23 -1.0 90,467 - GFF6301 0.23 +0.0 90,467 - GFF6301 0.23 -0.4 90,646 + GFF6301 0.42 -0.3 90,646 + GFF6301 0.42 +0.2 90,646 + GFF6301 0.42 +0.9 90,646 + GFF6301 0.42 -0.9 90,646 + GFF6301 0.42 -0.0 90,646 + GFF6301 0.42 +0.2 90,646 + GFF6301 0.42 +0.4 90,647 - GFF6301 0.42 -1.0 91,084 + GFF6301 0.88 -0.7 91,084 + GFF6301 0.88 +0.3 91,084 + GFF6301 0.88 -0.3 91,085 - GFF6301 0.88 +0.4 91,085 - GFF6301 0.88 +0.8 91,085 - GFF6301 0.88 -0.1
Or see this region's nucleotide sequence